EC: 2.7.7.1

nicotinamide-nucleotide adenylyltransferase (ATP:nicotinamide-nucleotide adenylyltransferase)

enzyme lambda metabolic reaction experiment
uniprot:P24518 [nicotinamide-nucleotide adenylyltransferase(Enzyme) wildtype His-tagged]

Nicotinamide_nucleotide + ATP -> Diphosphate + NAD+		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 100 mM HEPES-KOH, 115 mM Ethanol, 40 mM Semicarbazide, 3 U of Alcohol dehydrogenase
PH: 7.5
Temperature: 37
uniprot:P44308 [nicotinamide-nucleotide adenylyltransferase(Enzyme) wildtype His-tagged]

ATP + Nicotinamide_nucleotide -> Diphosphate + NAD+		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 100 mM HEPES-KOH, 115 mM Ethanol, 40 mM Semicarbazide, 3 U of Alcohol dehydrogenase
PH: 7.5
Temperature: 37
uniprot:P24518 [nicotinamide-nucleotide adenylyltransferase(Enzyme) wildtype N-terminal His-tag]

ATP + Nicotinamide_nucleotide -> NAD+ + Diphosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-Cl, 10 mM MgCl2, 1% (v/v) ethyl alcohol, 1.25 mg/ml alcohol dehydrogenase
PH: 8
Temperature: 37
uniprot:P24518 [nicotinamide-nucleotide adenylyltransferase(Enzyme) wildtype N-terminal His-tag]

Nicotinamide_nucleotide + ATP -> NAD+ + Diphosphate		 (( (Vmax * B) ) / (Km + B)) buffer: 100 mM Tris-Cl, 10 mM MgCl2, 1% (v/v) ethyl alcohol, 1.25 mg/ml alcohol dehydrogenase
PH: 8
Temperature: 37
- [nicotinamide-nucleotide adenylyltransferase(Enzyme) wildtype]

Nicotinamide_nucleotide + ATP -> Diphosphate + NAD+		 (Vmax / (1 + (Km / A))) buffer: 69 mM acetic acid, 69 mM succinic acid, 69 mM glycine, 15 mM MgCl2
PH: 4.95
Temperature: 25
- [nicotinamide-nucleotide adenylyltransferase(Enzyme) wildtype]

ATP + Nicotinamide_nucleotide -> NAD+ + Diphosphate		 (Vmax / (1 + (Km / A))) buffer: 69 mM acetic acid, 69 mM succinic acid, 69 mM glycine, 15 mM MgCl2
PH: 5.15
Temperature: 25
- [nicotinamide-nucleotide adenylyltransferase(Enzyme) wildtype]

ATP + Nicotinamide_nucleotide -> NAD+ + Diphosphate		 (Vmax / (1 + (Km / A))) buffer: 69 mM acetic acid, 69 mM succinic acid, 69 mM glycine, 15 mM MgCl2
PH: 5.85
Temperature: 25
- [nicotinamide-nucleotide adenylyltransferase(Enzyme) wildtype]

ATP + Nicotinamide_nucleotide -> NAD+ + Diphosphate		 (Vmax / (1 + (Km / A))) buffer: 69 mM acetic acid, 69 mM succinic acid, 69 mM glycine, 15 mM MgCl2
PH: 6.66
Temperature: 25
- [nicotinamide-nucleotide adenylyltransferase(Enzyme) wildtype]

Nicotinamide_nucleotide + ATP -> Diphosphate + NAD+		 (Vmax / (1 + (Km / A))) buffer: 69 mM acetic acid, 69 mM succinic acid, 69 mM glycine, 15 mM MgCl2
PH: 5.53
Temperature: 25
- [nicotinamide-nucleotide adenylyltransferase(Enzyme) wildtype]

ATP + Nicotinamide_nucleotide -> Diphosphate + NAD+		 (Vmax / (1 + (Km / A))) buffer: 69 mM acetic acid, 69 mM succinic acid, 69 mM glycine, 15 mM MgCl2
PH: 6.21
Temperature: 25
- [nicotinamide-nucleotide adenylyltransferase(Enzyme) wildtype]

ATP + Nicotinamide_nucleotide -> Diphosphate + NAD+		 (Vmax / (1 + (Km / A))) buffer: 69 mM acetic acid, 69 mM succinic acid, 69 mM glycine, 15 mM MgCl2
PH: 7.95
Temperature: 25
- [nicotinamide-nucleotide adenylyltransferase(Enzyme) wildtype]

Nicotinamide_nucleotide + ATP -> NAD+ + Diphosphate		 (Vmax / (1 + (Km / A))) buffer: 69 mM acetic acid, 69 mM succinic acid, 69 mM glycine, 15 mM MgCl2
PH: 7.16
Temperature: 25
- [nicotinamide-nucleotide adenylyltransferase(Enzyme) wildtype]

Nicotinamide_nucleotide + ATP -> Diphosphate + NAD+		 (Vmax / (1 + (Km / A))) buffer: 69 mM acetic acid, 69 mM succinic acid, 69 mM glycine, 15 mM MgCl2
PH: 7.63
Temperature: 25
- [nicotinamide-nucleotide adenylyltransferase(Enzyme) wildtype]

Nicotinamide_nucleotide + ATP -> Diphosphate + NAD+		 (Vmax / (1 + (Km / A))) buffer: 69 mM acetic acid, 69 mM succinic acid, 69 mM glycine, 15 mM MgCl2
PH: 8.76
Temperature: 25
- [nicotinamide-nucleotide adenylyltransferase(Enzyme) wildtype]

Nicotinamide_nucleotide + ATP -> NAD+ + Diphosphate		 (Vmax / (1 + (Km / A))) buffer: 69 mM acetic acid, 69 mM succinic acid, 69 mM glycine, 15 mM MgCl2
PH: 8.44
Temperature: 25
- [nicotinamide-nucleotide adenylyltransferase(Enzyme) wildtype]

ATP + Nicotinamide_nucleotide -> NAD+ + Diphosphate		 (Vmax / (1 + (( (( (Km_min * (1 + (( (10 ^ (0 - pH)) ) / (10 ^ (0 - pK1))))) ) * (1 + (( (10 ^ (0 - pK2)) ) / (10 ^ (0 - pH))))) ) / A))) buffer: 69 mM acetic acid, 69 mM succinic acid, 69 mM glycine, 15 mM MgCl2
PH: 4.95
Temperature: 25
- [nicotinamide-nucleotide adenylyltransferase(Enzyme) wildtype]

ATP + Nicotinamide_nucleotide -> Diphosphate + NAD+		 (Vmax / (1 + (Km / A))) buffer: 69 mM acetic acid, 69 mM succinic acid, 69 mM glycine, 15 mM MgCl2
PH: 9.55
Temperature: 25
- [nicotinamide-nucleotide adenylyltransferase(Enzyme) wildtype]

ATP + Nicotinamide_nucleotide -> NAD+ + Diphosphate		 (Vmax / (1 + (Km / A))) buffer: 69 mM acetic acid, 69 mM succinic acid, 69 mM glycine, 15 mM MgCl2
PH: 9.4
Temperature: 25
- [nicotinamide-nucleotide adenylyltransferase(Enzyme) wildtype]

ATP + Nicotinamide_nucleotide -> Diphosphate + NAD+		 (Vmax / (1 + (Km / A))) buffer: 69 mM acetic acid, 69 mM succinic acid, 69 mM glycine, 15 mM MgCl2
PH: 10
Temperature: 25
- [nicotinamide-nucleotide adenylyltransferase(Enzyme) wildtype]

ATP + Nicotinamide_nucleotide -> NAD+ + Diphosphate		 (Vmax / (1 + (Km / A))) buffer: 69 mM acetic acid, 69 mM succinic acid, 69 mM glycine, 15 mM MgCl2
PH: 10.35
Temperature: 25
- [nicotinamide-nucleotide adenylyltransferase(Enzyme) wildtype]

ATP + Nicotinamide_nucleotide -> NAD+ + Diphosphate		 (Vmax / (1 + (Km / A))) buffer: 69 mM acetic acid, 69 mM succinic acid, 69 mM glycine, 15 mM MgCl2
PH: 9.77
Temperature: 25
- [nicotinamide-nucleotide adenylyltransferase(Enzyme) wildtype]

ATP + Nicotinamide_nucleotide -> NAD+ + Diphosphate		 (Vmax / (1 + (Km / A))) buffer: 69 mM acetic acid, 69 mM succinic acid, 69 mM glycine, 15 mM MgCl2
PH: 10.55
Temperature: 25
uniprot:Q96T66 [nicotinamide-nucleotide adenylyltransferase(Enzyme) wildtype His-tagged NMNAT3]

Nicotinamide_nucleotide + ITP -> Nicotinamide_hypoxanthine_dinucleotide + Diphosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 20 µM MgCl2
PH: 7.5
Temperature: 30
uniprot:Q96T66 [nicotinamide-nucleotide adenylyltransferase(Enzyme) wildtype His-tagged NMNAT3]

Nicotinamide_nucleotide + GTP -> NGD+ + Diphosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 20 µM MgCl2
PH: 7.5
Temperature: 30
uniprot:Q9BZQ4 [nicotinamide-nucleotide adenylyltransferase(Enzyme) wildtype His-tagged NMNAT2]

Tiazofurin_monophosphate + ATP -> Diphosphate + Tiazofurin_adenine_dinucleotide		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 20 µM MgCl2
PH: 7.5
Temperature: 30
uniprot:Q9HAN9 [nicotinamide-nucleotide adenylyltransferase(Enzyme) wildtype His-tagged NMNAT1]

ATP + Tiazofurin_monophosphate -> Diphosphate + Tiazofurin_adenine_dinucleotide		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 2 mM ZnCl2
PH: 7.5
Temperature: 30
uniprot:Q9HAN9 [nicotinamide-nucleotide adenylyltransferase(Enzyme) wildtype His-tagged NMNAT1]

Tiazofurin_monophosphate + ATP -> Tiazofurin_adenine_dinucleotide + Diphosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 2 mM ZnCl2
PH: 7.5
Temperature: 30
uniprot:Q9HAN9 [P1-(Adenosine-5')-P4-(nicotinamide-riboside-5')-triphosphate]

ATP + Nicotinamide_nucleotide -> NAD+ + Diphosphate		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (( (I / alpha) ) * Ki))))) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 2 mM ZnCl2
PH: 7.5
Temperature: 30
uniprot:Q96T66 [P1-(Adenosine-5')-P4-(nicotinamide-riboside-5')-triphosphate]

Nicotinamide_nucleotide + ATP -> NAD+ + Diphosphate		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (( (I / alpha) ) * Ki))))) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 20 µM MgCl2
PH: 7.5
Temperature: 30
uniprot:Q96T66 [P1-(Adenosine-5')-P4-(nicotinamide-riboside-5')-triphosphate]

Nicotinamide_nucleotide + ATP -> NAD+ + Diphosphate		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (( (I / alpha) ) * Ki))))) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 20 µM MgCl2
PH: 7.5
Temperature: 30
uniprot:Q9BZQ4 [P1-(Adenosine-5')-P4-(nicotinamide-riboside-5')-triphosphate]

Nicotinamide_nucleotide + ATP -> NAD+ + Diphosphate		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (( (I / alpha) ) * Ki))))) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 20 µM MgCl2
PH: 7.5
Temperature: 30
uniprot:Q9BZQ4 [P1-(Adenosine-5')-P4-(nicotinamide-riboside-5')-triphosphate]

Nicotinamide_nucleotide + ATP -> Diphosphate + NAD+		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (( (I / alpha) ) * Ki))))) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 20 µM MgCl2
PH: 7.5
Temperature: 30
uniprot:Q9HAN9 [P1-(Adenosine-5')-P4-(nicotinamide-riboside-5')-triphosphate]

Nicotinamide_nucleotide + ATP -> Diphosphate + NAD+		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (( (I / alpha) ) * Ki))))) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 2 mM ZnCl2
PH: 7.5
Temperature: 30
uniprot:Q9HAN9 [P1-(Adenosine-5')-P4-(nicotinamide-riboside-5')-triphosphate]

Nicotinamide_nucleotide + ATP -> Diphosphate + NAD+		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (( (I / alpha) ) * Ki))))) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 2 mM ZnCl2
PH: 7.5
Temperature: 30
uniprot:Q9BZQ4 [P1-(Adenosine-5')-P4-(nicotinamide-riboside-5')-triphosphate]

ATP + Nicotinamide_nucleotide -> Diphosphate + NAD+		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (( (I / alpha) ) * Ki))))) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 20 µM MgCl2
PH: 7.5
Temperature: 30
uniprot:Q9HAN9 [P1-(Adenosine-5')-P4-(nicotinamide-riboside-5')-triphosphate]

Nicotinamide_nucleotide + ATP -> NAD+ + Diphosphate		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (( (I / alpha) ) * Ki))))) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 2 mM ZnCl2
PH: 7.5
Temperature: 30
uniprot:Q96T66 [P1-(Adenosine-5')-P3-(nicotinamide-riboside-5')-triphosphate]

ATP + Nicotinamide_nucleotide -> Diphosphate + NAD+		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (( (I / alpha) ) * Ki))))) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 20 µM MgCl2
PH: 7.5
Temperature: 30
uniprot:Q96T66 [P1-(Adenosine-5')-P3-(nicotinamide-riboside-5')-triphosphate]

Nicotinamide_nucleotide + ATP -> Diphosphate + NAD+		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (( (I / alpha) ) * Ki))))) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 20 µM MgCl2
PH: 7.5
Temperature: 30
uniprot:Q9HAN9 [P1-(Adenosine-5')-P3-(nicotinamide-riboside-5')-triphosphate]

Nicotinamide_nucleotide + ATP -> NAD+ + Diphosphate		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (( (I / alpha) ) * Ki))))) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 2 mM ZnCl2
PH: 7.5
Temperature: 30
uniprot:Q9BZQ4 [P1-(Adenosine-5')-P3-(nicotinamide-riboside-5')-triphosphate]

Nicotinamide_nucleotide + ATP -> NAD+ + Diphosphate		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (( (I / alpha) ) * Ki))))) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 20 µM MgCl2
PH: 7.5
Temperature: 30
uniprot:Q9BZQ4 [P1-(Adenosine-5')-P3-(nicotinamide-riboside-5')-triphosphate]

Nicotinamide_nucleotide + ATP -> Diphosphate + NAD+		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (( (I / alpha) ) * Ki))))) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 20 µM MgCl2
PH: 7.5
Temperature: 30
uniprot:Q9HAN9 [NAD+]

Nicotinate_D_ribonucleotide + ATP -> Diphosphate + Deamino_NAD+		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (( (I / alpha) ) * Ki))))) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 2 mM ZnCl2
PH: 7.5
Temperature: 30
uniprot:Q9HAN9 [NAD+]

Nicotinate_D_ribonucleotide + ATP -> Deamino_NAD+ + Diphosphate		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (( (I / alpha) ) * Ki))))) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 2 mM ZnCl2
PH: 7.5
Temperature: 30
uniprot:Q9BZQ4 [NAD+]

Nicotinate_D_ribonucleotide + ATP -> Deamino_NAD+ + Diphosphate		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (( (I / alpha) ) * Ki))))) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 20 µM MgCl2
PH: 7.5
Temperature: 30
uniprot:Q9BZQ4 [NAD+]

ATP + Nicotinate_D_ribonucleotide -> Deamino_NAD+ + Diphosphate		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (( (I / alpha) ) * Ki))))) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 20 µM MgCl2
PH: 7.5
Temperature: 30
uniprot:Q96T66 [Deamino-NAD+]

ATP + Nicotinamide_nucleotide -> NAD+ + Diphosphate		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (( (I / alpha) ) * Ki))))) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 20 µM MgCl2
PH: 7.5
Temperature: 30
uniprot:Q96T66 [Deamino-NAD+]

Nicotinamide_nucleotide + ATP -> NAD+ + Diphosphate		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (( (I / alpha) ) * Ki))))) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 20 µM MgCl2
PH: 7.5
Temperature: 30
uniprot:Q9BZQ4 [Deamino-NAD+]

ATP + Nicotinamide_nucleotide -> Diphosphate + NAD+		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (( (I / alpha) ) * Ki))))) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 20 µM MgCl2
PH: 7.5
Temperature: 30
uniprot:Q9HAN9 [P1-(Adenosine-5')-P3-(nicotinamide-riboside-5')-triphosphate]

ATP + Nicotinamide_nucleotide -> NAD+ + Diphosphate		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (( (I / alpha) ) * Ki))))) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 2 mM ZnCl2
PH: 7.5
Temperature: 30
uniprot:Q9HAN9 [Deamino-NAD+]

Nicotinamide_nucleotide + ATP -> NAD+ + Diphosphate		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (( (I / alpha) ) * Ki))))) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 2 mM ZnCl2
PH: 7.5
Temperature: 30
uniprot:Q9HAN9 [Deamino-NAD+]

ATP + Nicotinamide_nucleotide -> NAD+ + Diphosphate		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (( (I / alpha) ) * Ki))))) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 2 mM ZnCl2
PH: 7.5
Temperature: 30
uniprot:Q9BZQ4 [Deamino-NAD+]

Nicotinamide_nucleotide + ATP -> Diphosphate + NAD+		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (( (I / alpha) ) * Ki))))) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 20 µM MgCl2
PH: 7.5
Temperature: 30
uniprot:Q9HAN9 [nicotinamide-nucleotide adenylyltransferase(Enzyme) wildtype His-tagged NMNAT1]

Nicotinamide_nucleotide + ATP -> NAD+ + Diphosphate		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (( (I / alpha) ) * Ki))))) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 2 mM ZnCl2
PH: 7.5
Temperature: 30
uniprot:Q96T66 [nicotinamide-nucleotide adenylyltransferase(Enzyme) wildtype His-tagged NMNAT3]

Nicotinamide_nucleotide + ATP -> Diphosphate + NAD+		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (( (I / alpha) ) * Ki))))) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 20 µM MgCl2
PH: 7.5
Temperature: 30
uniprot:Q9HAN9 [nicotinamide-nucleotide adenylyltransferase(Enzyme) wildtype His-tagged NMNAT1]

Nicotinamide_nucleotide + ATP -> Diphosphate + NAD+		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (( (I / alpha) ) * Ki))))) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 2 mM ZnCl2
PH: 7.5
Temperature: 30
uniprot:Q96T66 [nicotinamide-nucleotide adenylyltransferase(Enzyme) wildtype His-tagged NMNAT3]

ATP + Nicotinamide_nucleotide -> Diphosphate + NAD+		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (( (I / alpha) ) * Ki))))) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 20 µM MgCl2
PH: 7.5
Temperature: 30
uniprot:Q9BZQ4 [nicotinamide-nucleotide adenylyltransferase(Enzyme) wildtype His-tagged NMNAT2]

Nicotinamide_nucleotide + ATP -> Diphosphate + NAD+		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (( (I / alpha) ) * Ki))))) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 20 µM MgCl2
PH: 7.5
Temperature: 30
uniprot:Q9BZQ4 [nicotinamide-nucleotide adenylyltransferase(Enzyme) wildtype His-tagged NMNAT2]

ATP + Nicotinamide_nucleotide -> NAD+ + Diphosphate		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (( (I / alpha) ) * Ki))))) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 20 µM MgCl2
PH: 7.5
Temperature: 30
uniprot:Q96T66 [P1-(Adenosine-5')-P4-(nicotinamide-riboside-5')-triphosphate]

Nicotinamide_nucleotide + ATP -> NAD+ + Diphosphate		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (( (I / alpha) ) * Ki))))) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 20 µM MgCl2
PH: 7.5
Temperature: 30
uniprot:Q96T66 [P1-(Adenosine-5')-P4-(nicotinamide-riboside-5')-triphosphate]

ATP + Nicotinamide_nucleotide -> Diphosphate + NAD+		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (( (I / alpha) ) * Ki))))) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 20 µM MgCl2
PH: 7.5
Temperature: 30
uniprot:Q9BZQ4 [P1-(Adenosine-5')-P4-(nicotinamide-riboside-5')-triphosphate]

ATP + Nicotinamide_nucleotide -> NAD+ + Diphosphate		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (( (I / alpha) ) * Ki))))) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 20 µM MgCl2
PH: 7.5
Temperature: 30
uniprot:Q96T66 [nicotinamide-nucleotide adenylyltransferase(Enzyme) wildtype His-tagged NMNAT3]

ATP + Nicotinamide_nucleotide -> NAD+ + Diphosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 20 mM MgCl2
PH: 7.5
Temperature: 30
uniprot:Q96T66 [nicotinamide-nucleotide adenylyltransferase(Enzyme) wildtype His-tagged NMNAT3]

ATP + Nicotinamide_nucleotide -> NAD+ + Diphosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 20 mM MgCl2
PH: 7.5
Temperature: 30
uniprot:Q9HAN9 [nicotinamide-nucleotide adenylyltransferase(Enzyme) wildtype His-tagged NMNAT1]

Nicotinamide_nucleotide + ATP -> Diphosphate + NAD+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 2 mM ZnCl2
PH: 7.5
Temperature: 30
uniprot:Q9HAN9 [nicotinamide-nucleotide adenylyltransferase(Enzyme) wildtype His-tagged NMNAT1]

ATP + Nicotinamide_nucleotide -> NAD+ + Diphosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 2 mM ZnCl2
PH: 7.5
Temperature: 30
uniprot:Q9BZQ4 [nicotinamide-nucleotide adenylyltransferase(Enzyme) wildtype His-tagged NMNAT2]

ATP + Nicotinamide_nucleotide -> Diphosphate + NAD+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 20 µM MgCl2
PH: 7.5
Temperature: 30
uniprot:Q9BZQ4 [nicotinamide-nucleotide adenylyltransferase(Enzyme) wildtype His-tagged NMNAT2]

Nicotinamide_nucleotide + ATP -> NAD+ + Diphosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 20 µM MgCl2
PH: 7.5
Temperature: 30

Pathways

pathway id name
BioCyc:HUMAN_NAD-BIOSYNTHESIS-III NAD salvage
BioCyc:HUMAN_PWY66-401 superpathway of tryptophan utilization
BioCyc:META_PWY-3502 superpathway of NAD biosynthesis in eukaryotes
BioCyc:META_PWY3O-4107 NAD salvage pathway V (PNC V cycle)
BioCyc:YEAST_PWY3O-4107 NAD salvage pathway
BioCyc:META_NAD-BIOSYNTHESIS-III NAD biosynthesis III (from nicotinamide)
BioCyc:YEAST_PWY-5653 NAD biosynthesis from 2-amino-3-carboxymuconate semialdehyde
BioCyc:MOUSE_NADSYN-PWY NAD biosynthesis II (from tryptophan)
BioCyc:ECOL316407_PWY3O-4106 NAD salvage pathway III
BioCyc:META_NADSYN-PWY NAD de novo biosynthesis II (from tryptophan)
BioCyc:YEAST_NADSYN-PWY NAD de novo biosynthesis
BioCyc:MOUSE_PWY3O-4106 NAD salvage pathway III
BioCyc:MOUSE_NAD-BIOSYNTHESIS-III NAD biosynthesis III
BioCyc:HUMAN_NADSYN-PWY NAD de novo biosynthesis
BioCyc:HUMAN_PWY-5653 NAD biosynthesis from 2-amino-3-carboxymuconate semialdehyde
BioCyc:META_PWY3O-4106 NAD salvage pathway IV (from nicotinamide riboside)
BioCyc:META_PWY-5653 NAD biosynthesis from 2-amino-3-carboxymuconate semialdehyde
BioCyc:ECO_PWY3O-4106 NAD salvage pathway IV (from nicotinamide riboside)
BioCyc:YEAST_PWY3O-4106 nicotinamide riboside salvage pathway I
BioCyc:MOUSE_PWY-5653 NAD biosynthesis from 2-amino-3-carboxymuconate semialdehyde
BioCyc:MOUSE_NAD-BIOSYNTHESIS-II NAD salvage pathway II
BioCyc:MOUSE_PYRNUCYC-PWY pyridine nucleotide cycling
PathBank:SMP0000850 NAD Salvage
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-196807 Nicotinate metabolism
Reactome:R-DRE-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-DME-196807 Nicotinate metabolism
Reactome:R-HSA-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-RNO-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-SPO-1430728 Metabolism
Reactome:R-BTA-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-DRE-196854 Metabolism of vitamins and cofactors
Reactome:R-DME-1430728 Metabolism
Reactome:R-DME-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-HSA-196854 Metabolism of vitamins and cofactors
Reactome:R-MMU-196854 Metabolism of vitamins and cofactors
Reactome:R-RNO-196854 Metabolism of vitamins and cofactors
Reactome:R-SPO-196807 Nicotinate metabolism
Reactome:R-DRE-1430728 Metabolism
Reactome:R-DRE-196807 Nicotinate metabolism
Reactome:R-HSA-196807 Nicotinate metabolism
Reactome:R-MMU-196807 Nicotinate metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-196807 Nicotinate metabolism
Reactome:R-SPO-196854 Metabolism of vitamins and cofactors
Reactome:R-BTA-196854 Metabolism of vitamins and cofactors
Reactome:R-DME-196854 Metabolism of vitamins and cofactors
Reactome:R-HSA-1430728 Metabolism
Reactome:R-SPO-196849 Metabolism of water-soluble vitamins and cofactors
PathBank:SMP0083297 NAD+ Signalling Pathway (Cancer)
PathBank:SMP0084271 NAD+ Signalling and Aging
PathBank:SMP0087334 Nicotinate and Nicotinamide Metabolism
PathBank:SMP0087241 Nicotinate and Nicotinamide Metabolism
PathBank:SMP0002338 NAD Metabolism
PathBank:SMP0087456 Nicotinate and Nicotinamide Metabolism
PathBank:SMP0000048 Nicotinate and Nicotinamide Metabolism