EC: 2.7.7.7

DNA-directed DNA polymerase (deoxynucleoside-triphosphate:DNA deoxynucleotidyltransferase (DNA-directed))

enzyme lambda metabolic reaction experiment
uniprot:P04415 [DNA-directed DNA polymerase(Enzyme) wildtype]

H2O + DNA -> dTMP + DNA		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 66 mM Tris, 17 mM (NH4)2SO4, 10 mM 2-mercaptoethanol, 6.5 mM MgSO4, 166 µg/ml bovine serum albumin
PH: 8.8
Temperature: 30
uniprot:P04415 [DNA-directed DNA polymerase(Enzyme) wildtype residues 1-388]

H2O + DNA -> dTMP + DNA		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 66 mM Tris, 17 mM (NH4)2SO4, 10 mM 2-mercaptoethanol, 6.5 mM MgSO4, 166 µg/ml bovine serum albumin
PH: 8.8
Temperature: 30
uniprot:P04415 [DNA-directed DNA polymerase(Enzyme) wildtype residues 1-698]

H2O + DNA -> DNA + dTMP		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 66 mM Tris, 17 mM (NH4)2SO4, 10 mM 2-mercaptoethanol, 6.5 mM MgSO4, 166 µg/ml bovine serum albumin
PH: 8.8
Temperature: 30
uniprot:P04415 [DNA-directed DNA polymerase(Enzyme) wildtype]

H2O + DNA -> DNA + dTMP		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 66 mM Tris, 17 mM (NH4)2SO4, 10 mM 2-mercaptoethanol, 6.5 mM MgSO4, 166 µg/ml bovine serum albumin
PH: 8.8
Temperature: 30
uniprot:P04415 [DNA-directed DNA polymerase(Enzyme) wildtype residues 1-698]

DNA + H2O -> DNA + dTMP		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 66 mM Tris, 17 mM (NH4)2SO4, 10 mM 2-mercaptoethanol, 6.5 mM MgSO4, 166 µg/ml bovine serum albumin
PH: 8.8
Temperature: 30
uniprot:P00582 [DNA-directed DNA polymerase(Enzyme) wildtype Klenow]

DNA + H2O -> dTMP + DNA		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 66 mM Tris, 17 mM (NH4)2SO4, 10 mM 2-mercaptoethanol, 6.5 mM MgSO4, 166 µg/ml bovine serum albumin
PH: 8.8
Temperature: 30
uniprot:P00582 [DNA-directed DNA polymerase(Enzyme) wildtype Klenow]

H2O + DNA -> DNA + dTMP		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 66 mM Tris, 17 mM (NH4)2SO4, 10 mM 2-mercaptoethanol, 6.5 mM MgSO4, 166 µg/ml bovine serum albumin
PH: 8.8
Temperature: 30
- [DNA-directed DNA polymerase(Enzyme) wildtype GST-Rev3-Rev7]

dTTP + DNA -> Diphosphate + DNA		 (( (Vmax * S) ) / (Km + S)) buffer: 40 mM Tris-HCl, 5 mM MgCl2, 1 mM dithiothreitol, 100 μg/ml BSA, 10 % glycerol, 20 nM 5'-32P-labeled oligonucleotide primer annealed to an oligonucleotide template
PH: 7.5
Temperature: 30
- [DNA-directed DNA polymerase(Enzyme) wildtype GST-Rev3-Rev7]

DNA + dTTP -> DNA + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 40 mM Tris-HCl, 5 mM MgCl2, 1 mM dithiothreitol, 100 μg/ml BSA, 10 % glycerol, 20 nM 5'-32P-labeled oligonucleotide primer annealed to an oligonucleotide template
PH: 7.5
Temperature: 30
- [DNA-directed DNA polymerase(Enzyme) wildtype GST-Rev3-Rev7]

dTTP + DNA -> DNA + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 40 mM Tris-HCl, 5 mM MgCl2, 1 mM dithiothreitol, 100 μg/ml BSA, 10 % glycerol, 20 nM 5'-32P-labeled oligonucleotide primer annealed to an oligonucleotide template
PH: 7.5
Temperature: 30
- [DNA-directed DNA polymerase(Enzyme) wildtype GST-Rev3-Rev7]

DNA + dATP -> Diphosphate + DNA		 (( (Vmax * S) ) / (Km + S)) buffer: 40 mM Tris-HCl, 5 mM MgCl2, 1 mM dithiothreitol, 100 μg/ml BSA, 10 % glycerol, 20 nM 5'-32P-labeled oligonucleotide primer annealed to an oligonucleotide template
PH: 7.5
Temperature: 30
- [DNA-directed DNA polymerase(Enzyme) wildtype GST-Rev3-Rev7]

DNA + dGTP -> DNA + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 40 mM Tris-HCl, 5 mM MgCl2, 1 mM dithiothreitol, 100 μg/ml BSA, 10 % glycerol, 20 nM 5'-32P-labeled oligonucleotide primer annealed to an oligonucleotide template
PH: 7.5
Temperature: 30
- [DNA-directed DNA polymerase(Enzyme) wildtype GST-Rev3-Rev7]

DNA + dTTP -> DNA + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 40 mM Tris-HCl, 5 mM MgCl2, 1 mM dithiothreitol, 100 μg/ml BSA, 10 % glycerol, 20 nM 5'-32P-labeled oligonucleotide primer annealed to an oligonucleotide template
PH: 7.5
Temperature: 30
- [DNA-directed DNA polymerase(Enzyme) wildtype GST-Rev3-Rev7]

dATP + DNA -> Diphosphate + DNA		 (( (Vmax * S) ) / (Km + S)) buffer: 40 mM Tris-HCl, 5 mM MgCl2, 1 mM dithiothreitol, 100 μg/ml BSA, 10 % glycerol, 20 nM 5'-32P-labeled oligonucleotide primer annealed to an oligonucleotide template
PH: 7.5
Temperature: 30
- [DNA-directed DNA polymerase(Enzyme) wildtype GST-Rev3-Rev7]

DNA + dCTP -> Diphosphate + DNA		 (( (Vmax * S) ) / (Km + S)) buffer: 40 mM Tris-HCl, 5 mM MgCl2, 1 mM dithiothreitol, 100 μg/ml BSA, 10 % glycerol, 20 nM 5'-32P-labeled oligonucleotide primer annealed to an oligonucleotide template
PH: 7.5
Temperature: 30
- [DNA-directed DNA polymerase(Enzyme) wildtype GST-Rev3-Rev7]

dGTP + DNA -> DNA + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 40 mM Tris-HCl, 5 mM MgCl2, 1 mM dithiothreitol, 100 μg/ml BSA, 10 % glycerol, 20 nM 5'-32P-labeled oligonucleotide primer annealed to an oligonucleotide template
PH: 7.5
Temperature: 30
- [DNA-directed DNA polymerase(Enzyme) wildtype GST-Rev3-Rev7]

DNA + dTTP -> Diphosphate + DNA		 (( (Vmax * S) ) / (Km + S)) buffer: 40 mM Tris-HCl, 5 mM MgCl2, 1 mM dithiothreitol, 100 μg/ml BSA, 10 % glycerol, 20 nM 5'-32P-labeled oligonucleotide primer annealed to an oligonucleotide template
PH: 7.5
Temperature: 30
uniprot:O75417 [DNA-directed DNA polymerase(Enzyme) wildtype POLQ (aa 1792-2590)]

DNA + dATP -> Diphosphate + DNA		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Tris-HCl, 4% glycerol, 80 mg/ml bovine serum albumin, 0.1 mM EDTA, 10 mM MgCl2
PH: 8.8
Temperature: 36
uniprot:O75417 [DNA-directed DNA polymerase(Enzyme) wildtype POLQ (aa 1792-2590)]

DNA + dATP -> Diphosphate + DNA		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Tris-HCl, 4% glycerol, 80 mg/ml bovine serum albumin, 0.1 mM EDTA, 10 mM MgCl2
PH: 8.8
Temperature: 36
uniprot:O75417 [DNA-directed DNA polymerase(Enzyme) wildtype POLQ (aa 1792-2590)]

Single_stranded_DNA + dGTP -> Single_stranded_DNA + Diphosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Tris-HCl, 4% glycerol, 80 mg/ml bovine serum albumin, 0.1 mM EDTA, 10 mM MgCl2
PH: 8.8
Temperature: 36
- [DNA-directed DNA polymerase(Enzyme) wildtype GST-Rev3-Rev7]

dTTP + DNA -> DNA + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 40 mM Tris-HCl, 5 mM MgCl2, 1 mM dithiothreitol, 100 μg/ml BSA, 10 % glycerol, 20 nM 5'-32P-labeled oligonucleotide primer annealed to an oligonucleotide template
PH: 7.5
Temperature: 30
- [DNA-directed DNA polymerase(Enzyme) wildtype GST-Rev3-Rev7]

dTTP + DNA -> DNA + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 40 mM Tris-HCl, 5 mM MgCl2, 1 mM dithiothreitol, 100 μg/ml BSA, 10 % glycerol, 20 nM 5'-32P-labeled oligonucleotide primer annealed to an oligonucleotide template
PH: 7.5
Temperature: 30
- [DNA-directed DNA polymerase(Enzyme) wildtype GST-Rev3-Rev7]

dATP + DNA -> Diphosphate + DNA		 (( (Vmax * S) ) / (Km + S)) buffer: 40 mM Tris-HCl, 5 mM MgCl2, 1 mM dithiothreitol, 100 μg/ml BSA, 10 % glycerol, 20 nM 5'-32P-labeled oligonucleotide primer annealed to an oligonucleotide template
PH: 7.5
Temperature: 30
- [DNA-directed DNA polymerase(Enzyme) wildtype GST-Rev3-Rev7]

DNA + dGTP -> Diphosphate + DNA		 (( (Vmax * S) ) / (Km + S)) buffer: 40 mM Tris-HCl, 5 mM MgCl2, 1 mM dithiothreitol, 100 μg/ml BSA, 10 % glycerol, 20 nM 5'-32P-labeled oligonucleotide primer annealed to an oligonucleotide template
PH: 7.5
Temperature: 30
- [DNA-directed DNA polymerase(Enzyme) wildtype GST-Rev3-Rev7]

dCTP + DNA -> Diphosphate + DNA		 (( (Vmax * S) ) / (Km + S)) buffer: 40 mM Tris-HCl, 5 mM MgCl2, 1 mM dithiothreitol, 100 μg/ml BSA, 10 % glycerol, 20 nM 5'-32P-labeled oligonucleotide primer annealed to an oligonucleotide template
PH: 7.5
Temperature: 30
- [DNA-directed DNA polymerase(Enzyme) wildtype GST-Rev3-Rev7]

DNA + dTTP -> DNA + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 40 mM Tris-HCl, 5 mM MgCl2, 1 mM dithiothreitol, 100 μg/ml BSA, 10 % glycerol, 20 nM 5'-32P-labeled oligonucleotide primer annealed to an oligonucleotide template
PH: 7.5
Temperature: 30
- [DNA-directed DNA polymerase(Enzyme) wildtype GST-Rev3-Rev7]

DNA + dTTP -> DNA + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 40 mM Tris-HCl, 5 mM MgCl2, 1 mM dithiothreitol, 100 μg/ml BSA, 10 % glycerol, 20 nM 5'-32P-labeled oligonucleotide primer annealed to an oligonucleotide template
PH: 7.5
Temperature: 30
- [DNA-directed DNA polymerase(Enzyme) wildtype GST-Rev3-Rev7]

dTTP + DNA -> Diphosphate + DNA		 (( (Vmax * S) ) / (Km + S)) buffer: 40 mM Tris-HCl, 5 mM MgCl2, 1 mM dithiothreitol, 100 μg/ml BSA, 10 % glycerol, 20 nM 5'-32P-labeled oligonucleotide primer annealed to an oligonucleotide template
PH: 7.5
Temperature: 30
- [DNA-directed DNA polymerase(Enzyme) wildtype GST-Rev3-Rev7]

DNA + dTTP -> Diphosphate + DNA		 (( (Vmax * S) ) / (Km + S)) buffer: 40 mM Tris-HCl, 5 mM MgCl2, 1 mM dithiothreitol, 100 μg/ml BSA, 10 % glycerol, 20 nM 5'-32P-labeled oligonucleotide primer annealed to an oligonucleotide template
PH: 7.5
Temperature: 30
- [DNA-directed DNA polymerase(Enzyme) wildtype GST-Rev3-Rev7]

DNA + dTTP -> Diphosphate + DNA		 (( (Vmax * S) ) / (Km + S)) buffer: 40 mM Tris-HCl, 5 mM MgCl2, 1 mM dithiothreitol, 100 μg/ml BSA, 10 % glycerol, 20 nM 5'-32P-labeled oligonucleotide primer annealed to an oligonucleotide template
PH: 7.5
Temperature: 30
- [DNA-directed DNA polymerase(Enzyme) wildtype GST-Rev3-Rev7]

DNA + dTTP -> Diphosphate + DNA		 (( (Vmax * S) ) / (Km + S)) buffer: 40 mM Tris-HCl, 5 mM MgCl2, 1 mM dithiothreitol, 100 μg/ml BSA, 10 % glycerol, 20 nM 5'-32P-labeled oligonucleotide primer annealed to an oligonucleotide template
PH: 7.5
Temperature: 30
- [DNA-directed DNA polymerase(Enzyme) wildtype GST-Rev3-Rev7]

dTTP + DNA -> DNA + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 40 mM Tris-HCl, 5 mM MgCl2, 1 mM dithiothreitol, 100 μg/ml BSA, 10 % glycerol, 20 nM 5'-32P-labeled oligonucleotide primer annealed to an oligonucleotide template
PH: 7.5
Temperature: 30
- [DNA-directed DNA polymerase(Enzyme) wildtype GST-Rev3-Rev7]

dTTP + DNA -> Diphosphate + DNA		 (( (Vmax * S) ) / (Km + S)) buffer: 40 mM Tris-HCl, 5 mM MgCl2, 1 mM dithiothreitol, 100 μg/ml BSA, 10 % glycerol, 20 nM 5'-32P-labeled oligonucleotide primer annealed to an oligonucleotide template
PH: 7.5
Temperature: 30
- [DNA-directed DNA polymerase(Enzyme) wildtype GST-Rev3-Rev7]

DNA + dCTP -> Diphosphate + DNA		 (( (Vmax * S) ) / (Km + S)) buffer: 40 mM Tris-HCl, 5 mM MgCl2, 1 mM dithiothreitol, 100 μg/ml BSA, 10 % glycerol, 20 nM 5'-32P-labeled oligonucleotide primer annealed to an oligonucleotide template
PH: 7.5
Temperature: 30
uniprot:Q38087 [DNA-directed DNA polymerase(Enzyme) mutant D222A/D327A/K486A]

dATP + DNA -> Diphosphate + DNA		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 66 mM Tris-HCl, 17 mM (NH4)2SO4, 10 mM beta-mercaptoethanol, 6.5 mM MgSO4, 10% glycerol, 166 µg/ml BSA, a 100-fold excess of dCTP
PH: 8.8
Temperature: 30
uniprot:Q38087 [DNA-directed DNA polymerase(Enzyme) mutant D222A/D327A/Y567A]

dATP + DNA -> DNA + Diphosphate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 66 mM Tris-HCl, 17 mM (NH4)2SO4, 10 mM beta-mercaptoethanol, 6.5 mM MgSO4, 10% glycerol, 166 µg/ml BSA, a 100-fold excess of dCTP
PH: 8.8
Temperature: 30
uniprot:Q38087 [DNA-directed DNA polymerase(Enzyme) mutant D222A/D327A/K483A]

DNA + dATP -> Diphosphate + DNA		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 66 mM Tris-HCl, 17 mM (NH4)2SO4, 10 mM beta-mercaptoethanol, 6.5 mM MgSO4, 10% glycerol, 166 µg/ml BSA, a 100-fold excess of dCTP
PH: 8.8
Temperature: 30
uniprot:Q38087 [DNA-directed DNA polymerase(Enzyme) mutant D222A/D327A]

DNA + dATP -> Diphosphate + DNA		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 66 mM Tris-HCl, 17 mM (NH4)2SO4, 10 mM beta-mercaptoethanol, 6.5 mM MgSO4, 10% glycerol, 166 µg/ml BSA, a 100-fold excess of dCTP
PH: 8.8
Temperature: 30
uniprot:Q38087 [DNA-directed DNA polymerase(Enzyme) mutant D222A/D327A/R482A]

dATP + DNA -> DNA + Diphosphate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 66 mM Tris-HCl, 17 mM (NH4)2SO4, 10 mM beta-mercaptoethanol, 6.5 mM MgSO4, 10% glycerol, 166 µg/ml BSA, a 100-fold excess of dCTP
PH: 8.8
Temperature: 30
uniprot:Q38087 [DNA-directed DNA polymerase(Enzyme) mutant D222A/D327A/K560A]

dATP + DNA -> Diphosphate + DNA		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 66 mM Tris-HCl, 17 mM (NH4)2SO4, 10 mM beta-mercaptoethanol, 6.5 mM MgSO4, 10% glycerol, 166 µg/ml BSA, a 100-fold excess of dCTP
PH: 8.8
Temperature: 30
uniprot:Q38087 [DNA-directed DNA polymerase(Enzyme) mutant D222A/D327A/N564A]

DNA + dATP -> Diphosphate + DNA		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 66 mM Tris-HCl, 17 mM (NH4)2SO4, 10 mM beta-mercaptoethanol, 6.5 mM MgSO4, 10% glycerol, 166 µg/ml BSA, a 100-fold excess of dCTP
PH: 8.8
Temperature: 30
uniprot:Q38087 [DNA-directed DNA polymerase(Enzyme) mutant D222A/D327A/K483A]

dGTP + DNA -> DNA + Diphosphate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 66 mM Tris-HCl, 17 mM (NH4)2SO4, 10 mM beta-mercaptoethanol, 6.5 mM MgSO4, 10% glycerol, 166 µg/ml BSA, a 100-fold excess of dTTP
PH: 8.8
Temperature: 30
uniprot:Q38087 [DNA-directed DNA polymerase(Enzyme) mutant D222A/D327A/N564A]

dCTP + DNA -> DNA + Diphosphate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 66 mM Tris-HCl, 17 mM (NH4)2SO4, 10 mM beta-mercaptoethanol, 6.5 mM MgSO4, 10% glycerol, 166 µg/ml BSA, a 100-fold excess of dATP
PH: 8.8
Temperature: 30
uniprot:Q38087 [DNA-directed DNA polymerase(Enzyme) mutant D222A/D327A]

dGTP + DNA -> Diphosphate + DNA		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 66 mM Tris-HCl, 17 mM (NH4)2SO4, 10 mM beta-mercaptoethanol, 6.5 mM MgSO4, 10% glycerol, 166 µg/ml BSA, a 100-fold excess of dTTP
PH: 8.8
Temperature: 30
uniprot:Q38087 [DNA-directed DNA polymerase(Enzyme) mutant D222A/D327A/Y567A]

dCTP + DNA -> Diphosphate + DNA		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 66 mM Tris-HCl, 17 mM (NH4)2SO4, 10 mM beta-mercaptoethanol, 6.5 mM MgSO4, 10% glycerol, 166 µg/ml BSA, a 100-fold excess of dATP
PH: 8.8
Temperature: 30
uniprot:Q38087 [DNA-directed DNA polymerase(Enzyme) mutant D222A/D327A/K486A]

DNA + dCTP -> DNA + Diphosphate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 66 mM Tris-HCl, 17 mM (NH4)2SO4, 10 mM beta-mercaptoethanol, 6.5 mM MgSO4, 10% glycerol, 166 µg/ml BSA, a 100-fold excess of dATP
PH: 8.8
Temperature: 30
uniprot:Q38087 [DNA-directed DNA polymerase(Enzyme) mutant D222A/D327A/K560A]

DNA + dCTP -> Diphosphate + DNA		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 66 mM Tris-HCl, 17 mM (NH4)2SO4, 10 mM beta-mercaptoethanol, 6.5 mM MgSO4, 10% glycerol, 166 µg/ml BSA, a 100-fold excess of dATP
PH: 8.8
Temperature: 30
uniprot:Q38087 [DNA-directed DNA polymerase(Enzyme) mutant D222A/D327A/K483A]

DNA + dCTP -> DNA + Diphosphate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 66 mM Tris-HCl, 17 mM (NH4)2SO4, 10 mM beta-mercaptoethanol, 6.5 mM MgSO4, 10% glycerol, 166 µg/ml BSA, a 100-fold excess of dATP
PH: 8.8
Temperature: 30
uniprot:Q38087 [DNA-directed DNA polymerase(Enzyme) mutant D222A/D327A/R482A]

DNA + dCTP -> Diphosphate + DNA		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 66 mM Tris-HCl, 17 mM (NH4)2SO4, 10 mM beta-mercaptoethanol, 6.5 mM MgSO4, 10% glycerol, 166 µg/ml BSA, a 100-fold excess of dATP
PH: 8.8
Temperature: 30
uniprot:Q38087 [DNA-directed DNA polymerase(Enzyme) mutant D222A/D327A]

DNA + dCTP -> DNA + Diphosphate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 66 mM Tris-HCl, 17 mM (NH4)2SO4, 10 mM beta-mercaptoethanol, 6.5 mM MgSO4, 10% glycerol, 166 µg/ml BSA, a 100-fold excess of dATP
PH: 8.8
Temperature: 30
uniprot:Q38087 [DNA-directed DNA polymerase(Enzyme) mutant D222A/D327A/Y567A]

DNA + dTTP -> Diphosphate + DNA		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 66 mM Tris-HCl, 17 mM (NH4)2SO4, 10 mM beta-mercaptoethanol, 6.5 mM MgSO4, 10% glycerol, 166 µg/ml BSA, a 100-fold excess of dGTP
PH: 8.8
Temperature: 30
uniprot:Q38087 [DNA-directed DNA polymerase(Enzyme) mutant D222A/D327A/R482A]

dTTP + DNA -> Diphosphate + DNA		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 66 mM Tris-HCl, 17 mM (NH4)2SO4, 10 mM beta-mercaptoethanol, 6.5 mM MgSO4, 10% glycerol, 166 µg/ml BSA, a 100-fold excess of dGTP
PH: 8.8
Temperature: 30
uniprot:Q38087 [DNA-directed DNA polymerase(Enzyme) mutant D222A/D327A/R482A]

dGTP + DNA -> Diphosphate + DNA		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 66 mM Tris-HCl, 17 mM (NH4)2SO4, 10 mM beta-mercaptoethanol, 6.5 mM MgSO4, 10% glycerol, 166 µg/ml BSA, a 100-fold excess of dTTP
PH: 8.8
Temperature: 30
uniprot:Q38087 [DNA-directed DNA polymerase(Enzyme) mutant D222A/D327A]

dTTP + DNA -> Diphosphate + DNA		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 66 mM Tris-HCl, 17 mM (NH4)2SO4, 10 mM beta-mercaptoethanol, 6.5 mM MgSO4, 10% glycerol, 166 µg/ml BSA, a 100-fold excess of dGTP
PH: 8.8
Temperature: 30
uniprot:Q38087 [DNA-directed DNA polymerase(Enzyme) mutant D222A/D327A/K483A]

dTTP + DNA -> DNA + Diphosphate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 66 mM Tris-HCl, 17 mM (NH4)2SO4, 10 mM beta-mercaptoethanol, 6.5 mM MgSO4, 10% glycerol, 166 µg/ml BSA, a 100-fold excess of dGTP
PH: 8.8
Temperature: 30
uniprot:Q38087 [DNA-directed DNA polymerase(Enzyme) mutant D222A/D327A/Y567A]

dGTP + DNA -> Diphosphate + DNA		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 66 mM Tris-HCl, 17 mM (NH4)2SO4, 10 mM beta-mercaptoethanol, 6.5 mM MgSO4, 10% glycerol, 166 µg/ml BSA, a 100-fold excess of dTTP
PH: 8.8
Temperature: 30
uniprot:Q38087 [DNA-directed DNA polymerase(Enzyme) mutant D222A/D327A/K486A]

DNA + dGTP -> DNA + Diphosphate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 66 mM Tris-HCl, 17 mM (NH4)2SO4, 10 mM beta-mercaptoethanol, 6.5 mM MgSO4, 10% glycerol, 166 µg/ml BSA, a 100-fold excess of dTTP
PH: 8.8
Temperature: 30
uniprot:Q38087 [DNA-directed DNA polymerase(Enzyme) mutant D222A/D327A/K560A]

DNA + dGTP -> DNA + Diphosphate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 66 mM Tris-HCl, 17 mM (NH4)2SO4, 10 mM beta-mercaptoethanol, 6.5 mM MgSO4, 10% glycerol, 166 µg/ml BSA, a 100-fold excess of dTTP
PH: 8.8
Temperature: 30
uniprot:Q38087 [DNA-directed DNA polymerase(Enzyme) mutant D222A/D327A/N564A]

dGTP + DNA -> DNA + Diphosphate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 66 mM Tris-HCl, 17 mM (NH4)2SO4, 10 mM beta-mercaptoethanol, 6.5 mM MgSO4, 10% glycerol, 166 µg/ml BSA, a 100-fold excess of dTTP
PH: 8.8
Temperature: 30
uniprot:Q38087 [DNA-directed DNA polymerase(Enzyme) mutant D222A/D327A/K486A]

dTTP + DNA -> Diphosphate + DNA		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 66 mM Tris-HCl, 17 mM (NH4)2SO4, 10 mM beta-mercaptoethanol, 6.5 mM MgSO4, 10% glycerol, 166 µg/ml BSA, a 100-fold excess of dGTP
PH: 8.8
Temperature: 30
uniprot:Q38087 [DNA-directed DNA polymerase(Enzyme) mutant D222A/D327A/N564A]

DNA + dTTP -> Diphosphate + DNA		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 66 mM Tris-HCl, 17 mM (NH4)2SO4, 10 mM beta-mercaptoethanol, 6.5 mM MgSO4, 10% glycerol, 166 µg/ml BSA, a 100-fold excess of dGTP
PH: 8.8
Temperature: 30
uniprot:Q38087 [DNA-directed DNA polymerase(Enzyme) mutant D222A/D327A/K560A]

DNA + dTTP -> DNA + Diphosphate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 66 mM Tris-HCl, 17 mM (NH4)2SO4, 10 mM beta-mercaptoethanol, 6.5 mM MgSO4, 10% glycerol, 166 µg/ml BSA, a 100-fold excess of dGTP
PH: 8.8
Temperature: 30
uniprot:Q38087 [DNA-directed DNA polymerase(Enzyme) mutant D222A/D327A/R482A]

DNA + dUTP -> DNA + Diphosphate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 66 mM Tris-HCl, 17 mM (NH4)2SO4, 10 mM beta-mercaptoethanol, 6.5 mM MgSO4, 10% glycerol, 166 µg/ml BSA, a 100-fold excess of dGTP
PH: 8.8
Temperature: 30
uniprot:Q38087 [DNA-directed DNA polymerase(Enzyme) mutant D222A/D327A/K560A]

dUTP + DNA -> DNA + Diphosphate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 66 mM Tris-HCl, 17 mM (NH4)2SO4, 10 mM beta-mercaptoethanol, 6.5 mM MgSO4, 10% glycerol, 166 µg/ml BSA, a 100-fold excess of dGTP
PH: 8.8
Temperature: 30
uniprot:Q38087 [DNA-directed DNA polymerase(Enzyme) mutant D222A/D327A/K483A]

dUTP + DNA -> Diphosphate + DNA		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 66 mM Tris-HCl, 17 mM (NH4)2SO4, 10 mM beta-mercaptoethanol, 6.5 mM MgSO4, 10% glycerol, 166 µg/ml BSA, a 100-fold excess of dGTP
PH: 8.8
Temperature: 30
uniprot:Q38087 [DNA-directed DNA polymerase(Enzyme) mutant D222A/D327A]

dUTP + DNA -> Diphosphate + DNA		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 66 mM Tris-HCl, 17 mM (NH4)2SO4, 10 mM beta-mercaptoethanol, 6.5 mM MgSO4, 10% glycerol, 166 µg/ml BSA, a 100-fold excess of dGTP
PH: 8.8
Temperature: 30
uniprot:Q38087 [DNA-directed DNA polymerase(Enzyme) mutant D222A/D327A/Y567A]

DNA + dUTP -> DNA + Diphosphate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 66 mM Tris-HCl, 17 mM (NH4)2SO4, 10 mM beta-mercaptoethanol, 6.5 mM MgSO4, 10% glycerol, 166 µg/ml BSA, a 100-fold excess of dGTP
PH: 8.8
Temperature: 30
uniprot:Q38087 [DNA-directed DNA polymerase(Enzyme) mutant D222A/D327A/K486A]

DNA + dUTP -> Diphosphate + DNA		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 66 mM Tris-HCl, 17 mM (NH4)2SO4, 10 mM beta-mercaptoethanol, 6.5 mM MgSO4, 10% glycerol, 166 µg/ml BSA, a 100-fold excess of dGTP
PH: 8.8
Temperature: 30
uniprot:Q38087 [DNA-directed DNA polymerase(Enzyme) mutant D222A/D327A/R482A]

DNA + dGDP -> DNA + Phosphate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 66 mM Tris-HCl, 17 mM (NH4)2SO4, 10 mM beta-mercaptoethanol, 6.5 mM MgSO4, 10% glycerol, 166 µg/ml BSA, a 100-fold excess of dTTP
PH: 8.8
Temperature: 30
uniprot:Q38087 [DNA-directed DNA polymerase(Enzyme) mutant D222A/D327A/N564A]

dGDP + DNA -> DNA + Phosphate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 66 mM Tris-HCl, 17 mM (NH4)2SO4, 10 mM beta-mercaptoethanol, 6.5 mM MgSO4, 10% glycerol, 166 µg/ml BSA, a 100-fold excess of dTTP
PH: 8.8
Temperature: 30
uniprot:Q38087 [DNA-directed DNA polymerase(Enzyme) mutant D222A/D327A/K486A]

DNA + dGDP -> DNA + Phosphate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 66 mM Tris-HCl, 17 mM (NH4)2SO4, 10 mM beta-mercaptoethanol, 6.5 mM MgSO4, 10% glycerol, 166 µg/ml BSA, a 100-fold excess of dTTP
PH: 8.8
Temperature: 30
uniprot:Q38087 [DNA-directed DNA polymerase(Enzyme) mutant D222A/D327A/N564A]

dUTP + DNA -> DNA + Diphosphate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 66 mM Tris-HCl, 17 mM (NH4)2SO4, 10 mM beta-mercaptoethanol, 6.5 mM MgSO4, 10% glycerol, 166 µg/ml BSA, a 100-fold excess of dGTP
PH: 8.8
Temperature: 30
uniprot:Q38087 [DNA-directed DNA polymerase(Enzyme) mutant D222A/D327A/K560A]

dGDP + DNA -> Phosphate + DNA		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 66 mM Tris-HCl, 17 mM (NH4)2SO4, 10 mM beta-mercaptoethanol, 6.5 mM MgSO4, 10% glycerol, 166 µg/ml BSA, a 100-fold excess of dTTP
PH: 8.8
Temperature: 30
uniprot:Q9UGP5 [Mn2+]

DNA + dTTP -> Diphosphate + DNA		 (Vmax / (( (( (( (1 + (Km / S)) ) + (Kd / A)) ) + (( (( (Km * Kd) ) / A) ) * S)) ) + (A / Kd_2))) buffer: 50 mM Tris-HCl, 0.25 mg/ml bovine serum albumin, 1 mM Dithiothreitol
PH: 7.6
Temperature: 37
uniprot:Q9UGP5 [Mn2+]

DNA + dTTP -> DNA + Diphosphate		 (Vmax / (( (( (( (1 + (Km / S)) ) + (Kd / A)) ) + (( (( (Km * Kd) ) / A) ) * S)) ) + (A / Kd_2))) buffer: 50 mM Tris-HCl, 0.25 mg/ml bovine serum albumin, 1 mM Dithiothreitol
PH: 7.6
Temperature: 37
uniprot:Q9UGP5 [Mg2+]

dTTP + DNA -> Diphosphate + DNA		 (Vmax / (1 + (Kd / C))) buffer: 50 mM Tris-HCl, 0.25 mg/ml bovine serum albumin, 1 mM Dithiothreitol
PH: 7.6
Temperature: 37
uniprot:Q9UGP5 [Mn2+]

dTTP + DNA -> Diphosphate + DNA		 (Vmax / (1 + (Kd / C))) buffer: 50 mM Tris-HCl, 0.25 mg/ml bovine serum albumin, 1 mM Dithiothreitol
PH: 7.6
Temperature: 37
uniprot:Q9UGP5 [Mg2+]

dTTP + DNA -> Diphosphate + DNA		 (Vmax / (1 + (Kd / C))) buffer: 50 mM Tris-HCl, 0.25 mg/ml bovine serum albumin, 1 mM Dithiothreitol
PH: 7.6
Temperature: 37
- [DNA-directed DNA polymerase(Enzyme) mutant Y391A]

dGTP + DNA -> DNA + Diphosphate		 (( (( (E * k_pol) ) * A) ) / (Kd + A)) buffer: 66 mM Tris-HCl, 10 mM MgSO4
PH: 7.5
Temperature: 25
- [DNA-directed DNA polymerase(Enzyme) wildtype]

dCTP + DNA -> Diphosphate + DNA		 (( (( (E * k_pol) ) * A) ) / (Kd + A)) buffer: 66 mM Tris-HCl, 10 mM MgSO4
PH: 7.5
Temperature: 25
- [DNA-directed DNA polymerase(Enzyme) wildtype]

dGTP + DNA -> Diphosphate + DNA		 (( (( (E * k_pol) ) * A) ) / (Kd + A)) buffer: 66 mM Tris-HCl, 10 mM MgSO4
PH: 7.5
Temperature: 25
- [DNA-directed DNA polymerase(Enzyme) mutant Y567F]

dCTP + DNA -> Diphosphate + DNA		 (( (( (E * k_pol) ) * A) ) / (Kd + A)) buffer: 66 mM Tris-HCl, 10 mM MgSO4
PH: 7.5
Temperature: 25
- [DNA-directed DNA polymerase(Enzyme) mutant Y567A]

dCTP + DNA -> Diphosphate + DNA		 (( (( (E * k_pol) ) * A) ) / (Kd + A)) buffer: 66 mM Tris-HCl, 10 mM MgSO4
PH: 7.5
Temperature: 25
- [DNA-directed DNA polymerase(Enzyme) mutant Y391A]

dCTP + DNA -> Diphosphate + DNA		 (( (( (E * k_pol) ) * A) ) / (Kd + A)) buffer: 66 mM Tris-HCl, 10 mM MgSO4
PH: 7.5
Temperature: 25
- [DNA-directed DNA polymerase(Enzyme) wildtype]

dTTP + DNA -> DNA + Diphosphate		 (( (( (E * k_pol) ) * A) ) / (Kd + A)) buffer: 66 mM Tris-HCl, 10 mM MgSO4
PH: 7.5
Temperature: 25
- [DNA-directed DNA polymerase(Enzyme) mutant Y567A]

DNA + dGTP -> DNA + Diphosphate		 (( (( (E * k_pol) ) * A) ) / (Kd + A)) buffer: 66 mM Tris-HCl, 10 mM MgSO4
PH: 7.5
Temperature: 25
- [DNA-directed DNA polymerase(Enzyme) mutant T587A]

DNA + dCTP -> DNA + Diphosphate		 (( (( (E * k_pol) ) * A) ) / (Kd + A)) buffer: 66 mM Tris-HCl, 10 mM MgSO4
PH: 7.5
Temperature: 25
- [DNA-directed DNA polymerase(Enzyme) mutant T587A/Y391F]

DNA + dCTP -> Diphosphate + DNA		 (( (( (E * k_pol) ) * A) ) / (Kd + A)) buffer: 66 mM Tris-HCl, 10 mM MgSO4
PH: 7.5
Temperature: 25
- [DNA-directed DNA polymerase(Enzyme) mutant Y567A]

DNA + dTTP -> DNA + Diphosphate		 (( (( (E * k_pol) ) * A) ) / (Kd + A)) buffer: 66 mM Tris-HCl, 10 mM MgSO4
PH: 7.5
Temperature: 25
- [DNA-directed DNA polymerase(Enzyme) mutant Y391F]

dCTP + DNA -> DNA + Diphosphate		 (( (( (E * k_pol) ) * A) ) / (Kd + A)) buffer: 66 mM Tris-HCl, 10 mM MgSO4
PH: 7.5
Temperature: 25
- [DNA-directed DNA polymerase(Enzyme) mutant Y391F]

DNA + dGTP -> Diphosphate + DNA		 (( (( (E * k_pol) ) * A) ) / (Kd + A)) buffer: 66 mM Tris-HCl, 10 mM MgSO4
PH: 7.5
Temperature: 25
- [DNA-directed DNA polymerase(Enzyme) mutant T587A/Y391A]

dCTP + DNA -> DNA + Diphosphate		 (( (( (E * k_pol) ) * A) ) / (Kd + A)) buffer: 66 mM Tris-HCl, 10 mM MgSO4
PH: 7.5
Temperature: 25
- [DNA-directed DNA polymerase(Enzyme) wildtype]

DNA + 2,4_Difluorotoluene_deoxynucleoside_triphosphate -> Diphosphate + DNA		 (( (( (E * k_pol) ) * A) ) / (Kd + A)) buffer: 66 mM Tris-HCl, 10 mM MgSO4
PH: 7.5
Temperature: 25
- [DNA-directed DNA polymerase(Enzyme) mutant Y567A]

DNA + 2,4_Difluorotoluene_deoxynucleoside_triphosphate -> Diphosphate + DNA		 (( (( (E * k_pol) ) * A) ) / (Kd + A)) buffer: 66 mM Tris-HCl, 10 mM MgSO4
PH: 7.5
Temperature: 25
uniprot:Q9UGP5 [Mn2+]

DNA + dTTP -> Diphosphate + DNA		 (Vmax / (( (( (( (1 + (Km / S)) ) + (Kd / A)) ) + (( (( (Km * Kd) ) / A) ) * S)) ) + (A / Kd_2))) buffer: 50 mM Tris-HCl, 0.25 mg/ml bovine serum albumin, 1 mM Dithiothreitol
PH: 7.6
Temperature: 37
uniprot:Q9UGP5 [Mn2+]

dTTP + DNA -> DNA + Diphosphate		 (Vmax / (( (( (( (1 + (Km / S)) ) + (Kd / A)) ) + (( (( (Km * Kd) ) / A) ) * S)) ) + (A / Kd_2))) buffer: 50 mM Tris-HCl, 0.25 mg/ml bovine serum albumin, 1 mM Dithiothreitol
PH: 7.6
Temperature: 37
uniprot:Q9UGP5 [Mg2+]

DNA + dTTP -> Diphosphate + DNA		 (Vmax / (( (( (( (1 + (Km / S)) ) + (Kd / A)) ) + (( (( (Km * Kd) ) / A) ) * S)) ) + (A / Kd_2))) buffer: 50 mM Tris-HCl, 0.25 mg/ml bovine serum albumin, 1 mM Dithiothreitol
PH: 7.6
Temperature: 37
uniprot:Q9UGP5 [Mg2+]

dTTP + DNA -> Diphosphate + DNA		 (Vmax / (( (( (( (1 + (Km / S)) ) + (Kd / A)) ) + (( (( (Km * Kd) ) / A) ) * S)) ) + (A / Kd_2))) buffer: 50 mM Tris-HCl, 0.25 mg/ml bovine serum albumin, 1 mM Dithiothreitol
PH: 7.6
Temperature: 37
uniprot:Q9UGP5 [Mg2+]

DNA + dTTP -> Diphosphate + DNA		 (Vmax / (( (( (( (1 + (Km / S)) ) + (Kd / A)) ) + (( (( (Km * Kd) ) / A) ) * S)) ) + (A / Kd_2))) buffer: 50 mM Tris-HCl, 0.25 mg/ml bovine serum albumin, 1 mM Dithiothreitol
PH: 7.6
Temperature: 37
uniprot:Q9UGP5 [Mg2+]

DNA + dTTP -> Diphosphate + DNA		 (Vmax / (( (( (( (1 + (Km / S)) ) + (Kd / A)) ) + (( (( (Km * Kd) ) / A) ) * S)) ) + (A / Kd_2))) buffer: 50 mM Tris-HCl, 0.25 mg/ml bovine serum albumin, 1 mM Dithiothreitol
PH: 7.6
Temperature: 37
uniprot:Q9UGP5 [Mn2+]

dTTP + DNA -> DNA + Diphosphate		 (Vmax / (1 + (Kd / C))) buffer: 50 mM Tris-HCl, 0.25 mg/ml bovine serum albumin, 1 mM Dithiothreitol
PH: 7.6
Temperature: 37
uniprot:P04415 [DNA-directed DNA polymerase(Enzyme) mutant D324A]

H2O + DNA -> dTMP + DNA		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 66 mM Tris, 17 mM (NH4)2SO4, 10 mM 2-mercaptoethanol, 6.5 mM MgSO4, 166 µg/ml bovine serum albumin (DNase free)
PH: 8.8
Temperature: 30
uniprot:P04415 [DNA-directed DNA polymerase(Enzyme) mutant D219A]

DNA + H2O -> DNA + dTMP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 66 mM Tris, 17 mM (NH4)2SO4, 10 mM 2-mercaptoethanol, 6.5 mM MgSO4, 166 µg/ml bovine serum albumin (DNase free)
PH: 8.8
Temperature: 30
uniprot:P04415 [DNA-directed DNA polymerase(Enzyme) mutant D219A/D324A]

H2O + DNA -> DNA + dTMP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 66 mM Tris, 17 mM (NH4)2SO4, 10 mM 2-mercaptoethanol, 6.5 mM MgSO4, 166 µg/ml bovine serum albumin (DNase free)
PH: 8.8
Temperature: 30
uniprot:P04415 [DNA-directed DNA polymerase(Enzyme) mutant D112N]

DNA + H2O -> Deoxynucleoside_5'_phosphate + DNA		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 66 mM Tris, 17 mM (NH4)2SO4, 10 mM 2-mercaptoethanol, 6.5 mM MgSO4, 166 µg/ml bovine serum albumin (DNase free)
PH: 8.8
Temperature: 30
uniprot:P04415 [DNA-directed DNA polymerase(Enzyme) wildtype]

DNA + H2O -> DNA + Deoxynucleoside_5'_phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 66 mM Tris, 17 mM (NH4)2SO4, 10 mM 2-mercaptoethanol, 6.5 mM MgSO4, 166 µg/ml bovine serum albumin (DNase free)
PH: 8.8
Temperature: 30
uniprot:P04415 [DNA-directed DNA polymerase(Enzyme) mutant D112A]

DNA + H2O -> Deoxynucleoside_5'_phosphate + DNA		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 66 mM Tris, 17 mM (NH4)2SO4, 10 mM 2-mercaptoethanol, 6.5 mM MgSO4, 166 µg/ml bovine serum albumin (DNase free)
PH: 8.8
Temperature: 30
uniprot:P04415 [DNA-directed DNA polymerase(Enzyme) mutant E114A]

DNA + H2O -> DNA + Deoxynucleoside_5'_phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 66 mM Tris, 17 mM (NH4)2SO4, 10 mM 2-mercaptoethanol, 6.5 mM MgSO4, 166 µg/ml bovine serum albumin (DNase free)
PH: 8.8
Temperature: 30
uniprot:P04415 [DNA-directed DNA polymerase(Enzyme) mutant D324A]

H2O + DNA -> DNA + Deoxynucleoside_5'_phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 66 mM Tris, 17 mM (NH4)2SO4, 10 mM 2-mercaptoethanol, 6.5 mM MgSO4, 166 µg/ml bovine serum albumin (DNase free)
PH: 8.8
Temperature: 30
uniprot:P04415 [DNA-directed DNA polymerase(Enzyme) mutant D219A]

DNA + H2O -> Deoxynucleoside_5'_phosphate + DNA		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 66 mM Tris, 17 mM (NH4)2SO4, 10 mM 2-mercaptoethanol, 6.5 mM MgSO4, 166 µg/ml bovine serum albumin (DNase free)
PH: 8.8
Temperature: 30
uniprot:P04415 [DNA-directed DNA polymerase(Enzyme) wildtype]

H2O + DNA -> dTMP + DNA		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 66 mM Tris, 17 mM (NH4)2SO4, 10 mM 2-mercaptoethanol, 6.5 mM MgSO4, 166 µg/ml bovine serum albumin (DNase free)
PH: 8.8
Temperature: 30
uniprot:P04415 [DNA-directed DNA polymerase(Enzyme) mutant E114A]

DNA + H2O -> dTMP + DNA		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 66 mM Tris, 17 mM (NH4)2SO4, 10 mM 2-mercaptoethanol, 6.5 mM MgSO4, 166 µg/ml bovine serum albumin (DNase free)
PH: 8.8
Temperature: 30
uniprot:P04415 [DNA-directed DNA polymerase(Enzyme) mutant D112A]

DNA + H2O -> dTMP + DNA		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 66 mM Tris, 17 mM (NH4)2SO4, 10 mM 2-mercaptoethanol, 6.5 mM MgSO4, 166 µg/ml bovine serum albumin (DNase free)
PH: 8.8
Temperature: 30

Pathways

pathway id name
WikiPathways:WP2472 Escherichia coli K-12 peripherome
Plant Reactome:R-OSA-9640887 G1/S transition
Plant Reactome:R-OSA-9675781 S phase
Plant Reactome:R-OSA-9675885 Lagging strand synthesis
Plant Reactome:R-OSA-9675823 DNA strand Elongation
Plant Reactome:R-OSA-9640670 Cell cycle
Plant Reactome:R-OSA-9675868 Synthesis of DNA
Plant Reactome:R-OSA-9675815 Leading strand synthesis
Plant Reactome:R-OSA-9640713 Mitosis
Plant Reactome:R-OSA-9645850 Activation of pre-replication complex
Plant Reactome:R-OSA-9675824 DNA replication Initiation
Plant Reactome:R-OSA-9675782 Maturation
Plant Reactome:R-OSA-2894886 Cellular processes
Reactome:R-HSA-9610379 HCMV Late Events
Reactome:R-HSA-9609690 HCMV Early Events
Reactome:R-HSA-9609646 HCMV Infection
Reactome:R-HSA-9645723 Diseases of programmed cell death
Reactome:R-HSA-9710421 Defective pyroptosis
Reactome:R-HSA-9824446 Viral Infection Pathways
Reactome:R-HSA-9913635 Strand-asynchronous mitochondrial DNA replication
Reactome:R-MMU-9913635 Strand-asynchronous mitochondrial DNA replication
Reactome:R-SCE-110362 POLB-Dependent Long Patch Base Excision Repair
Reactome:R-RNO-9913635 Strand-asynchronous mitochondrial DNA replication
Reactome:R-DME-9913635 Strand-asynchronous mitochondrial DNA replication
Reactome:R-CEL-5655862 Translesion synthesis by POLK
Reactome:R-CEL-5656169 Termination of translesion DNA synthesis
Reactome:R-BTA-597592 Post-translational protein modification
Reactome:R-BTA-73884 Base Excision Repair
Reactome:R-BTA-110381 Resolution of AP sites via the single-nucleotide replacement pathway
Reactome:R-BTA-110362 POLB-Dependent Long Patch Base Excision Repair
Reactome:R-CEL-69242 S Phase
Reactome:R-CEL-69091 Polymerase switching
Reactome:R-CEL-73894 DNA Repair
Reactome:R-DRE-73933 Resolution of Abasic Sites (AP sites)
Reactome:R-DRE-5649702 APEX1-Independent Resolution of AP Sites via the Single Nucleotide Replacement Pathway
Reactome:R-DRE-392499 Metabolism of proteins
Reactome:R-DRE-5689880 Ub-specific processing proteases
Reactome:R-DDI-69002 DNA Replication Pre-Initiation
Reactome:R-DDI-69239 Synthesis of DNA
Reactome:R-DDI-69190 DNA strand elongation
Reactome:R-DDI-69186 Lagging Strand Synthesis
Reactome:R-DDI-113510 E2F mediated regulation of DNA replication
Reactome:R-DME-69239 Synthesis of DNA
Reactome:R-DME-69278 Cell Cycle, Mitotic
Reactome:R-DME-110314 Recognition of DNA damage by PCNA-containing replication complex
Reactome:R-DME-5655862 Translesion synthesis by POLK
Reactome:R-HSA-110373 Resolution of AP sites via the multiple-nucleotide patch replacement pathway
Reactome:R-HSA-110314 Recognition of DNA damage by PCNA-containing replication complex
Reactome:R-HSA-5655862 Translesion synthesis by POLK
Reactome:R-HSA-5693532 DNA Double-Strand Break Repair
Reactome:R-HSA-5693538 Homology Directed Repair
Reactome:R-HSA-5358606 Mismatch repair (MMR) directed by MSH2:MSH3 (MutSbeta)
Reactome:R-HSA-69206 G1/S Transition
Reactome:R-HSA-69205 G1/S-Specific Transcription
Reactome:R-HSA-113501 Inhibition of replication initiation of damaged DNA by RB1/E2F1
Reactome:R-HSA-69239 Synthesis of DNA
Reactome:R-HSA-69091 Polymerase switching
Reactome:R-HSA-73886 Chromosome Maintenance
Reactome:R-HSA-180786 Extension of Telomeres
Reactome:R-HSA-174411 Polymerase switching on the C-strand of the telomere
Reactome:R-HSA-392499 Metabolism of proteins
Reactome:R-HSA-597592 Post-translational protein modification
Reactome:R-HSA-1643685 Disease
Reactome:R-HSA-162587 HIV Life Cycle
Reactome:R-HSA-162589 Reverse Transcription of HIV RNA
Reactome:R-HSA-175567 Integration of viral DNA into host genomic DNA
Reactome:R-HSA-162909 Host Interactions of HIV factors
Reactome:R-HSA-69002 DNA Replication Pre-Initiation
Reactome:R-MMU-1640170 Cell Cycle
Reactome:R-MMU-113510 E2F mediated regulation of DNA replication
Reactome:R-MMU-69239 Synthesis of DNA
Reactome:R-MMU-69091 Polymerase switching
Reactome:R-MMU-174417 Telomere C-strand (Lagging Strand) Synthesis
Reactome:R-MMU-174437 Removal of the Flap Intermediate from the C-strand
Reactome:R-MMU-73933 Resolution of Abasic Sites (AP sites)
Reactome:R-MMU-5649702 APEX1-Independent Resolution of AP Sites via the Single Nucleotide Replacement Pathway
Reactome:R-MMU-73893 DNA Damage Bypass
Reactome:R-MMU-110320 Translesion Synthesis by POLH
Reactome:R-MMU-5685939 HDR through MMEJ (alt-NHEJ)
Reactome:R-MMU-5358508 MMR
Reactome:R-MMU-5689880 Ub-specific processing proteases
Reactome:R-RNO-113501 Inhibition of replication initiation of damaged DNA by RB1/E2F1
Reactome:R-RNO-68952 DNA replication initiation
Reactome:R-RNO-69190 DNA strand elongation
Reactome:R-RNO-69186 Lagging Strand Synthesis
Reactome:R-RNO-157579 Telomere Maintenance
Reactome:R-RNO-174430 Telomere C-strand synthesis initiation
Reactome:R-RNO-597592 Post-translational protein modification
Reactome:R-RNO-69002 DNA Replication Pre-Initiation
Reactome:R-RNO-73894 DNA Repair
Reactome:R-RNO-110373 Resolution of AP sites via the multiple-nucleotide patch replacement pathway
Reactome:R-RNO-110314 Recognition of DNA damage by PCNA-containing replication complex
Reactome:R-RNO-5358565 Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha)
Reactome:R-SCE-110312 Translesion synthesis by REV1
Reactome:R-SCE-1640170 Cell Cycle
Reactome:R-SCE-69206 G1/S Transition
Reactome:R-SCE-113510 E2F mediated regulation of DNA replication
Reactome:R-SCE-69239 Synthesis of DNA
Reactome:R-SPO-69002 DNA Replication Pre-Initiation
Reactome:R-SPO-68962 Activation of the pre-replicative complex
Reactome:R-SPO-69091 Polymerase switching
Reactome:R-SPO-69206 G1/S Transition
Reactome:R-SPO-73893 DNA Damage Bypass
Reactome:R-BTA-5689880 Ub-specific processing proteases
Reactome:R-BTA-73894 DNA Repair
Reactome:R-BTA-110373 Resolution of AP sites via the multiple-nucleotide patch replacement pathway
Reactome:R-CEL-69278 Cell Cycle, Mitotic
Reactome:R-CEL-69109 Leading Strand Synthesis
Reactome:R-CEL-69306 DNA Replication
Reactome:R-CEL-110313 Translesion synthesis by Y family DNA polymerases bypasses lesions on DNA template
Reactome:R-DRE-73930 Abasic sugar-phosphate removal via the single-nucleotide replacement pathway
Reactome:R-DRE-5651801 PCNA-Dependent Long Patch Base Excision Repair
Reactome:R-DDI-69306 DNA Replication
Reactome:R-DDI-68962 Activation of the pre-replicative complex
Reactome:R-DDI-69091 Polymerase switching
Reactome:R-DDI-453279 Mitotic G1 phase and G1/S transition
Reactome:R-DDI-69206 G1/S Transition
Reactome:R-DME-68962 Activation of the pre-replicative complex
Reactome:R-DME-69242 S Phase
Reactome:R-DME-73894 DNA Repair
Reactome:R-DME-73893 DNA Damage Bypass
Reactome:R-DME-110320 Translesion Synthesis by POLH
Reactome:R-DME-6781827 Transcription-Coupled Nucleotide Excision Repair (TC-NER)
Reactome:R-HSA-2564830 Cytosolic iron-sulfur cluster assembly
Reactome:R-HSA-73884 Base Excision Repair
Reactome:R-HSA-73933 Resolution of Abasic Sites (AP sites)
Reactome:R-HSA-5649702 APEX1-Independent Resolution of AP Sites via the Single Nucleotide Replacement Pathway
Reactome:R-HSA-73893 DNA Damage Bypass
Reactome:R-HSA-110320 Translesion Synthesis by POLH
Reactome:R-HSA-5358565 Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha)
Reactome:R-HSA-1640170 Cell Cycle
Reactome:R-HSA-113510 E2F mediated regulation of DNA replication
Reactome:R-HSA-69109 Leading Strand Synthesis
Reactome:R-HSA-157579 Telomere Maintenance
Reactome:R-HSA-174430 Telomere C-strand synthesis initiation
Reactome:R-HSA-5689880 Ub-specific processing proteases
Reactome:R-HSA-162906 HIV Infection
Reactome:R-HSA-162585 Uncoating of the HIV Virion
Reactome:R-HSA-162592 Integration of provirus
Reactome:R-HSA-162599 Late Phase of HIV Life Cycle
Reactome:R-HSA-175474 Assembly Of The HIV Virion
Reactome:R-HSA-162588 Budding and maturation of HIV virion
Reactome:R-HSA-180910 Vpr-mediated nuclear import of PICs
Reactome:R-HSA-69306 DNA Replication
Reactome:R-MMU-453279 Mitotic G1 phase and G1/S transition
Reactome:R-MMU-69206 G1/S Transition
Reactome:R-MMU-68962 Activation of the pre-replicative complex
Reactome:R-MMU-69109 Leading Strand Synthesis
Reactome:R-MMU-174414 Processive synthesis on the C-strand of the telomere
Reactome:R-MMU-69002 DNA Replication Pre-Initiation
Reactome:R-MMU-73930 Abasic sugar-phosphate removal via the single-nucleotide replacement pathway
Reactome:R-MMU-5651801 PCNA-Dependent Long Patch Base Excision Repair
Reactome:R-MMU-110312 Translesion synthesis by REV1
Reactome:R-MMU-5656169 Termination of translesion DNA synthesis
Reactome:R-MMU-6783310 Fanconi Anemia Pathway
Reactome:R-RNO-1640170 Cell Cycle
Reactome:R-RNO-113510 E2F mediated regulation of DNA replication
Reactome:R-RNO-69239 Synthesis of DNA
Reactome:R-RNO-69091 Polymerase switching
Reactome:R-RNO-174417 Telomere C-strand (Lagging Strand) Synthesis
Reactome:R-RNO-174437 Removal of the Flap Intermediate from the C-strand
Reactome:R-RNO-392499 Metabolism of proteins
Reactome:R-RNO-5689880 Ub-specific processing proteases
Reactome:R-RNO-69306 DNA Replication
Reactome:R-RNO-73933 Resolution of Abasic Sites (AP sites)
Reactome:R-RNO-5649702 APEX1-Independent Resolution of AP Sites via the Single Nucleotide Replacement Pathway
Reactome:R-RNO-73893 DNA Damage Bypass
Reactome:R-RNO-5358508 MMR
Reactome:R-SCE-73884 Base Excision Repair
Reactome:R-SCE-110373 Resolution of AP sites via the multiple-nucleotide patch replacement pathway
Reactome:R-SCE-110313 Translesion synthesis by Y family DNA polymerases bypasses lesions on DNA template
Reactome:R-SCE-5696398 Nucleotide Excision Repair
Reactome:R-SCE-6782135 Dual incision in TC-NER
Reactome:R-SCE-453279 Mitotic G1 phase and G1/S transition
Reactome:R-SCE-68962 Activation of the pre-replicative complex
Reactome:R-SCE-69002 DNA Replication Pre-Initiation
Reactome:R-SPO-69306 DNA Replication
Reactome:R-SPO-69109 Leading Strand Synthesis
Reactome:R-SPO-1640170 Cell Cycle
Reactome:R-SPO-453279 Mitotic G1 phase and G1/S transition
Reactome:R-SPO-69242 S Phase
Reactome:R-SPO-110312 Translesion synthesis by REV1
Reactome:R-SPO-5656169 Termination of translesion DNA synthesis
Reactome:R-BTA-392499 Metabolism of proteins
Reactome:R-BTA-5688426 Deubiquitination
Reactome:R-BTA-73930 Abasic sugar-phosphate removal via the single-nucleotide replacement pathway
Reactome:R-BTA-5651801 PCNA-Dependent Long Patch Base Excision Repair
Reactome:R-CEL-1640170 Cell Cycle
Reactome:R-CEL-69239 Synthesis of DNA
Reactome:R-CEL-69186 Lagging Strand Synthesis
Reactome:R-CEL-73893 DNA Damage Bypass
Reactome:R-DRE-73894 DNA Repair
Reactome:R-DRE-110373 Resolution of AP sites via the multiple-nucleotide patch replacement pathway
Reactome:R-DDI-68952 DNA replication initiation
Reactome:R-DDI-69183 Processive synthesis on the lagging strand
Reactome:R-DDI-1640170 Cell Cycle
Reactome:R-DDI-113501 Inhibition of replication initiation of damaged DNA by RB1/E2F1
Reactome:R-DME-69306 DNA Replication
Reactome:R-DME-68952 DNA replication initiation
Reactome:R-DME-453279 Mitotic G1 phase and G1/S transition
Reactome:R-DME-69206 G1/S Transition
Reactome:R-DME-113510 E2F mediated regulation of DNA replication
Reactome:R-DME-110313 Translesion synthesis by Y family DNA polymerases bypasses lesions on DNA template
Reactome:R-DME-5656121 Translesion synthesis by POLI
Reactome:R-DME-5696398 Nucleotide Excision Repair
Reactome:R-DME-5696400 Dual Incision in GG-NER
Reactome:R-DME-6782135 Dual incision in TC-NER
Reactome:R-HSA-110381 Resolution of AP sites via the single-nucleotide replacement pathway
Reactome:R-HSA-110362 POLB-Dependent Long Patch Base Excision Repair
Reactome:R-HSA-110313 Translesion synthesis by Y family DNA polymerases bypasses lesions on DNA template
Reactome:R-HSA-5656121 Translesion synthesis by POLI
Reactome:R-HSA-6783310 Fanconi Anemia Pathway
Reactome:R-HSA-69278 Cell Cycle, Mitotic
Reactome:R-HSA-68952 DNA replication initiation
Reactome:R-HSA-69190 DNA strand elongation
Reactome:R-HSA-69186 Lagging Strand Synthesis
Reactome:R-HSA-174414 Processive synthesis on the C-strand of the telomere
Reactome:R-HSA-5688426 Deubiquitination
Reactome:R-HSA-162594 Early Phase of HIV Life Cycle
Reactome:R-HSA-164516 Minus-strand DNA synthesis
Reactome:R-HSA-164843 2-LTR circle formation
Reactome:R-HSA-176033 Interactions of Vpr with host cellular proteins
Reactome:R-MMU-113501 Inhibition of replication initiation of damaged DNA by RB1/E2F1
Reactome:R-MMU-68952 DNA replication initiation
Reactome:R-MMU-69190 DNA strand elongation
Reactome:R-MMU-69186 Lagging Strand Synthesis
Reactome:R-MMU-157579 Telomere Maintenance
Reactome:R-MMU-174430 Telomere C-strand synthesis initiation
Reactome:R-MMU-73894 DNA Repair
Reactome:R-MMU-110373 Resolution of AP sites via the multiple-nucleotide patch replacement pathway
Reactome:R-MMU-110314 Recognition of DNA damage by PCNA-containing replication complex
Reactome:R-MMU-5655862 Translesion synthesis by POLK
Reactome:R-MMU-5358565 Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha)
Reactome:R-MMU-392499 Metabolism of proteins
Reactome:R-MMU-597592 Post-translational protein modification
Reactome:R-RNO-69278 Cell Cycle, Mitotic
Reactome:R-RNO-69242 S Phase
Reactome:R-RNO-69183 Processive synthesis on the lagging strand
Reactome:R-RNO-73886 Chromosome Maintenance
Reactome:R-RNO-180786 Extension of Telomeres
Reactome:R-RNO-174411 Polymerase switching on the C-strand of the telomere
Reactome:R-RNO-5688426 Deubiquitination
Reactome:R-RNO-73884 Base Excision Repair
Reactome:R-RNO-110381 Resolution of AP sites via the single-nucleotide replacement pathway
Reactome:R-RNO-110362 POLB-Dependent Long Patch Base Excision Repair
Reactome:R-RNO-110313 Translesion synthesis by Y family DNA polymerases bypasses lesions on DNA template
Reactome:R-RNO-5358606 Mismatch repair (MMR) directed by MSH2:MSH3 (MutSbeta)
Reactome:R-SCE-73933 Resolution of Abasic Sites (AP sites)
Reactome:R-SCE-73893 DNA Damage Bypass
Reactome:R-SCE-6781827 Transcription-Coupled Nucleotide Excision Repair (TC-NER)
Reactome:R-SCE-113501 Inhibition of replication initiation of damaged DNA by RB1/E2F1
Reactome:R-SCE-68952 DNA replication initiation
Reactome:R-SPO-69239 Synthesis of DNA
Reactome:R-SPO-69186 Lagging Strand Synthesis
Reactome:R-SPO-113510 E2F mediated regulation of DNA replication
Reactome:R-SPO-5655862 Translesion synthesis by POLK
Reactome:R-BTA-73933 Resolution of Abasic Sites (AP sites)
Reactome:R-BTA-5649702 APEX1-Independent Resolution of AP Sites via the Single Nucleotide Replacement Pathway
Reactome:R-CEL-69190 DNA strand elongation
Reactome:R-CEL-69183 Processive synthesis on the lagging strand
Reactome:R-CEL-110314 Recognition of DNA damage by PCNA-containing replication complex
Reactome:R-DRE-73884 Base Excision Repair
Reactome:R-DRE-110381 Resolution of AP sites via the single-nucleotide replacement pathway
Reactome:R-DRE-110362 POLB-Dependent Long Patch Base Excision Repair
Reactome:R-DRE-597592 Post-translational protein modification
Reactome:R-DRE-5688426 Deubiquitination
Reactome:R-DDI-69109 Leading Strand Synthesis
Reactome:R-DDI-69278 Cell Cycle, Mitotic
Reactome:R-DDI-69242 S Phase
Reactome:R-DME-69002 DNA Replication Pre-Initiation
Reactome:R-DME-1640170 Cell Cycle
Reactome:R-DME-113501 Inhibition of replication initiation of damaged DNA by RB1/E2F1
Reactome:R-DME-110312 Translesion synthesis by REV1
Reactome:R-DME-5656169 Termination of translesion DNA synthesis
Reactome:R-DME-5696399 Global Genome Nucleotide Excision Repair (GG-NER)
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-73894 DNA Repair
Reactome:R-HSA-73930 Abasic sugar-phosphate removal via the single-nucleotide replacement pathway
Reactome:R-HSA-5651801 PCNA-Dependent Long Patch Base Excision Repair
Reactome:R-HSA-110312 Translesion synthesis by REV1
Reactome:R-HSA-5656169 Termination of translesion DNA synthesis
Reactome:R-HSA-5685939 HDR through MMEJ (alt-NHEJ)
Reactome:R-HSA-5358508 MMR
Reactome:R-HSA-453279 Mitotic G1 phase and G1/S transition
Reactome:R-HSA-68962 Activation of the pre-replicative complex
Reactome:R-HSA-69242 S Phase
Reactome:R-HSA-69183 Processive synthesis on the lagging strand
Reactome:R-HSA-174417 Telomere C-strand (Lagging Strand) Synthesis
Reactome:R-HSA-174437 Removal of the Flap Intermediate from the C-strand
Reactome:R-HSA-5663205 Infectious disease
Reactome:R-HSA-173107 Binding and entry of HIV virion
Reactome:R-HSA-164525 Plus-strand DNA synthesis
Reactome:R-HSA-177539 Autointegration results in viral DNA circles
Reactome:R-HSA-180689 APOBEC3G mediated resistance to HIV-1 infection
Reactome:R-MMU-69278 Cell Cycle, Mitotic
Reactome:R-MMU-69242 S Phase
Reactome:R-MMU-69183 Processive synthesis on the lagging strand
Reactome:R-MMU-73886 Chromosome Maintenance
Reactome:R-MMU-180786 Extension of Telomeres
Reactome:R-MMU-174411 Polymerase switching on the C-strand of the telomere
Reactome:R-MMU-69306 DNA Replication
Reactome:R-MMU-73884 Base Excision Repair
Reactome:R-MMU-110381 Resolution of AP sites via the single-nucleotide replacement pathway
Reactome:R-MMU-110362 POLB-Dependent Long Patch Base Excision Repair
Reactome:R-MMU-110313 Translesion synthesis by Y family DNA polymerases bypasses lesions on DNA template
Reactome:R-MMU-5656121 Translesion synthesis by POLI
Reactome:R-MMU-5693532 DNA Double-Strand Break Repair
Reactome:R-MMU-5693538 Homology Directed Repair
Reactome:R-MMU-5358606 Mismatch repair (MMR) directed by MSH2:MSH3 (MutSbeta)
Reactome:R-MMU-5688426 Deubiquitination
Reactome:R-RNO-453279 Mitotic G1 phase and G1/S transition
Reactome:R-RNO-69206 G1/S Transition
Reactome:R-RNO-68962 Activation of the pre-replicative complex
Reactome:R-RNO-69109 Leading Strand Synthesis
Reactome:R-RNO-174414 Processive synthesis on the C-strand of the telomere
Reactome:R-RNO-73930 Abasic sugar-phosphate removal via the single-nucleotide replacement pathway
Reactome:R-RNO-5651801 PCNA-Dependent Long Patch Base Excision Repair
Reactome:R-RNO-5656169 Termination of translesion DNA synthesis
Reactome:R-SCE-73894 DNA Repair
Reactome:R-SCE-69278 Cell Cycle, Mitotic
Reactome:R-SCE-69242 S Phase
Reactome:R-SCE-69306 DNA Replication
Reactome:R-SPO-68952 DNA replication initiation
Reactome:R-SPO-69190 DNA strand elongation
Reactome:R-SPO-69183 Processive synthesis on the lagging strand
Reactome:R-SPO-69278 Cell Cycle, Mitotic
Reactome:R-SPO-113501 Inhibition of replication initiation of damaged DNA by RB1/E2F1
Reactome:R-SPO-73894 DNA Repair
Reactome:R-SPO-110313 Translesion synthesis by Y family DNA polymerases bypasses lesions on DNA template
Reactome:R-SPO-5656121 Translesion synthesis by POLI
WikiPathways:WP4016 DNA IR-damage and cellular response via ATR