EC: 2.7.1.78

polynucleotide 5'-hydroxyl-kinase (ATP:5'-dephosphopolynucleotide 5'-phosphotransferase)

enzyme lambda metabolic reaction experiment
uniprot:P06855 [polynucleotide 5'-hydroxyl-kinase(Enzyme) wildtype]

ATP + 5'_Dephospho_DNA -> 5'_Phospho_DNA + ADP		 (( (Vmax * A) ) / (KmA + A)) buffer: 50 mM Tris-HCl, 10 mM MgCl2, 5 mM Dithiothreitol, 0.08 g/l bovine serum albumin
PH: 7.6
Temperature: 37
uniprot:P06855 [polynucleotide 5'-hydroxyl-kinase(Enzyme) mutant pseT 1]

5'_Dephospho_DNA + ATP -> ADP + 5'_Phospho_DNA		 (( (Vmax * A) ) / (KmA + A)) buffer: 50 mM Tris-HCl, 10 mM MgCl2, 5 mM Dithiothreitol, 0.08 g/l bovine serum albumin
PH: 7.6
Temperature: 37
uniprot:P06855 [polynucleotide 5'-hydroxyl-kinase(Enzyme) wildtype]

ATP + 5'_Dephospho_DNA -> ADP + 5'_Phospho_DNA		 (( (Vmax * A) ) / (KmA + A)) buffer: 50 mM Tris-HCl, 10 mM MgCl2, 5 mM Dithiothreitol, 0.08 g/l bovine serum albumin
PH: 7.6
Temperature: 37
uniprot:P06855 [polynucleotide 5'-hydroxyl-kinase(Enzyme) mutant pseT 1,47]

5'_Dephospho_DNA + ATP -> ADP + 5'_Phospho_DNA		 (( (Vmax * A) ) / (KmA + A)) buffer: 50 mM Tris-HCl, 10 mM MgCl2, 5 mM Dithiothreitol, 0.08 g/l bovine serum albumin
PH: 7.6
Temperature: 37
uniprot:P06855 [polynucleotide 5'-hydroxyl-kinase(Enzyme) mutant pseT 1,47]

ATP + 5'_Dephospho_DNA -> 5'_Phospho_DNA + ADP		 (( (Vmax * A) ) / (KmA + A)) buffer: 50 mM Tris-HCl, 10 mM MgCl2, 5 mM Dithiothreitol, 0.08 g/l bovine serum albumin
PH: 7.6
Temperature: 37
uniprot:P06855 [polynucleotide 5'-hydroxyl-kinase(Enzyme) mutant pseT 1]

5'_Dephospho_DNA + ATP -> ADP + 5'_Phospho_DNA		 (( (Vmax * A) ) / (KmA + A)) buffer: 50 mM Tris-HCl, 10 mM MgCl2, 5 mM Dithiothreitol, 0.08 g/l bovine serum albumin
PH: 7.6
Temperature: 37

Pathways

pathway id name
Reactome:R-GGA-9770562 mRNA Polyadenylation
Reactome:R-BTA-9770562 mRNA Polyadenylation
Reactome:R-DRE-9770562 mRNA Polyadenylation
Reactome:R-MMU-9770562 mRNA Polyadenylation
Reactome:R-RNO-9770562 mRNA Polyadenylation
Reactome:R-XTR-9770562 mRNA Polyadenylation
Reactome:R-HSA-9770562 mRNA Polyadenylation
Reactome:R-DRE-73856 RNA Polymerase II Transcription Termination
Reactome:R-BTA-74160 Gene expression (Transcription)
Reactome:R-BTA-73857 RNA Polymerase II Transcription
Reactome:R-BTA-73856 RNA Polymerase II Transcription Termination
Reactome:R-DRE-75067 Processing of Capped Intronless Pre-mRNA
Reactome:R-DRE-72312 rRNA processing
Reactome:R-HSA-74160 Gene expression (Transcription)
Reactome:R-HSA-73857 RNA Polymerase II Transcription
Reactome:R-HSA-6791226 Major pathway of rRNA processing in the nucleolus and cytosol
Reactome:R-HSA-72306 tRNA processing
Reactome:R-MMU-73933 Resolution of Abasic Sites (AP sites)
Reactome:R-MMU-5649702 APEX1-Independent Resolution of AP Sites via the Single Nucleotide Replacement Pathway
Reactome:R-MMU-8953854 Metabolism of RNA
Reactome:R-MMU-77595 Processing of Intronless Pre-mRNAs
Reactome:R-MMU-6791226 Major pathway of rRNA processing in the nucleolus and cytosol
Reactome:R-MMU-74160 Gene expression (Transcription)
Reactome:R-MMU-73857 RNA Polymerase II Transcription
Reactome:R-MMU-73856 RNA Polymerase II Transcription Termination
Reactome:R-RNO-72203 Processing of Capped Intron-Containing Pre-mRNA
Reactome:R-RNO-72187 mRNA 3'-end processing
Reactome:R-XTR-72203 Processing of Capped Intron-Containing Pre-mRNA
Reactome:R-XTR-72187 mRNA 3'-end processing
Reactome:R-BTA-72203 Processing of Capped Intron-Containing Pre-mRNA
Reactome:R-BTA-72187 mRNA 3'-end processing
Reactome:R-DRE-8953854 Metabolism of RNA
Reactome:R-GGA-72203 Processing of Capped Intron-Containing Pre-mRNA
Reactome:R-GGA-72187 mRNA 3'-end processing
Reactome:R-HSA-73884 Base Excision Repair
Reactome:R-HSA-73933 Resolution of Abasic Sites (AP sites)
Reactome:R-HSA-5649702 APEX1-Independent Resolution of AP Sites via the Single Nucleotide Replacement Pathway
Reactome:R-HSA-73856 RNA Polymerase II Transcription Termination
Reactome:R-HSA-72203 Processing of Capped Intron-Containing Pre-mRNA
Reactome:R-HSA-72187 mRNA 3'-end processing
Reactome:R-HSA-8868773 rRNA processing in the nucleus and cytosol
Reactome:R-MMU-8868773 rRNA processing in the nucleus and cytosol
Reactome:R-RNO-75067 Processing of Capped Intronless Pre-mRNA
Reactome:R-BTA-8953854 Metabolism of RNA
Reactome:R-BTA-77595 Processing of Intronless Pre-mRNAs
Reactome:R-DRE-72203 Processing of Capped Intron-Containing Pre-mRNA
Reactome:R-DRE-72187 mRNA 3'-end processing
Reactome:R-DRE-77595 Processing of Intronless Pre-mRNAs
Reactome:R-DRE-8868773 rRNA processing in the nucleus and cytosol
Reactome:R-GGA-8953854 Metabolism of RNA
Reactome:R-GGA-74160 Gene expression (Transcription)
Reactome:R-GGA-73857 RNA Polymerase II Transcription
Reactome:R-GGA-73856 RNA Polymerase II Transcription Termination
Reactome:R-HSA-8953854 Metabolism of RNA
Reactome:R-HSA-77595 Processing of Intronless Pre-mRNAs
Reactome:R-HSA-6784531 tRNA processing in the nucleus
Reactome:R-MMU-73894 DNA Repair
Reactome:R-MMU-75067 Processing of Capped Intronless Pre-mRNA
Reactome:R-RNO-74160 Gene expression (Transcription)
Reactome:R-RNO-73857 RNA Polymerase II Transcription
Reactome:R-BTA-75067 Processing of Capped Intronless Pre-mRNA
Reactome:R-DRE-6791226 Major pathway of rRNA processing in the nucleolus and cytosol
Reactome:R-DRE-74160 Gene expression (Transcription)
Reactome:R-DRE-73857 RNA Polymerase II Transcription
Reactome:R-HSA-73894 DNA Repair
Reactome:R-HSA-75067 Processing of Capped Intronless Pre-mRNA
Reactome:R-HSA-72312 rRNA processing
Reactome:R-MMU-73884 Base Excision Repair
Reactome:R-MMU-72203 Processing of Capped Intron-Containing Pre-mRNA
Reactome:R-MMU-72187 mRNA 3'-end processing
Reactome:R-MMU-72312 rRNA processing
Reactome:R-RNO-8953854 Metabolism of RNA
Reactome:R-RNO-77595 Processing of Intronless Pre-mRNAs
Reactome:R-RNO-73856 RNA Polymerase II Transcription Termination
Reactome:R-XTR-8953854 Metabolism of RNA