EC: 2.7.6.3

2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase (ATP:6-hydroxymethyl-7,8-dihydropterin 6'-diphosphotransferase)

enzyme lambda metabolic reaction experiment
uniprot:Q1ENB6 [Enzyme wildtype His-tagged (N-term)]

2_Amino_4_hydroxy_6_hydroxymethyl_7,8_dihydropteridine + ATP + 4_Aminobenzoate -> Dihydropteroate + Diphosphate + AMP		 (( (Vmax * A) ) / (Km + A)) buffer: 40 mM Tris, 20 mM beta-mercaptoethanol, 10 mM MgCl2
PH: 8
Temperature: 30
uniprot:Q1ENB6 [Enzyme wildtype His-tagged (N-term)]

ATP + 4_Aminobenzoate + 2_Amino_4_hydroxy_6_hydroxymethyl_7,8_dihydropteridine -> Dihydropteroate + AMP + Diphosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 40 mM Tris, 20 mM beta-mercaptoethanol, 10 mM MgCl2
PH: 8
Temperature: 30
uniprot:Q1ENB6 [Enzyme wildtype His-tagged (N-term)]

ATP + 4_Aminobenzoate + 2_Amino_4_hydroxy_6_hydroxymethyl_7,8_dihydropteridine -> Dihydropteroate + Diphosphate + AMP		 (( (Vmax * A) ) / (Km + A)) buffer: 40 mM Tris, 20 mM beta-mercaptoethanol, 10 mM MgCl2
PH: 8
Temperature: 30
uniprot:O04862 [Enzyme wildtype]

2_Amino_4_hydroxy_6_hydroxymethyl_7,8_dihydropteridine + ATP + 4_Aminobenzoate -> Diphosphate + Dihydropteroate + AMP		 (( (Vmax * A) ) / (Km + A)) buffer: 40 mM Tris, 20 mM beta-mercaptoethanol, 10 mM MgCl2
PH: 8
Temperature: 30
uniprot:O04862 [Enzyme wildtype]

ATP + 4_Aminobenzoate + 2_Amino_4_hydroxy_6_hydroxymethyl_7,8_dihydropteridine -> Diphosphate + Dihydropteroate + AMP		 (( (Vmax * C) ) / (Km + C)) buffer: 40 mM Tris, 20 mM beta-mercaptoethanol, 10 mM MgCl2
PH: 8
Temperature: 30
uniprot:O04862 [Enzyme wildtype]

4_Aminobenzoate + 2_Amino_4_hydroxy_6_hydroxymethyl_7,8_dihydropteridine + ATP -> Dihydropteroate + AMP + Diphosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 40 mM Tris, 20 mM beta-mercaptoethanol, 10 mM MgCl2
PH: 8
Temperature: 30
uniprot:O04862 [Enzyme wildtype]

2_Amino_4_hydroxy_6_hydroxymethyl_7,8_dihydropteridine + ATP + 4_Aminobenzoate -> Dihydropteroate + AMP + Diphosphate		 (( (Vmax * C) ) / (Km + C)) buffer: 40 mM Tris, 20 mM beta-mercaptoethanol, 10 mM MgCl2
PH: 8
Temperature: 30

Pathways

pathway id name
BioCyc:META_PWY-6797 6-hydroxymethyl-dihydropterin diphosphate biosynthesis II (Methanocaldococcus)
BioCyc:META_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:ECO_PWY-6612 superpathway of tetrahydrofolate biosynthesis
BioCyc:YEAST_FOLSYN-PWY superpathway of tetrahydrofolate biosynthesis and salvage
BioCyc:ARA_PWY-6612 superpathway of tetrahydrofolate biosynthesis
BioCyc:ARA_PWY-6147 6-hydroxymethyl-dihydropterin diphosphate biosynthesis I
BioCyc:MTBH37RV_PWY-6147 6-hydroxymethyl-dihydropterin diphosphate biosynthesis I
BioCyc:MTBH37RV_PWY-6612 superpathway of tetrahydrofolate biosynthesis
BioCyc:ECOL316407_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:META_PWY-6612 superpathway of tetrahydrofolate biosynthesis
BioCyc:YEAST_PWY-6147 6-hydroxymethyl-dihydropterin diphosphate biosynthesis I
BioCyc:ARA_PWY-3742 tetrahydrofolate biosynthesis II
BioCyc:MTBH37RV_FOLSYN-PWY superpathway of tetrahydrofolate biosynthesis and salvage
BioCyc:MTBCDC1551_PWY-6147 6-hydroxymethyl-dihydropterin diphosphate biosynthesis I
BioCyc:BSUB_PWY-6147 6-hydroxymethyl-dihydropterin diphosphate biosynthesis I
BioCyc:BSUB_PWY-6612 superpathway of tetrahydrofolate biosynthesis
BioCyc:ECOL316407_PWY-6612 superpathway of tetrahydrofolate biosynthesis
BioCyc:META_PWY-7539 6-hydroxymethyl-dihydropterin diphosphate biosynthesis III (Chlamydia)
BioCyc:META_PWY-6147 6-hydroxymethyl-dihydropterin diphosphate biosynthesis I
BioCyc:META_PWY-6148 tetrahydromethanopterin biosynthesis
BioCyc:YEAST_PWY-6612 superpathway of tetrahydrofolate biosynthesis
BioCyc:META_FOLSYN-PWY superpathway of tetrahydrofolate biosynthesis and salvage
BioCyc:ECO_PWY-6147 6-hydroxymethyl-dihydropterin diphosphate biosynthesis I
BioCyc:ECO_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:YEAST_PWY-6614 tetrahydrofolate biosynthesis
BioCyc:ARA_PWY-6614 tetrahydrofolate biosynthesis
BioCyc:MTBCDC1551_PWY-6612 superpathway of tetrahydrofolate biosynthesis
BioCyc:MTBCDC1551_FOLSYN-PWY superpathway of tetrahydrofolate biosynthesis and salvage
BioCyc:BSUB_FOLSYN-PWY superpathway of tetrahydrofolate biosynthesis and salvage
BioCyc:ECOL316407_PWY-6147 6-hydroxymethyl-dihydropterin diphosphate biosynthesis I
PathBank:SMP0000925 Folate Biosynthesis
PathBank:SMP0001902 GTP Degradation and Molybdenum Cofactor Biosynthesis
PlantCyc:ARA_PWY-3742 tetrahydrofolate biosynthesis II
PlantCyc:ARA_PWY-6147 6-hydroxymethyl-dihydropterin diphosphate biosynthesis I
PlantCyc:PLANT_PWY-6614 tetrahydrofolate biosynthesis
PlantCyc:PLANT_PWY-6147 6-hydroxymethyl-dihydropterin diphosphate biosynthesis I
PlantCyc:ARA_PWY-6612 superpathway of tetrahydrofolate biosynthesis
PlantCyc:PLANT_PWY-3742 tetrahydrofolate biosynthesis II
PlantCyc:ARA_PWY-6614 tetrahydrofolate biosynthesis
PlantCyc:PLANT_PWY-6612 superpathway of tetrahydrofolate biosynthesis
PathBank:SMP0012014 Folate Biosynthesis
PathBank:SMP0012013 Pterin Biosynthesis (Folate Precursor)
PathBank:SMP0002334 Tetrahydrofolate Biosynthesis