EC: 3.2.1.55

non-reducing end alpha-L-arabinofuranosidase (alpha-L-arabinofuranoside non-reducing end alpha-L-arabinofuranosidase)

enzyme lambda metabolic reaction experiment
uniprot:Q9FGY1 [alpha-N-arabinofuranosidase(Enzyme) wildtype XYL1]

p_Nitrophenyl_beta_D_xylopyranoside + H2O -> p_Nitrophenol + D_Xylose		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.1 M acetate, 2 mM sodium azide
PH: 5
Temperature: 37
uniprot:Q9SG80 [alpha-N-arabinofuranosidase(Enzyme) wildtype ARAf]

H2O + p_Nitrophenyl_beta_D_xylopyranoside -> D_Xylose + p_Nitrophenol		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.1 M acetate, 2 mM sodium azide
PH: 5
Temperature: 37
uniprot:Q9ATV8 [alpha-N-arabinofuranosidase(Enzyme) wildtype]

4_Nitrophenyl_alpha_L_arabinofuranoside + H2O -> p_Nitrophenol + alpha_L_Arabinofuranose		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM sodium acetate, 4 mM NaN3, 160 µg/ml BSA
PH: 4.3
Temperature: 37
uniprot:Q9ATV8 [alpha-N-arabinofuranosidase(Enzyme) wildtype]

Arabinoxylan + H2O -> L_Arabinose + Arabinoxylan		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM sodium acetate, 4 mM NaN3, 160 µg/ml BSA
PH: 4.3
Temperature: 37
uniprot:Q2MJP5 [alpha-N-arabinofuranosidase(Enzyme) wildtype AraB]

4_Nitrophenyl_alpha_L_arabinofuranoside + H2O -> p_Nitrophenol + alpha_L_Arabinofuranose		 (( (Vmax * S) ) / (S + Km)) buffer: 50 mM phthalate-imidole
PH: 6.4
Temperature: 60
uniprot:A7LXT8 [alpha-N-arabinofuranosidase(Enzyme) wildtype BoGH43A His-tagged]

H2O + 4_Nitrophenyl_alpha_L_arabinofuranoside -> alpha_L_Arabinofuranose + p_Nitrophenol		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Phosphate
PH: 6
Temperature: 37
uniprot:A7LXT8 [alpha-N-arabinofuranosidase(Enzyme) wildtype BoGH43A His-tagged]

4_Nitrophenyl_alpha_L_arabinofuranoside + H2O -> p_Nitrophenol + alpha_L_Arabinofuranose		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Phosphate
PH: 6
Temperature: 37
uniprot:A7LXT8 [alpha-N-arabinofuranosidase(Enzyme) wildtype BoGH43A His-tagged]

p_Nitrophenyl_beta_D_xylopyranoside + H2O -> beta_D_Xylose + p_Nitrophenol		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Phosphate
PH: 6
Temperature: 37
uniprot:A7LXU0 [alpha-N-arabinofuranosidase(Enzyme) wildtype BoGH43B His-tagged]

Xyloglucan + H2O -> n_n_Oligoxyloglucan		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Phosphate
PH: 6
Temperature: 37
uniprot:A7LXT8 [alpha-N-arabinofuranosidase(Enzyme) wildtype BoGH43A His-tagged]

H2O + Xyloglucan -> n_n_Oligoxyloglucan		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Phosphate
PH: 6
Temperature: 37
uniprot:A5JTQ2 [alpha-N-arabinofuranosidase(Enzyme) wildtype]

4_Nitrophenyl_alpha_L_arabinofuranoside + H2O -> L_Arabinofuranose + p_Nitrophenol		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM sodium acetate
PH: 5
Temperature: 37
uniprot:A5JTQ2 [alpha-N-arabinofuranosidase(Enzyme) wildtype]

4_Nitrophenyl_alpha_L_arabinopyranoside + H2O -> alpha_L_Arabinose + p_Nitrophenol		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM sodium acetate
PH: 5
Temperature: 37
- [alpha-N-arabinofuranosidase(Enzyme) mutant N83Q/N202Q]

H2O + 4_Nitrophenyl_alpha_L_arabinofuranoside -> p_Nitrophenol + alpha_L_Arabinofuranose		 (( (V * S) ) / (Km + S)) buffer: 50 mM sodium acetate
PH: 4
Temperature: 37
- [alpha-N-arabinofuranosidase(Enzyme) wildtype]

4_Nitrophenyl_alpha_L_arabinofuranoside + H2O -> p_Nitrophenol + alpha_L_Arabinofuranose		 (( (V * S) ) / (Km + S)) buffer: 50 mM sodium acetate
PH: 4
Temperature: 37
- [alpha-N-arabinofuranosidase(Enzyme) mutant N83Q]

4_Nitrophenyl_alpha_L_arabinofuranoside + H2O -> alpha_L_Arabinofuranose + p_Nitrophenol		 (( (V * S) ) / (Km + S)) buffer: 50 mM sodium acetate
PH: 4
Temperature: 37
- [alpha-N-arabinofuranosidase(Enzyme) mutant N202Q]

H2O + 4_Nitrophenyl_alpha_L_arabinofuranoside -> p_Nitrophenol + alpha_L_Arabinofuranose		 (( (V * S) ) / (Km + S)) buffer: 50 mM sodium acetate
PH: 4
Temperature: 37
uniprot:Q841V6 [alpha-N-arabinofuranosidase(Enzyme) wildtype His-tagged]

4_Nitrophenyl_alpha_L_arabinofuranoside + H2O -> p_Nitrophenol + alpha_L_Arabinofuranose		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Potassium phosphate
PH: 6
Temperature: 45
uniprot:Q841V6 [alpha-N-arabinofuranosidase(Enzyme) wildtype His-tagged]

alpha_L_Arabinan + H2O -> alpha_L_Arabinan + L_Arabinose		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Potassium phosphate
PH: 6
Temperature: 45
uniprot:Q841V6 [alpha-N-arabinofuranosidase(Enzyme) wildtype His-tagged]

H2O + Arabinoxylan -> L_Arabinose + Arabinoxylan		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Potassium phosphate
PH: 6
Temperature: 45

Pathways

pathway id name
PlantCyc:ARA_PWY-6717 (1,4)-β-D-xylan degradation
PlantCyc:PLANT_PWY-6717 (1,4)-β-D-xylan degradation