EC: 3.1.3.25

inositol-phosphate phosphatase (myo-inositol-phosphate phosphohydrolase)

enzyme lambda metabolic reaction experiment
uniprot:P20456 [Mg2+]

1D_myo_Inositol_3_phosphate + H2O -> Phosphate + myo_Inositol		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris/HCl, 250 mM KCl, 1 mM inosine, 1 mM 2-p-iodophenyl-3-p-nitrophenyl-5-phenyltetrazolium, 0.5 U/ml of nucleoside phosphorylase, 0.05 U/ml of xanthine oxidase
PH: 8
Temperature: 37
uniprot:P20456 [Mg2+]

H2O + 1D_myo_Inositol_1_phosphate -> myo_Inositol + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris/HCl, 250 mM KCl, 1 mM inosine, 1 mM 2-p-iodophenyl-3-p-nitrophenyl-5-phenyltetrazolium, 0.5 U/ml of nucleoside phosphorylase, 0.05 U/ml of xanthine oxidase
PH: 8
Temperature: 37
uniprot:P20456 [Mg2+]

1D_myo_Inositol_1_phosphate + H2O -> myo_Inositol + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris/HCl, 250 mM KCl, 1 mM inosine, 1 mM 2-p-iodophenyl-3-p-nitrophenyl-5-phenyltetrazolium, 0.5 U/ml of nucleoside phosphorylase, 0.05 U/ml of xanthine oxidase
PH: 8
Temperature: 37
uniprot:P20456 [Mg2+]

H2O + myo_Inositol_4_phosphate -> myo_Inositol + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris/HCl, 250 mM KCl, 1 mM inosine, 1 mM 2-p-iodophenyl-3-p-nitrophenyl-5-phenyltetrazolium, 0.5 U/ml of nucleoside phosphorylase, 0.05 U/ml of xanthine oxidase
PH: 8
Temperature: 37
uniprot:P20456 [Mg2+]

H2O + myo_Inositol_4_phosphate -> myo_Inositol + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris/HCl, 250 mM KCl, 1 mM inosine, 1 mM 2-p-iodophenyl-3-p-nitrophenyl-5-phenyltetrazolium, 0.5 U/ml of nucleoside phosphorylase, 0.05 U/ml of xanthine oxidase
PH: 8
Temperature: 37
uniprot:P20456 [Mg2+]

myo_Inositol_4_phosphate + H2O -> Phosphate + myo_Inositol		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris/HCl, 250 mM KCl, 1 mM inosine, 1 mM 2-p-iodophenyl-3-p-nitrophenyl-5-phenyltetrazolium, 0.5 U/ml of nucleoside phosphorylase, 0.05 U/ml of xanthine oxidase
PH: 8
Temperature: 37
- [Mg2+]

1D_myo_Inositol_3_phosphate + H2O -> myo_Inositol + Phosphate		 (( (Vmax * S) ) / (S + (Km * (1 + (I / Ki))))) buffer: 120 mM KCl, 10 mM NaCl, 1 mM EGTA, 0.33 mM CaCl2 (0.1 µM free Ca2+), 20 mM Hepes, 0.2 mg of saponin/ml.
PH: 7.2
Temperature: 37
- [Mg2+]

1D_myo_Inositol_3_phosphate + H2O -> myo_Inositol + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 120 mM KCl, 10 mM NaCl, 1 mM EGTA, 0.33 mM CaCl2 (0.1 µM free Ca2+), 20 mM Hepes, 0.2 mg of saponin/ml.
PH: 7.2
Temperature: 37
- [Mg2+]

myo_Inositol_4_phosphate + H2O -> Phosphate + myo_Inositol		 (( (Vmax * S) ) / (Km + S)) buffer: 120 mM KCl, 10 mM NaCl, 1 mM EGTA, 0.33 mM CaCl2 (0.1 µM free Ca2+), 20 mM Hepes, 0.2 mg of saponin/ml.
PH: 7.2
Temperature: 37
- [inositol-phosphate phosphatase(Enzyme) wildtype]

myo_Inositol_phosphate + H2O -> Phosphate + myo_Inositol		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Hepes, 2 mM MgCl2, 5 mM mercaptoethanol
PH: 7.4
Temperature: 37
uniprot:P65163 [Mg2+]

myo_Inositol_phosphate + H2O -> myo_Inositol + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl
PH: 9.3
Temperature: 37
uniprot:P29218 [Galactose 1-phosphate]

myo_Inositol_phosphate + H2O -> Phosphate + myo_Inositol		 (( (Vmax * A) ) / (Km + A)) buffer: 0.4 M K+ phosphate, 10 mM NAD+, 8 mM bathophenanthroline disulphonic acid, 1.6 mM FeCl3, 25.2 µM phenazine methosulphate
PH: 9
Temperature: 25
uniprot:P29218 [inositol-phosphate phosphatase(Enzyme) wildtype]

H2O + myo_Inositol_phosphate -> Phosphate + myo_Inositol		 (( (Vmax * A) ) / (Km + A)) buffer: 0.4 M K+ phosphate, 10 mM NAD+, 8 mM bathophenanthroline disulphonic acid, 1.6 mM FeCl3, 25.2 µM phenazine methosulphate
PH: 9
Temperature: 25
uniprot:Q5U789 [Mg2+]

1D_myo_Inositol_1_phosphate + H2O -> Phosphate + myo_Inositol		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Bis-Tris propane
PH: 7
Temperature: 30
uniprot:Q05533 [Mg2+]

H2O + 1D_myo_Inositol_1_phosphate -> Phosphate + myo_Inositol		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl
PH: 7.6
Temperature: 30
uniprot:P38710 [Mg2+]

H2O + 1D_myo_Inositol_1_phosphate -> myo_Inositol + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl
PH: 7.6
Temperature: 30
uniprot:Q9Y2H2 [inositol-phosphate phosphatase(Enzyme) wildtype GST-tagged]

H2O + 1_Phosphatidyl_D_myo_inositol_4,5_bisphosphate -> 1_Phosphatidyl_1D_myo_inositol_4_phosphate + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 10 mM dithiothreitol
PH: 8
Temperature: 37
uniprot:P29218 [Mg2+]

H2O + 1D_myo_Inositol_1_phosphate -> myo_Inositol + Phosphate		 (( (( (kcat * E) ) * (M ^ n)) ) / (( (M_half ^ n) ) + (( (M ^ n) ) * (1 + (M / Ki))))) buffer: 50 mM Tris-HCl, 0.1 mM EGTA
PH: 7.5
Temperature: 37
uniprot:P29218 [Mg2+]

H2O + 1D_myo_Inositol_1_phosphate -> myo_Inositol + Phosphate		 (( (( (kcat * E) ) * (M ^ n)) ) / (( (M_half ^ n) ) + (( (M ^ n) ) * (1 + (M / Ki))))) buffer: 50 mM Tris-HCl, 0.1 mM EGTA
PH: 7.5
Temperature: 37
uniprot:P29218 [Mg2+]

1D_myo_Inositol_1_phosphate + H2O -> myo_Inositol + Phosphate		 (Vmax / (( (( (Kia * Kb) ) * Ka) ) / (( (( (( (( (A ^ 3) ) * B) ) + (( (( (Kb * Ka) ) / A) ) * B)) ) + (Ka / A)) ) + 1))) buffer: 50 mM Tris-HCl, 0.1 mM EGTA
PH: 7.5
Temperature: 37
uniprot:P29218 [Mg2+]

H2O + 1D_myo_Inositol_1_phosphate -> Phosphate + myo_Inositol		 (Vmax / (( (( (Kia * Kb) ) * Ka) ) / (( (( (( (( (A ^ 2) ) * B) ) + (( (( (Kb * Ka) ) / A) ) * B)) ) + (Ka / A)) ) + 1))) buffer: 50 mM Tris-HCl, 0.1 mM EGTA
PH: 7.5
Temperature: 37
uniprot:P29218 [Mg2+]

H2O + 1D_myo_Inositol_1_phosphate -> myo_Inositol + Phosphate		 (Vmax / (( (( (Kia * Kb) ) * Ka) ) / (( (( (( (( (A ^ 3) ) * B) ) + (( (( (Kb * Ka) ) / A) ) * B)) ) + (Ka / A)) ) + 1))) buffer: 50 mM Tris-HCl, 0.1 mM EGTA
PH: 7.5
Temperature: 37
uniprot:P29218 [Mg2+]

H2O + 1D_myo_Inositol_1_phosphate -> Phosphate + myo_Inositol		 (Vmax / (( (( (Kia * Kb) ) * Ka) ) / (( (( (( (( (A ^ 2) ) * B) ) + (( (( (Kb * Ka) ) / A) ) * B)) ) + (Ka / A)) ) + 1))) buffer: 50 mM Tris-HCl, 0.1 mM EGTA
PH: 7.5
Temperature: 37
- [inositol-phosphate phosphatase(Enzyme) wildtype]

H2O + 1D_myo_Inositol_3_phosphate -> myo_Inositol + Phosphate		 (k * S) buffer: 100 mM KCl, 10 mM NaCl, 10 mM HEPES, 1 mM EGTA, 3 mM MgSO4
PH: 7.2
Temperature: 37
- [inositol-phosphate phosphatase(Enzyme) wildtype]

H2O + 1D_myo_Inositol_1_phosphate -> myo_Inositol + Phosphate		 (k * S) buffer: 100 mM KCl, 10 mM NaCl, 10 mM HEPES, 1 mM EGTA, 3 mM MgSO4
PH: 7.2
Temperature: 37
- [inositol-phosphate phosphatase(Enzyme) wildtype]

H2O + myo_Inositol_4_phosphate -> myo_Inositol + Phosphate		 (k * S) buffer: 100 mM KCl, 10 mM NaCl, 10 mM HEPES, 1 mM EGTA, 3 mM MgSO4
PH: 7.2
Temperature: 37
- [inositol-phosphate phosphatase(Enzyme) wildtype]

H2O + 1D_myo_Inositol_1_phosphate -> myo_Inositol + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris/HCl, 250 mM KCl, 3 mM MgCl2, 1 mM inosine, 1 mM 2-p-iodophenyl-3-p-nitrophenyl-5-phenyltetrazolium, 0.5 U/ml of nucleoside phosphorylase, 0.05 U/ml of xanthine oxidase
PH: 8
Temperature: 37
- [inositol-phosphate phosphatase(Enzyme) wildtype]

1D_myo_Inositol_1_phosphate + H2O -> myo_Inositol + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris/HCl, 250 mM KCl, 3 mM MgCl2, 1 mM inosine, 1 mM 2-p-iodophenyl-3-p-nitrophenyl-5-phenyltetrazolium, 0.5 U/ml of nucleoside phosphorylase, 0.05 U/ml of xanthine oxidase
PH: 8
Temperature: 37
uniprot:P20456 [Mg2+]

H2O + 1D_myo_Inositol_1_phosphate -> myo_Inositol + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.05 M Tris/HCl, 0.1 mM EGTA
PH: 8
Temperature: 37
uniprot:P20456 [Li+]

1D_myo_Inositol_1_phosphate + H2O -> myo_Inositol + Phosphate		 (Vmax / (( (1 + (Km / A)) ) + (I / Ki))) buffer: 0.05 M Tris/HCl, 0.1 mM EGTA
PH: 8
Temperature: 37
uniprot:P20456 [Li+]

H2O + 4_Nitrophenyl_phosphate -> Phosphate + p_Nitrophenol		 (Vmax / (( (1 + (Km / A)) ) + (I / Ki))) buffer: 0.05 M Tris/HCl, 0.1 mM EGTA
PH: 8
Temperature: 37
uniprot:P20456 [Mg2+]

4_Nitrophenyl_phosphate + H2O -> p_Nitrophenol + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.05 M Tris/HCl, 0.1 mM EGTA
PH: 8
Temperature: 37
uniprot:P20456 [Li+]

Adenosine_2'_phosphate + H2O -> Adenosine + Phosphate		 (Vmax / (( (1 + (Km / A)) ) + (I / Ki))) buffer: 0.05 M Tris/HCl, 0.1 mM EGTA
PH: 8
Temperature: 37
uniprot:P20456 [Li+]

H2O + Glycerol_2_phosphate -> Phosphate + Glycerol		 (Vmax / (( (1 + (Km / A)) ) + (I / Ki))) buffer: 0.05 M Tris/HCl, 0.1 mM EGTA
PH: 8
Temperature: 37
uniprot:P20456 [Mg2+]

H2O + sn_Glycerol_1_phosphate -> Phosphate + Glycerol		 (( (Vmax * A) ) / (Km + A)) buffer: 0.05 M Tris/HCl, 0.1 mM EGTA
PH: 8
Temperature: 37
uniprot:P20456 [Mg2+]

Glycerol_2_phosphate + H2O -> Glycerol + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.05 M Tris/HCl, 0.1 mM EGTA
PH: 8
Temperature: 37
uniprot:P20456 [Mg2+]

H2O + Adenosine_2'_phosphate -> Phosphate + Adenosine		 (( (Vmax * A) ) / (Km + A)) buffer: 0.05 M Tris/HCl, 0.1 mM EGTA
PH: 8
Temperature: 37
uniprot:P20456 [Mg2+]

H2O + Propan_1_ol_2_phosphate -> Phosphate + 1_Propanol		 (( (Vmax * A) ) / (Km + A)) buffer: 0.05 M Tris/HCl, 0.1 mM EGTA
PH: 8
Temperature: 37
uniprot:P97697 [inositol-phosphate phosphatase(Enzyme) wildtype]

1D_myo_Inositol_3_phosphate + H2O -> Phosphate + myo_Inositol		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 250 mM KCl, 2 mM MgCl2
PH: 7.4
Temperature: 37
uniprot:P97697 [inositol-phosphate phosphatase(Enzyme) wildtype]

Glycerol_2_phosphate + H2O -> Glycerol + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 250 mM KCl, 2 mM MgCl2
PH: 7.4
Temperature: 37
uniprot:P97697 [inositol-phosphate phosphatase(Enzyme) wildtype]

H2O + Adenosine_2'_phosphate -> Phosphate + Adenosine		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 250 mM KCl, 2 mM MgCl2
PH: 7.4
Temperature: 37
uniprot:Q57573 [inositol-phosphate phosphatase(Enzyme) wildtype]

myo_Inositol_phosphate + H2O -> Phosphate + myo_Inositol		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 20 mM MgCl2
PH: 8.5
Temperature: 85
uniprot:O30298 [inositol-phosphate phosphatase(Enzyme) wildtype]

H2O + myo_Inositol_phosphate -> Phosphate + myo_Inositol		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 20 mM MgCl2
PH: 8.5
Temperature: 85

Pathways

pathway id name
BioCyc:META_PWY-6358 superpathway of D-myo-inositol (1,4,5)-trisphosphate metabolism
BioCyc:META_PWY-2301 myo-inositol biosynthesis
BioCyc:YEAST_PWY-2301 myo-inositol biosynthesis
BioCyc:ARA_HISTSYN-PWY L-histidine biosynthesis
BioCyc:MTBH37RV_PWY-2301 myo-inositol biosynthesis
BioCyc:MOUSE_PWY-2301 myo-inositol biosynthesis
BioCyc:META_PWY-6368 3-phosphoinositide degradation
BioCyc:META_HISTSYN-PWY L-histidine biosynthesis
BioCyc:META_PWY-882 L-ascorbate biosynthesis I (L-galactose pathway)
BioCyc:META_PWY-6363 D-myo-inositol (1,4,5)-trisphosphate degradation
BioCyc:MTBH37RV_GLUCONEO-PWY gluconeogenesis I
BioCyc:MOUSE_PWY-6358 superpathway of D-myo-inositol (1,4,5)-trisphosphate metabolism
BioCyc:HUMAN_PWY-6368 3-phosphoinositide degradation
BioCyc:HUMAN_PWY-6363 D-myo-inositol (1,4,5)-trisphosphate degradation
BioCyc:HUMAN_PWY-6358 superpathway of D-myo-inositol (1,4,5)-trisphosphate metabolism
BioCyc:HUMAN_PWY-2301 myo-inositol de novo biosynthesis
BioCyc:ARA_PWY-2301 myo-inositol biosynthesis
BioCyc:ARA_PWY-4702 phytate degradation I
BioCyc:MTBH37RV_GLYCOLYSIS glycolysis I (from glucose 6-phosphate)
BioCyc:MOUSE_PWY-6363 D-myo-inositol (1,4,5)-trisphosphate degradation
BioCyc:ARA_PWY-882 L-ascorbate biosynthesis I (L-galactose pathway)
BioCyc:ECOL199310_PWY-2301 myo-inositol biosynthesis
PlantCyc:ARA_PWY-882 L-ascorbate biosynthesis I (L-galactose pathway)
PlantCyc:ARA_PWY-4702 phytate degradation I
PlantCyc:PLANT_PWY-2301 myo-inositol biosynthesis
PlantCyc:PLANT_PWY-882 L-ascorbate biosynthesis I (L-galactose pathway)
PlantCyc:PLANT_PWY-4702 phytate degradation I
PlantCyc:ARA_PWY-2301 myo-inositol biosynthesis
PlantCyc:ARA_HISTSYN-PWY L-histidine biosynthesis
PlantCyc:PLANT_HISTSYN-PWY L-histidine biosynthesis
Reactome:R-DRE-156584 Cytosolic sulfonation of small molecules
Reactome:R-DME-156584 Cytosolic sulfonation of small molecules
Reactome:R-MTU-870392 Mycobacterium tuberculosis biological processes
Reactome:R-XTR-156584 Cytosolic sulfonation of small molecules
Reactome:R-DME-1430728 Metabolism
Reactome:R-MTU-936635 Sulfate assimilation
Reactome:R-DRE-1430728 Metabolism
Reactome:R-DRE-156580 Phase II - Conjugation of compounds
Reactome:R-DME-156580 Phase II - Conjugation of compounds
Reactome:R-XTR-1430728 Metabolism
Reactome:R-XTR-156580 Phase II - Conjugation of compounds
Reactome:R-DRE-211859 Biological oxidations
Reactome:R-DME-211859 Biological oxidations
Reactome:R-MTU-936621 Sulfur compound metabolism
Reactome:R-XTR-211859 Biological oxidations
PathBank:SMP0002397 Inositol Metabolism
PathBank:SMP0002429 Histidine Metabolism
PathBank:SMP0000011 Inositol Metabolism
PathBank:SMP0000462 Inositol Phosphate Metabolism
PathBank:SMP0002394 Inositol Phosphate Metabolism
PathBank:SMP0087349 Inositol Phosphate Metabolism
PathBank:SMP0087389 Sulfate/Sulfite Metabolism
PathBank:SMP0012446 Ascorbate Metabolism
PathBank:SMP0087335 Inositol Metabolism