EC: 3.1.3.2

acid phosphatase (phosphate-monoester phosphohydrolase (acid optimum))

enzyme lambda metabolic reaction experiment
uniprot:P15309 [acid phosphatase(Enzyme) wildtype]

H2O + 4_Nitrophenyl_phosphate -> Phosphate + p_Nitrophenol		 (( (Vmax * A) ) / (Km + A)) buffer: 0.75 M Acetic acid-NaOH
PH: 5
Temperature: 37
uniprot:P15309 [acid phosphatase(Enzyme) wildtype]

H2O + 4_Nitrophenyl_phosphate -> p_Nitrophenol + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.75 M Acetic acid-NaOH
PH: 5
Temperature: 37
uniprot:Q540U1 [Mg2+]

4_Nitrophenyl_phosphate + H2O -> p_Nitrophenol + Phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM sodium acetate
PH: 5.6
Temperature: 37
uniprot:Q540U1 [Mg2+]

4_Nitrophenyl_phosphate + H2O -> Phosphate + p_Nitrophenol		 (( (Vmax * C) ) / (Kd + C)) buffer: 100 mM sodium acetate
PH: 5.6
Temperature: 37
uniprot:Q540U1 [Mg2+]

4_Nitrophenyl_phosphate + H2O -> Phosphate + p_Nitrophenol		 (( (Vmax * C) ) / (Kd + C)) buffer: 100 mM sodium acetate
PH: 5.6
Temperature: 37
uniprot:Q540U1 [Mg2+]

4_Nitrophenyl_phosphate + H2O -> p_Nitrophenol + Phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM sodium acetate
PH: 5.6
Temperature: 37
uniprot:Q540U1 [Mg2+]

H2O + 4_Nitrophenyl_phosphate -> Phosphate + p_Nitrophenol		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM sodium acetate
PH: 5.6
Temperature: 37
uniprot:Q540U1 [Mg2+]

4_Nitrophenyl_phosphate + H2O -> Phosphate + p_Nitrophenol		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM sodium acetate
PH: 5.6
Temperature: 37
uniprot:Q540U1 [Mg2+]

H2O + 4_Nitrophenyl_phosphate -> p_Nitrophenol + Phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM sodium acetate
PH: 5.6
Temperature: 37
uniprot:P15309 [acid phosphatase(Enzyme) wildtype neuraminidase treated]

H2O + 4_Nitrophenyl_phosphate -> Phosphate + p_Nitrophenol		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M citrate
PH: 5.5
Temperature: 37
uniprot:P15309 [acid phosphatase(Enzyme) wildtype]

H2O + 4_Nitrophenyl_phosphate -> Phosphate + p_Nitrophenol		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M citrate
PH: 5.5
Temperature: 37
uniprot:P0AE22 [acid phosphatase(Enzyme) wildtype AphA]

dGMP + H2O -> Deoxyguanosine + Phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM sodium acetate, 1 mM MgCl2
PH: 6
Temperature: 37
uniprot:P0AE22 [acid phosphatase(Enzyme) wildtype AphA]

dUMP + H2O -> Phosphate + 2'_Deoxyuridine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM sodium acetate, 1 mM MgCl2
PH: 6
Temperature: 37
uniprot:P0AE22 [acid phosphatase(Enzyme) wildtype AphA]

dAMP + H2O -> Phosphate + Deoxyadenosine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM sodium acetate, 1 mM MgCl2
PH: 6
Temperature: 37
uniprot:P0AE22 [acid phosphatase(Enzyme) wildtype AphA]

H2O + dCMP -> Phosphate + 2'_Deoxycytidine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM sodium acetate, 1 mM MgCl2
PH: 6
Temperature: 37
uniprot:P0AE22 [acid phosphatase(Enzyme) wildtype AphA]

Adenosine_3'_phosphate + H2O -> Adenosine + Phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM sodium acetate, 1 mM MgCl2
PH: 6
Temperature: 37
uniprot:P0AE22 [acid phosphatase(Enzyme) wildtype AphA]

Guanosine_3'_phosphate + H2O -> Phosphate + Guanosine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM sodium acetate, 1 mM MgCl2
PH: 6
Temperature: 37
uniprot:P0AE22 [acid phosphatase(Enzyme) wildtype AphA]

H2O + CMP -> Cytidine + Phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM sodium acetate, 1 mM MgCl2
PH: 6
Temperature: 37
uniprot:P0AE22 [acid phosphatase(Enzyme) wildtype AphA]

Uridine_3'_phosphate + H2O -> Uridine + Phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM sodium acetate, 1 mM MgCl2
PH: 6
Temperature: 37
uniprot:P0AE22 [acid phosphatase(Enzyme) wildtype AphA]

Cytidine_3'_phosphate + H2O -> Cytidine + Phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM sodium acetate, 1 mM MgCl2
PH: 6
Temperature: 37
uniprot:P0AE22 [acid phosphatase(Enzyme) wildtype AphA]

AMP + H2O -> Adenosine + Phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM sodium acetate, 1 mM MgCl2
PH: 6
Temperature: 37
uniprot:P0AE22 [acid phosphatase(Enzyme) wildtype AphA]

H2O + GMP -> Phosphate + Guanosine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM sodium acetate, 1 mM MgCl2
PH: 6
Temperature: 37
uniprot:P0AE22 [acid phosphatase(Enzyme) wildtype AphA]

UMP + H2O -> Phosphate + Uridine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM sodium acetate, 1 mM MgCl2
PH: 6
Temperature: 37
uniprot:P0AE22 [acid phosphatase(Enzyme) wildtype AphA]

Glucose_6_phosphate + H2O -> Glucose + Phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM sodium acetate, 1 mM MgCl2
PH: 6
Temperature: 37
uniprot:P0AE22 [acid phosphatase(Enzyme) wildtype AphA]

4_Nitrophenyl_phosphate + H2O -> p_Nitrophenol + Phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM sodium acetate, 1 mM MgCl2
PH: 6
Temperature: 37
uniprot:P0AE22 [acid phosphatase(Enzyme) wildtype AphA]

H2O + Glycerol_2_phosphate -> Glycerol + Phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM sodium acetate, 1 mM MgCl2
PH: 6
Temperature: 37
uniprot:P0AE22 [acid phosphatase(Enzyme) wildtype AphA]

H2O + 2'_Deoxyuridine_3'_phosphate -> Phosphate + 2'_Deoxyuridine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM sodium acetate, 1 mM MgCl2
PH: 6
Temperature: 37
uniprot:P0AE22 [acid phosphatase(Enzyme) wildtype AphA]

H2O + 2'_Deoxyguanosine_3'_phosphate -> Phosphate + Deoxyguanosine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM sodium acetate, 1 mM MgCl2
PH: 6
Temperature: 37
uniprot:P0AE22 [acid phosphatase(Enzyme) wildtype AphA]

2'_Deoxyadenosine_3'_phosphate + H2O -> Deoxyadenosine + Phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM sodium acetate, 1 mM MgCl2
PH: 6
Temperature: 37
uniprot:P0AE22 [acid phosphatase(Enzyme) wildtype AphA]

H2O + Ribose_5_phosphate -> Phosphate + Ribose		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM sodium acetate, 1 mM MgCl2
PH: 6
Temperature: 37
uniprot:P0AE22 [acid phosphatase(Enzyme) wildtype AphA]

H2O + 2'_Deoxycytidine_3'_phosphate -> 2'_Deoxycytidine + Phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM sodium acetate, 1 mM MgCl2
PH: 6
Temperature: 37
uniprot:P26976 [acid phosphatase(Enzyme) wildtype NAP I]

UMP + H2O -> Phosphate + Uridine		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris maleic acid, 1 mM MgCl2
PH: 5.5
Temperature: 37
uniprot:P15309 [acid phosphatase(Enzyme) wildtype isoenzyme PAP-II]

H2O + alpha_Naphthyl_phosphate -> alpha_Naphthol + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: -
PH: 5.5
Temperature: 37
uniprot:P15309 [acid phosphatase(Enzyme) wildtype isoenzyme PAP-I]

H2O + alpha_Naphthyl_phosphate -> alpha_Naphthol + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: -
PH: 5.5
Temperature: 37
uniprot:P15309 [acid phosphatase(Enzyme) wildtype isoenzyme PAP-I]

H2O + 4_Nitrophenyl_phosphate -> Phosphate + p_Nitrophenol		 (( (Vmax * A) ) / (Km + A)) buffer: -
PH: 5.5
Temperature: 37
uniprot:P15309 [acid phosphatase(Enzyme) wildtype isoenzyme PAP-II]

4_Nitrophenyl_phosphate + H2O -> p_Nitrophenol + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: -
PH: 5.5
Temperature: 37
uniprot:P15309 [acid phosphatase(Enzyme) wildtype isoenzyme PAP-I]

H2O + GMP -> Guanosine + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: -
PH: 5.5
Temperature: 37
uniprot:P15309 [acid phosphatase(Enzyme) wildtype isoenzyme PAP-II]

GMP + H2O -> Guanosine + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: -
PH: 5.5
Temperature: 37
uniprot:P15309 [acid phosphatase(Enzyme) wildtype isoenzyme PAP-II]

Choline_phosphate + H2O -> Choline + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: -
PH: 5.5
Temperature: 37
uniprot:P15309 [acid phosphatase(Enzyme) wildtype isoenzyme PAP-I]

Glycerol_2_phosphate + H2O -> Glycerol + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: -
PH: 5.5
Temperature: 37
uniprot:P15309 [acid phosphatase(Enzyme) wildtype isoenzyme PAP-II]

Glycerol_2_phosphate + H2O -> Glycerol + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: -
PH: 5.5
Temperature: 37
uniprot:P15309 [acid phosphatase(Enzyme) wildtype isoenzyme PAP-I]

Thymolphthalein_monophosphate + H2O -> Phosphate + Thymolphthalein		 (( (Vmax * A) ) / (Km + A)) buffer: -
PH: 5.5
Temperature: 37
uniprot:P15309 [acid phosphatase(Enzyme) wildtype isoenzyme PAP-II]

Thymolphthalein_monophosphate + H2O -> Thymolphthalein + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: -
PH: 5.5
Temperature: 37
uniprot:P15309 [acid phosphatase(Enzyme) wildtype isoenzyme PAP-I]

Choline_phosphate + H2O -> Choline + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: -
PH: 5.5
Temperature: 37
uniprot:P13686 [acid phosphatase(Enzyme) wildtype]

ATP + H2O -> ADP + Phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M sodium acetate
PH: 5.7
Temperature: 25
uniprot:P13686 [acid phosphatase(Enzyme) wildtype]

H2O + 4_Nitrophenyl_phosphate -> Phosphate + p_Nitrophenol		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M sodium acetate
PH: 5.7
Temperature: 25
uniprot:P13686 [acid phosphatase(Enzyme) wildtype]

4_Methylumbelliferyl_phosphate + H2O -> 4_Methylumbelliferone + Phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M sodium acetate
PH: 5.7
Temperature: 25
uniprot:P13686 [acid phosphatase(Enzyme) wildtype]

ADP + H2O -> Phosphate + AMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M sodium acetate
PH: 5.7
Temperature: 25
uniprot:P13686 [acid phosphatase(Enzyme) wildtype]

GDP + H2O -> GMP + Phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M sodium acetate
PH: 5.7
Temperature: 25
uniprot:P13686 [acid phosphatase(Enzyme) wildtype]

H2O + GTP -> GDP + Phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M sodium acetate
PH: 5.7
Temperature: 25
uniprot:P13686 [acid phosphatase(Enzyme) wildtype]

H2O + UDP -> UMP + Phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M sodium acetate
PH: 5.7
Temperature: 25
uniprot:P13686 [acid phosphatase(Enzyme) wildtype]

CTP + H2O -> Phosphate + CDP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M sodium acetate
PH: 5.7
Temperature: 25
uniprot:P13686 [acid phosphatase(Enzyme) wildtype]

H2O + UTP -> Phosphate + UDP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M sodium acetate
PH: 5.7
Temperature: 25
uniprot:P13686 [acid phosphatase(Enzyme) wildtype]

dTTP + H2O -> TDP + Phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M sodium acetate
PH: 5.7
Temperature: 25
uniprot:P13686 [acid phosphatase(Enzyme) wildtype]

TDP + H2O -> Thiamine_monophosphate + Phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M sodium acetate
PH: 5.7
Temperature: 25
uniprot:P13686 [acid phosphatase(Enzyme) wildtype]

Phenolphthalein_diphosphate + H2O -> 2 Phosphate + Phenolphthalein		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M sodium acetate
PH: 5.7
Temperature: 25
uniprot:P13686 [acid phosphatase(Enzyme) wildtype]

Phosphoenolpyruvate + H2O -> Phosphate + Pyruvate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M sodium acetate
PH: 5.7
Temperature: 25
uniprot:P13686 [acid phosphatase(Enzyme) wildtype]

H2O + CDP -> CMP + Phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M sodium acetate
PH: 5.7
Temperature: 25
uniprot:P13686 [acid phosphatase(Enzyme) wildtype]

Phenolic_phosphate + H2O -> Phenol + Phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M sodium acetate
PH: 5.7
Temperature: 25
uniprot:P13686 [acid phosphatase(Enzyme) wildtype]

H2O + alpha_Naphthyl_phosphate -> Phosphate + alpha_Naphthol		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M sodium acetate
PH: 5.7
Temperature: 25
- [acid phosphatase(Enzyme) wildtype]

H2O + 4_Nitrophenyl_phosphate -> Phosphate + p_Nitrophenol		 (( (Vmax * A) ) / (A + Km)) buffer: 50 mM Na-acetate, 5 mM MnCl2, 1 mM DTT
PH: 5.8
Temperature: 45
- [acid phosphatase(Enzyme) wildtype]

H2O + Phosphoenolpyruvate -> Pyruvate + Phosphate		 (( (Vmax * A) ) / (A + Km)) buffer: 50 mM Na-acetate, 5 mM MnCl2, 1 mM DTT
PH: 5.8
Temperature: 45
- [acid phosphatase(Enzyme) wildtype]

Phosphotyrosine + H2O -> Phosphate + Tyrosine		 (( (Vmax * A) ) / (A + Km)) buffer: 50 mM Na-acetate, 5 mM MnCl2, 1 mM DTT
PH: 5.8
Temperature: 45
- [acid phosphatase(Enzyme) wildtype]

H2O + Phenolic_phosphate -> Phosphate + Phenol		 (( (Vmax * A) ) / (A + Km)) buffer: 50 mM Na-acetate, 5 mM MnCl2, 1 mM DTT
PH: 5.8
Temperature: 45
- [acid phosphatase(Enzyme) wildtype]

beta_Naphthyl_phosphate + H2O -> beta_Naphthol + Phosphate		 (( (Vmax * A) ) / (A + Km)) buffer: 50 mM Na-acetate, 5 mM MnCl2, 1 mM DTT
PH: 5.8
Temperature: 45
- [acid phosphatase(Enzyme) wildtype]

H2O + ADP -> Phosphate + AMP		 (( (Vmax * A) ) / (A + Km)) buffer: 50 mM Na-acetate, 5 mM MnCl2, 1 mM DTT
PH: 5.8
Temperature: 45
- [acid phosphatase(Enzyme) wildtype]

ATP + H2O -> ADP + Phosphate		 (( (Vmax * A) ) / (A + Km)) buffer: 50 mM Na-acetate, 5 mM MnCl2, 1 mM DTT
PH: 5.8
Temperature: 45
- [acid phosphatase(Enzyme) wildtype]

Diphosphate + H2O -> 2 Phosphate		 (( (Vmax * A) ) / (A + Km)) buffer: 50 mM Na-acetate, 5 mM MnCl2, 1 mM DTT
PH: 5.8
Temperature: 45
- [acid phosphatase(Enzyme) wildtype]

H2O + CTP -> Phosphate + CDP		 (( (Vmax * A) ) / (A + Km)) buffer: 50 mM Na-acetate, 5 mM MnCl2, 1 mM DTT
PH: 5.8
Temperature: 45
- [acid phosphatase(Enzyme) wildtype]

H2O + UTP -> Phosphate + UDP		 (( (Vmax * A) ) / (A + Km)) buffer: 50 mM Na-acetate, 5 mM MnCl2, 1 mM DTT
PH: 5.8
Temperature: 45
- [acid phosphatase(Enzyme) wildtype]

H2O + Tetrapolyphosphate -> Triphosphate + Phosphate		 (( (Vmax * A) ) / (A + Km)) buffer: 50 mM Na-acetate, 5 mM MnCl2, 1 mM DTT
PH: 5.8
Temperature: 45
uniprot:P83380 [acid phosphatase(Enzyme) wildtype SAP1]

4_Nitrophenyl_phosphate + H2O -> p_Nitrophenol + Phosphate		 (( (Vmax * A) ) / (A + Km)) buffer: 50 mM Na-acetate, 5 mM MgCl2
PH: 5.3
Temperature: 40
uniprot:P83380 [acid phosphatase(Enzyme) wildtype SAP1]

ATP + H2O -> ADP + Phosphate		 (( (Vmax * A) ) / (A + Km)) buffer: 50 mM Na-acetate, 5 mM MgCl2
PH: 5.3
Temperature: 40
uniprot:P83379 [acid phosphatase(Enzyme) wildtype SAP2]

ATP + H2O -> Phosphate + ADP		 (( (Vmax * A) ) / (A + Km)) buffer: 50 mM Na-acetate, 5 mM MgCl2
PH: 5.3
Temperature: 40
uniprot:P83379 [acid phosphatase(Enzyme) wildtype SAP2]

4_Nitrophenyl_phosphate + H2O -> Phosphate + p_Nitrophenol		 (( (Vmax * A) ) / (A + Km)) buffer: 50 mM Na-acetate, 5 mM MgCl2
PH: 5.3
Temperature: 40
uniprot:P83379 [acid phosphatase(Enzyme) wildtype SAP2]

H2O + Phosphoenolpyruvate -> Phosphate + Pyruvate		 (( (Vmax * A) ) / (A + Km)) buffer: 50 mM Na-acetate, 5 mM MgCl2
PH: 5.3
Temperature: 40
uniprot:P83380 [acid phosphatase(Enzyme) wildtype SAP1]

Phosphoenolpyruvate + H2O -> Pyruvate + Phosphate		 (( (Vmax * A) ) / (A + Km)) buffer: 50 mM Na-acetate, 5 mM MgCl2
PH: 5.3
Temperature: 40
uniprot:P83380 [acid phosphatase(Enzyme) wildtype SAP1]

H2O + beta_Naphthyl_phosphate -> beta_Naphthol + Phosphate		 (( (Vmax * A) ) / (A + Km)) buffer: 50 mM Na-acetate, 5 mM MgCl2
PH: 5.3
Temperature: 40
uniprot:P83379 [acid phosphatase(Enzyme) wildtype SAP2]

beta_Naphthyl_phosphate + H2O -> beta_Naphthol + Phosphate		 (( (Vmax * A) ) / (A + Km)) buffer: 50 mM Na-acetate, 5 mM MgCl2
PH: 5.3
Temperature: 40
uniprot:P83379 [acid phosphatase(Enzyme) wildtype SAP2]

H2O + alpha_Naphthyl_phosphate -> Phosphate + alpha_Naphthol		 (( (Vmax * A) ) / (A + Km)) buffer: 50 mM Na-acetate, 5 mM MgCl2
PH: 5.3
Temperature: 40
uniprot:P83380 [acid phosphatase(Enzyme) wildtype SAP1]

alpha_Naphthyl_phosphate + H2O -> Phosphate + alpha_Naphthol		 (( (Vmax * A) ) / (A + Km)) buffer: 50 mM Na-acetate, 5 mM MgCl2
PH: 5.3
Temperature: 40
uniprot:P83379 [acid phosphatase(Enzyme) wildtype SAP2]

Diphosphate + H2O -> 2 Phosphate		 (( (Vmax * A) ) / (A + Km)) buffer: 50 mM Na-acetate, 5 mM MgCl2
PH: 5.3
Temperature: 40
uniprot:P83380 [acid phosphatase(Enzyme) wildtype SAP1]

GTP + H2O -> Phosphate + GDP		 (( (Vmax * A) ) / (A + Km)) buffer: 50 mM Na-acetate, 5 mM MgCl2
PH: 5.3
Temperature: 40
uniprot:P83379 [acid phosphatase(Enzyme) wildtype SAP2]

GTP + H2O -> Phosphate + GDP		 (( (Vmax * A) ) / (A + Km)) buffer: 50 mM Na-acetate, 5 mM MgCl2
PH: 5.3
Temperature: 40
uniprot:P83379 [acid phosphatase(Enzyme) wildtype SAP2]

H2O + Phosphotyrosine -> Phosphate + Tyrosine		 (( (Vmax * A) ) / (A + Km)) buffer: 50 mM Na-acetate, 5 mM MgCl2
PH: 5.3
Temperature: 40
uniprot:P83379 [acid phosphatase(Enzyme) wildtype SAP2]

Tetrapolyphosphate + H2O -> Triphosphate + Phosphate		 (( (Vmax * A) ) / (A + Km)) buffer: 50 mM Na-acetate, 5 mM MgCl2
PH: 5.3
Temperature: 40
uniprot:P83380 [acid phosphatase(Enzyme) wildtype SAP1]

Tetrapolyphosphate + H2O -> Triphosphate + Phosphate		 (( (Vmax * A) ) / (A + Km)) buffer: 50 mM Na-acetate, 5 mM MgCl2
PH: 5.3
Temperature: 40
uniprot:P83379 [acid phosphatase(Enzyme) wildtype SAP2]

Phenolic_phosphate + H2O -> Phosphate + Phenol		 (( (Vmax * A) ) / (A + Km)) buffer: 50 mM Na-acetate, 5 mM MgCl2
PH: 5.3
Temperature: 40
uniprot:P83380 [acid phosphatase(Enzyme) wildtype SAP1]

H2O + Phenolic_phosphate -> Phosphate + Phenol		 (( (Vmax * A) ) / (A + Km)) buffer: 50 mM Na-acetate, 5 mM MgCl2
PH: 5.3
Temperature: 40
uniprot:P83379 [acid phosphatase(Enzyme) wildtype SAP2]

6_Phosphogluconate + H2O -> Phosphate + Gluconate		 (( (Vmax * A) ) / (A + Km)) buffer: 50 mM Na-acetate, 5 mM MgCl2
PH: 5.3
Temperature: 40
uniprot:P83379 [acid phosphatase(Enzyme) wildtype SAP2]

Glycerate_3_phosphate + H2O -> Phosphate + D_Glycerate		 (( (Vmax * A) ) / (A + Km)) buffer: 50 mM Na-acetate, 5 mM MgCl2
PH: 5.3
Temperature: 40
uniprot:P83379 [acid phosphatase(Enzyme) wildtype SAP2]

H2O + sn_Glycerol_3_phosphate -> Phosphate + Glycerol		 (( (Vmax * A) ) / (A + Km)) buffer: 50 mM Na-acetate, 5 mM MgCl2
PH: 5.3
Temperature: 40
- [Mg2+]

H2O + 2_Deoxy_D_glucose_6_phosphate -> Phosphate + 2_Deoxy_D_glucose		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM 2-(N-morpholino)-ethanesulfonic acid (MES)
PH: 6
Temperature: 37
uniprot:Q84MD8 [Mg2+]

H2O + Riboflavin_5_phosphate -> Riboflavin + Phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 1 mM Dithiothreitol, 0.02% Tween 20
PH: 7.5
Temperature: 30
uniprot:Q84MD8 [Mg2+]

H2O + Riboflavin_5_phosphate -> Phosphate + Riboflavin		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 1 mM Dithiothreitol, 0.02% Tween 20
PH: 7.5
Temperature: 30
- [acid phosphatase(Enzyme) wildtype]

H2O + 4_Nitrophenyl_phosphate -> Phosphate + p_Nitrophenol		 (( (Vmax * A) ) / (A + Km)) buffer: 0.1 M NaOAc, 0.1 M NaCl, 0.2 mg/ml BSA
PH: 5.4
Temperature: 30
- [acid phosphatase(Enzyme) wildtype]

O_Phospho_L_serine + H2O -> Phosphate + Serine		 (( (Vmax * A) ) / (A + Km)) buffer: 0.1 M NaOAc, 0.1 M NaCl, 0.2 mg/ml BSA
PH: 5.4
Temperature: 30
- [acid phosphatase(Enzyme) wildtype]

H2O + D_Glucose_6_phosphate -> Phosphate + D_Glucose		 (( (Vmax * A) ) / (A + Km)) buffer: 0.1 M NaOAc, 0.1 M NaCl, 0.2 mg/ml BSA
PH: 5.4
Temperature: 30
- [acid phosphatase(Enzyme) wildtype]

Glycerate_3_phosphate + H2O -> D_Glycerate + Phosphate		 (( (Vmax * A) ) / (A + Km)) buffer: 0.1 M NaOAc, 0.1 M NaCl, 0.2 mg/ml BSA
PH: 5.4
Temperature: 30
- [acid phosphatase(Enzyme) wildtype]

H2O + Phosphotyrosine -> Phosphate + Tyrosine		 (( (Vmax * A) ) / (A + Km)) buffer: 0.1 M NaOAc, 0.1 M NaCl, 0.2 mg/ml BSA
PH: 5.4
Temperature: 30
- [acid phosphatase(Enzyme) wildtype]

L_Threonine_O_3_phosphate + H2O -> L_Threonine + Phosphate		 (( (Vmax * A) ) / (A + Km)) buffer: 0.1 M NaOAc, 0.1 M NaCl, 0.2 mg/ml BSA
PH: 5.4
Temperature: 30
- [acid phosphatase(Enzyme) wildtype]

H2O + Adenosine_2'_phosphate -> Phosphate + Adenosine		 (( (Vmax * A) ) / (A + Km)) buffer: 0.1 M NaOAc, 0.1 M NaCl, 0.2 mg/ml BSA
PH: 5.4
Temperature: 30
- [acid phosphatase(Enzyme) wildtype]

Ribose_5_phosphate + H2O -> Ribose + Phosphate		 (( (Vmax * A) ) / (A + Km)) buffer: 0.1 M NaOAc, 0.1 M NaCl, 0.2 mg/ml BSA
PH: 5.4
Temperature: 30
- [acid phosphatase(Enzyme) wildtype]

Fructose_6_phosphate + H2O -> Fructose + Phosphate		 (( (Vmax * A) ) / (A + Km)) buffer: 0.1 M NaOAc, 0.1 M NaCl, 0.2 mg/ml BSA
PH: 5.4
Temperature: 30
- [acid phosphatase(Enzyme) wildtype]

H2O + 1_Phospho_D_glycerate -> Phosphate + D_Glycerate		 (( (Vmax * A) ) / (A + Km)) buffer: 0.1 M NaOAc, 0.1 M NaCl, 0.2 mg/ml BSA
PH: 5.4
Temperature: 30
- [acid phosphatase(Enzyme) wildtype]

Adenosine_3'_phosphate + H2O -> Adenosine + Phosphate		 (( (Vmax * A) ) / (A + Km)) buffer: 0.1 M NaOAc, 0.1 M NaCl, 0.2 mg/ml BSA
PH: 5.4
Temperature: 30
- [acid phosphatase(Enzyme) wildtype]

Phosphoenolpyruvate + H2O -> Phosphate + Pyruvate		 (( (Vmax * A) ) / (A + Km)) buffer: 0.1 M NaOAc, 0.1 M NaCl, 0.2 mg/ml BSA
PH: 5.4
Temperature: 30
- [acid phosphatase(Enzyme) wildtype]

H2O + Uridine_3'_phosphate -> Uridine + Phosphate		 (( (Vmax * A) ) / (A + Km)) buffer: 0.1 M NaOAc, 0.1 M NaCl, 0.2 mg/ml BSA
PH: 5.4
Temperature: 30
- [acid phosphatase(Enzyme) wildtype]

NADPH + H2O -> NADH + Phosphate		 (( (Vmax * A) ) / (A + Km)) buffer: 0.1 M NaOAc, 0.1 M NaCl, 0.2 mg/ml BSA
PH: 5.4
Temperature: 30
- [acid phosphatase(Enzyme) wildtype]

2_Phospho_D_glycerate + H2O -> D_Glycerate + Phosphate		 (( (Vmax * A) ) / (A + Km)) buffer: 0.1 M NaOAc, 0.1 M NaCl, 0.2 mg/ml BSA
PH: 5.4
Temperature: 30
- [acid phosphatase(Enzyme) wildtype]

D_Fructose_1,6_bisphosphate + H2O -> Phosphate + D_Fructose_monophosphate		 (( (Vmax * A) ) / (A + Km)) buffer: 0.1 M NaOAc, 0.1 M NaCl, 0.2 mg/ml BSA
PH: 5.4
Temperature: 30
- [acid phosphatase(Enzyme) wildtype]

H2O + Phenolic_phosphate -> Phosphate + Phenol		 (( (Vmax * A) ) / (A + Km)) buffer: 0.1 M NaOAc, 0.1 M NaCl, 0.2 mg/ml BSA
PH: 5.4
Temperature: 30
- [acid phosphatase(Enzyme) wildtype]

o_Carboxyphenyl_phosphate + H2O -> Salicylate + Phosphate		 (( (Vmax * A) ) / (A + Km)) buffer: 0.1 M NaOAc, 0.1 M NaCl, 0.2 mg/ml BSA
PH: 5.4
Temperature: 30
- [acid phosphatase(Enzyme) wildtype]

H2O + alpha_Naphthyl_phosphate -> alpha_Naphthol + Phosphate		 (( (Vmax * A) ) / (A + Km)) buffer: 0.1 M NaOAc, 0.1 M NaCl, 0.2 mg/ml BSA
PH: 5.4
Temperature: 30
- [Phosphate]

4_Nitrophenyl_phosphate + H2O -> p_Nitrophenol + Phosphate		 (1 / (( (( (( (Km / Vmax) ) * (1 / A)) ) * (1 + (I / Ki))) ) + (1 / Vmax))) buffer: 0.1 M NaOAc, 0.1 M NaCl, 0.2 mg/ml BSA
PH: 5.4
Temperature: 30
- [acid phosphatase(Enzyme) wildtype]

H2O + ADP -> Phosphate + AMP		 (( (Vmax * A) ) / (A + Km)) buffer: 0.1 M NaOAc, 0.1 M NaCl, 0.2 mg/ml BSA
PH: 5.4
Temperature: 30
- [acid phosphatase(Enzyme) wildtype]

ATP + H2O -> Phosphate + ADP		 (( (Vmax * A) ) / (A + Km)) buffer: 0.1 M NaOAc, 0.1 M NaCl, 0.2 mg/ml BSA
PH: 5.4
Temperature: 30
- [acid phosphatase(Enzyme) wildtype]

D_Glucose_1_phosphate + H2O -> Phosphate + D_Glucose		 (( (Vmax * A) ) / (A + Km)) buffer: 0.1 M NaOAc, 0.1 M NaCl, 0.2 mg/ml BSA
PH: 5.4
Temperature: 30

Pathways

pathway id name
BioCyc:META_PWY-6348 phosphate acquisition
BioCyc:YEAST_PWY-7356 thiamin salvage IV
BioCyc:MTBH37RV_NAD-BIOSYNTHESIS-II NAD salvage pathway III (to nicotinamide riboside)
BioCyc:MTBH37RV_NADPHOS-DEPHOS-PWY NAD phosphorylation and dephosphorylation
BioCyc:MOUSE_PWY-6355 phosphate acquisition II
BioCyc:ECOL316407_SULFATE-CYS-PWY superpathway of sulfate assimilation and cysteine biosynthesis
BioCyc:YEAST_PWY-6348 phosphate acquisition
BioCyc:YEAST_PWY-6908 thiamin diphosphate biosynthesis IV (eukaryotes)
BioCyc:ARA_PWY-6348 phosphate acquisition
BioCyc:PLASMO_NAD-BIOSYNTHESIS-II NAD salvage pathway II
BioCyc:MTBH37RV_PWY-6348 phosphate acquisition
BioCyc:MTBH37RV_GLUCONEO-PWY gluconeogenesis I
BioCyc:MOUSE_NADPHOS-DEPHOS-PWY NAD phosphorylation and dephosphorylation
BioCyc:MOUSE_PWY-6348 phosphate acquisition
BioCyc:MTBH37RV_GLYCOLYSIS glycolysis I (from glucose 6-phosphate)
BioCyc:YEAST_THISYNARA-PWY-1 superpathway of thiamin diphosphate biosynthesis III (eukaryotes)
BioCyc:PLASMO_PWY-5083 NAD/NADH phosphorylation and dephosphorylation
BioCyc:MTBH37RV_PWY-6580 phosphatidylinositol biosynthesis I (bacteria)
BioCyc:MOUSE_NAD-BIOSYNTHESIS-II NAD salvage pathway II
BioCyc:ECOL316407_SERSYN-PWY serine biosynthesis
BioCyc:ECOL316407_SER-GLYSYN-PWY superpathway of serine and glycine biosynthesis I
PathBank:SMP0002103 Adenosine Nucleotides Degradation
PlantCyc:TOMATO_PWY-6348 phosphate acquisition
PlantCyc:PLANT_PWY-6348 phosphate acquisition
PlantCyc:ARA_PWY66-399 gluconeogenesis III
PlantCyc:PLANT_PWY-8011 L-serine biosynthesis II
PlantCyc:ARA_PWY-8011 L-serine biosynthesis II
PlantCyc:ARA_PWY-6348 phosphate acquisition
Reactome:R-HSA-9635486 Infection with Mycobacterium tuberculosis
Reactome:R-HSA-9637687 Suppression of phagosomal maturation
Reactome:R-HSA-9637690 Response of Mtb to phagocytosis
Reactome:R-HSA-9636569 Suppression of autophagy
Reactome:R-HSA-9636383 Prevention of phagosomal-lysosomal fusion
Reactome:R-HSA-9824439 Bacterial Infection Pathways
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-556833 Metabolism of lipids
Reactome:R-HSA-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-HSA-1643685 Disease
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-556833 Metabolism of lipids
Reactome:R-MMU-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-PFA-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-RNO-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-SPO-1430728 Metabolism
Reactome:R-SPO-1483206 Glycerophospholipid biosynthesis
Reactome:R-BTA-1483166 Synthesis of PA
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-HSA-1483166 Synthesis of PA
Reactome:R-HSA-196854 Metabolism of vitamins and cofactors
Reactome:R-HSA-196843 Vitamin B2 (riboflavin) metabolism
Reactome:R-MMU-1483166 Synthesis of PA
Reactome:R-MMU-196854 Metabolism of vitamins and cofactors
Reactome:R-MMU-196843 Vitamin B2 (riboflavin) metabolism
Reactome:R-PFA-196854 Metabolism of vitamins and cofactors
Reactome:R-RNO-196854 Metabolism of vitamins and cofactors
Reactome:R-RNO-196843 Vitamin B2 (riboflavin) metabolism
Reactome:R-SPO-1483257 Phospholipid metabolism
Reactome:R-BTA-1483257 Phospholipid metabolism
Reactome:R-HSA-1483257 Phospholipid metabolism
Reactome:R-MMU-1483257 Phospholipid metabolism
Reactome:R-PFA-1430728 Metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-SPO-1483166 Synthesis of PA
Reactome:R-BTA-1483206 Glycerophospholipid biosynthesis
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-1483206 Glycerophospholipid biosynthesis
Reactome:R-HSA-5663205 Infectious disease
Reactome:R-MMU-1483206 Glycerophospholipid biosynthesis
Reactome:R-PFA-196843 Vitamin B2 (riboflavin) metabolism
Reactome:R-SPO-556833 Metabolism of lipids
PathBank:SMP0000070 Riboflavin Metabolism
PathBank:SMP0002385 Vitamin B1/Thiamine Metabolism
PathBank:SMP0087211 Riboflavin Metabolism