EC: 2.7.11.18

myosin-light-chain kinase (ATP:[myosin light chain] O-phosphotransferase)

enzyme lambda metabolic reaction experiment
uniprot:Q15746 [Ca2+]

Myosin_light_chain + ATP -> Myosin_light_chain_phosphate + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM MOPS, 10 mM Mg2+ acetate, 0.025% 2-mercaptoethanol
PH: 7.4
Temperature: 22
uniprot:Q15746 [Ca2+]

Myosin_light_chain + ATP -> ADP + Myosin_light_chain_phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM MOPS, 10 mM Mg2+ acetate, 0.025% 2-mercaptoethanol
PH: 7.4
Temperature: 22
uniprot:P29294 [Ca2+]

ATP + Myosin_light_chain -> ADP + Myosin_light_chain_phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM MOPS, 10 mM Mg2+ acetate, 0.025% 2-mercaptoethanol
PH: 7.4
Temperature: 22
uniprot:Q3UIZ8 [Ca2+]

ATP + Myosin_light_chain -> Myosin_light_chain_phosphate + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 25 mM HEPES, 10 mM MgCl2, 5 mM DTT, 20 mM NaCl, 0.2% triton, 2% glycerol, 0.5 mg/ml BSA
PH: 7.6
Temperature: 30
uniprot:Q3UIZ8 [EGTA]

Myosin_light_chain + ATP -> ADP + Myosin_light_chain_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 25 mM HEPES, 10 mM MgCl2, 5 mM DTT, 20 mM NaCl, 0.2% triton, 2% glycerol, 0.5 mg/ml BSA
PH: 7.6
Temperature: 30
uniprot:Q3UIZ8 [myosin-light-chain kinase(Enzyme) wildtype HA-tagged]

Myosin_light_chain + ATP -> Myosin_light_chain_phosphate + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 25 mM HEPES, 10 mM MgCl2, 5 mM DTT, 20 mM NaCl, 0.2% triton, 2% glycerol, 0.5 mg/ml BSA
PH: 7.6
Temperature: 30
uniprot:Q15746 [Calmodulin]

ATP + Myosin_light_chain -> ADP + Myosin_light_chain_phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: *
PH: 7
Temperature: 36
uniprot:Q15746 [Calmodulin]

Myosin_light_chain + ATP -> Myosin_light_chain_phosphate + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: *
PH: 7
Temperature: 36
uniprot:Q15746 [myosin-light-chain kinase(Enzyme) mutant A1491S N-terminal FLAG-tagged]

Myosin_light_chain + ATP -> ADP + Myosin_light_chain_phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: *
PH: 7
Temperature: 36
uniprot:Q15746 [myosin-light-chain kinase(Enzyme) wildtype N-terminal FLAG-tagged]

Myosin_light_chain + ATP -> Myosin_light_chain_phosphate + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: *
PH: 7
Temperature: 36

Pathways

pathway id name
Reactome:R-HSA-9716542 Signaling by Rho GTPases, Miro GTPases and RHOBTB3
Reactome:R-MMU-9716542 Signaling by Rho GTPases, Miro GTPases and RHOBTB3
Reactome:R-BTA-9716542 Signaling by Rho GTPases, Miro GTPases and RHOBTB3
Reactome:R-HSA-445355 Smooth Muscle Contraction
Reactome:R-MMU-445355 Smooth Muscle Contraction
Reactome:R-MMU-162582 Signaling Pathways
Reactome:R-BTA-445355 Smooth Muscle Contraction
Reactome:R-BTA-162582 Signaling Pathways
Reactome:R-BTA-195258 RHO GTPase Effectors
Reactome:R-HSA-195258 RHO GTPase Effectors
Reactome:R-MMU-195258 RHO GTPase Effectors
Reactome:R-BTA-397014 Muscle contraction
Reactome:R-BTA-194315 Signaling by Rho GTPases
Reactome:R-BTA-5627123 RHO GTPases activate PAKs
Reactome:R-HSA-194315 Signaling by Rho GTPases
Reactome:R-HSA-5627123 RHO GTPases activate PAKs
Reactome:R-MMU-194315 Signaling by Rho GTPases
Reactome:R-MMU-5627123 RHO GTPases activate PAKs
Reactome:R-HSA-162582 Signaling Pathways
Reactome:R-HSA-397014 Muscle contraction
Reactome:R-MMU-397014 Muscle contraction
PathBank:SMP0119378 Muscle/Heart Contraction
PathBank:SMP0120885 Rac 1 Cell Motility Signaling Pathway
PathBank:SMP0000354 Intracellular Signalling Through Prostacyclin Receptor and Prostacyclin
PathBank:SMP0100484 Rac 1 cell motility signaling pathway
PathBank:SMP0100499 Rac 1 cell motility signaling pathway
PathBank:SMP0107998 Intracellular Signalling Through Prostacyclin Receptor and Prostacyclin
PathBank:SMP0063795 Rac 1 Cell Motility Signaling Pathway
PathBank:SMP0108008 Intracellular Signalling Through Prostacyclin Receptor and Prostacyclin
PathBank:SMP0119395 Muscle/Heart Contraction
PathBank:SMP0120887 Rac 1 Cell Motility Signaling Pathway