EC: 3.1.1.73

feruloyl esterase (4-hydroxy-3-methoxycinnamoyl-sugar hydrolase)

enzyme lambda metabolic reaction experiment
uniprot:Q70Y21 [feruloyl esterase(Enzyme) wildtype]

H2O + Methyl_ferulate -> Methanol + Ferulate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM MOPS
PH: 6
Temperature: 37
uniprot:Q70Y21 [feruloyl esterase(Enzyme) wildtype]

H2O + Methyl_p_coumarate -> Methanol + 4_Coumarate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM MOPS
PH: 6
Temperature: 37
uniprot:Q70Y21 [feruloyl esterase(Enzyme) wildtype]

H2O + Methyl_sinapate -> Methanol + Sinapate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM MOPS
PH: 6
Temperature: 37
uniprot:Q70Y21 [feruloyl esterase(Enzyme) wildtype]

Methyl_caffeate + H2O -> Methanol + Caffeate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM MOPS
PH: 6
Temperature: 37
uniprot:Q9P979 [feruloyl esterase(Enzyme) wildtype]

H2O + 1_Naphthylbutanoic_acid -> Butanoate + alpha_Naphthol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 0.05 M sodium acetate
PH: 5
Temperature: 37
uniprot:Q9P979 [feruloyl esterase(Enzyme) wildtype]

alpha_Naphthyl_caprylate + H2O -> Octanoate + alpha_Naphthol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 0.05 M sodium acetate
PH: 5
Temperature: 37
uniprot:Q9P979 [feruloyl esterase(Enzyme) mutant T72R]

alpha_Naphthyl_caprylate + H2O -> Octanoate + alpha_Naphthol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 0.05 M sodium acetate
PH: 5
Temperature: 37
uniprot:Q9P979 [feruloyl esterase(Enzyme) mutant D71I]

alpha_Naphthyl_caprylate + H2O -> Octanoate + alpha_Naphthol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 0.05 M sodium acetate
PH: 5
Temperature: 37
uniprot:Q9P979 [feruloyl esterase(Enzyme) mutant Y80F]

alpha_Naphthyl_caprylate + H2O -> Octanoate + alpha_Naphthol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 0.05 M sodium phosphate
PH: 6
Temperature: 37
uniprot:Q9P979 [feruloyl esterase(Enzyme) mutant D77I]

1_Naphthylbutanoic_acid + H2O -> alpha_Naphthol + Butanoate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 0.05 M sodium phosphate
PH: 7.5
Temperature: 37
uniprot:Q9P979 [feruloyl esterase(Enzyme) mutant D71I]

1_Naphthylbutanoic_acid + H2O -> Butanoate + alpha_Naphthol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 0.05 M sodium acetate
PH: 5
Temperature: 37
uniprot:Q9P979 [feruloyl esterase(Enzyme) mutant D77N]

1_Naphthylbutanoic_acid + H2O -> Butanoate + alpha_Naphthol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 0.05 M sodium phosphate
PH: 7
Temperature: 37
uniprot:Q9P979 [feruloyl esterase(Enzyme) mutant Y80F]

H2O + 1_Naphthylbutanoic_acid -> Butanoate + alpha_Naphthol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 0.05 M sodium phosphate
PH: 6
Temperature: 37
uniprot:Q9P979 [feruloyl esterase(Enzyme) mutant T72R]

1_Naphthylbutanoic_acid + H2O -> alpha_Naphthol + Butanoate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 0.05 M sodium acetate
PH: 5
Temperature: 37

Pathways

pathway id name