EC: 3.2.1.169

protein O-GlcNAcase ([protein]-3-O-(N-acetyl-D-glucosaminyl)-L-serine/threonine N-acetylglucosaminyl hydrolase)

enzyme lambda metabolic reaction experiment
uniprot:Q0TR53 [protein O-GlcNAcase(Enzyme) mutant D297N]

H2O + 4_Methylumbelliferyl_beta_N_acetylglucosaminide -> N_Acetyl_beta_D_glucosamine + 4_Methylumbelliferone		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM citric acid, 125 mM NaH2PO4, 0.1 mg/ml BSA
PH: 5.5
Temperature: 20
uniprot:Q0TR53 [protein O-GlcNAcase(Enzyme) wildtype]

4_Methylumbelliferyl_beta_N_acetylglucosaminide + H2O -> 4_Methylumbelliferone + N_Acetyl_beta_D_glucosamine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM citric acid, 125 mM NaH2PO4, 0.1 mg/ml BSA
PH: 5.5
Temperature: 20
uniprot:Q0TR53 [protein O-GlcNAcase(Enzyme) mutant Y335F]

H2O + 4_Methylumbelliferyl_beta_N_acetylglucosaminide -> N_Acetyl_beta_D_glucosamine + 4_Methylumbelliferone		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM citric acid, 125 mM NaH2PO4, 0.1 mg/ml BSA
PH: 5.5
Temperature: 20
uniprot:Q0TR53 [protein O-GlcNAcase(Enzyme) mutant D298N]

H2O + 4_Methylumbelliferyl_beta_N_acetylglucosaminide -> 4_Methylumbelliferone + N_Acetyl_beta_D_glucosamine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM citric acid, 125 mM NaH2PO4, 0.1 mg/ml BSA
PH: 5.5
Temperature: 20
uniprot:Q0TR53 [protein O-GlcNAcase(Enzyme) mutant N390A]

H2O + 4_Methylumbelliferyl_beta_N_acetylglucosaminide -> 4_Methylumbelliferone + N_Acetyl_beta_D_glucosamine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM citric acid, 125 mM NaH2PO4, 0.1 mg/ml BSA
PH: 5.5
Temperature: 20
uniprot:Q0TR53 [protein O-GlcNAcase(Enzyme) mutant D401A]

H2O + 4_Methylumbelliferyl_beta_N_acetylglucosaminide -> N_Acetyl_beta_D_glucosamine + 4_Methylumbelliferone		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM citric acid, 125 mM NaH2PO4, 0.1 mg/ml BSA
PH: 5.5
Temperature: 20
uniprot:Q0TR53 [protein O-GlcNAcase(Enzyme) mutant N396A]

H2O + 4_Methylumbelliferyl_beta_N_acetylglucosaminide -> N_Acetyl_beta_D_glucosamine + 4_Methylumbelliferone		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM citric acid, 125 mM NaH2PO4, 0.1 mg/ml BSA
PH: 5.5
Temperature: 20
uniprot:Q0TR53 [protein O-GlcNAcase(Enzyme) mutant W490A]

4_Methylumbelliferyl_beta_N_acetylglucosaminide + H2O -> N_Acetyl_beta_D_glucosamine + 4_Methylumbelliferone		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM citric acid, 125 mM NaH2PO4, 0.1 mg/ml BSA
PH: 5.5
Temperature: 20

Pathways

pathway id name
BioCyc:HUMAN_PWY-7437 protein O-[N-acetyl]-glucosylation
BioCyc:META_PWY-7437 protein O-[N-acetyl]-glucosylation