EC: 2.7.7.49

RNA-directed DNA polymerase (deoxynucleoside-triphosphate:DNA deoxynucleotidyltransferase (RNA-directed))

enzyme lambda metabolic reaction experiment
uniprot:P03366 [RNA-directed DNA polymerase(Enzyme) wildtype]

dCTP + DNA -> DNA + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 0.025 % Triton X-100
PH: 7.8
Temperature: 37
uniprot:P03366 [RNA-directed DNA polymerase(Enzyme) mutant K103N]

dCTP + DNA -> Diphosphate + DNA		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 0.025 % Triton X-100
PH: 7.8
Temperature: 37
uniprot:P03366 [RNA-directed DNA polymerase(Enzyme) mutant L74V]

dCTP + DNA -> DNA + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 0.025 % Triton X-100
PH: 7.8
Temperature: 37
uniprot:P03366 [RNA-directed DNA polymerase(Enzyme) mutant Q151M]

DNA + dCTP -> DNA + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 0.025 % Triton X-100
PH: 7.8
Temperature: 37
uniprot:P03366 [RNA-directed DNA polymerase(Enzyme) mutant K65R]

dCTP + DNA -> DNA + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 0.025 % Triton X-100
PH: 7.8
Temperature: 37
uniprot:P03366 [RNA-directed DNA polymerase(Enzyme) wildtype]

dGTP + DNA -> DNA + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 0.025 % Triton X-100
PH: 7.8
Temperature: 37
uniprot:P03366 [RNA-directed DNA polymerase(Enzyme) mutant K65R/K103N/Q151M]

DNA + dCTP -> Diphosphate + DNA		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 0.025 % Triton X-100
PH: 7.8
Temperature: 37
uniprot:P03366 [RNA-directed DNA polymerase(Enzyme) mutant K65R]

dGTP + DNA -> Diphosphate + DNA		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 0.025 % Triton X-100
PH: 7.8
Temperature: 37
uniprot:P03366 [RNA-directed DNA polymerase(Enzyme) mutant D67N/K70R/T215Y/K219Q]

dCTP + DNA -> Diphosphate + DNA		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 0.025 % Triton X-100
PH: 7.8
Temperature: 37
uniprot:P03366 [RNA-directed DNA polymerase(Enzyme) mutant L74V/K103N]

DNA + dCTP -> Diphosphate + DNA		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 0.025 % Triton X-100
PH: 7.8
Temperature: 37
uniprot:P03366 [RNA-directed DNA polymerase(Enzyme) mutant K65R/Q151M]

DNA + dCTP -> DNA + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 0.025 % Triton X-100
PH: 7.8
Temperature: 37
uniprot:P03366 [RNA-directed DNA polymerase(Enzyme) mutant K65R/K103N]

DNA + dCTP -> Diphosphate + DNA		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 0.025 % Triton X-100
PH: 7.8
Temperature: 37
uniprot:P03366 [RNA-directed DNA polymerase(Enzyme) mutant M184V]

dCTP + DNA -> DNA + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 0.025 % Triton X-100
PH: 7.8
Temperature: 37
uniprot:P03366 [RNA-directed DNA polymerase(Enzyme) mutant K103N/Q151M]

dCTP + DNA -> DNA + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 0.025 % Triton X-100
PH: 7.8
Temperature: 37
uniprot:P03366 [RNA-directed DNA polymerase(Enzyme) mutant L74V]

DNA + dGTP -> Diphosphate + DNA		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 0.025 % Triton X-100
PH: 7.8
Temperature: 37
uniprot:P03366 [RNA-directed DNA polymerase(Enzyme) mutant K103N/Q151M]

dGTP + DNA -> DNA + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 0.025 % Triton X-100
PH: 7.8
Temperature: 37
uniprot:P03366 [RNA-directed DNA polymerase(Enzyme) mutant K65R/K103N/Q151M]

DNA + dGTP -> DNA + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 0.025 % Triton X-100
PH: 7.8
Temperature: 37
uniprot:P03366 [RNA-directed DNA polymerase(Enzyme) mutant D67N/K70R/T215Y/K219Q]

DNA + dGTP -> DNA + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 0.025 % Triton X-100
PH: 7.8
Temperature: 37
uniprot:P03366 [RNA-directed DNA polymerase(Enzyme) mutant Q151M]

dGTP + DNA -> Diphosphate + DNA		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 0.025 % Triton X-100
PH: 7.8
Temperature: 37
uniprot:P03366 [RNA-directed DNA polymerase(Enzyme) mutant K65R/Q151M]

dGTP + DNA -> DNA + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 0.025 % Triton X-100
PH: 7.8
Temperature: 37
uniprot:P03366 [RNA-directed DNA polymerase(Enzyme) mutant K103N]

dGTP + DNA -> Diphosphate + DNA		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 0.025 % Triton X-100
PH: 7.8
Temperature: 37
uniprot:P03366 [RNA-directed DNA polymerase(Enzyme) mutant K65R/K103N]

DNA + dGTP -> Diphosphate + DNA		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 0.025 % Triton X-100
PH: 7.8
Temperature: 37
uniprot:P03366 [RNA-directed DNA polymerase(Enzyme) mutant L74V/K103N]

dGTP + DNA -> Diphosphate + DNA		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 0.025 % Triton X-100
PH: 7.8
Temperature: 37
uniprot:P03366 [RNA-directed DNA polymerase(Enzyme) wildtype]

dTTP + DNA -> DNA + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 0.025 % Triton X-100
PH: 7.8
Temperature: 37
uniprot:P03366 [RNA-directed DNA polymerase(Enzyme) mutant K65R]

DNA + dTTP -> DNA + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 0.025 % Triton X-100
PH: 7.8
Temperature: 37
uniprot:P03366 [RNA-directed DNA polymerase(Enzyme) mutant L74V]

DNA + dTTP -> DNA + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 0.025 % Triton X-100
PH: 7.8
Temperature: 37
uniprot:P03366 [RNA-directed DNA polymerase(Enzyme) mutant K103N/Q151M]

DNA + dTTP -> DNA + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 0.025 % Triton X-100
PH: 7.8
Temperature: 37
uniprot:P03366 [RNA-directed DNA polymerase(Enzyme) mutant K103N]

DNA + dTTP -> DNA + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 0.025 % Triton X-100
PH: 7.8
Temperature: 37
uniprot:P03366 [RNA-directed DNA polymerase(Enzyme) mutant Q151M]

DNA + dTTP -> Diphosphate + DNA		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 0.025 % Triton X-100
PH: 7.8
Temperature: 37
uniprot:P03366 [RNA-directed DNA polymerase(Enzyme) mutant K65R/K103N]

DNA + dTTP -> DNA + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 0.025 % Triton X-100
PH: 7.8
Temperature: 37
uniprot:P03366 [RNA-directed DNA polymerase(Enzyme) mutant M184V]

DNA + dGTP -> Diphosphate + DNA		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 0.025 % Triton X-100
PH: 7.8
Temperature: 37
uniprot:P03366 [RNA-directed DNA polymerase(Enzyme) mutant L74V/K103N]

DNA + dTTP -> Diphosphate + DNA		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 0.025 % Triton X-100
PH: 7.8
Temperature: 37
uniprot:P03366 [RNA-directed DNA polymerase(Enzyme) mutant K65R/K103N/Q151M]

dTTP + DNA -> DNA + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 0.025 % Triton X-100
PH: 7.8
Temperature: 37
uniprot:P03366 [RNA-directed DNA polymerase(Enzyme) mutant K65R/Q151M]

DNA + dTTP -> DNA + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 0.025 % Triton X-100
PH: 7.8
Temperature: 37
uniprot:P03366 [RNA-directed DNA polymerase(Enzyme) mutant D67N/K70R/T215Y/K219Q]

DNA + dTTP -> DNA + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 0.025 % Triton X-100
PH: 7.8
Temperature: 37
uniprot:P03366 [RNA-directed DNA polymerase(Enzyme) mutant M184V]

dTTP + DNA -> Diphosphate + DNA		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 0.025 % Triton X-100
PH: 7.8
Temperature: 37
- [RNA-directed DNA polymerase(Enzyme) mutant A114S]

dTTP + DNA -> DNA + Diphosphate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Hepes, 15 mM magnesium acetate, 15 mM NaCl, 130 mM potassium acetate, 1 mM DTT, 5% poly(ethyleneglycol)
PH: 7
Temperature: 37
- [RNA-directed DNA polymerase(Enzyme) mutant A114G]

DNA + dTTP -> Diphosphate + DNA		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Hepes, 15 mM magnesium acetate, 15 mM NaCl, 130 mM potassium acetate, 1 mM DTT, 5% poly(ethyleneglycol)
PH: 7
Temperature: 37
- [RNA-directed DNA polymerase(Enzyme) mutant A114V]

dTTP + DNA -> DNA + Diphosphate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Hepes, 15 mM magnesium acetate, 15 mM NaCl, 130 mM potassium acetate, 1 mM DTT, 5% poly(ethyleneglycol)
PH: 7
Temperature: 37
- [RNA-directed DNA polymerase(Enzyme) wildtype]

DNA + dTTP -> Diphosphate + DNA		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Hepes, 15 mM magnesium acetate, 15 mM NaCl, 130 mM potassium acetate, 1 mM DTT, 5% poly(ethyleneglycol)
PH: 7
Temperature: 37
- [RNA-directed DNA polymerase(Enzyme) mutant A114T]

DNA + dTTP -> DNA + Diphosphate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Hepes, 15 mM magnesium acetate, 15 mM NaCl, 130 mM potassium acetate, 1 mM DTT, 5% poly(ethyleneglycol)
PH: 7
Temperature: 37
- [RNA-directed DNA polymerase(Enzyme) mutant A114S]

3'_Azido_3'_deoxythymidine_triphosphate + DNA -> DNA + Diphosphate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Hepes, 15 mM magnesium acetate, 15 mM NaCl, 130 mM potassium acetate, 1 mM DTT, 5% poly(ethyleneglycol)
PH: 7
Temperature: 37
- [RNA-directed DNA polymerase(Enzyme) mutant A114G]

3'_Azido_3'_deoxythymidine_triphosphate + DNA -> DNA + Diphosphate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Hepes, 15 mM magnesium acetate, 15 mM NaCl, 130 mM potassium acetate, 1 mM DTT, 5% poly(ethyleneglycol)
PH: 7
Temperature: 37
- [RNA-directed DNA polymerase(Enzyme) wildtype]

3'_Azido_3'_deoxythymidine_triphosphate + DNA -> DNA + Diphosphate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Hepes, 15 mM magnesium acetate, 15 mM NaCl, 130 mM potassium acetate, 1 mM DTT, 5% poly(ethyleneglycol)
PH: 7
Temperature: 37
- [RNA-directed DNA polymerase(Enzyme) mutant A114G]

DNA + ddTTP -> Diphosphate + DNA		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Hepes, 15 mM magnesium acetate, 15 mM NaCl, 130 mM potassium acetate, 1 mM DTT, 5% poly(ethyleneglycol)
PH: 7
Temperature: 37
- [RNA-directed DNA polymerase(Enzyme) mutant A114V]

DNA + ddTTP -> Diphosphate + DNA		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Hepes, 15 mM magnesium acetate, 15 mM NaCl, 130 mM potassium acetate, 1 mM DTT, 5% poly(ethyleneglycol)
PH: 7
Temperature: 37
- [RNA-directed DNA polymerase(Enzyme) mutant A114S]

ddTTP + DNA -> Diphosphate + DNA		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Hepes, 15 mM magnesium acetate, 15 mM NaCl, 130 mM potassium acetate, 1 mM DTT, 5% poly(ethyleneglycol)
PH: 7
Temperature: 37
- [RNA-directed DNA polymerase(Enzyme) wildtype]

DNA + ddTTP -> DNA + Diphosphate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Hepes, 15 mM magnesium acetate, 15 mM NaCl, 130 mM potassium acetate, 1 mM DTT, 5% poly(ethyleneglycol)
PH: 7
Temperature: 37
- [RNA-directed DNA polymerase(Enzyme) wildtype]

dATP + DNA -> Diphosphate + DNA		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Hepes, 15 mM magnesium acetate, 15 mM NaCl, 130 mM potassium acetate, 1 mM DTT, 5% poly(ethyleneglycol)
PH: 7
Temperature: 37
- [RNA-directed DNA polymerase(Enzyme) mutant A114S]

DNA + dATP -> Diphosphate + DNA		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Hepes, 15 mM magnesium acetate, 15 mM NaCl, 130 mM potassium acetate, 1 mM DTT, 5% poly(ethyleneglycol)
PH: 7
Temperature: 37
- [RNA-directed DNA polymerase(Enzyme) wildtype]

DNA + dCTP -> Diphosphate + DNA		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Hepes, 15 mM magnesium acetate, 15 mM NaCl, 130 mM potassium acetate, 1 mM DTT, 5% poly(ethyleneglycol)
PH: 7
Temperature: 37
- [RNA-directed DNA polymerase(Enzyme) mutant A114G]

dATP + DNA -> Diphosphate + DNA		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Hepes, 15 mM magnesium acetate, 15 mM NaCl, 130 mM potassium acetate, 1 mM DTT, 5% poly(ethyleneglycol)
PH: 7
Temperature: 37
- [RNA-directed DNA polymerase(Enzyme) mutant A114G]

dCTP + DNA -> Diphosphate + DNA		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Hepes, 15 mM magnesium acetate, 15 mM NaCl, 130 mM potassium acetate, 1 mM DTT, 5% poly(ethyleneglycol)
PH: 7
Temperature: 37
- [RNA-directed DNA polymerase(Enzyme) mutant A114S]

dCTP + DNA -> Diphosphate + DNA		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Hepes, 15 mM magnesium acetate, 15 mM NaCl, 130 mM potassium acetate, 1 mM DTT, 5% poly(ethyleneglycol)
PH: 7
Temperature: 37
- [RNA-directed DNA polymerase(Enzyme) wildtype]

DNA + ddCTP -> Diphosphate + DNA		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Hepes, 15 mM magnesium acetate, 15 mM NaCl, 130 mM potassium acetate, 1 mM DTT, 5% poly(ethyleneglycol)
PH: 7
Temperature: 37
- [RNA-directed DNA polymerase(Enzyme) mutant A114G]

ddCTP + DNA -> DNA + Diphosphate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Hepes, 15 mM magnesium acetate, 15 mM NaCl, 130 mM potassium acetate, 1 mM DTT, 5% poly(ethyleneglycol)
PH: 7
Temperature: 37
- [RNA-directed DNA polymerase(Enzyme) mutant A114S]

DNA + ddCTP -> DNA + Diphosphate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Hepes, 15 mM magnesium acetate, 15 mM NaCl, 130 mM potassium acetate, 1 mM DTT, 5% poly(ethyleneglycol)
PH: 7
Temperature: 37
- [RNA-directed DNA polymerase(Enzyme) wildtype]

dCTP + DNA -> Diphosphate + DNA		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Hepes, 15 mM magnesium acetate, 15 mM NaCl, 130 mM potassium acetate, 1 mM DTT, 5% poly(ethyleneglycol)
PH: 7
Temperature: 37
- [RNA-directed DNA polymerase(Enzyme) mutant A114G]

dCTP + DNA -> DNA + Diphosphate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Hepes, 15 mM magnesium acetate, 15 mM NaCl, 130 mM potassium acetate, 1 mM DTT, 5% poly(ethyleneglycol)
PH: 7
Temperature: 37
- [RNA-directed DNA polymerase(Enzyme) mutant A114S]

dCTP + DNA -> Diphosphate + DNA		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Hepes, 15 mM magnesium acetate, 15 mM NaCl, 130 mM potassium acetate, 1 mM DTT, 5% poly(ethyleneglycol)
PH: 7
Temperature: 37
- [RNA-directed DNA polymerase(Enzyme) wildtype]

ATP + DNA -> Diphosphate + DNA		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Hepes, 15 mM magnesium acetate, 15 mM NaCl, 130 mM potassium acetate, 1 mM DTT, 5% poly(ethyleneglycol)
PH: 7
Temperature: 37
- [RNA-directed DNA polymerase(Enzyme) mutant A114S]

ATP + DNA -> Diphosphate + DNA		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Hepes, 15 mM magnesium acetate, 15 mM NaCl, 130 mM potassium acetate, 1 mM DTT, 5% poly(ethyleneglycol)
PH: 7
Temperature: 37
- [RNA-directed DNA polymerase(Enzyme) mutant A114G]

DNA + ATP -> Diphosphate + DNA		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Hepes, 15 mM magnesium acetate, 15 mM NaCl, 130 mM potassium acetate, 1 mM DTT, 5% poly(ethyleneglycol)
PH: 7
Temperature: 37
- [RNA-directed DNA polymerase(Enzyme) mutant A114G]

DNA + ddATP -> DNA + Diphosphate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Hepes, 15 mM magnesium acetate, 15 mM NaCl, 130 mM potassium acetate, 1 mM DTT, 5% poly(ethyleneglycol)
PH: 7
Temperature: 37
- [RNA-directed DNA polymerase(Enzyme) wildtype]

DNA + ddATP -> Diphosphate + DNA		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Hepes, 15 mM magnesium acetate, 15 mM NaCl, 130 mM potassium acetate, 1 mM DTT, 5% poly(ethyleneglycol)
PH: 7
Temperature: 37
- [RNA-directed DNA polymerase(Enzyme) mutant A114S]

ddATP + DNA -> DNA + Diphosphate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Hepes, 15 mM magnesium acetate, 15 mM NaCl, 130 mM potassium acetate, 1 mM DTT, 5% poly(ethyleneglycol)
PH: 7
Temperature: 37
- [RNA-directed DNA polymerase(Enzyme) wildtype]

DNA + dGTP -> DNA + Diphosphate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Hepes, 15 mM magnesium acetate, 15 mM NaCl, 130 mM potassium acetate, 1 mM DTT, 5% poly(ethyleneglycol)
PH: 7
Temperature: 37
- [RNA-directed DNA polymerase(Enzyme) wildtype]

dTTP + DNA -> Diphosphate + DNA		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Hepes, 15 mM magnesium acetate, 15 mM NaCl, 130 mM potassium acetate, 1 mM DTT, 5% poly(ethyleneglycol)
PH: 7
Temperature: 37
- [RNA-directed DNA polymerase(Enzyme) mutant A114S]

dTTP + DNA -> DNA + Diphosphate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Hepes, 15 mM magnesium acetate, 15 mM NaCl, 130 mM potassium acetate, 1 mM DTT, 5% poly(ethyleneglycol)
PH: 7
Temperature: 37
- [RNA-directed DNA polymerase(Enzyme) mutant A114G]

dGTP + DNA -> DNA + Diphosphate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Hepes, 15 mM magnesium acetate, 15 mM NaCl, 130 mM potassium acetate, 1 mM DTT, 5% poly(ethyleneglycol)
PH: 7
Temperature: 37
- [RNA-directed DNA polymerase(Enzyme) mutant A114G]

DNA + dTTP -> Diphosphate + DNA		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Hepes, 15 mM magnesium acetate, 15 mM NaCl, 130 mM potassium acetate, 1 mM DTT, 5% poly(ethyleneglycol)
PH: 7
Temperature: 37
- [RNA-directed DNA polymerase(Enzyme) mutant A114S]

dGTP + DNA -> DNA + Diphosphate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Hepes, 15 mM magnesium acetate, 15 mM NaCl, 130 mM potassium acetate, 1 mM DTT, 5% poly(ethyleneglycol)
PH: 7
Temperature: 37
- [RNA-directed DNA polymerase(Enzyme) wildtype]

DNA + dTTP -> Diphosphate + DNA		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Hepes, 15 mM magnesium acetate, 15 mM NaCl, 130 mM potassium acetate, 1 mM DTT, 5% poly(ethyleneglycol)
PH: 7
Temperature: 37
- [RNA-directed DNA polymerase(Enzyme) mutant A114G]

DNA + dTTP -> Diphosphate + DNA		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Hepes, 15 mM magnesium acetate, 15 mM NaCl, 130 mM potassium acetate, 1 mM DTT, 5% poly(ethyleneglycol)
PH: 7
Temperature: 37
- [RNA-directed DNA polymerase(Enzyme) wildtype]

dTTP + DNA -> DNA + Diphosphate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Hepes, 15 mM magnesium acetate, 15 mM NaCl, 130 mM potassium acetate, 1 mM DTT, 5% poly(ethyleneglycol)
PH: 7
Temperature: 37
- [RNA-directed DNA polymerase(Enzyme) mutant A114S]

dTTP + DNA -> DNA + Diphosphate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Hepes, 15 mM magnesium acetate, 15 mM NaCl, 130 mM potassium acetate, 1 mM DTT, 5% poly(ethyleneglycol)
PH: 7
Temperature: 37
- [RNA-directed DNA polymerase(Enzyme) mutant A114S]

DNA + dTTP -> DNA + Diphosphate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Hepes, 15 mM magnesium acetate, 15 mM NaCl, 130 mM potassium acetate, 1 mM DTT, 5% poly(ethyleneglycol)
PH: 7
Temperature: 37
- [RNA-directed DNA polymerase(Enzyme) mutant A114G]

DNA + dTTP -> DNA + Diphosphate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Hepes, 15 mM magnesium acetate, 15 mM NaCl, 130 mM potassium acetate, 1 mM DTT, 5% poly(ethyleneglycol)
PH: 7
Temperature: 37

Pathways

pathway id name
Reactome:R-HSA-9856651 MITF-M-dependent gene expression
Reactome:R-HSA-9824446 Viral Infection Pathways
Reactome:R-HSA-9730414 MITF-M-regulated melanocyte development
Reactome:R-HSA-9825895 Regulation of MITF-M-dependent genes involved in DNA replication, damage repair and senescence
Reactome:R-HSA-5693532 DNA Double-Strand Break Repair
Reactome:R-HSA-5693538 Homology Directed Repair
Reactome:R-HSA-201681 TCF dependent signaling in response to WNT
Reactome:R-HSA-73886 Chromosome Maintenance
Reactome:R-HSA-180786 Extension of Telomeres
Reactome:R-HSA-1643685 Disease
Reactome:R-HSA-162587 HIV Life Cycle
Reactome:R-HSA-162589 Reverse Transcription of HIV RNA
Reactome:R-HSA-175567 Integration of viral DNA into host genomic DNA
Reactome:R-HSA-162909 Host Interactions of HIV factors
Reactome:R-MMU-1640170 Cell Cycle
Reactome:R-MMU-5685939 HDR through MMEJ (alt-NHEJ)
Reactome:R-MMU-162582 Signaling Pathways
Reactome:R-RNO-157579 Telomere Maintenance
Reactome:R-RNO-162582 Signaling Pathways
Reactome:R-HSA-1266738 Developmental Biology
Reactome:R-HSA-195721 Signaling by WNT
Reactome:R-HSA-201722 Formation of the beta-catenin:TCF transactivating complex
Reactome:R-HSA-1640170 Cell Cycle
Reactome:R-HSA-157579 Telomere Maintenance
Reactome:R-HSA-162906 HIV Infection
Reactome:R-HSA-162585 Uncoating of the HIV Virion
Reactome:R-HSA-162592 Integration of provirus
Reactome:R-HSA-162599 Late Phase of HIV Life Cycle
Reactome:R-HSA-175474 Assembly Of The HIV Virion
Reactome:R-HSA-162588 Budding and maturation of HIV virion
Reactome:R-HSA-180910 Vpr-mediated nuclear import of PICs
Reactome:R-MMU-171319 Telomere Extension By Telomerase
Reactome:R-RNO-1640170 Cell Cycle
Reactome:R-HSA-171319 Telomere Extension By Telomerase
Reactome:R-HSA-162594 Early Phase of HIV Life Cycle
Reactome:R-HSA-164516 Minus-strand DNA synthesis
Reactome:R-HSA-164843 2-LTR circle formation
Reactome:R-HSA-176033 Interactions of Vpr with host cellular proteins
Reactome:R-MMU-157579 Telomere Maintenance
Reactome:R-MMU-73894 DNA Repair
Reactome:R-MMU-195721 Signaling by WNT
Reactome:R-MMU-201722 Formation of the beta-catenin:TCF transactivating complex
Reactome:R-RNO-73886 Chromosome Maintenance
Reactome:R-RNO-180786 Extension of Telomeres
Reactome:R-RNO-195721 Signaling by WNT
Reactome:R-RNO-201722 Formation of the beta-catenin:TCF transactivating complex
Reactome:R-HSA-73894 DNA Repair
Reactome:R-HSA-5685939 HDR through MMEJ (alt-NHEJ)
Reactome:R-HSA-162582 Signaling Pathways
Reactome:R-HSA-5663205 Infectious disease
Reactome:R-HSA-173107 Binding and entry of HIV virion
Reactome:R-HSA-164525 Plus-strand DNA synthesis
Reactome:R-HSA-177539 Autointegration results in viral DNA circles
Reactome:R-HSA-180689 APOBEC3G mediated resistance to HIV-1 infection
Reactome:R-MMU-73886 Chromosome Maintenance
Reactome:R-MMU-180786 Extension of Telomeres
Reactome:R-MMU-5693532 DNA Double-Strand Break Repair
Reactome:R-MMU-5693538 Homology Directed Repair
Reactome:R-MMU-201681 TCF dependent signaling in response to WNT
Reactome:R-RNO-171319 Telomere Extension By Telomerase
Reactome:R-RNO-201681 TCF dependent signaling in response to WNT
WikiPathways:WP5121 Sphingolipid metabolism in senescence