EC: 3.2.1.20

alpha-glucosidase (alpha-D-glucoside glucohydrolase)

enzyme lambda metabolic reaction experiment
- [alpha-glucosidase(Enzyme) wildtype]

p_Nitrophenyl_alpha_D_glucoside + H2O -> alpha_D_Glucose + p_Nitrophenol		 (( (Vmax * S) ) / (Km + S)) buffer: 0.067 M KH2PO4
PH: 7
Temperature: 28
uniprot:A0A222C758 [Ca2+]

p_Nitrophenyl_alpha_D_glucoside + H2O -> p_Nitrophenol + alpha_D_Glucose		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM sodium phosphate, 100 mM glutathione (reduced)
PH: 6.8
Temperature: 25
uniprot:Q9AI65 [1,4-Dithiothreitol]

H2O + Sucrose -> alpha_D_Glucose + beta_D_Fructose		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Tris-HCl, 1 mM MgCl2, 1 mM NADP+, 5 mM ATP, 3 U/ml Glucose 6-phosphate dehydrogenase/Hexokinase
PH: 7.5
Temperature: 25
uniprot:Q9AI65 [1,4-Dithiothreitol]

H2O + Palatinose -> alpha_D_Glucose + D_Fructose		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Tris-HCl, 1 mM MgCl2, 1 mM NADP+, 5 mM ATP, 3 U/ml Glucose 6-phosphate dehydrogenase/Hexokinase
PH: 7.5
Temperature: 25
uniprot:Q9AI65 [1,4-Dithiothreitol]

H2O + Trehalulose -> beta_D_Fructose + alpha_D_Glucose		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Tris-HCl, 1 mM MgCl2, 1 mM NADP+, 5 mM ATP, 3 U/ml Glucose 6-phosphate dehydrogenase/Hexokinase
PH: 7.5
Temperature: 25
uniprot:Q9AI65 [1,4-Dithiothreitol]

p_Nitrophenol_alpha_D_glucopyranoside + H2O -> p_Nitrophenol + alpha_D_Glucose		 (( (Vmax * C) ) / (Km + C)) buffer: 0.1 M Tris-HCl, 1 mM MgCl2, 1 mM NADP+, 5 mM ATP, 3 U/ml Glucose 6-phosphate dehydrogenase/Hexokinase
PH: 7.5
Temperature: 25
uniprot:Q9AI65 [1,4-Dithiothreitol]

H2O + p_Nitrophenol_alpha_D_glucopyranoside -> p_Nitrophenol + alpha_D_Glucose		 (( (Vmax * B) ) / (Km + B)) buffer: 0.1 M Tris-HCl, 1 mM MgCl2, 1 mM NADP+, 5 mM ATP, 3 U/ml Glucose 6-phosphate dehydrogenase/Hexokinase
PH: 7.5
Temperature: 25
uniprot:Q9AI65 [1,4-Dithiothreitol]

H2O + Leucrose -> alpha_D_Glucose + D_Fructose		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Tris-HCl, 1 mM MgCl2, 1 mM NADP+, 5 mM ATP, 3 U/ml Glucose 6-phosphate dehydrogenase/Hexokinase
PH: 7.5
Temperature: 25
uniprot:Q9AI65 [1,4-Dithiothreitol]

H2O + Turanose -> alpha_D_Glucose + D_Fructose		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Tris-HCl, 1 mM MgCl2, 1 mM NADP+, 5 mM ATP, 3 U/ml Glucose 6-phosphate dehydrogenase/Hexokinase
PH: 7.5
Temperature: 25
uniprot:Q9AI65 [1,4-Dithiothreitol]

H2O + Maltulose -> D_Fructose + alpha_D_Glucose		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Tris-HCl, 1 mM MgCl2, 1 mM NADP+, 5 mM ATP, 3 U/ml Glucose 6-phosphate dehydrogenase/Hexokinase
PH: 7.5
Temperature: 25
uniprot:Q9AI65 [1,4-Dithiothreitol]

H2O + Maltose -> 2 alpha_D_Glucose		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Tris-HCl, 1 mM MgCl2, 1 mM NADP+, 5 mM ATP, 3 U/ml Glucose 6-phosphate dehydrogenase/Hexokinase
PH: 7.5
Temperature: 25
uniprot:Q9AI65 [1,4-Dithiothreitol]

p_Nitrophenol_alpha_D_glucopyranoside + H2O -> alpha_D_Glucose + p_Nitrophenol		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Tris-HCl, 1 mM MgCl2, 1 mM NADP+, 5 mM ATP, 3 U/ml Glucose 6-phosphate dehydrogenase/Hexokinase
PH: 7.5
Temperature: 25
uniprot:Q9AI65 [1,4-Dithiothreitol]

alpha,alpha_Trehalose + H2O -> 2 alpha_D_Glucose		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Tris-HCl, 1 mM MgCl2, 1 mM NADP+, 5 mM ATP, 3 U/ml Glucose 6-phosphate dehydrogenase/Hexokinase
PH: 7.5
Temperature: 25
uniprot:Q9AI65 [1,4-Dithiothreitol]

Maltitol + H2O -> alpha_D_Glucose + D_Sorbitol		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Tris-HCl, 1 mM MgCl2, 1 mM NADP+, 5 mM ATP, 3 U/ml Glucose 6-phosphate dehydrogenase/Hexokinase
PH: 7.5
Temperature: 25
- [alpha-glucosidase(Enzyme) wildtype]

alpha,alpha_Trehalose + H2O -> 2 D_Glucose		 (( (Vmax * Substrate) ) / (Km + Substrate)) buffer: 1.0 mM sodium phosphate
PH: 6
Temperature: 55
- [alpha-glucosidase(Enzyme) mutant Arg81Ser]

alpha,alpha_Trehalose + H2O -> 2 D_Glucose		 (( (Vmax * Substrate) ) / (Km + Substrate)) buffer: 1.0 mM sodium phosphate
PH: 6
Temperature: 55
- [alpha-glucosidase(Enzyme) mutant Ala116Val]

alpha,alpha_Trehalose + H2O -> 2 D_Glucose		 (( (Vmax * Substrate) ) / (Km + Substrate)) buffer: 1.0 mM sodium phosphate
PH: 6
Temperature: 55
- [alpha-glucosidase(Enzyme) mutant Met76Asn]

H2O + alpha,alpha_Trehalose -> 2 D_Glucose		 (( (Vmax * Substrate) ) / (Km + Substrate)) buffer: 1.0 mM sodium phosphate
PH: 6
Temperature: 55
- [alpha-glucosidase(Enzyme) mutant Met76Asn/Arg81Ser/Ala116Val/Thr342Asn]

H2O + alpha,alpha_Trehalose -> 2 D_Glucose		 (( (Vmax * Substrate) ) / (Km + Substrate)) buffer: 1.0 mM sodium phosphate
PH: 6
Temperature: 55
- [alpha-glucosidase(Enzyme) mutant Met76Asn/Arg81Ser/Ala116Val/Gly273Pro/Thr342Asn]

alpha,alpha_Trehalose + H2O -> 2 D_Glucose		 (( (Vmax * Substrate) ) / (Km + Substrate)) buffer: 1.0 mM sodium phosphate
PH: 6
Temperature: 55
- [alpha-glucosidase(Enzyme) mutant Gly273Pro]

H2O + alpha,alpha_Trehalose -> 2 D_Glucose		 (( (Vmax * Substrate) ) / (Km + Substrate)) buffer: 1.0 mM sodium phosphate
PH: 6
Temperature: 55
- [alpha-glucosidase(Enzyme) mutant Gly273Pro/Thr342Asn]

alpha,alpha_Trehalose + H2O -> 2 D_Glucose		 (( (Vmax * Substrate) ) / (Km + Substrate)) buffer: 1.0 mM sodium phosphate
PH: 6
Temperature: 55
- [alpha-glucosidase(Enzyme) mutant Thr342Asn]

alpha,alpha_Trehalose + H2O -> 2 D_Glucose		 (( (Vmax * Substrate) ) / (Km + Substrate)) buffer: 1.0 mM sodium phosphate
PH: 6
Temperature: 55
- [alpha-glucosidase(Enzyme) mutant Met76Asn/Arg81Ser/Ala116Val/Gly273Pro]

alpha,alpha_Trehalose + H2O -> 2 D_Glucose		 (( (Vmax * Substrate) ) / (Km + Substrate)) buffer: 1.0 mM sodium phosphate
PH: 6
Temperature: 55
- [alpha-glucosidase(Enzyme) mutant Met76Asn/Arg81Ser/Gly273Pro/Thr342Asn]

H2O + alpha,alpha_Trehalose -> 2 D_Glucose		 (( (Vmax * Substrate) ) / (Km + Substrate)) buffer: 1.0 mM sodium phosphate
PH: 6
Temperature: 55
- [alpha-glucosidase(Enzyme) mutant Arg81Ser/Ala116Val/Gly273Pro/Thr342Asn]

H2O + alpha,alpha_Trehalose -> 2 D_Glucose		 (( (Vmax * Substrate) ) / (Km + Substrate)) buffer: 1.0 mM sodium phosphate
PH: 6
Temperature: 55
- [alpha-glucosidase(Enzyme) mutant Met76Asn/Ala116Val/Gly273Pro/Thr342Asn]

H2O + alpha,alpha_Trehalose -> 2 D_Glucose		 (( (Vmax * Substrate) ) / (Km + Substrate)) buffer: 1.0 mM sodium phosphate
PH: 6
Temperature: 55
- [alpha-glucosidase(Enzyme) mutant Met76Asn/Arg81Ser/Ala116Val/Gly273Pro/Thr342Asn]

Maltose + H2O -> 2 alpha_D_Glucose		 (( (Vmax * Substrate) ) / (Km + Substrate)) buffer: 1.0 mM sodium phosphate
PH: 6
Temperature: 55
- [alpha-glucosidase(Enzyme) mutant Arg81Ser]

H2O + Maltose -> 2 alpha_D_Glucose		 (( (Vmax * Substrate) ) / (Km + Substrate)) buffer: 1.0 mM sodium phosphate
PH: 6
Temperature: 55
- [alpha-glucosidase(Enzyme) mutant Met76Asn]

H2O + Maltose -> 2 alpha_D_Glucose		 (( (Vmax * Substrate) ) / (Km + Substrate)) buffer: 1.0 mM sodium phosphate
PH: 6
Temperature: 55
- [alpha-glucosidase(Enzyme) mutant Met76Asn/Arg81Ser/Ala116Val/ Thr342Asn]

H2O + Maltose -> 2 alpha_D_Glucose		 (( (Vmax * Substrate) ) / (Km + Substrate)) buffer: 1.0 mM sodium phosphate
PH: 6
Temperature: 55
- [alpha-glucosidase(Enzyme) mutant Ala116Val]

Maltose + H2O -> 2 alpha_D_Glucose		 (( (Vmax * Substrate) ) / (Km + Substrate)) buffer: 1.0 mM sodium phosphate
PH: 6
Temperature: 55
- [alpha-glucosidase(Enzyme) mutant Gly273Pro]

Maltose + H2O -> 2 alpha_D_Glucose		 (( (Vmax * Substrate) ) / (Km + Substrate)) buffer: 1.0 mM sodium phosphate
PH: 6
Temperature: 55
- [alpha-glucosidase(Enzyme) wildtype]

H2O + Maltose -> 2 alpha_D_Glucose		 (( (Vmax * Substrate) ) / (Km + Substrate)) buffer: 1.0 mM sodium phosphate
PH: 6
Temperature: 55
- [alpha-glucosidase(Enzyme) mutant Met76Asn/Arg81Ser/Ala116Val/ Thr342Asn]

H2O + Sucrose -> alpha_D_Glucose + beta_D_Fructose		 (( (Vmax * Substrate) ) / (Km + Substrate)) buffer: 1.0 mM sodium phosphate
PH: 6
Temperature: 55
- [alpha-glucosidase(Enzyme) mutant Thr342Asn]

H2O + Maltose -> 2 alpha_D_Glucose		 (( (Vmax * Substrate) ) / (Km + Substrate)) buffer: 1.0 mM sodium phosphate
PH: 6
Temperature: 55
- [alpha-glucosidase(Enzyme) mutant Ala116Val]

Sucrose + H2O -> beta_D_Fructose + alpha_D_Glucose		 (( (Vmax * Substrate) ) / (Km + Substrate)) buffer: 1.0 mM sodium phosphate
PH: 6
Temperature: 55
- [alpha-glucosidase(Enzyme) wildtype]

H2O + Sucrose -> alpha_D_Glucose + beta_D_Fructose		 (( (Vmax * Substrate) ) / (Km + Substrate)) buffer: 1.0 mM sodium phosphate
PH: 6
Temperature: 55
- [alpha-glucosidase(Enzyme) mutant Thr342Asn]

Sucrose + H2O -> alpha_D_Glucose + beta_D_Fructose		 (( (Vmax * Substrate) ) / (Km + Substrate)) buffer: 1.0 mM sodium phosphate
PH: 6
Temperature: 55
- [alpha-glucosidase(Enzyme) mutant Arg81Ser]

H2O + Sucrose -> beta_D_Fructose + alpha_D_Glucose		 (( (Vmax * Substrate) ) / (Km + Substrate)) buffer: 1.0 mM sodium phosphate
PH: 6
Temperature: 55
- [alpha-glucosidase(Enzyme) mutant Met76Asn/Arg81Ser/Ala116Val/Thr342Asn]

H2O + Sucrose -> beta_D_Fructose + alpha_D_Glucose		 (( (Vmax * Substrate) ) / (Km + Substrate)) buffer: 1.0 mM sodium phosphate
PH: 6
Temperature: 55
- [alpha-glucosidase(Enzyme) mutant Met76Asn]

H2O + Sucrose -> beta_D_Fructose + alpha_D_Glucose		 (( (Vmax * Substrate) ) / (Km + Substrate)) buffer: 1.0 mM sodium phosphate
PH: 6
Temperature: 55
- [alpha-glucosidase(Enzyme) mutant Gly273Pro]

H2O + Sucrose -> beta_D_Fructose + alpha_D_Glucose		 (( (Vmax * Substrate) ) / (Km + Substrate)) buffer: 1.0 mM sodium phosphate
PH: 6
Temperature: 55
- [alpha-glucosidase(Enzyme) mutant Met76Asn/Arg81Ser/Ala116Val/Thr342Asn]

H2O + Isomaltose -> 2 alpha_D_Glucose		 (( (Vmax * Substrate) ) / (Km + Substrate)) buffer: 1.0 mM sodium phosphate
PH: 6
Temperature: 55
- [alpha-glucosidase(Enzyme) wildtype]

H2O + Isomaltose -> 2 alpha_D_Glucose		 (( (Vmax * Substrate) ) / (Km + Substrate)) buffer: 1.0 mM sodium phosphate
PH: 6
Temperature: 55
- [alpha-glucosidase(Enzyme) mutant Gly273Pro]

Isomaltose + H2O -> 2 alpha_D_Glucose		 (( (Vmax * Substrate) ) / (Km + Substrate)) buffer: 1.0 mM sodium phosphate
PH: 6
Temperature: 55
- [alpha-glucosidase(Enzyme) mutant Met76Asn/Ala116Val/Gly273Pro/Thr342Asn]

H2O + Isomaltose -> 2 alpha_D_Glucose		 (( (Vmax * Substrate) ) / (Km + Substrate)) buffer: 1.0 mM sodium phosphate
PH: 6
Temperature: 55
- [alpha-glucosidase(Enzyme) mutant Thr342Asn]

H2O + Isomaltose -> 2 alpha_D_Glucose		 (( (Vmax * Substrate) ) / (Km + Substrate)) buffer: 1.0 mM sodium phosphate
PH: 6
Temperature: 55
- [alpha-glucosidase(Enzyme) mutant Met76Asn]

H2O + Isomaltose -> 2 alpha_D_Glucose		 (( (Vmax * Substrate) ) / (Km + Substrate)) buffer: 1.0 mM sodium phosphate
PH: 6
Temperature: 55
- [alpha-glucosidase(Enzyme) mutant Arg81Ser/Ala116Val/Gly273Pro/Thr342Asn]

Isomaltose + H2O -> 2 alpha_D_Glucose		 (( (Vmax * Substrate) ) / (Km + Substrate)) buffer: 1.0 mM sodium phosphate
PH: 6
Temperature: 55
- [alpha-glucosidase(Enzyme) mutant Met76Asn/Arg81Ser/Gly273Pro/Thr342Asn]

H2O + Isomaltose -> 2 alpha_D_Glucose		 (( (Vmax * Substrate) ) / (Km + Substrate)) buffer: 1.0 mM sodium phosphate
PH: 6
Temperature: 55
- [alpha-glucosidase(Enzyme) mutant Gly273Pro/Thr342Asn]

Isomaltose + H2O -> 2 alpha_D_Glucose		 (( (Vmax * Substrate) ) / (Km + Substrate)) buffer: 1.0 mM sodium phosphate
PH: 6
Temperature: 55
- [alpha-glucosidase(Enzyme) mutant Ala116Val]

H2O + Isomaltose -> 2 alpha_D_Glucose		 (( (Vmax * Substrate) ) / (Km + Substrate)) buffer: 1.0 mM sodium phosphate
PH: 6
Temperature: 55
- [alpha-glucosidase(Enzyme) mutant Arg81Ser]

H2O + Isomaltose -> 2 alpha_D_Glucose		 (( (Vmax * Substrate) ) / (Km + Substrate)) buffer: 1.0 mM sodium phosphate
PH: 6
Temperature: 55
- [alpha-glucosidase(Enzyme) mutant Met76Asn/Arg81Ser/Ala116Val/Gly273Pro]

Isomaltose + H2O -> 2 alpha_D_Glucose		 (( (Vmax * Substrate) ) / (Km + Substrate)) buffer: 1.0 mM sodium phosphate
PH: 6
Temperature: 55
- [alpha-glucosidase(Enzyme) mutant Met76Asn/Arg81Ser/Ala116Val/Gly273Pro/Thr342Asn]

Isomaltose + H2O -> 2 alpha_D_Glucose		 (( (Vmax * Substrate) ) / (Km + Substrate)) buffer: 1.0 mM sodium phosphate
PH: 6
Temperature: 55
uniprot:Q17058 [alpha-glucosidase(Enzyme) wildtype alpha-Glucosidase III]

H2O + Maltotriose -> Maltose + alpha_D_Glucose		 (( (Vmax * A) ) / (Km + A)) buffer: 0.04 M Sodium acetate
PH: 5.5
Temperature: 35
uniprot:Q17058 [alpha-glucosidase(Enzyme) wildtype alpha-Glucosidase III]

Maltose + H2O -> 2 alpha_D_Glucose		 (( (Vmax * A) ) / (Km + A)) buffer: 0.04 M Sodium acetate
PH: 5.5
Temperature: 35
uniprot:Q17058 [alpha-glucosidase(Enzyme) wildtype alpha-Glucosidase III]

Maltotetraose + H2O -> Maltotriose + alpha_D_Glucose		 (( (Vmax * A) ) / (Km + A)) buffer: 0.04 M Sodium acetate
PH: 5.5
Temperature: 35
uniprot:Q17058 [alpha-glucosidase(Enzyme) wildtype alpha-Glucosidase III]

H2O + Maltopentaose -> alpha_D_Glucose + Maltotetraose		 (( (Vmax * A) ) / (Km + A)) buffer: 0.04 M Sodium acetate
PH: 5.5
Temperature: 35
uniprot:Q17058 [alpha-glucosidase(Enzyme) wildtype alpha-Glucosidase III]

Sucrose + H2O -> beta_D_Fructose + alpha_D_Glucose		 (( (Vmax * A) ) / (Km + A)) buffer: 0.04 M Sodium acetate
PH: 5.5
Temperature: 35
uniprot:Q17058 [alpha-glucosidase(Enzyme) wildtype alpha-Glucosidase III]

Isomaltose + H2O -> 2 alpha_D_Glucose		 (( (Vmax * A) ) / (Km + A)) buffer: 0.04 M Sodium acetate
PH: 5.5
Temperature: 35
uniprot:Q17058 [alpha-glucosidase(Enzyme) wildtype alpha-Glucosidase III]

H2O + p_Nitrophenyl_alpha_D_glucoside -> p_Nitrophenol + alpha_D_Glucose		 (( (Vmax * A) ) / (Km + A)) buffer: 0.04 M Sodium acetate
PH: 5.5
Temperature: 35
uniprot:Q17058 [alpha-glucosidase(Enzyme) wildtype alpha-Glucosidase III]

Phenyl_alpha_D_maltoside + H2O -> Phenol + Maltose		 (( (Vmax * A) ) / (Km + A)) buffer: 0.04 M Sodium acetate
PH: 5.5
Temperature: 35
uniprot:Q17058 [alpha-glucosidase(Enzyme) wildtype alpha-Glucosidase III]

H2O + Phenyl_alpha_D_glucoside -> alpha_D_Glucose + Phenol		 (( (Vmax * A) ) / (Km + A)) buffer: 0.04 M Sodium acetate
PH: 5.5
Temperature: 35
uniprot:Q17058 [alpha-glucosidase(Enzyme) wildtype alpha-Glucosidase III]

H2O + Turanose -> D_Fructose + alpha_D_Glucose		 (( (Vmax * A) ) / (Km + A)) buffer: 0.04 M Sodium acetate
PH: 5.5
Temperature: 35
uniprot:Q17058 [alpha-glucosidase(Enzyme) wildtype alpha-Glucosidase III]

Maltohexaose + H2O -> alpha_D_Glucose + Maltopentaose		 (( (Vmax * A) ) / (Km + A)) buffer: 0.04 M Sodium acetate
PH: 5.5
Temperature: 35
uniprot:Q17058 [alpha-glucosidase(Enzyme) wildtype alpha-Glucosidase III]

Nigerose + H2O -> 2 alpha_D_Glucose		 (( (Vmax * A) ) / (Km + A)) buffer: 0.04 M Sodium acetate
PH: 5.5
Temperature: 35
uniprot:Q17058 [alpha-glucosidase(Enzyme) wildtype alpha-Glucosidase III]

H2O + Kojibiose -> 2 alpha_D_Glucose		 (( (Vmax * A) ) / (Km + A)) buffer: 0.04 M Sodium acetate
PH: 5.5
Temperature: 35
- [alpha-glucosidase(Enzyme) wildtype]

Maltose + H2O -> 2 alpha_D_Glucose		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Sodium acetate
PH: 5.5
Temperature: 50
- [alpha-glucosidase(Enzyme) wildtype]

H2O + Isomaltose -> 2 alpha_D_Glucose		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Sodium acetate
PH: 5.5
Temperature: 50
- [alpha-glucosidase(Enzyme) wildtype]

H2O + 2_O_beta_D_Glucopyranosyl_beta_D_glucopyranose -> 2 beta_D_Glucose		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Sodium acetate
PH: 5.5
Temperature: 50
- [alpha-glucosidase(Enzyme) wildtype]

H2O + Starch -> alpha_D_Glucose + Starch		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Sodium acetate
PH: 5.5
Temperature: 50
- [alpha-glucosidase(Enzyme) wildtype]

H2O + Nigerose -> 2 alpha_D_Glucose		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Sodium acetate
PH: 5.5
Temperature: 50
uniprot:Q9C1S7 [alpha-glucosidase(Enzyme) wildtype]

Maltotriose + H2O -> Maltose + alpha_D_Glucose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Acetate
PH: 5.5
Temperature: 45
uniprot:Q9C1S7 [alpha-glucosidase(Enzyme) wildtype]

H2O + Isomaltose -> 2 alpha_D_Glucose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Acetate
PH: 5.5
Temperature: 45
uniprot:Q9C1S7 [alpha-glucosidase(Enzyme) wildtype]

p_Nitrophenyl_alpha_D_glucoside + H2O -> p_Nitrophenol + alpha_D_Glucose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Acetate
PH: 5.5
Temperature: 45
uniprot:Q9C1S7 [alpha-glucosidase(Enzyme) wildtype]

H2O + Maltotetraose -> alpha_D_Glucose + Maltotriose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Acetate
PH: 5.5
Temperature: 45
uniprot:Q9C1S7 [alpha-glucosidase(Enzyme) wildtype]

Maltopentaose + H2O -> alpha_D_Glucose + Maltotetraose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Acetate
PH: 5.5
Temperature: 45
uniprot:Q9C1S7 [alpha-glucosidase(Enzyme) wildtype]

alpha,alpha_Trehalose + H2O -> 2 alpha_D_Glucose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Acetate
PH: 5.5
Temperature: 45
uniprot:Q9C1S7 [alpha-glucosidase(Enzyme) wildtype]

H2O + Nigerose -> 2 alpha_D_Glucose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Acetate
PH: 5.5
Temperature: 45
uniprot:Q9C1S7 [alpha-glucosidase(Enzyme) wildtype]

Kojibiose + H2O -> 2 alpha_D_Glucose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Acetate
PH: 5.5
Temperature: 45
uniprot:Q9C1S7 [alpha-glucosidase(Enzyme) wildtype]

H2O + Maltose -> 2 alpha_D_Glucose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Acetate
PH: 5.5
Temperature: 45
uniprot:Q876Z7 [alpha-glucosidase(Enzyme) wildtype]

H2O + Maltoheptaose -> alpha_D_Glucose + Maltohexaose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Sodium acetate
PH: 5
Temperature: 37
uniprot:Q876Z7 [alpha-glucosidase(Enzyme) wildtype]

Maltohexaose + H2O -> Maltopentaose + alpha_D_Glucose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Sodium acetate
PH: 5
Temperature: 37
uniprot:Q876Z7 [alpha-glucosidase(Enzyme) wildtype]

Maltopentaose + H2O -> Maltotetraose + alpha_D_Glucose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Sodium acetate
PH: 5
Temperature: 37
- [Ca2+]

H2O + Maltose -> 2 alpha_D_Glucose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM BTP
PH: 7
Temperature: 70
uniprot:Q876Z7 [alpha-glucosidase(Enzyme) wildtype]

Maltotriose + H2O -> alpha_D_Glucose + Maltose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Sodium acetate
PH: 5
Temperature: 37
uniprot:Q876Z7 [alpha-glucosidase(Enzyme) wildtype]

H2O + Maltose -> 2 alpha_D_Glucose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Sodium acetate
PH: 5
Temperature: 37
uniprot:Q876Z7 [alpha-glucosidase(Enzyme) wildtype]

H2O + Maltotetraose -> Maltotriose + alpha_D_Glucose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Sodium acetate
PH: 5
Temperature: 37
uniprot:Q72LF2 [Ca2+]

alpha,alpha_Trehalose + H2O -> 2 D_Glucose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM BTP
PH: 7
Temperature: 70
- [alpha-glucosidase(Enzyme) wildtype]

Maltose + H2O -> 2 alpha_D_Glucose		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Sodium phosphate
PH: 5.5
Temperature: 108
- [alpha-glucosidase(Enzyme) wildtype]

alpha_Methyl_D_glucopyranoside + H2O -> alpha_D_Glucose + Methanol		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Sodium phosphate
PH: 5.5
Temperature: 108
- [alpha-glucosidase(Enzyme) wildtype]

p_Nitrophenyl_alpha_D_glucoside + H2O -> p_Nitrophenol + alpha_D_Glucose		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Sodium phosphate
PH: 5.5
Temperature: 108
uniprot:Q72LF2 [Ca2+]

H2O + Maltose -> 2 alpha_D_Glucose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM BTP
PH: 7
Temperature: 70
uniprot:Q72LF2 [Ca2+]

H2O + Maltose -> 2 alpha_D_Glucose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM BTP
PH: 7
Temperature: 70
- [Ca2+]

H2O + Maltose -> 2 alpha_D_Glucose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM BTP
PH: 7
Temperature: 70
uniprot:Q72LF2 [Ca2+]

alpha,alpha_Trehalose + H2O -> 2 D_Glucose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM BTP
PH: 7
Temperature: 70
uniprot:Q72LF2 [Ca2+]

Isomaltose + H2O -> 2 alpha_D_Glucose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM BTP
PH: 7
Temperature: 70
uniprot:Q72LF2 [Ca2+]

Isomaltose + H2O -> 2 alpha_D_Glucose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM BTP
PH: 7
Temperature: 70
- [Ca2+]

H2O + alpha,alpha_Trehalose -> 2 D_Glucose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM BTP
PH: 7
Temperature: 70
- [Ca2+]

H2O + Isomaltose -> 2 alpha_D_Glucose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM BTP
PH: 7
Temperature: 70
- [Ca2+]

Isomaltose + H2O -> 2 alpha_D_Glucose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM BTP
PH: 7
Temperature: 70
- [alpha-glucosidase(Enzyme) wildtype]

H2O + Isomaltose -> 2 alpha_D_Glucose		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Sodium phosphate
PH: 5.5
Temperature: 108
uniprot:Q6P7A9 [alpha-glucosidase(Enzyme) wildtype]

H2O + p_Nitrophenyl_alpha_D_glucoside -> alpha_D_Glucose + p_Nitrophenol		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M sodium citrate/citric acid
PH: 4.5
Temperature: 37
uniprot:Q6P7A9 [alpha-glucosidase(Enzyme) wildtype]

p_Nitrophenyl_alpha_D_glucoside + H2O -> p_Nitrophenol + alpha_D_Glucose		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M sodium citrate/citric acid
PH: 4.5
Temperature: 37
uniprot:Q6P7A9 [alpha-glucosidase(Enzyme) wildtype]

H2O + p_Nitrophenyl_alpha_D_glucoside -> p_Nitrophenol + alpha_D_Glucose		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M sodium citrate/citric acid
PH: 4.5
Temperature: 37
uniprot:Q6P7A9 [alpha-glucosidase(Enzyme) wildtype]

H2O + p_Nitrophenyl_alpha_D_glucoside -> p_Nitrophenol + alpha_D_Glucose		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M sodium citrate/citric acid
PH: 4.5
Temperature: 37
uniprot:Q6P7A9 [alpha-glucosidase(Enzyme) wildtype]

H2O + p_Nitrophenyl_alpha_D_glucoside -> alpha_D_Glucose + p_Nitrophenol		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M sodium citrate/citric acid
PH: 4.5
Temperature: 37
uniprot:Q6P7A9 [alpha-glucosidase(Enzyme) wildtype]

p_Nitrophenyl_alpha_D_glucoside + H2O -> p_Nitrophenol + alpha_D_Glucose		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M sodium citrate/citric acid
PH: 4.5
Temperature: 37
uniprot:Q6P7A9 [alpha-glucosidase(Enzyme) wildtype]

p_Nitrophenyl_alpha_D_glucoside + H2O -> alpha_D_Glucose + p_Nitrophenol		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M sodium citrate/citric acid
PH: 4.5
Temperature: 37
uniprot:Q6P7A9 [alpha-glucosidase(Enzyme) wildtype]

p_Nitrophenyl_alpha_D_glucoside + H2O -> p_Nitrophenol + alpha_D_Glucose		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M sodium citrate/citric acid
PH: 4.5
Temperature: 37
uniprot:Q6P7A9 [alpha-glucosidase(Enzyme) wildtype]

H2O + p_Nitrophenyl_alpha_D_glucoside -> alpha_D_Glucose + p_Nitrophenol		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M sodium citrate/citric acid
PH: 4.5
Temperature: 37
uniprot:Q6P7A9 [alpha-glucosidase(Enzyme) wildtype]

p_Nitrophenyl_alpha_D_glucoside + H2O -> p_Nitrophenol + alpha_D_Glucose		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M sodium citrate/citric acid
PH: 4.5
Temperature: 37
uniprot:P23739 [alpha-glucosidase(Enzyme) wildtype isoenzyme II]

Maltose + H2O -> 2 alpha_D_Glucose		 (( (Vmax * S) ) / (Km + S)) buffer: 68 mM Acetate, 1 mM EDTA
PH: 6
Temperature: 37
uniprot:Q6P7A9 [alpha-glucosidase(Enzyme) wildtype isoenzyme I]

H2O + Maltose -> 2 alpha_D_Glucose		 (( (Vmax * S) ) / (Km + S)) buffer: 68 mM Acetate, 1 mM EDTA
PH: 6
Temperature: 37
uniprot:P23739 [alpha-glucosidase(Enzyme) wildtype isoenzyme II]

Maltose + 2 L_Ascorbate -> 2 H2O + 2 2_O_alpha_D_Glucopyranosyl_L_ascorbic_acid		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Acetate, 13 mM Thiourea
PH: 5.3
Temperature: 50
uniprot:Q6P7A9 [alpha-glucosidase(Enzyme) wildtype isoenzyme I]

Maltose + 2 L_Ascorbate -> 2 2_O_alpha_D_Glucopyranosyl_L_ascorbic_acid + 2 H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Acetate, 13 mM Thiourea
PH: 5.3
Temperature: 50
uniprot:P23739 [alpha-glucosidase(Enzyme) wildtype isoenzyme II]

2_O_alpha_D_Glucopyranosyl_L_ascorbic_acid + H2O -> alpha_D_Glucose + L_Ascorbate		 (( (Vmax * S) ) / (Km + S)) buffer: 68 mM Acetate, 1 mM EDTA
PH: 6
Temperature: 37
uniprot:Q6P7A9 [alpha-glucosidase(Enzyme) wildtype isoenzyme I]

2_O_alpha_D_Glucopyranosyl_L_ascorbic_acid + H2O -> L_Ascorbate + alpha_D_Glucose		 (( (Vmax * S) ) / (Km + S)) buffer: 68 mM Acetate, 1 mM EDTA
PH: 6
Temperature: 37

Pathways

pathway id name
BioCyc:META_GLYCOCAT-PWY glycogen degradation I
BioCyc:ECO_GLYCOCAT-PWY glycogen degradation I
BioCyc:HUMAN_PWY-5941 glycogenolysis
BioCyc:ECOL316407_GLYCOCAT-PWY glycogen degradation I
PathBank:SMP0000958 Starch and Sucrose Metabolism
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-70221 Glycogen breakdown (glycogenolysis)
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-70221 Glycogen breakdown (glycogenolysis)
Reactome:R-HSA-8935690 Digestion
Reactome:R-RNO-8982491 Glycogen metabolism
Reactome:R-SPO-8935690 Digestion
Reactome:R-BTA-71387 Carbohydrate metabolism
Reactome:R-HSA-189085 Digestion of dietary carbohydrate
Reactome:R-MMU-71387 Carbohydrate metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-70221 Glycogen breakdown (glycogenolysis)
Reactome:R-SPO-189085 Digestion of dietary carbohydrate
Reactome:R-BTA-8982491 Glycogen metabolism
Reactome:R-HSA-8963743 Digestion and absorption
Reactome:R-MMU-8982491 Glycogen metabolism
Reactome:R-RNO-71387 Carbohydrate metabolism
Reactome:R-SPO-8963743 Digestion and absorption
PathBank:SMP0000552 Glycogen Synthetase Deficiency
PathBank:SMP0000556 Mucopolysaccharidosis VII. Sly Syndrome
PathBank:SMP0087225 Galactose Metabolism
PathBank:SMP0000043 Galactose Metabolism
PathBank:SMP0000182 Galactosemia
PathBank:SMP0000555 Glycogenosis, Type VI. Hers Disease
PathBank:SMP0000553 Glycogenosis, Type III. Cori Disease, Debrancher Glycogenosis
PathBank:SMP0000557 Sucrase-Isomaltase Deficiency
PathBank:SMP0087252 Starch and Sucrose Metabolism
PathBank:SMP0087320 Galactose Metabolism
PathBank:SMP0120699 Galactosemia
PathBank:SMP0000058 Starch and Sucrose Metabolism
PathBank:SMP0000554 Glycogenosis, Type IV. Amylopectinosis, Anderson Disease