EC: 3.2.1.37

xylan 1,4-beta-xylosidase (4-beta-D-xylan xylohydrolase)

enzyme lambda metabolic reaction experiment
uniprot:Q9FLG1 [xylan 1,4-beta-xylosidase(Enzyme) wildtype XYL4]

p_Nitrophenyl_beta_D_xylopyranoside + H2O -> p_Nitrophenol + D_Xylose		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.1 M acetate, 2 mM sodium azide
PH: 5
Temperature: 37
uniprot:V5N4G7 [xylan 1,4-beta-xylosidase(Enzyme) wildtype]

H2O + p_Nitrophenyl_beta_D_xylopyranoside -> beta_D_Xylose + p_Nitrophenol		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM sodium phosphate
PH: 7
Temperature: 60
uniprot:Q8X212 [xylan 1,4-beta-xylosidase(Enzyme) wildtype]

H2O + p_Nitrophenyl_beta_D_xylopyranoside -> p_Nitrophenol + beta_D_Xylose		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Citrate
PH: 4
Temperature: 40
- [xylan 1,4-beta-xylosidase(Enzyme) wildtype]

H2O + p_Nitrophenyl_beta_D_xylopyranoside -> beta_D_Xylose + p_Nitrophenol		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM phosphate
PH: 7
Temperature: 50
uniprot:Q8X212 [xylan 1,4-beta-xylosidase(Enzyme) wildtype]

Xylobiose + H2O -> 2 beta_D_Xylose		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Citrate
PH: 4
Temperature: 40
uniprot:Q92458 [xylan 1,4-beta-xylosidase(Enzyme) wildtype]

H2O + Xylohexaose -> Xylopentaose + beta_D_Xylose		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Citrate
PH: 4
Temperature: 40
uniprot:O00089 [xylan 1,4-beta-xylosidase(Enzyme) wildtype]

H2O + p_Nitrophenyl_beta_D_xylopyranoside -> beta_D_Xylose + p_Nitrophenol		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM sodium citrate
PH: 4
Temperature: 65
uniprot:O00089 [xylan 1,4-beta-xylosidase(Enzyme) wildtype]

p_Nitrophenyl_beta_D_xylopyranoside + H2O -> beta_D_Xylose + p_Nitrophenol		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Citrate
PH: 4.8
Temperature: 37
uniprot:Q92458 [xylan 1,4-beta-xylosidase(Enzyme) wildtype]

H2O + p_Nitrophenyl_beta_D_xylopyranoside -> p_Nitrophenol + beta_D_Xylose		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Citrate
PH: 4
Temperature: 40
uniprot:Q92458 [xylan 1,4-beta-xylosidase(Enzyme) wildtype]

Xylotetraose + H2O -> beta_D_Xylose + Xylotriose		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Citrate
PH: 4
Temperature: 40
uniprot:Q8X212 [xylan 1,4-beta-xylosidase(Enzyme) wildtype]

H2O + Xylohexaose -> beta_D_Xylose + Xylopentaose		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Citrate
PH: 4
Temperature: 40
uniprot:Q8X212 [xylan 1,4-beta-xylosidase(Enzyme) wildtype]

Xylotetraose + H2O -> Xylotriose + beta_D_Xylose		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Citrate
PH: 4
Temperature: 40
uniprot:Q92458 [xylan 1,4-beta-xylosidase(Enzyme) wildtype]

H2O + Xylopentaose -> beta_D_Xylose + Xylotetraose		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Citrate
PH: 4
Temperature: 40
uniprot:Q8X212 [xylan 1,4-beta-xylosidase(Enzyme) wildtype]

H2O + Xylopentaose -> Xylotetraose + beta_D_Xylose		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Citrate
PH: 4
Temperature: 40
uniprot:Q92458 [xylan 1,4-beta-xylosidase(Enzyme) wildtype]

Xylotriose + H2O -> beta_D_Xylose + Xylobiose		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Citrate
PH: 4
Temperature: 40
uniprot:Q8X212 [xylan 1,4-beta-xylosidase(Enzyme) wildtype]

Xylotriose + H2O -> beta_D_Xylose + Xylobiose		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Citrate
PH: 4
Temperature: 40
uniprot:Q92458 [xylan 1,4-beta-xylosidase(Enzyme) wildtype]

Xylobiose + H2O -> 2 beta_D_Xylose		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Citrate
PH: 4
Temperature: 40
uniprot:A5JTQ2 [xylan 1,4-beta-xylosidase(Enzyme) wildtype]

H2O + p_Nitrophenyl_beta_D_xylopyranoside -> beta_D_Xylose + p_Nitrophenol		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM sodium acetate
PH: 5
Temperature: 37
uniprot:D4AHT5 [xylan 1,4-beta-xylosidase(Enzyme) wildtype]

H2O + p_Nitrophenyl_beta_D_xylopyranoside -> beta_D_Xylose + p_Nitrophenol		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M acetate-HCl
PH: 3.5
Temperature: 70
uniprot:P36906 [xylan 1,4-beta-xylosidase(Enzyme) wildtype]

2,5_Dinitrophenyl_beta_D_xylopyranoside + H2O -> D_Xylose + 2,5_Dinitrophenol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 25 mM citrate, 25 mM phosphate, 0.1% BSA
PH: 5.5
Temperature: 37
uniprot:P36906 [xylan 1,4-beta-xylosidase(Enzyme) wildtype]

H2O + p_Nitrophenyl_beta_D_xylopyranoside -> p_Nitrophenol + D_Xylose		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 25 mM citrate, 25 mM phosphate, 0.1% BSA
PH: 5.5
Temperature: 37
uniprot:P36906 [xylan 1,4-beta-xylosidase(Enzyme) mutant E160A]

p_Nitrophenyl_beta_D_xylopyranoside + H2O -> D_Xylose + p_Nitrophenol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 25 mM citrate, 25 mM phosphate, 0.1% BSA
PH: 6.5
Temperature: 37
uniprot:P36906 [xylan 1,4-beta-xylosidase(Enzyme) wildtype]

H2O + 2_Nitrophenyl_beta_D_xylopyranoside -> D_Xylose + o_Nitrophenol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 25 mM citrate, 25 mM phosphate, 0.1% BSA
PH: 5.5
Temperature: 37
uniprot:P36906 [xylan 1,4-beta-xylosidase(Enzyme) mutant E160A]

H2O + 2_Nitrophenyl_beta_D_xylopyranoside -> D_Xylose + o_Nitrophenol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 25 mM citrate, 25 mM phosphate, 0.1% BSA
PH: 6.5
Temperature: 37
uniprot:P36906 [xylan 1,4-beta-xylosidase(Enzyme) wildtype]

3_Nitrophenyl_beta_D_xylopyranoside + H2O -> D_Xylose + 3_Nitrophenol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 25 mM citrate, 25 mM phosphate, 0.1% BSA
PH: 5.5
Temperature: 37
uniprot:P36906 [xylan 1,4-beta-xylosidase(Enzyme) mutant E160A]

3_Nitrophenyl_beta_D_xylopyranoside + H2O -> D_Xylose + 3_Nitrophenol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 25 mM citrate, 25 mM phosphate, 0.1% BSA
PH: 6.5
Temperature: 37
uniprot:P36906 [xylan 1,4-beta-xylosidase(Enzyme) mutant E160A]

2,5_Dinitrophenyl_beta_D_xylopyranoside + H2O -> 2,5_Dinitrophenol + D_Xylose		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 25 mM citrate, 25 mM phosphate, 0.1% BSA
PH: 6.5
Temperature: 37
uniprot:P36906 [xylan 1,4-beta-xylosidase(Enzyme) wildtype]

3,4_Dinitrophenyl_beta_D_xylopyranoside + H2O -> 3,4_Dinitrophenol + D_Xylose		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 25 mM citrate, 25 mM phosphate, 0.1% BSA
PH: 5.5
Temperature: 37
uniprot:P36906 [xylan 1,4-beta-xylosidase(Enzyme) mutant E160A]

H2O + 3,4_Dinitrophenyl_beta_D_xylopyranoside -> D_Xylose + 3,4_Dinitrophenol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 25 mM citrate, 25 mM phosphate, 0.1% BSA
PH: 6.5
Temperature: 37
uniprot:B5AK91 [xylan 1,4-beta-xylosidase(Enzyme) wildtype]

H2O + 3,4_Dimethylphenyl_beta_D_xylopyranoside -> 3,4_Dimethylphenol + beta_D_Xylose		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM sodium acetate, 1 mg/ml BSA
PH: 4.5
Temperature: 37
uniprot:B5AK91 [xylan 1,4-beta-xylosidase(Enzyme) wildtype]

H2O + 2_Naphthyl_beta_D_xylopyranoside -> beta_D_Xylose + beta_Naphthol		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM sodium acetate, 1 mg/ml BSA
PH: 4.5
Temperature: 37
uniprot:B5AK91 [xylan 1,4-beta-xylosidase(Enzyme) wildtype]

H2O + 4_o_Methoxyphenyl_beta_D_xylopyranoside -> 4_o_Methoxyphenol + beta_D_Xylose		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM sodium acetate, 1 mg/ml BSA
PH: 4.5
Temperature: 37
uniprot:B5AK91 [xylan 1,4-beta-xylosidase(Enzyme) wildtype]

4_Bromophenyl_beta_D_xylopyranoside + H2O -> beta_D_Xylose + 4_Bromophenol		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM sodium acetate, 1 mg/ml BSA
PH: 4.5
Temperature: 37
uniprot:B5AK91 [xylan 1,4-beta-xylosidase(Enzyme) wildtype]

p_Nitrophenyl_beta_D_xylopyranoside + H2O -> p_Nitrophenol + beta_D_Xylose		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM sodium acetate, 1 mg/ml BSA
PH: 4.5
Temperature: 37
uniprot:B5AK91 [xylan 1,4-beta-xylosidase(Enzyme) wildtype]

H2O + p_Nitrophenyl_beta_D_xylopyranoside -> beta_D_Xylose + p_Nitrophenol		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM sodium acetate, 1 mg/ml BSA
PH: 4.5
Temperature: 37
uniprot:B5AK91 [xylan 1,4-beta-xylosidase(Enzyme) wildtype]

H2O + p_Nitrophenyl_beta_D_xylopyranoside -> beta_D_Xylose + p_Nitrophenol		 (v0 / (1 + (( (( (Km * I) ) / Ki) ) / (Km + S)))) buffer: 50 mM sodium acetate, 1 mg/ml BSA
PH: 4.5
Temperature: 37
uniprot:B5AK91 [xylan 1,4-beta-xylosidase(Enzyme) wildtype]

H2O + p_Nitrophenyl_beta_D_xylopyranoside -> p_Nitrophenol + beta_D_Xylose		 (v0 / (1 + (( (( (Km * I) ) / Ki) ) / (Km + S)))) buffer: 50 mM sodium acetate, 1 mg/ml BSA
PH: 4.5
Temperature: 37
uniprot:B5AK91 [xylan 1,4-beta-xylosidase(Enzyme) wildtype]

p_Nitrophenyl_beta_D_xylopyranoside + H2O -> p_Nitrophenol + beta_D_Xylose		 (v0 / (1 + (( (( (Km * I) ) / Ki) ) / (Km + S)))) buffer: 50 mM sodium acetate, 1 mg/ml BSA
PH: 4.5
Temperature: 37
uniprot:B5AK91 [xylan 1,4-beta-xylosidase(Enzyme) wildtype]

p_Nitrophenyl_beta_D_glucoside + H2O -> p_Nitrophenol + beta_D_Glucose		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM sodium acetate, 1 mg/ml BSA
PH: 4.5
Temperature: 37
uniprot:B5AK91 [xylan 1,4-beta-xylosidase(Enzyme) wildtype]

H2O + 4_Nitrophenyl_alpha_L_arabinopyranoside -> alpha_L_Arabinose + p_Nitrophenol		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM sodium acetate, 1 mg/ml BSA
PH: 4.5
Temperature: 37
uniprot:B5AK91 [xylan 1,4-beta-xylosidase(Enzyme) wildtype]

H2O + 4_Methylumbelliferyl_beta_D_xylopyranoside -> beta_D_Xylose + 4_Methylumbelliferone		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM sodium acetate, 1 mg/ml BSA
PH: 4.5
Temperature: 37
uniprot:B5AK91 [xylan 1,4-beta-xylosidase(Enzyme) wildtype]

H2O + 3_Nitrophenyl_beta_D_xylopyranoside -> beta_D_Xylose + 3_Nitrophenol		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM sodium acetate, 1 mg/ml BSA
PH: 4.5
Temperature: 37
uniprot:B5AK91 [xylan 1,4-beta-xylosidase(Enzyme) wildtype]

H2O + 2,4_Dinitrophenyl_beta_D_xylopyranoside -> beta_D_Xylose + 2,4_Dinitrophenol		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM sodium acetate, 1 mg/ml BSA
PH: 4.5
Temperature: 37
uniprot:B5AK91 [xylan 1,4-beta-xylosidase(Enzyme) wildtype]

H2O + 2,5_Dinitrophenyl_beta_D_xylopyranoside -> 2,5_Dinitrophenol + beta_D_Xylose		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM sodium acetate, 1 mg/ml BSA
PH: 4.5
Temperature: 37
uniprot:O52575 [xylan 1,4-beta-xylosidase(Enzyme) mutant C246V]

p_Nitrophenyl_beta_D_xylopyranoside + H2O -> D_Xylose + p_Nitrophenol		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM sodium acetate
PH: 4.8
Temperature: 50
uniprot:O52575 [xylan 1,4-beta-xylosidase(Enzyme) wildtype]

p_Nitrophenyl_beta_D_xylopyranoside + H2O -> D_Xylose + p_Nitrophenol		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM sodium acetate
PH: 4.8
Temperature: 50
uniprot:O52575 [xylan 1,4-beta-xylosidase(Enzyme) mutant C101V]

H2O + p_Nitrophenyl_beta_D_xylopyranoside -> p_Nitrophenol + D_Xylose		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM sodium acetate
PH: 4.8
Temperature: 50
uniprot:O52575 [xylan 1,4-beta-xylosidase(Enzyme) mutant C286V]

p_Nitrophenyl_beta_D_xylopyranoside + H2O -> D_Xylose + p_Nitrophenol		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM sodium acetate
PH: 4.8
Temperature: 50
uniprot:O52575 [xylan 1,4-beta-xylosidase(Enzyme) mutant C413V]

H2O + p_Nitrophenyl_beta_D_xylopyranoside -> D_Xylose + p_Nitrophenol		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM sodium acetate
PH: 4.8
Temperature: 50

Pathways

pathway id name
BioCyc:META_PWY-6717 (1,4)-β-D-xylan degradation
PlantCyc:ARA_PWY-6717 (1,4)-β-D-xylan degradation
PlantCyc:PLANT_PWY-6717 (1,4)-β-D-xylan degradation