EC: 3.2.1.1

alpha-amylase (4-alpha-D-glucan glucanohydrolase)

enzyme lambda metabolic reaction experiment
uniprot:P00691 [alpha-amylase(Enzyme) wildtype]

H2O + o_Nitrophenyl_alpha_maltoside -> alpha_Maltose + o_Nitrophenol		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.02 M acetate
PH: 5.3
Temperature: 17
uniprot:Q7X9T1 [alpha-amylase(Enzyme) wildtype VAAmy1]

4,6_O_Benzylidene_4_nitrophenyl_alpha_D_maltoheptaoside + H2O -> p_Nitrophenol + 4,6_O_Benzylidene_alpha_D_maltoheptaoside		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM sodium acetate, 10 mM CaCl2, 0.1 mg/ml BSA
PH: 5.3
Temperature: 37
uniprot:Q7X9T1 [alpha-amylase(Enzyme) wildtype VAAmy2]

H2O + 4,6_O_Benzylidene_4_nitrophenyl_alpha_D_maltoheptaoside -> p_Nitrophenol + 4,6_O_Benzylidene_alpha_D_maltoheptaoside		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM sodium acetate, 10 mM CaCl2, 0.1 mg/ml BSA
PH: 5.2
Temperature: 37
uniprot:Q7X9T1 [alpha-amylase(Enzyme) wildtype VAAmy1]

m_n_n+m_m_n_n+m_n_m_n+m_n_m_n+m_Amylose + H2O -> m_n_n+m_m_n_n+m_n_m_n+m_n_m_n+m_Amylose + m_n_n+m_m_n_n+m_n_m_n+m_n_m_n+m_Amylose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM sodium acetate, 5 mM CaCl2, 0.05 mg/ml BSA
PH: 5.3
Temperature: 37
uniprot:Q7X9T1 [alpha-amylase(Enzyme) wildtype VAAmy2]

m_n_n+m_m_n_n+m_n_m_n+m_n_m_n+m_Amylose + H2O -> m_n_n+m_m_n_n+m_n_m_n+m_n_m_n+m_Amylose + m_n_n+m_m_n_n+m_n_m_n+m_n_m_n+m_Amylose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM sodium acetate, 5 mM CaCl2, 0.05 mg/ml BSA
PH: 5.2
Temperature: 37
uniprot:P04746 [Acarbose]

Maltotriose + H2O -> alpha_D_Glucose + Maltose		 (( (kcat * S) ) / (Km + S)) buffer: 20 mM sodium phosphate, 10 mM chloride
PH: 7
Temperature: 30
uniprot:P04746 [Acarbose]

Maltotriose + H2O -> Maltose + alpha_D_Glucose		 (( (kcat * S) ) / (Km + S)) buffer: 20 mM sodium phosphate, 300 mM chloride
PH: 7
Temperature: 30
uniprot:F9W2W3 [Ca2+]

Starch + H2O -> Starch + Dextrin		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM sodium acetate
PH: 5.5
Temperature: 36
uniprot:F9W2W3 [alpha-amylase(Enzyme) wildtype]

n_m_n+m_n_m_n+m_n_m_n+m_Amylopectin + H2O -> n_m_n+m_n_m_n+m_n_m_n+m_Amylopectin + n_m_n+m_n_m_n+m_n_m_n+m_Amylopectin		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM sodium acetate
PH: 5.5
Temperature: 36
uniprot:Q48502 [alpha-amylase(Enzyme) wildtype]

H2O + 4,6_O_Benzylidene_4_nitrophenyl_alpha_D_maltoheptaoside -> p_Nitrophenol + 4,6_O_Benzylidene_alpha_D_maltoheptaoside		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: Randox assay amylase kit
PH: 5
Temperature: 63
uniprot:O06915 [alpha-amylase(Enzyme) wildtype]

H2O + 4,6_O_Benzylidene_4_nitrophenyl_alpha_D_maltoheptaoside -> 4,6_O_Benzylidene_alpha_D_maltoheptaoside + p_Nitrophenol		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: Randox assay amylase kit
PH: 5
Temperature: 63
uniprot:O06915 [alpha-amylase(Enzyme) wildtype]

H2O + Starch -> Starch + Dextrin		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.1 M citratephosphate
PH: 5
Temperature: 63
uniprot:O06915 [alpha-amylase(Enzyme) wildtype]

Starch + H2O -> Starch + Dextrin		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.1 M citratephosphate
PH: 5
Temperature: 63
uniprot:O06915 [alpha-amylase(Enzyme) wildtype]

H2O + n_m_n+m_n_m_n+m_n_m_n+m_Amylopectin -> n_m_n+m_n_m_n+m_n_m_n+m_Amylopectin + n_m_n+m_n_m_n+m_n_m_n+m_Amylopectin		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.1 M citratephosphate
PH: 5
Temperature: 63
uniprot:O06915 [alpha-amylase(Enzyme) wildtype]

m_n_n+m_m_n_n+m_n_m_n+m_n_m_n+m_Amylose + H2O -> m_n_n+m_m_n_n+m_n_m_n+m_n_m_n+m_Amylose + m_n_n+m_m_n_n+m_n_m_n+m_n_m_n+m_Amylose		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.1 M citratephosphate
PH: 5
Temperature: 63
uniprot:Q48502 [alpha-amylase(Enzyme) wildtype]

Starch + H2O -> Dextrin + Starch		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.1 M citratephosphate
PH: 5
Temperature: 63
uniprot:Q48502 [alpha-amylase(Enzyme) wildtype]

H2O + Starch -> Starch + Dextrin		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.1 M citratephosphate
PH: 5
Temperature: 63
uniprot:Q48502 [alpha-amylase(Enzyme) wildtype]

H2O + n_m_n+m_n_m_n+m_n_m_n+m_Amylopectin -> n_m_n+m_n_m_n+m_n_m_n+m_Amylopectin + n_m_n+m_n_m_n+m_n_m_n+m_Amylopectin		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.1 M citratephosphate
PH: 5
Temperature: 63
uniprot:Q48502 [alpha-amylase(Enzyme) wildtype]

H2O + m_n_n+m_m_n_n+m_n_m_n+m_n_m_n+m_Amylose -> m_n_n+m_m_n_n+m_n_m_n+m_n_m_n+m_Amylose + m_n_n+m_m_n_n+m_n_m_n+m_n_m_n+m_Amylose		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.1 M citratephosphate
PH: 5
Temperature: 63
uniprot:O06915 [Acarbose]

m_n_n+m_n_m_n+m_m_n_n+m_m_n_n+m_m_n_n+m_m_n_n+m_Amylose + H2O -> m_n_n+m_n_m_n+m_m_n_n+m_m_n_n+m_m_n_n+m_m_n_n+m_Amylose + m_n_n+m_n_m_n+m_m_n_n+m_m_n_n+m_m_n_n+m_m_n_n+m_Amylose		 (( (( (kcat * E) ) * S) ) / (Km + (S * (1 + (I / L1i))))) buffer: 20 mM sodium acetate, 1 mM CaCl2, 1 mM sodium azide
PH: 5
Temperature: 40
uniprot:Q9XCV8 [Acarbose]

H2O + m_n_n+m_n_m_n+m_m_n_n+m_m_n_n+m_m_n_n+m_m_n_n+m_Amylose -> m_n_n+m_n_m_n+m_m_n_n+m_m_n_n+m_m_n_n+m_m_n_n+m_Amylose + m_n_n+m_n_m_n+m_m_n_n+m_m_n_n+m_m_n_n+m_m_n_n+m_Amylose		 (( (( (kcat * E) ) * S) ) / (Km + (S * (1 + (I / L1i))))) buffer: 20 mM sodium acetate, 1 mM CaCl2, 1 mM sodium azide
PH: 5
Temperature: 40
- [Acarbose]

m_n_n+m_n_m_n+m_m_n_n+m_m_n_n+m_m_n_n+m_m_n_n+m_Amylose + H2O -> m_n_n+m_n_m_n+m_m_n_n+m_m_n_n+m_m_n_n+m_m_n_n+m_Amylose + m_n_n+m_n_m_n+m_m_n_n+m_m_n_n+m_m_n_n+m_m_n_n+m_Amylose		 (( (( (kcat * E) ) * S) ) / (( (Km * (1 + (I / K1i))) ) + (S * (1 + (I / L1i))))) buffer: 20 mM sodium acetate, 1 mM CaCl2, 1 mM sodium azide
PH: 5
Temperature: 40
uniprot:Q9XCV8 [alpha-amylase(Enzyme) wildtype]

m_n_n+m_n_m_n+m_m_n_n+m_m_n_n+m_m_n_n+m_m_n_n+m_Amylose + H2O -> m_n_n+m_n_m_n+m_m_n_n+m_m_n_n+m_m_n_n+m_m_n_n+m_Amylose + m_n_n+m_n_m_n+m_m_n_n+m_m_n_n+m_m_n_n+m_m_n_n+m_Amylose		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: *
PH: 7
Temperature: 36
uniprot:O06915 [alpha-amylase(Enzyme) wildtype]

H2O + m_n_n+m_n_m_n+m_m_n_n+m_m_n_n+m_m_n_n+m_m_n_n+m_Amylose -> m_n_n+m_n_m_n+m_m_n_n+m_m_n_n+m_m_n_n+m_m_n_n+m_Amylose + m_n_n+m_n_m_n+m_m_n_n+m_m_n_n+m_m_n_n+m_m_n_n+m_Amylose		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: *
PH: 7
Temperature: 36
- [alpha-amylase(Enzyme) wildtype]

m_n_n+m_n_m_n+m_m_n_n+m_m_n_n+m_m_n_n+m_m_n_n+m_Amylose + H2O -> m_n_n+m_n_m_n+m_m_n_n+m_m_n_n+m_m_n_n+m_m_n_n+m_Amylose + m_n_n+m_n_m_n+m_m_n_n+m_m_n_n+m_m_n_n+m_m_n_n+m_Amylose		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: *
PH: 7
Temperature: 36

Pathways

pathway id name
BioCyc:META_PWY-6737 starch degradation V
BioCyc:META_PWY-7900 glycogen biosynthesis III (from α-maltose 1-phosphate)
BioCyc:META_PWY-2622 trehalose biosynthesis IV
BioCyc:MTBH37RV_PWY-2622 trehalose biosynthesis IV
BioCyc:MTBCDC1551_PWY-2661 trehalose biosynthesis V
BioCyc:BTHE_PWY-6737 starch degradation V
BioCyc:MOUSE_PWY-842 starch degradation
BioCyc:MTBH37RV_PWY-7900 glycogen biosynthesis III (from α-maltose 1-phosphate)
BioCyc:MTBCDC1551_PWY-6737 starch degradation V
BioCyc:BTHE_PWY-2661 trehalose biosynthesis V
Reactome:R-MTU-870392 Mycobacterium tuberculosis biological processes
Reactome:R-MTU-868688 Trehalose biosynthesis
PathBank:SMP0120838 Glycogenosis, Type VI. Hers Disease
PathBank:SMP0120837 Glycogenosis, Type IV. Amylopectinosis, Anderson Disease
PathBank:SMP0087345 Starch and Sucrose Metabolism
PathBank:SMP0120836 Glycogenosis, Type III. Cori Disease, Debrancher Glycogenosis
PathBank:SMP0120840 Sucrase-Isomaltase Deficiency
PathBank:SMP0120835 Glycogen Synthetase Deficiency
PathBank:SMP0120839 Mucopolysaccharidosis VII. Sly Syndrome