EC: 3.3.2.9

microsomal epoxide hydrolase (cis-stilbene-oxide hydrolase)

enzyme lambda metabolic reaction experiment
uniprot:P07099 [microsomal epoxide hydrolase(Enzyme) wildtype]

H2O + (1_Chloroethenyl)oxirane -> 3_Chloro_3_butene_1,2_diol		 (( (Vmax * A) ) / (KmA + A)) buffer: 0.1 M phosphate, 15 mM MgCl2, 0.1 mM EDTA, 0.8 mM Glucose 6-phosphate, 1 U/ml Glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 38
uniprot:P07099 [microsomal epoxide hydrolase(Enzyme) wildtype]

H2O + (1_Chloroethenyl)oxirane -> 3_Chloro_3_butene_1,2_diol		 (( (Vmax * A) ) / (KmA + A)) buffer: 0.1 M phosphate, 15 mM MgCl2, 0.1 mM EDTA, 0.8 mM Glucose 6-phosphate, 1 U/ml Glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 38
uniprot:P07687 [microsomal epoxide hydrolase(Enzyme) wildtype]

H2O + (1_Chloroethenyl)oxirane -> 3_Chloro_3_butene_1,2_diol		 (( (Vmax * A) ) / (KmA + A)) buffer: 0.1 M phosphate, 15 mM MgCl2, 0.1 mM EDTA, 0.8 mM Glucose 6-phosphate, 1 U/ml Glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 38
uniprot:P07687 [microsomal epoxide hydrolase(Enzyme) wildtype]

(1_Chloroethenyl)oxirane + H2O -> 3_Chloro_3_butene_1,2_diol		 (( (Vmax * A) ) / (KmA + A)) buffer: 0.1 M phosphate, 15 mM MgCl2, 0.1 mM EDTA, 0.8 mM Glucose 6-phosphate, 1 U/ml Glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 38
uniprot:P07687 [microsomal epoxide hydrolase(Enzyme) wildtype]

H2O + (1_Chloroethenyl)oxirane -> 3_Chloro_3_butene_1,2_diol		 (( (Vmax * A) ) / (KmA + A)) buffer: 0.1 M phosphate, 15 mM MgCl2, 0.1 mM EDTA, 0.8 mM Glucose 6-phosphate, 1 U/ml Glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 38
uniprot:P07687 [microsomal epoxide hydrolase(Enzyme) wildtype]

(1_Chloroethenyl)oxirane + H2O -> 3_Chloro_3_butene_1,2_diol		 (( (Vmax * A) ) / (KmA + A)) buffer: 0.1 M phosphate, 15 mM MgCl2, 0.1 mM EDTA, 0.8 mM Glucose 6-phosphate, 1 U/ml Glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 38
uniprot:Q9D379 [microsomal epoxide hydrolase(Enzyme) wildtype]

H2O + (1_Chloroethenyl)oxirane -> 3_Chloro_3_butene_1,2_diol		 (( (Vmax * A) ) / (KmA + A)) buffer: 0.1 M phosphate, 15 mM MgCl2, 0.1 mM EDTA, 0.8 mM Glucose 6-phosphate, 1 U/ml Glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 38
- [microsomal epoxide hydrolase(Enzyme) wildtype]

H2O + (1_Chloroethenyl)oxirane -> 3_Chloro_3_butene_1,2_diol		 (( (Vmax * A) ) / (KmA + A)) buffer: 0.1 M phosphate, 15 mM MgCl2, 0.1 mM EDTA, 0.8 mM Glucose 6-phosphate, 1 U/ml Glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 38
- [microsomal epoxide hydrolase(Enzyme) wildtype]

(1_Chloroethenyl)oxirane + H2O -> 3_Chloro_3_butene_1,2_diol		 (( (Vmax * A) ) / (KmA + A)) buffer: 0.1 M phosphate, 15 mM MgCl2, 0.1 mM EDTA, 0.8 mM Glucose 6-phosphate, 1 U/ml Glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 38
uniprot:Q9D379 [microsomal epoxide hydrolase(Enzyme) wildtype]

(1_Chloroethenyl)oxirane + H2O -> 3_Chloro_3_butene_1,2_diol		 (( (Vmax * A) ) / (KmA + A)) buffer: 0.1 M phosphate, 15 mM MgCl2, 0.1 mM EDTA, 0.8 mM Glucose 6-phosphate, 1 U/ml Glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 38

Pathways

pathway id name
Reactome:R-HSA-211859 Biological oxidations
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-211859 Biological oxidations
Reactome:R-RNO-211945 Phase I - Functionalization of compounds
Reactome:R-RNO-211859 Biological oxidations
Reactome:R-HSA-211945 Phase I - Functionalization of compounds
Reactome:R-MMU-211945 Phase I - Functionalization of compounds
Reactome:R-RNO-1430728 Metabolism
Reactome:R-HSA-1430728 Metabolism
WikiPathways:WP5238 Cholestasis
PathBank:SMP0000327 Phenytoin (Antiarrhythmic) Action Pathway
PathBank:SMP0000634 Carbamazepine Metabolism Pathway