EC: 3.5.1.1

asparaginase (L-asparagine amidohydrolase)

enzyme lambda metabolic reaction experiment
uniprot:Q8VI04 [asparaginase(Enzyme) wildtype]

H2O + L_Aspartic_acid_beta_(7_amido_4_methylcoumarin) -> L_Aspartate + 7_Amino_4_methylcoumarin		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 1% ethylene glycol
PH: 7.5
Temperature: 37
uniprot:Q8GXG1 [K+]

L_Asparagine + H2O -> NH3 + L_Aspartate		 (( (Vmax * S) ) / (Km + S)) buffer: 42 mM sodium potassium phosphate, 0.19 mM NADH, 3.1 mM alpha-ketoglutarate, 3.5 U porcine heart malate dehydrogenase, 2.5 U porcine heart glutamate oxaloacetate transaminase
PH: 7.2
Temperature: 24
uniprot:Q8GXG1 [K+]

L_Aspartyl_beta_hydroxamate + H2O -> Hydroxylamine + L_Aspartate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl
PH: 7.3
Temperature: 37
uniprot:P38986 [asparaginase(Enzyme) wildtype ASP1]

H2O + L_Asparagine -> L_Aspartate + NH3		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM Tris/sulfate
PH: 8
Temperature: 35
uniprot:P38986 [asparaginase(Enzyme) wildtype ASP1]

D_Asparagine + H2O -> NH3 + D_Aspartate		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM Tris/sulfate
PH: 8
Temperature: 35
uniprot:P11163 [asparaginase(Enzyme) wildtype ASP2]

H2O + L_Asparagine -> L_Aspartate + NH3		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM potassium phosphate
PH: 7
Temperature: 25
uniprot:P11163 [asparaginase(Enzyme) wildtype ASP2]

H2O + L_Isoleucyl_L_asparagine -> L_Isoleucyl_L_aspartate + NH3		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM potassium phosphate
PH: 7
Temperature: 25
uniprot:P11163 [asparaginase(Enzyme) wildtype ASP2]

H2O + N_Acetyl_L_asparagine -> NH3 + N_Acetyl_L_aspartate		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM potassium phosphate
PH: 7
Temperature: 25
uniprot:P11163 [asparaginase(Enzyme) wildtype ASP2]

H2O + D_Asparagine -> D_Aspartate + NH3		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM potassium phosphate
PH: 7
Temperature: 25
uniprot:P11163 [asparaginase(Enzyme) wildtype ASP2]

H2O + L_Methionyl_L_asparagine -> L_Methionyl_L_aspartate + NH3		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM potassium phosphate
PH: 7
Temperature: 25
uniprot:P11163 [asparaginase(Enzyme) wildtype ASP2]

L_Valyl_L_asparagine + H2O -> NH3 + L_Valyl_L_aspartate		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM potassium phosphate
PH: 7
Temperature: 25
uniprot:P11163 [asparaginase(Enzyme) wildtype ASP2]

H2O + Glycyl_L_asparagine -> Glycyl_L_aspartate + NH3		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM potassium phosphate
PH: 7
Temperature: 25
uniprot:P11163 [asparaginase(Enzyme) wildtype ASP2]

H2O + Glycyl_D_asparagine -> NH3 + Glycyl_D_aspartate		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM potassium phosphate
PH: 7
Temperature: 25
uniprot:P11163 [asparaginase(Enzyme) wildtype ASP2]

H2O + N_Carbamyl_L_asparagine -> NH3 + N_Carbamyl_L_aspartate		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM potassium phosphate
PH: 7
Temperature: 25
uniprot:P11163 [asparaginase(Enzyme) wildtype ASP2]

L_Asparagine + H2O -> NH3 + L_Aspartate		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM potassium phosphate
PH: 7
Temperature: 35
uniprot:P38986 [asparaginase(Enzyme) wildtype ASP1]

L_Asparagine + H2O -> L_Aspartate + NH3		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM Tris/sulfate
PH: 8
Temperature: 35
uniprot:P11163 [asparaginase(Enzyme) wildtype ASP2]

H2O + D_Asparagine -> D_Aspartate + NH3		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM potassium phosphate
PH: 7
Temperature: 35

Pathways

pathway id name
BioCyc:HUMAN_ASPARAGINE-DEG1-PWY-1 asparagine degradation
BioCyc:META_ASPARAGINE-DEG1-PWY-1 L-asparagine degradation III (mammalian)
BioCyc:ECOL316407_ASPASN-PWY superpathway of aspartate and asparagine biosynthesis; interconversion of aspartate and asparagine
BioCyc:META_ASPARAGINE-DEG1-PWY L-asparagine degradation I
BioCyc:META_ASPASN-PWY superpathway of L-aspartate and L-asparagine biosynthesis
BioCyc:ECO_ASPARAGINE-DEG1-PWY L-asparagine degradation I
BioCyc:ECO_ASPASN-PWY superpathway of L-aspartate and L-asparagine biosynthesis
BioCyc:YEAST_ASPARAGINE-DEG1-PWY asparagine degradation
BioCyc:MOUSE_ASPARAGINE-DEG1-PWY asparagine degradation I
BioCyc:ECOO157_ASPARAGINE-DEG1-PWY asparagine degradation I
BioCyc:ECOO157_ASPASN-PWY superpathway of aspartate and asparagine biosynthesis; interconversion of aspartate and asparagine
BioCyc:BSUB_ASPARAGINE-DEG1-PWY L-asparagine degradation I
BioCyc:MTBH37RV_ASPARAGINE-DEG1-PWY L-asparagine degradation I
BioCyc:ECOL316407_ASPARAGINE-DEG1-PWY asparagine degradation I
PathBank:SMP0000833 Asparagine Biosynthesis
Reactome:R-BTA-8964208 Phenylalanine metabolism
Reactome:R-DME-8963691 Phenylalanine and tyrosine metabolism
Reactome:R-HSA-8963691 Phenylalanine and tyrosine metabolism
Reactome:R-RNO-8963693 Aspartate and asparagine metabolism
Reactome:R-RNO-8964208 Phenylalanine metabolism
Reactome:R-BTA-8963691 Phenylalanine and tyrosine metabolism
Reactome:R-DRE-8964208 Phenylalanine metabolism
Reactome:R-RNO-8963691 Phenylalanine and tyrosine metabolism
Reactome:R-MMU-8963693 Aspartate and asparagine metabolism
Reactome:R-MMU-8964208 Phenylalanine metabolism
Reactome:R-DRE-8963691 Phenylalanine and tyrosine metabolism
Reactome:R-DME-8964208 Phenylalanine metabolism
Reactome:R-MMU-8963691 Phenylalanine and tyrosine metabolism
Reactome:R-HSA-8963693 Aspartate and asparagine metabolism
Reactome:R-HSA-8964208 Phenylalanine metabolism
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-71291 Amino acid and derivative metabolism
Reactome:R-DRE-71291 Amino acid and derivative metabolism
Reactome:R-MMU-1430728 Metabolism
Reactome:R-DME-1430728 Metabolism
Reactome:R-DME-71291 Amino acid and derivative metabolism
Reactome:R-DRE-1430728 Metabolism
Reactome:R-HSA-71291 Amino acid and derivative metabolism
Reactome:R-MMU-71291 Amino acid and derivative metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-71291 Amino acid and derivative metabolism
Reactome:R-HSA-1430728 Metabolism
PathBank:SMP0000009 Ammonia Recycling
PathBank:SMP0087165 Aspartate Metabolism
PathBank:SMP0087177 Ammonia Recycling
PathBank:SMP0087369 Aspartate Metabolism
PathBank:SMP0000067 Aspartate Metabolism
PathBank:SMP0000175 Canavan Disease
PathBank:SMP0000192 Hypoacetylaspartia
PathBank:SMP0002206 Asparagine Metabolism
PathBank:SMP0087375 Ammonia Recycling