EC: 3.1.4.3

phospholipase C (phosphatidylcholine cholinephosphohydrolase)

enzyme lambda metabolic reaction experiment
uniprot:P09598 [phospholipase C(Enzyme) wildtype]

H2O + Phosphatidylethanolamine -> Ethanolamine_phosphate + 1,2_Diacyl_sn_glycerol		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M 3,3-Dimethylglutaric acid, 0.167 mg/ml BSA
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) wildtype]

H2O + Phosphatidylcholine -> 1,2_Diacyl_sn_glycerol + Choline_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M 3,3-Dimethylglutaric acid, 0.167 mg/ml BSA
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) wildtype]

Phosphatidylserine + H2O -> 1,2_Diacyl_sn_glycerol + O_Phospho_L_serine		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M 3,3-Dimethylglutaric acid, 0.167 mg/ml BSA
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) wildtype]

Phosphatidylcholine + H2O -> 1,2_Diacyl_sn_glycerol + Choline_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant E4P/Y56S/F66N]

H2O + Phosphatidylcholine -> Choline_phosphate + 1,2_Diacyl_sn_glycerol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant Y56L/F66L]

Phosphatidylcholine + H2O -> 1,2_Diacyl_sn_glycerol + Choline_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant E4Q/Y56T/F66Y]

Phosphatidylcholine + H2O -> Choline_phosphate + 1,2_Diacyl_sn_glycerol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant E4R/Y56S/F66N]

H2O + Phosphatidylcholine -> Choline_phosphate + 1,2_Diacyl_sn_glycerol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant E4G]

H2O + Phosphatidylcholine -> Choline_phosphate + 1,2_Diacyl_sn_glycerol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant Y56T/F66P]

Phosphatidylcholine + H2O -> Choline_phosphate + 1,2_Diacyl_sn_glycerol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant E4K/Y56V]

Phosphatidylcholine + H2O -> 1,2_Diacyl_sn_glycerol + Choline_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant E4P/Y56S/F66N]

Phosphatidylethanolamine + H2O -> Ethanolamine_phosphate + 1,2_Diacyl_sn_glycerol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) wildtype]

Phosphatidylethanolamine + H2O -> 1,2_Diacyl_sn_glycerol + Ethanolamine_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant E4G]

H2O + Phosphatidylethanolamine -> 1,2_Diacyl_sn_glycerol + Ethanolamine_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant Y56T/F66P]

H2O + Phosphatidylethanolamine -> 1,2_Diacyl_sn_glycerol + Ethanolamine_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant E4Q/Y56T/F66Y]

Phosphatidylethanolamine + H2O -> Ethanolamine_phosphate + 1,2_Diacyl_sn_glycerol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant Y56L/F66L]

Phosphatidylethanolamine + H2O -> Ethanolamine_phosphate + 1,2_Diacyl_sn_glycerol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant E4K/Y56V]

Phosphatidylethanolamine + H2O -> 1,2_Diacyl_sn_glycerol + Ethanolamine_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant E4Q/Y56T]

Phosphatidylcholine + H2O -> 1,2_Diacyl_sn_glycerol + Choline_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant Y56T/F66Y]

H2O + Phosphatidylcholine -> Choline_phosphate + 1,2_Diacyl_sn_glycerol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant E4Q/F66Y]

H2O + Phosphatidylethanolamine -> 1,2_Diacyl_sn_glycerol + Ethanolamine_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant E4Q/Y56T]

Phosphatidylethanolamine + H2O -> 1,2_Diacyl_sn_glycerol + Ethanolamine_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant Y56T/F66Y]

Phosphatidylethanolamine + H2O -> 1,2_Diacyl_sn_glycerol + Ethanolamine_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) wildtype]

Phosphatidylserine + H2O -> O_Phospho_L_serine + 1,2_Diacyl_sn_glycerol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant E4G]

H2O + Phosphatidylserine -> 1,2_Diacyl_sn_glycerol + O_Phospho_L_serine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant Y56L/F66L]

H2O + Phosphatidylserine -> 1,2_Diacyl_sn_glycerol + O_Phospho_L_serine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant E4K/Y56V]

H2O + Phosphatidylserine -> 1,2_Diacyl_sn_glycerol + O_Phospho_L_serine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant Y56T/F66P]

Phosphatidylserine + H2O -> O_Phospho_L_serine + 1,2_Diacyl_sn_glycerol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant E4Q/Y56T/F66Y]

Phosphatidylserine + H2O -> O_Phospho_L_serine + 1,2_Diacyl_sn_glycerol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant E4Q]

H2O + Phosphatidylserine -> O_Phospho_L_serine + 1,2_Diacyl_sn_glycerol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant E4Q/Y56T]

Phosphatidylserine + H2O -> O_Phospho_L_serine + 1,2_Diacyl_sn_glycerol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant Y56T/F66Y]

Phosphatidylserine + H2O -> O_Phospho_L_serine + 1,2_Diacyl_sn_glycerol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant E4Q/F66Y]

Phosphatidylserine + H2O -> 1,2_Diacyl_sn_glycerol + O_Phospho_L_serine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant E4K]

Phosphatidylcholine + H2O -> 1,2_Diacyl_sn_glycerol + Choline_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant Y56V]

H2O + Phosphatidylcholine -> Choline_phosphate + 1,2_Diacyl_sn_glycerol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant Y56T]

H2O + Phosphatidylcholine -> Choline_phosphate + 1,2_Diacyl_sn_glycerol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant F66Y]

H2O + Phosphatidylcholine -> Choline_phosphate + 1,2_Diacyl_sn_glycerol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant E4Q/F66Y]

Phosphatidylcholine + H2O -> Choline_phosphate + 1,2_Diacyl_sn_glycerol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant E4G/Y56I/F66N]

Phosphatidylcholine + H2O -> Choline_phosphate + 1,2_Diacyl_sn_glycerol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant E4D/Y56D/F66N]

H2O + Phosphatidylcholine -> Choline_phosphate + 1,2_Diacyl_sn_glycerol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant E4G/Y56T/F66N]

H2O + Phosphatidylcholine -> 1,2_Diacyl_sn_glycerol + Choline_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant E4Q]

Phosphatidylcholine + H2O -> Choline_phosphate + 1,2_Diacyl_sn_glycerol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant Y56V]

H2O + Phosphatidylethanolamine -> Ethanolamine_phosphate + 1,2_Diacyl_sn_glycerol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant F66Y]

Phosphatidylethanolamine + H2O -> Ethanolamine_phosphate + 1,2_Diacyl_sn_glycerol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant E4Q]

Phosphatidylethanolamine + H2O -> 1,2_Diacyl_sn_glycerol + Ethanolamine_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant E4K]

H2O + Phosphatidylethanolamine -> Ethanolamine_phosphate + 1,2_Diacyl_sn_glycerol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant Y56T]

Phosphatidylethanolamine + H2O -> Ethanolamine_phosphate + 1,2_Diacyl_sn_glycerol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant E4G/Y56I/F66N]

Phosphatidylethanolamine + H2O -> 1,2_Diacyl_sn_glycerol + Ethanolamine_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant E4G/Y56T/F66N]

Phosphatidylethanolamine + H2O -> 1,2_Diacyl_sn_glycerol + Ethanolamine_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant E4R/Y56S/F66N]

Phosphatidylethanolamine + H2O -> Ethanolamine_phosphate + 1,2_Diacyl_sn_glycerol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant E4D/Y56D/F66N]

Phosphatidylethanolamine + H2O -> 1,2_Diacyl_sn_glycerol + Ethanolamine_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant E4P/Y56S/F66N]

H2O + Phosphatidylserine -> O_Phospho_L_serine + 1,2_Diacyl_sn_glycerol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant E4R/Y56S/F66N]

Phosphatidylserine + H2O -> 1,2_Diacyl_sn_glycerol + O_Phospho_L_serine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant E4G/Y56I/F66N]

Phosphatidylserine + H2O -> O_Phospho_L_serine + 1,2_Diacyl_sn_glycerol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant F66Y]

Phosphatidylserine + H2O -> O_Phospho_L_serine + 1,2_Diacyl_sn_glycerol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant E4D/Y56D/F66N]

Phosphatidylserine + H2O -> 1,2_Diacyl_sn_glycerol + O_Phospho_L_serine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant E4K]

H2O + Phosphatidylserine -> O_Phospho_L_serine + 1,2_Diacyl_sn_glycerol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant Y56V]

Phosphatidylserine + H2O -> 1,2_Diacyl_sn_glycerol + O_Phospho_L_serine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant Y56T]

H2O + Phosphatidylserine -> 1,2_Diacyl_sn_glycerol + O_Phospho_L_serine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37
uniprot:P09598 [phospholipase C(Enzyme) mutant E4G/Y56T/F66N]

Phosphatidylserine + H2O -> 1,2_Diacyl_sn_glycerol + O_Phospho_L_serine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM 3,3-Dimethylglutaric acid, 0.03 mM ZnSO4, 0.056 mg/ml bovine serum albumin
PH: 7.3
Temperature: 37

Pathways

pathway id name
BioCyc:ARA_LIPASYN-PWY phospholipases
BioCyc:MTBH37RV_LIPASYN-PWY phospholipases
BioCyc:META_LIPASYN-PWY phospholipases
BioCyc:MOUSE_PWY3DJ-11281 sphingomyelin metabolism
PlantCyc:ARA_LIPASYN-PWY phospholipases
PlantCyc:PLANT_LIPASYN-PWY phospholipases
Reactome:R-BTA-9840310 Glycosphingolipid catabolism
Reactome:R-HSA-9840310 Glycosphingolipid catabolism
Reactome:R-MMU-9840310 Glycosphingolipid catabolism
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-556833 Metabolism of lipids
Reactome:R-BTA-428157 Sphingolipid metabolism
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-556833 Metabolism of lipids
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-HSA-428157 Sphingolipid metabolism
Reactome:R-MMU-1660662 Glycosphingolipid metabolism
Reactome:R-HSA-1660662 Glycosphingolipid metabolism
Reactome:R-MMU-428157 Sphingolipid metabolism
Reactome:R-BTA-1660662 Glycosphingolipid metabolism
Reactome:R-HSA-1430728 Metabolism
WikiPathways:WP4725 Sphingolipid metabolism overview
PathBank:SMP0071721 Phosphatidylethanolamine Biosynthesis PE(16:0/18:0)
PathBank:SMP0071725 Phosphatidylethanolamine Biosynthesis PE(16:0/18:3(6Z,9Z,12Z))
PathBank:SMP0071729 Phosphatidylethanolamine Biosynthesis PE(16:0/20:1(13Z))
PathBank:SMP0071733 Phosphatidylethanolamine Biosynthesis PE(18:0/18:1(9Z))
PathBank:SMP0071737 Phosphatidylethanolamine Biosynthesis PE(18:0/18:3(9Z,12Z,15Z))
PathBank:SMP0071741 Phosphatidylethanolamine Biosynthesis PE(18:0/22:0)
PathBank:SMP0071745 Phosphatidylethanolamine Biosynthesis PE(18:1(9Z)/18:2(9Z,12Z))
PathBank:SMP0071749 Phosphatidylethanolamine Biosynthesis PE(18:1(9Z)/20:1(11Z))
PathBank:SMP0071753 Phosphatidylethanolamine Biosynthesis PE(18:1(11Z)/18:1(11Z))
PathBank:SMP0071757 Phosphatidylethanolamine Biosynthesis PE(18:1(11Z)/20:0)
PathBank:SMP0071761 Phosphatidylethanolamine Biosynthesis PE(18:1(11Z)/22:1(13Z))
PathBank:SMP0071765 Phosphatidylethanolamine Biosynthesis PE(18:2(9Z,12Z)/20:0)
PathBank:SMP0071769 Phosphatidylethanolamine Biosynthesis PE(18:2(9Z,12Z)/22:1(13Z))
PathBank:SMP0071773 Phosphatidylethanolamine Biosynthesis PE(18:3(6Z,9Z,12Z)/20:1(11Z))
PathBank:SMP0071777 Phosphatidylethanolamine Biosynthesis PE(18:3(9Z,12Z,15Z)/18:3(9Z,12Z,15Z))
PathBank:SMP0071781 Phosphatidylethanolamine Biosynthesis PE(18:3(9Z,12Z,15Z)/22:0)
PathBank:SMP0071785 Phosphatidylethanolamine Biosynthesis PE(20:0/20:1(13Z))
PathBank:SMP0071789 Phosphatidylethanolamine Biosynthesis PE(20:1(11Z)/20:1(13Z))
PathBank:SMP0071793 Phosphatidylethanolamine Biosynthesis PE(20:1(13Z)/22:0)
PathBank:SMP0071797 Phosphatidylethanolamine Biosynthesis PE(22:1(13Z)/22:1(13Z))
PathBank:SMP0063821 Phosphatidylcholine Biosynthesis PC(16:0/18:1(11Z))
PathBank:SMP0063825 Phosphatidylcholine Biosynthesis PC(16:0/20:0)
PathBank:SMP0063829 Phosphatidylcholine Biosynthesis PC(16:0/22:1(13Z))
PathBank:SMP0063833 Phosphatidylcholine Biosynthesis PC(18:0/18:2(9Z,12Z))
PathBank:SMP0063837 Phosphatidylcholine Biosynthesis PC(18:0/20:1(11Z))
PathBank:SMP0063841 Phosphatidylcholine Biosynthesis PC(18:1(9Z)/18:1(9Z))
PathBank:SMP0063845 Phosphatidylcholine Biosynthesis PC(18:1(9Z)/18:3(9Z,12Z,15Z))
PathBank:SMP0063849 Phosphatidylcholine Biosynthesis PC(18:1(9Z)/22:0)
PathBank:SMP0063853 Phosphatidylcholine Biosynthesis PC(18:1(11Z)/18:3(6Z,9Z,12Z))
PathBank:SMP0063857 Phosphatidylcholine Biosynthesis PC(18:1(11Z)/20:1(13Z))
PathBank:SMP0063861 Phosphatidylcholine Biosynthesis PC(18:2(9Z,12Z)/18:3(6Z,9Z,12Z))
PathBank:SMP0063865 Phosphatidylcholine Biosynthesis PC(18:2(9Z,12Z)/20:1(13Z))
PathBank:SMP0063869 Phosphatidylcholine Biosynthesis PC(18:3(6Z,9Z,12Z)/18:3(9Z,12Z,15Z))
PathBank:SMP0063873 Phosphatidylcholine Biosynthesis PC(18:3(6Z,9Z,12Z)/22:0)
PathBank:SMP0063877 Phosphatidylcholine Biosynthesis PC(18:3(9Z,12Z,15Z)/20:1(11Z))
PathBank:SMP0063881 Phosphatidylcholine Biosynthesis PC(20:0/20:0)
PathBank:SMP0063885 Phosphatidylcholine Biosynthesis PC(20:0/22:1(13Z))
PathBank:SMP0063889 Phosphatidylcholine Biosynthesis PC(20:1(11Z)/22:1(13Z))
PathBank:SMP0063893 Phosphatidylcholine Biosynthesis PC(22:0/22:0)
PathBank:SMP0071720 Phosphatidylethanolamine Biosynthesis PE(16:0/16:0)
PathBank:SMP0071724 Phosphatidylethanolamine Biosynthesis PE(16:0/18:2(9Z,12Z))
PathBank:SMP0071728 Phosphatidylethanolamine Biosynthesis PE(16:0/20:1(11Z))
PathBank:SMP0071732 Phosphatidylethanolamine Biosynthesis PE(18:0/18:0)
PathBank:SMP0071736 Phosphatidylethanolamine Biosynthesis PE(18:0/18:3(6Z,9Z,12Z))
PathBank:SMP0071740 Phosphatidylethanolamine Biosynthesis PE(18:0/20:1(13Z))
PathBank:SMP0071744 Phosphatidylethanolamine Biosynthesis PE(18:1(9Z)/18:1(11Z))
PathBank:SMP0071748 Phosphatidylethanolamine Biosynthesis PE(18:1(9Z)/20:0)
PathBank:SMP0071752 Phosphatidylethanolamine Biosynthesis PE(18:1(9Z)/22:1(13Z))
PathBank:SMP0071756 Phosphatidylethanolamine Biosynthesis PE(18:1(11Z)/18:3(9Z,12Z,15Z))
PathBank:SMP0071760 Phosphatidylethanolamine Biosynthesis PE(18:1(11Z)/22:0)
PathBank:SMP0071764 Phosphatidylethanolamine Biosynthesis PE(18:2(9Z,12Z)/18:3(9Z,12Z,15Z))
PathBank:SMP0071768 Phosphatidylethanolamine Biosynthesis PE(18:2(9Z,12Z)/22:0)
PathBank:SMP0071772 Phosphatidylethanolamine Biosynthesis PE(18:3(6Z,9Z,12Z)/20:0)
PathBank:SMP0071776 Phosphatidylethanolamine Biosynthesis PE(18:3(6Z,9Z,12Z)/22:1(13Z))
PathBank:SMP0071780 Phosphatidylethanolamine Biosynthesis PE(18:3(9Z,12Z,15Z)/20:1(13Z))
PathBank:SMP0071784 Phosphatidylethanolamine Biosynthesis PE(20:0/20:1(11Z))
PathBank:SMP0071788 Phosphatidylethanolamine Biosynthesis PE(20:1(11Z)/20:1(11Z))
PathBank:SMP0071792 Phosphatidylethanolamine Biosynthesis PE(20:1(13Z)/20:1(13Z))
PathBank:SMP0071796 Phosphatidylethanolamine Biosynthesis PE(22:0/22:1(13Z))
PathBank:SMP0063809 Phosphatidylcholine Biosynthesis PC(16:0/18:0)
PathBank:SMP0063820 Phosphatidylcholine Biosynthesis PC(16:0/18:1(9Z))
PathBank:SMP0063824 Phosphatidylcholine Biosynthesis PC(16:0/18:3(9Z,12Z,15Z))
PathBank:SMP0063828 Phosphatidylcholine Biosynthesis PC(16:0/22:0)
PathBank:SMP0063832 Phosphatidylcholine Biosynthesis PC(18:0/18:1(11Z))
PathBank:SMP0063836 Phosphatidylcholine Biosynthesis PC(18:0/20:0)
PathBank:SMP0063840 Phosphatidylcholine Biosynthesis PC(18:0/22:1(13Z))
PathBank:SMP0063844 Phosphatidylcholine Biosynthesis PC(18:1(9Z)/18:3(6Z,9Z,12Z))
PathBank:SMP0063848 Phosphatidylcholine Biosynthesis PC(18:1(9Z)/20:1(13Z))
PathBank:SMP0063852 Phosphatidylcholine Biosynthesis PC(18:1(11Z)/18:2(9Z,12Z))
PathBank:SMP0063856 Phosphatidylcholine Biosynthesis PC(18:1(11Z)/20:1(11Z))
PathBank:SMP0063860 Phosphatidylcholine Biosynthesis PC(18:2(9Z,12Z)/18:2(9Z,12Z))
PathBank:SMP0063864 Phosphatidylcholine Biosynthesis PC(18:2(9Z,12Z)/20:1(11Z))
PathBank:SMP0063868 Phosphatidylcholine Biosynthesis PC(18:3(6Z,9Z,12Z)/18:3(6Z,9Z,12Z))
PathBank:SMP0063872 Phosphatidylcholine Biosynthesis PC(18:3(6Z,9Z,12Z)/20:1(13Z))
PathBank:SMP0063876 Phosphatidylcholine Biosynthesis PC(18:3(9Z,12Z,15Z)/20:0)
PathBank:SMP0063880 Phosphatidylcholine Biosynthesis PC(18:3(9Z,12Z,15Z)/22:1(13Z))
PathBank:SMP0063884 Phosphatidylcholine Biosynthesis PC(20:0/22:0)
PathBank:SMP0063888 Phosphatidylcholine Biosynthesis PC(20:1(11Z)/22:0)
PathBank:SMP0063892 Phosphatidylcholine Biosynthesis PC(20:1(13Z)/22:1(13Z))
PathBank:SMP0071723 Phosphatidylethanolamine Biosynthesis PE(16:0/18:1(11Z))
PathBank:SMP0071727 Phosphatidylethanolamine Biosynthesis PE(16:0/20:0)
PathBank:SMP0071731 Phosphatidylethanolamine Biosynthesis PE(16:0/22:1(13Z))
PathBank:SMP0071735 Phosphatidylethanolamine Biosynthesis PE(18:0/18:2(9Z,12Z))
PathBank:SMP0071739 Phosphatidylethanolamine Biosynthesis PE(18:0/20:1(11Z))
PathBank:SMP0071743 Phosphatidylethanolamine Biosynthesis PE(18:1(9Z)/18:1(9Z))
PathBank:SMP0071747 Phosphatidylethanolamine Biosynthesis PE(18:1(9Z)/18:3(9Z,12Z,15Z))
PathBank:SMP0071751 Phosphatidylethanolamine Biosynthesis PE(18:1(9Z)/22:0)
PathBank:SMP0071755 Phosphatidylethanolamine Biosynthesis PE(18:1(11Z)/18:3(6Z,9Z,12Z))
PathBank:SMP0071759 Phosphatidylethanolamine Biosynthesis PE(18:1(11Z)/20:1(13Z))
PathBank:SMP0071763 Phosphatidylethanolamine Biosynthesis PE(18:2(9Z,12Z)/18:3(6Z,9Z,12Z))
PathBank:SMP0071767 Phosphatidylethanolamine Biosynthesis PE(18:2(9Z,12Z)/20:1(13Z))
PathBank:SMP0071771 Phosphatidylethanolamine Biosynthesis PE(18:3(6Z,9Z,12Z)/18:3(9Z,12Z,15Z))
PathBank:SMP0071775 Phosphatidylethanolamine Biosynthesis PE(18:3(6Z,9Z,12Z)/22:0)
PathBank:SMP0071779 Phosphatidylethanolamine Biosynthesis PE(18:3(9Z,12Z,15Z)/20:1(11Z))
PathBank:SMP0071783 Phosphatidylethanolamine Biosynthesis PE(20:0/20:0)
PathBank:SMP0071787 Phosphatidylethanolamine Biosynthesis PE(20:0/22:1(13Z))
PathBank:SMP0071791 Phosphatidylethanolamine Biosynthesis PE(20:1(11Z)/22:1(13Z))
PathBank:SMP0071795 Phosphatidylethanolamine Biosynthesis PE(22:0/22:0)
PathBank:SMP0014205 Phosphatidylcholine Biosynthesis
PathBank:SMP0063808 Phosphatidylcholine Biosynthesis PC(16:0/16:0)
PathBank:SMP0063823 Phosphatidylcholine Biosynthesis PC(16:0/18:3(6Z,9Z,12Z))
PathBank:SMP0063827 Phosphatidylcholine Biosynthesis PC(16:0/20:1(13Z))
PathBank:SMP0063831 Phosphatidylcholine Biosynthesis PC(18:0/18:1(9Z))
PathBank:SMP0063835 Phosphatidylcholine Biosynthesis PC(18:0/18:3(9Z,12Z,15Z))
PathBank:SMP0063839 Phosphatidylcholine Biosynthesis PC(18:0/22:0)
PathBank:SMP0063843 Phosphatidylcholine Biosynthesis PC(18:1(9Z)/18:2(9Z,12Z))
PathBank:SMP0063847 Phosphatidylcholine Biosynthesis PC(18:1(9Z)/20:1(11Z))
PathBank:SMP0063851 Phosphatidylcholine Biosynthesis PC(18:1(11Z)/18:1(11Z))
PathBank:SMP0063855 Phosphatidylcholine Biosynthesis PC(18:1(11Z)/20:0)
PathBank:SMP0063859 Phosphatidylcholine Biosynthesis PC(18:1(11Z)/22:1(13Z))
PathBank:SMP0063863 Phosphatidylcholine Biosynthesis PC(18:2(9Z,12Z)/20:0)
PathBank:SMP0063867 Phosphatidylcholine Biosynthesis PC(18:2(9Z,12Z)/22:1(13Z))
PathBank:SMP0063871 Phosphatidylcholine Biosynthesis PC(18:3(6Z,9Z,12Z)/20:1(11Z))
PathBank:SMP0063875 Phosphatidylcholine Biosynthesis PC(18:3(9Z,12Z,15Z)/18:3(9Z,12Z,15Z))
PathBank:SMP0063879 Phosphatidylcholine Biosynthesis PC(18:3(9Z,12Z,15Z)/22:0)
PathBank:SMP0063883 Phosphatidylcholine Biosynthesis PC(20:0/20:1(13Z))
PathBank:SMP0063887 Phosphatidylcholine Biosynthesis PC(20:1(11Z)/20:1(13Z))
PathBank:SMP0063891 Phosphatidylcholine Biosynthesis PC(20:1(13Z)/22:0)
PathBank:SMP0063895 Phosphatidylcholine Biosynthesis PC(22:1(13Z)/22:1(13Z))
PathBank:SMP0071722 Phosphatidylethanolamine Biosynthesis PE(16:0/18:1(9Z))
PathBank:SMP0071726 Phosphatidylethanolamine Biosynthesis PE(16:0/18:3(9Z,12Z,15Z))
PathBank:SMP0071730 Phosphatidylethanolamine Biosynthesis PE(16:0/22:0)
PathBank:SMP0071734 Phosphatidylethanolamine Biosynthesis PE(18:0/18:1(11Z))
PathBank:SMP0071738 Phosphatidylethanolamine Biosynthesis PE(18:0/20:0)
PathBank:SMP0071742 Phosphatidylethanolamine Biosynthesis PE(18:0/22:1(13Z))
PathBank:SMP0071746 Phosphatidylethanolamine Biosynthesis PE(18:1(9Z)/18:3(6Z,9Z,12Z))
PathBank:SMP0071750 Phosphatidylethanolamine Biosynthesis PE(18:1(9Z)/20:1(13Z))
PathBank:SMP0071754 Phosphatidylethanolamine Biosynthesis PE(18:1(11Z)/18:2(9Z,12Z))
PathBank:SMP0071758 Phosphatidylethanolamine Biosynthesis PE(18:1(11Z)/20:1(11Z))
PathBank:SMP0071762 Phosphatidylethanolamine Biosynthesis PE(18:2(9Z,12Z)/18:2(9Z,12Z))
PathBank:SMP0071766 Phosphatidylethanolamine Biosynthesis PE(18:2(9Z,12Z)/20:1(11Z))
PathBank:SMP0071770 Phosphatidylethanolamine Biosynthesis PE(18:3(6Z,9Z,12Z)/18:3(6Z,9Z,12Z))
PathBank:SMP0071774 Phosphatidylethanolamine Biosynthesis PE(18:3(6Z,9Z,12Z)/20:1(13Z))
PathBank:SMP0071778 Phosphatidylethanolamine Biosynthesis PE(18:3(9Z,12Z,15Z)/20:0)
PathBank:SMP0071782 Phosphatidylethanolamine Biosynthesis PE(18:3(9Z,12Z,15Z)/22:1(13Z))
PathBank:SMP0071786 Phosphatidylethanolamine Biosynthesis PE(20:0/22:0)
PathBank:SMP0071790 Phosphatidylethanolamine Biosynthesis PE(20:1(11Z)/22:0)
PathBank:SMP0071794 Phosphatidylethanolamine Biosynthesis PE(20:1(13Z)/22:1(13Z))
PathBank:SMP0014208 Phosphatidylethanolamine Biosynthesis
PathBank:SMP0063822 Phosphatidylcholine Biosynthesis PC(16:0/18:2(9Z,12Z))
PathBank:SMP0063826 Phosphatidylcholine Biosynthesis PC(16:0/20:1(11Z))
PathBank:SMP0063830 Phosphatidylcholine Biosynthesis PC(18:0/18:0)
PathBank:SMP0063834 Phosphatidylcholine Biosynthesis PC(18:0/18:3(6Z,9Z,12Z))
PathBank:SMP0063838 Phosphatidylcholine Biosynthesis PC(18:0/20:1(13Z))
PathBank:SMP0063842 Phosphatidylcholine Biosynthesis PC(18:1(9Z)/18:1(11Z))
PathBank:SMP0063846 Phosphatidylcholine Biosynthesis PC(18:1(9Z)/20:0)
PathBank:SMP0063850 Phosphatidylcholine Biosynthesis PC(18:1(9Z)/22:1(13Z))
PathBank:SMP0063854 Phosphatidylcholine Biosynthesis PC(18:1(11Z)/18:3(9Z,12Z,15Z))
PathBank:SMP0063858 Phosphatidylcholine Biosynthesis PC(18:1(11Z)/22:0)
PathBank:SMP0063862 Phosphatidylcholine Biosynthesis PC(18:2(9Z,12Z)/18:3(9Z,12Z,15Z))
PathBank:SMP0063866 Phosphatidylcholine Biosynthesis PC(18:2(9Z,12Z)/22:0)
PathBank:SMP0063870 Phosphatidylcholine Biosynthesis PC(18:3(6Z,9Z,12Z)/20:0)
PathBank:SMP0063874 Phosphatidylcholine Biosynthesis PC(18:3(6Z,9Z,12Z)/22:1(13Z))
PathBank:SMP0063878 Phosphatidylcholine Biosynthesis PC(18:3(9Z,12Z,15Z)/20:1(13Z))
PathBank:SMP0063882 Phosphatidylcholine Biosynthesis PC(20:0/20:1(11Z))
PathBank:SMP0063886 Phosphatidylcholine Biosynthesis PC(20:1(11Z)/20:1(11Z))
PathBank:SMP0063890 Phosphatidylcholine Biosynthesis PC(20:1(13Z)/20:1(13Z))
PathBank:SMP0063894 Phosphatidylcholine Biosynthesis PC(22:0/22:1(13Z))