EC: 3.2.1.26

beta-fructofuranosidase (beta-D-fructofuranoside fructohydrolase)

enzyme lambda metabolic reaction experiment
- [beta-fructofuranosidase(Enzyme) wildtype]

H2O + Sucrose -> D_Glucose + D_Fructose		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM HEPES
PH: 7.2
Temperature: 30
- [beta-fructofuranosidase(Enzyme) wildtype]

Sucrose + H2O -> D_Fructose + D_Glucose		 (( (( (( (vol * Vmax9) ) / (1 + (Glc / Ki9Glc))) ) * Suc) ) / (( (Km9Suc * (1 + (Fru / Ki9Fru))) ) + Suc)) buffer: *
PH: 7
Temperature: 36
uniprot:B2NIA1 [beta-fructofuranosidase(Enzyme) wildtype]

Sucrose + H2O -> D_Fructose + D_Glucose		 (( (Vmax * S) ) / (Km + S)) buffer: 30 mM Acetate
PH: 4.5
Temperature: 30
uniprot:B2NIA0 [beta-fructofuranosidase(Enzyme) wildtype]

H2O + Sucrose -> D_Fructose + D_Glucose		 (( (Vmax * S) ) / (Km + S)) buffer: 30 mM Acetate
PH: 4.5
Temperature: 30
uniprot:P29001 [beta-fructofuranosidase(Enzyme) wildtype Invertase]

H2O + Sucrose -> beta_D_Fructose + alpha_D_Glucose		 (( (Vmax * S) ) / (Km + S)) buffer: 0.05 M Sodium cacodylate
PH: 5.5
Temperature: 25
- [beta-fructofuranosidase(Enzyme) wildtype]

Sucrose + H2O -> D_Fructose + D_Glucose		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Sodium acetate
PH: 5
Temperature: 50
- [beta-fructofuranosidase(Enzyme) wildtype]

Sucrose_6_phosphate + H2O -> beta_D_Fructose + alpha_D_Glucose_6_phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 10 mM MgCl2, 1 mM NADP+, 5 U/ml glucose 6-phosphate dehydrogenase
PH: 7
Temperature: 22
- [beta-fructofuranosidase(Enzyme) wildtype]

H2O + Sucrose -> alpha_D_Glucose + beta_D_Fructose		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 10 mM ATP, 10 mM MgCl2, 1 mM NADP+, 5 U/ml hexokinase, 5 U/ml glucose 6-phosphate dehydrogenase
PH: 7
Temperature: 22
uniprot:Q43866 [beta-fructofuranosidase(Enzyme) wildtype]

H2O + Sucrose -> D_Fructose + D_Glucose		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM sodium acetate, 0.02% (v/v) sodium azide
PH: 5
Temperature: 30
uniprot:Q43866 [beta-fructofuranosidase(Enzyme) mutant W20L]

H2O + Sucrose -> D_Fructose + D_Glucose		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM sodium acetate, 0.02% (v/v) sodium azide
PH: 5
Temperature: 30
uniprot:Q43866 [beta-fructofuranosidase(Enzyme) mutant E203A]

Sucrose + H2O -> D_Fructose + D_Glucose		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM sodium acetate, 0.02% (v/v) sodium azide
PH: 5
Temperature: 30
uniprot:Q43866 [beta-fructofuranosidase(Enzyme) mutant D239N]

H2O + Sucrose -> D_Fructose + D_Glucose		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM sodium acetate, 0.02% (v/v) sodium azide
PH: 5
Temperature: 30
uniprot:Q43866 [beta-fructofuranosidase(Enzyme) mutant D239F]

Sucrose + H2O -> D_Fructose + D_Glucose		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM sodium acetate, 0.02% (v/v) sodium azide
PH: 5
Temperature: 30
uniprot:Q43866 [beta-fructofuranosidase(Enzyme) mutant D23A]

Sucrose + H2O -> D_Glucose + D_Fructose		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM sodium acetate, 0.02% (v/v) sodium azide
PH: 5
Temperature: 30
uniprot:Q43866 [beta-fructofuranosidase(Enzyme) mutant K242L]

H2O + Sucrose -> D_Glucose + D_Fructose		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM sodium acetate, 0.02% (v/v) sodium azide
PH: 5
Temperature: 30
uniprot:Q43866 [beta-fructofuranosidase(Enzyme) mutant D239A]

H2O + Sucrose -> D_Fructose + D_Glucose		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM sodium acetate, 0.02% (v/v) sodium azide
PH: 5
Temperature: 30
uniprot:Q43866 [beta-fructofuranosidase(Enzyme) mutant W47L]

Sucrose + H2O -> D_Fructose + D_Glucose		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM sodium acetate, 0.02% (v/v) sodium azide
PH: 5
Temperature: 30
uniprot:Q43866 [beta-fructofuranosidase(Enzyme) mutant W82L]

Sucrose + H2O -> D_Glucose + D_Fructose		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM sodium acetate, 0.02% (v/v) sodium azide
PH: 5
Temperature: 30
uniprot:Q43866 [beta-fructofuranosidase(Enzyme) wildtype]

H2O + 1_Kestose -> Sucrose + D_Fructose		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM sodium acetate, 0.02% (v/v) sodium azide
PH: 5
Temperature: 30
uniprot:Q43866 [beta-fructofuranosidase(Enzyme) mutant D239A]

1_Kestose + H2O -> D_Fructose + Sucrose		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM sodium acetate, 0.02% (v/v) sodium azide
PH: 5
Temperature: 30
uniprot:Q43866 [beta-fructofuranosidase(Enzyme) mutant D239F]

1_Kestose + H2O -> D_Fructose + Sucrose		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM sodium acetate, 0.02% (v/v) sodium azide
PH: 5
Temperature: 30
uniprot:Q04937 [beta-fructofuranosidase(Enzyme) wildtype]

H2O + Sucrose -> beta_D_Fructose + alpha_D_Glucose		 (( (Vmax * S) ) / (Km + S)) buffer: 0.2 M potassium phosphate, 10 mM MgCl2, 10 mM ATP, 1 mM NADP+, 10 U/ml glucose-6-phosphate dehydrogenase, 10 U/ml hexokinase
PH: 6.5
Temperature: 22
uniprot:P13522 [beta-fructofuranosidase(Enzyme) wildtype]

Sucrose_6_phosphate + H2O -> beta_D_Fructose + alpha_D_Glucose_6_phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.2 M MES, 1 mM NADP+, 10 U/ml glucose-6-phosphate dehydrogenase
PH: 7.1
Temperature: 22
uniprot:Q04937 [beta-fructofuranosidase(Enzyme) wildtype]

Sucrose_6_phosphate + H2O -> alpha_D_Glucose_6_phosphate + beta_D_Fructose		 (( (Vmax * S) ) / (Km + S)) buffer: 0.2 M MES, 1 mM NADP+, 10 U/ml glucose-6-phosphate dehydrogenase
PH: 7.1
Temperature: 22
uniprot:Q7XA49 [beta-fructofuranosidase(Enzyme) wildtype]

H2O + Sucrose -> D_Fructose + D_Glucose		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Hepes, 1 mM Mg-acetate
PH: 7
Temperature: 25
uniprot:P29000 [beta-fructofuranosidase(Enzyme) wildtype]

H2O + Sucrose -> D_Fructose + D_Glucose		 (( (Vmax * A) ) / (Km + A)) buffer: 66.7 mM sodium acetate
PH: 4.9
Temperature: 37
- [beta-fructofuranosidase(Enzyme) wildtype]

H2O + Sucrose -> D_Glucose + D_Fructose		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Hepes-KOH, 2 mM MgCl2, 1 mM EDTA, 15 mM KCl, 0.4 mM NAD, 1 mM ATP, 2 U/ml hexokinase, 2 U/ml glucose 6-phosphate dehydrogenase, 2 U/ml phosphoglucoseisomerase
PH: 7
Temperature: 25
uniprot:Q9ZR47 [beta-fructofuranosidase(Enzyme) wildtype neutral Invertase]

H2O + Sucrose -> D_Fructose + D_Glucose		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Potassium phosphate
PH: 6.8
Temperature: 37
- [beta-fructofuranosidase(Enzyme) wildtype alkaline Invertase]

Sucrose + H2O -> D_Fructose + D_Glucose		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Potassium phosphate
PH: 8
Temperature: 37
uniprot:Q8GP32 [Ca2+]

Sucrose + H2O -> alpha_D_Glucose + beta_D_Fructose		 (( (( (kcat * E) ) * S) ) / (( (Km + S) ) + (( (S ^ 2) ) / Ki))) buffer: 25 mM sodium acetate
PH: 5.4
Temperature: 50
uniprot:Q8GP32 [Ca2+]

Sucrose + H2O -> beta_D_Fructose + alpha_D_Glucose		 (( (( (kcat * E) ) * S) ) / (( (Km + S) ) + (( (S ^ 2) ) / Ki))) buffer: 25 mM sodium acetate
PH: 5.4
Temperature: 20
uniprot:Q9ZR47 [beta-fructofuranosidase(Enzyme) wildtype neutral Invertase]

Sucrose + H2O -> D_Glucose + D_Fructose		 (( (Vmax * A) ) / (Km + A)) buffer: 0-15 mM Tris-HCl
PH: 7.5
Temperature: 37
- [beta-fructofuranosidase(Enzyme) wildtype alkaline Invertase]

Sucrose + H2O -> D_Glucose + D_Fructose		 (( (Vmax * A) ) / (Km + A)) buffer: 0-15 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:P13522 [beta-fructofuranosidase(Enzyme) wildtype]

Sucrose + H2O -> D_Glucose + D_Fructose		 (( (Vmax * S) ) / (Km + S)) buffer: 0.10 M potassium phosphate
PH: 7
Temperature: 37
uniprot:P13522 [beta-fructofuranosidase(Enzyme) wildtype]

Sucrose + H2O -> D_Glucose + D_Fructose		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM MES, 10 mM NaF, 0.2 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase
PH: 7.1
Temperature: 37
uniprot:P13522 [beta-fructofuranosidase(Enzyme) wildtype]

H2O + Sucrose_6_phosphate -> D_Fructose + D_Glucose_6_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM MES, 10 mM NaF, 0.2 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase
PH: 7.1
Temperature: 37

Pathways

pathway id name
BioCyc:META_PWY-621 sucrose degradation III (sucrose invertase)
BioCyc:ARA_PWYQT-4466 superpathway of sucrose and starch metabolism I (non-photosynthetic tissue)
BioCyc:BSUB_SUCUTIL-PWY sucrose degradation I (sucrose phosphotransferase)
BioCyc:ARA_PWY-621 sucrose degradation III (sucrose invertase)
BioCyc:META_SUCUTIL-PWY sucrose degradation I (sucrose phosphotransferase)
BioCyc:YEAST_PWY-621 sucrose degradation III
Plant Reactome:R-OSA-9030769 Growth and developmental processes
Plant Reactome:R-OSA-9031670 Vegetative structure development
Plant Reactome:R-OSA-9623902 Seed development
Plant Reactome:R-OSA-9640032 Root structure development
Plant Reactome:R-OSA-9035605 Regulation of seed size
Plant Reactome:R-OSA-9031669 Reproductive structure development
Plant Reactome:R-OSA-9030654 Primary root development
PlantCyc:ARA_PWY-621 sucrose degradation III (sucrose invertase)
PlantCyc:PLANT_PWY-621 sucrose degradation III (sucrose invertase)
PlantCyc:ARA_PWYQT-4466 superpathway of sucrose and starch metabolism I (non-photosynthetic tissue)
PlantCyc:PLANT_PWYQT-4466 superpathway of sucrose and starch metabolism I (non-photosynthetic tissue)
WikiPathways:WP2623 Sucrose metabolism
PathBank:SMP0002314 Fructose Metabolism
PathBank:SMP0002380 Starch and Sucrose Metabolism