EC: 2.7.4.25

(d)CMP kinase (ATP:(d)CMP phosphotransferase)

enzyme lambda metabolic reaction experiment
uniprot:P0A6I0 [(d)CMP kinase(Enzyme) mutant R110M]

dCMP + ATP -> dCDP + ADP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris ⁄HCl, 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, 4 U/ml pyruvate kinase, 4 U/ml lactate dehydrogenase, 4 U/ml NDP kinase
PH: 7.4
Temperature: 30
uniprot:P0A6I0 [(d)CMP kinase(Enzyme) mutant D132H]

ATP + dCMP -> ADP + dCDP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris ⁄HCl, 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, 4 U/ml pyruvate kinase, 4 U/ml lactate dehydrogenase, 4 U/ml NDP kinase
PH: 7.4
Temperature: 30
uniprot:P0A6I0 [(d)CMP kinase(Enzyme) mutant R188M]

ATP + dCMP -> dCDP + ADP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris ⁄HCl, 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, 4 U/ml pyruvate kinase, 4 U/ml lactate dehydrogenase, 4 U/ml NDP kinase
PH: 7.4
Temperature: 30
uniprot:P0A6I0 [(d)CMP kinase(Enzyme) mutant D132H]

ATP + CMP -> ADP + CDP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris ⁄HCl, 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, 4 U/ml pyruvate kinase, 4 U/ml lactate dehydrogenase, 4 U/ml NDP kinase
PH: 7.4
Temperature: 30
uniprot:P0A6I0 [(d)CMP kinase(Enzyme) mutant D132S]

ATP + CMP -> CDP + ADP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris ⁄HCl, 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, 4 U/ml pyruvate kinase, 4 U/ml lactate dehydrogenase, 4 U/ml NDP kinase
PH: 7.4
Temperature: 30
uniprot:P0A6I0 [(d)CMP kinase(Enzyme) mutant D132N]

ATP + CMP -> CDP + ADP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris ⁄HCl, 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, 4 U/ml pyruvate kinase, 4 U/ml lactate dehydrogenase, 4 U/ml NDP kinase
PH: 7.4
Temperature: 30
uniprot:P0A6I0 [(d)CMP kinase(Enzyme) mutant D132A]

ATP + CMP -> ADP + CDP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris ⁄HCl, 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, 4 U/ml pyruvate kinase, 4 U/ml lactate dehydrogenase, 4 U/ml NDP kinase
PH: 7.4
Temperature: 30
uniprot:P0A6I0 [(d)CMP kinase(Enzyme) mutant R110M]

CMP + ATP -> CDP + ADP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris ⁄HCl, 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, 4 U/ml pyruvate kinase, 4 U/ml lactate dehydrogenase, 4 U/ml NDP kinase
PH: 7.4
Temperature: 30
uniprot:P0A6I0 [(d)CMP kinase(Enzyme) mutant R188M]

ATP + CMP -> ADP + CDP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris ⁄HCl, 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, 4 U/ml pyruvate kinase, 4 U/ml lactate dehydrogenase, 4 U/ml NDP kinase
PH: 7.4
Temperature: 30
uniprot:P0A6I0 [(d)CMP kinase(Enzyme) wildtype]

ATP + dCMP -> ADP + dCDP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris ⁄HCl, 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, 4 U/ml pyruvate kinase, 4 U/ml lactate dehydrogenase, 4 U/ml NDP kinase
PH: 7.4
Temperature: 30
uniprot:P0A6I0 [(d)CMP kinase(Enzyme) mutant D132N]

dCMP + ATP -> ADP + dCDP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris ⁄HCl, 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, 4 U/ml pyruvate kinase, 4 U/ml lactate dehydrogenase, 4 U/ml NDP kinase
PH: 7.4
Temperature: 30
uniprot:P0A6I0 [(d)CMP kinase(Enzyme) mutant D132S]

ATP + dCMP -> ADP + dCDP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris ⁄HCl, 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, 4 U/ml pyruvate kinase, 4 U/ml lactate dehydrogenase, 4 U/ml NDP kinase
PH: 7.4
Temperature: 30
uniprot:P0A6I0 [(d)CMP kinase(Enzyme) wildtype]

UMP + ATP -> UDP + ADP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris ⁄HCl, 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, 4 U/ml pyruvate kinase, 4 U/ml lactate dehydrogenase, 4 U/ml NDP kinase
PH: 7.4
Temperature: 30
uniprot:P0A6I0 [(d)CMP kinase(Enzyme) mutant D132A]

ATP + dCMP -> ADP + dCDP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris ⁄HCl, 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, 4 U/ml pyruvate kinase, 4 U/ml lactate dehydrogenase, 4 U/ml NDP kinase
PH: 7.4
Temperature: 30
uniprot:P0A6I0 [(d)CMP kinase(Enzyme) mutant S36A]

ATP + dCMP -> ADP + dCDP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris ⁄HCl, 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, 4 U/ml pyruvate kinase, 4 U/ml lactate dehydrogenase, 4 U/ml NDP kinase
PH: 7.4
Temperature: 30
uniprot:P0A6I0 [(d)CMP kinase(Enzyme) mutant S36A]

UMP + ATP -> UDP + ADP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris ⁄HCl, 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, 4 U/ml pyruvate kinase, 4 U/ml lactate dehydrogenase, 4 U/ml NDP kinase
PH: 7.4
Temperature: 30
uniprot:P0A6I0 [(d)CMP kinase(Enzyme) mutant D132S]

UMP + ATP -> ADP + UDP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris ⁄HCl, 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, 4 U/ml pyruvate kinase, 4 U/ml lactate dehydrogenase, 4 U/ml NDP kinase
PH: 7.4
Temperature: 30
uniprot:P0A6I0 [(d)CMP kinase(Enzyme) mutant D132A]

UMP + ATP -> ADP + UDP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris ⁄HCl, 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, 4 U/ml pyruvate kinase, 4 U/ml lactate dehydrogenase, 4 U/ml NDP kinase
PH: 7.4
Temperature: 30
uniprot:P0A6I0 [(d)CMP kinase(Enzyme) mutant R110M]

ATP + UMP -> ADP + UDP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris ⁄HCl, 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, 4 U/ml pyruvate kinase, 4 U/ml lactate dehydrogenase, 4 U/ml NDP kinase
PH: 7.4
Temperature: 30
uniprot:P0A6I0 [(d)CMP kinase(Enzyme) mutant D132H]

UMP + ATP -> UDP + ADP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris ⁄HCl, 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, 4 U/ml pyruvate kinase, 4 U/ml lactate dehydrogenase, 4 U/ml NDP kinase
PH: 7.4
Temperature: 30
uniprot:P0A6I0 [(d)CMP kinase(Enzyme) mutant D132N]

UMP + ATP -> UDP + ADP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris ⁄HCl, 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, 4 U/ml pyruvate kinase, 4 U/ml lactate dehydrogenase, 4 U/ml NDP kinase
PH: 7.4
Temperature: 30
uniprot:P0A6I0 [(d)CMP kinase(Enzyme) mutant S36A]

ATP + CMP -> CDP + ADP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris ⁄HCl, 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, 4 U/ml pyruvate kinase, 4 U/ml lactate dehydrogenase, 4 U/ml NDP kinase
PH: 7.4
Temperature: 30
uniprot:P0A6I0 [(d)CMP kinase(Enzyme) wildtype]

ATP + CMP -> ADP + CDP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris ⁄HCl, 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, 4 U/ml pyruvate kinase, 4 U/ml lactate dehydrogenase, 4 U/ml NDP kinase
PH: 7.4
Temperature: 30

Pathways

pathway id name
BioCyc:META_PWY-7205 CMP phosphorylation
BioCyc:ECOO157_PWY-7197 pyrimidine deoxyribonucleotide phosphorylation
BioCyc:ECOL199310_PWY-7200 superpathway of pyrimidine deoxyribonucleoside salvage
BioCyc:CAULO_PWY-7197 pyrimidine deoxyribonucleotide phosphorylation
BioCyc:CAULO_PWY-7205 CMP phosphorylation
BioCyc:ANTHRA_PWY-7176 UTP and CTP de novo biosynthesis
BioCyc:ANTHRA_PWY-7200 superpathway of pyrimidine deoxyribonucleoside salvage
BioCyc:SYNEL_PWY-7176 UTP and CTP de novo biosynthesis
BioCyc:SYNEL_PANTO-PWY phosphopantothenate biosynthesis I
BioCyc:CAULONA1000_P1-PWY salvage pathways of purine and pyrimidine nucleotides
BioCyc:PDIF272563_PWY-7205 CMP phosphorylation
BioCyc:BTHE_PWY0-162 superpathway of pyrimidine ribonucleotides de novo biosynthesis
BioCyc:BTHE_PWY-7176 UTP and CTP de novo biosynthesis
BioCyc:BTHE_PWY-7208 superpathway of pyrimidine nucleobases salvage
BioCyc:CORYNE_PWY-7205 CMP phosphorylation
BioCyc:CORYNE_PWY0-162 superpathway of pyrimidine ribonucleotides de novo biosynthesis
BioCyc:META_PWY-7196 superpathway of pyrimidine ribonucleosides salvage
BioCyc:ECO_PWY-7197 pyrimidine deoxyribonucleotide phosphorylation
BioCyc:ECO_PWY-7196 superpathway of pyrimidine ribonucleosides salvage
BioCyc:MTBH37RV_PWY-7208 superpathway of pyrimidine nucleobases salvage
BioCyc:MTBH37RV_PWY-7200 superpathway of pyrimidine deoxyribonucleoside salvage
BioCyc:MTBH37RV_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:MTBH37RV_PWY-7176 UTP and CTP de novo biosynthesis
BioCyc:MTBCDC1551_PWY-7200 superpathway of pyrimidine deoxyribonucleoside salvage
BioCyc:MTBCDC1551_PWY-7208 superpathway of pyrimidine nucleobases salvage
BioCyc:MTBCDC1551_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:ECOO157_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:ECOO157_PWY0-162 superpathway of pyrimidine ribonucleotides de novo biosynthesis
BioCyc:ECOL199310_PWY-7205 CMP phosphorylation
BioCyc:ECOL199310_PWY-7176 UTP and CTP de novo biosynthesis
BioCyc:ECOL199310_PWY0-162 superpathway of pyrimidine ribonucleotides de novo biosynthesis
BioCyc:ECOL199310_PWY-7197 pyrimidine deoxyribonucleotide phosphorylation
BioCyc:CAULO_PWY-7200 superpathway of pyrimidine deoxyribonucleoside salvage
BioCyc:BSUB_P1-PWY purine and pyrimidine metabolism
BioCyc:BSUB_PWY-5687 pyrimidine ribonucleotides interconversion
BioCyc:CORYNE_PWY-7176 UTP and CTP de novo biosynthesis
BioCyc:CORYNE_PWY-7197 pyrimidine deoxyribonucleotide phosphorylation
BioCyc:CORYNE_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:META_PWY-7197 pyrimidine deoxyribonucleotide phosphorylation
BioCyc:ECO_PWY-7205 CMP phosphorylation
BioCyc:MTBH37RV_PWY-7205 CMP phosphorylation
BioCyc:MTBH37RV_PWY0-162 superpathway of pyrimidine ribonucleotides de novo biosynthesis
BioCyc:MTBH37RV_PWY-7197 pyrimidine deoxyribonucleotide phosphorylation
BioCyc:MTBCDC1551_PWY-7205 CMP phosphorylation
BioCyc:MTBCDC1551_PWY-7176 UTP and CTP de novo biosynthesis
BioCyc:MTBCDC1551_PWY0-162 superpathway of pyrimidine ribonucleotides de novo biosynthesis
BioCyc:MTBCDC1551_PWY-7197 pyrimidine deoxyribonucleotide phosphorylation
BioCyc:ECOO157_PWY-7205 CMP phosphorylation
BioCyc:ECOO157_PWY-7176 UTP and CTP de novo biosynthesis
BioCyc:CAULO_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:CAULO_PWY0-162 superpathway of pyrimidine ribonucleotides de novo biosynthesis
BioCyc:CAULO_PWY-7208 superpathway of pyrimidine nucleobases salvage
BioCyc:ANTHRA_PWY-7197 pyrimidine deoxyribonucleotide phosphorylation
BioCyc:ANTHRA_PWY-7208 superpathway of pyrimidine nucleobases salvage
BioCyc:AGRO_PWY-7205 CMP phosphorylation
BioCyc:SYNEL_PWY-7197 pyrimidine deoxyribonucleotide phosphorylation
BioCyc:ECOL316407_PWY0-163 salvage pathways of pyrimidine ribonucleotides
BioCyc:CAULONA1000_PWY-5687 pyrimidine ribonucleotides interconversion
BioCyc:CAULONA1000_PWY0-162 pyrimidine ribonucleotides de novo biosynthesis
BioCyc:BTHE_PWY-7200 superpathway of pyrimidine deoxyribonucleoside salvage
BioCyc:BTHE_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:BTHE_PWY-7205 CMP phosphorylation
BioCyc:ECO_PWY0-163 salvage pathways of pyrimidine ribonucleotides
BioCyc:ECOO157_PWY-7208 superpathway of pyrimidine nucleobases salvage
BioCyc:ECOL199310_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:ECOL199310_PWY-7208 superpathway of pyrimidine nucleobases salvage
BioCyc:CAULO_PWY-7176 UTP and CTP de novo biosynthesis
BioCyc:ANTHRA_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:ANTHRA_PWY0-162 superpathway of pyrimidine ribonucleotides de novo biosynthesis
BioCyc:ANTHRA_PWY-7205 CMP phosphorylation
BioCyc:BSUB_PWY0-163 salvage pathways of pyrimidine ribonucleotides
BioCyc:SYNEL_PWY0-162 superpathway of pyrimidine ribonucleotides de novo biosynthesis
BioCyc:SYNEL_PWY-7208 superpathway of pyrimidine nucleobases salvage
BioCyc:SYNEL_PWY-7205 CMP phosphorylation
BioCyc:CAULONA1000_PWY0-163 salvage pathways of pyrimidine ribonucleotides
BioCyc:PDIF272563_PWY-7197 pyrimidine deoxyribonucleotide phosphorylation
BioCyc:BTHE_PWY-7197 pyrimidine deoxyribonucleotide phosphorylation
BioCyc:CORYNE_PWY-7208 superpathway of pyrimidine nucleobases salvage