EC: 2.7.7.31

DNA nucleotidylexotransferase (nucleoside-triphosphate:DNA deoxynucleotidylexotransferase)

enzyme lambda metabolic reaction experiment
uniprot:P04053 [DNA nucleotidylexotransferase(Enzyme) mutant R336Q]

n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_DNA + dATP -> Diphosphate + n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_DNA		 (( (( (E * kcat) ) * A) ) / (Ka + A)) buffer: 200 mM potassium cacodylate, 8 mM MgCl2, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 35
uniprot:P04053 [DNA nucleotidylexotransferase(Enzyme) mutant R336A]

dATP + n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_DNA -> Diphosphate + n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_DNA		 (( (( (E * kcat) ) * A) ) / (Ka + A)) buffer: 200 mM potassium cacodylate, 8 mM MgCl2, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 35
uniprot:P04053 [DNA nucleotidylexotransferase(Enzyme) wildtype]

n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_DNA + dATP -> n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_DNA + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Ka + A)) buffer: 200 mM potassium cacodylate, 8 mM MgCl2, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 35
uniprot:P04053 [DNA nucleotidylexotransferase(Enzyme) mutant D343E]

dATP + n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_DNA -> n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_DNA + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Ka + A)) buffer: 200 mM potassium cacodylate, 8 mM MgCl2, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 35
uniprot:P04053 [DNA nucleotidylexotransferase(Enzyme) mutant D345E]

n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_DNA + dATP -> Diphosphate + n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_DNA		 (( (( (E * kcat) ) * A) ) / (Ka + A)) buffer: 200 mM potassium cacodylate, 8 mM MgCl2, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 35
uniprot:P04053 [DNA nucleotidylexotransferase(Enzyme) wildtype]

n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_DNA + dATP -> n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_DNA + Diphosphate		 (( (( (E * kcat) ) * B) ) / (Kb + B)) buffer: 200 mM potassium cacodylate, 8 mM MgCl2, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 35
uniprot:P04053 [DNA nucleotidylexotransferase(Enzyme) mutant R336A]

dATP + n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_DNA -> n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_DNA + Diphosphate		 (( (( (E * kcat) ) * B) ) / (Kb + B)) buffer: 200 mM potassium cacodylate, 8 mM MgCl2, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 35
uniprot:P04053 [DNA nucleotidylexotransferase(Enzyme) mutant D345E]

n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_DNA + dATP -> n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_DNA + Diphosphate		 (( (( (E * kcat) ) * B) ) / (Kb + B)) buffer: 200 mM potassium cacodylate, 8 mM MgCl2, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 35
uniprot:P04053 [DNA nucleotidylexotransferase(Enzyme) mutant D343E]

n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_DNA + dATP -> n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_DNA + Diphosphate		 (( (( (E * kcat) ) * B) ) / (Kb + B)) buffer: 200 mM potassium cacodylate, 8 mM MgCl2, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 35
uniprot:P04053 [DNA nucleotidylexotransferase(Enzyme) mutant R336Q]

dATP + n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_DNA -> Diphosphate + n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_DNA		 (( (( (E * kcat) ) * B) ) / (Kb + B)) buffer: 200 mM potassium cacodylate, 8 mM MgCl2, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 35
uniprot:P04053 [DNA nucleotidylexotransferase(Enzyme) wildtype]

n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_DNA + dGTP -> n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_DNA + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Ka + A)) buffer: 200 mM potassium cacodylate, 8 mM MgCl2, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 35
uniprot:P04053 [DNA nucleotidylexotransferase(Enzyme) mutant R336Q]

n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_DNA + dGTP -> Diphosphate + n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_DNA		 (( (( (E * kcat) ) * A) ) / (Ka + A)) buffer: 200 mM potassium cacodylate, 8 mM MgCl2, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 35
uniprot:P04053 [DNA nucleotidylexotransferase(Enzyme) wildtype]

dGTP + n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_DNA -> n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_DNA + Diphosphate		 (( (( (E * kcat) ) * B) ) / (Kb + B)) buffer: 200 mM potassium cacodylate, 8 mM MgCl2, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 35
uniprot:P04053 [DNA nucleotidylexotransferase(Enzyme) mutant D345E]

dGTP + n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_DNA -> Diphosphate + n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_DNA		 (( (( (E * kcat) ) * B) ) / (Kb + B)) buffer: 200 mM potassium cacodylate, 8 mM MgCl2, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 35
uniprot:P04053 [DNA nucleotidylexotransferase(Enzyme) mutant R336A]

n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_DNA + dGTP -> Diphosphate + n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_DNA		 (( (( (E * kcat) ) * B) ) / (Kb + B)) buffer: 200 mM potassium cacodylate, 8 mM MgCl2, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 35
uniprot:P04053 [DNA nucleotidylexotransferase(Enzyme) mutant R336A]

dGTP + n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_DNA -> Diphosphate + n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_DNA		 (( (( (E * kcat) ) * A) ) / (Ka + A)) buffer: 200 mM potassium cacodylate, 8 mM MgCl2, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 35
uniprot:P04053 [DNA nucleotidylexotransferase(Enzyme) mutant R336Q]

n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_DNA + dGTP -> n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_DNA + Diphosphate		 (( (( (E * kcat) ) * B) ) / (Kb + B)) buffer: 200 mM potassium cacodylate, 8 mM MgCl2, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 35
uniprot:P04053 [DNA nucleotidylexotransferase(Enzyme) mutant D345E]

n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_DNA + dGTP -> n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_n+1_n_DNA + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Ka + A)) buffer: 200 mM potassium cacodylate, 8 mM MgCl2, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 35

Pathways

pathway id name