uniprot:P17709 [glucokinase(Enzyme) wildtype isozyme Glk1p]
ATP + D_Glucose -> D_Glucose_6_phosphate + ADP |
(( (( (GLK1 * HXKGLK1kcat) ) * (( (( (( (0 - ADP) ) * G6P) ) / (( (HXKGLK1Katp * HXKGLK1Kglc) ) * KeqHXK)) ) + (( (ATP * GLC) ) / (HXKGLK1Katp * HXKGLK1Kglc)))) ) / (( (( (1 + (ADP / HXKGLK1Kadp)) ) + (ATP / HXKGLK1Katp)) ) * (( (1 + (G6P / HXKGLK1Kg6p)) ) + (GLC / HXKGLK1Kglc)))) |
|
buffer: *
PH: 7
Temperature: 36 |
- [D-Fructose]
D_Glucose + ATP -> ADP + D_Glucose_6_phosphate |
(( (( (( (( (( (vol * Vmax3) ) * Glc) ) / Km3Glc) ) * ATP) ) / Km3ATP) ) / (( (1 + (ATP / Km3ATP)) ) * (( (( (( (1 + (Glc / Km3Glc)) ) + (Fru / Km4Fru)) ) + (( (0.113 * HexP) ) / Ki3G6P)) ) + (( (0.0575 * HexP) ) / Ki4F6P)))) |
|
buffer: *
PH: 7
Temperature: 36 |
uniprot:P35557 [glucokinase(Enzyme) wildtype]
D_Glucose + ATP -> D_Glucose_6_phosphate + ADP |
(( (V * A) ) / (Km + A)) |
|
buffer: 50 mM Hepes-NaOH, 60 mM KCl, 10 mM KH2PO4, 1 mM L-cysteine, 1 mM EDTA, 6 mM MgCl2
PH: 7.5
Temperature: 30 |
uniprot:P35557 [alpha-D-Mannoheptulopyranose]
ATP + D_Glucose -> D_Glucose_6_phosphate + ADP |
(( (V * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) |
|
buffer: 50 mM Hepes-NaOH, 60 mM KCl, 10 mM KH2PO4, 1 mM L-cysteine, 1 mM EDTA, 6 mM MgCl2
PH: 7.5
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) wildtype]
ATP + D_Glucose -> D_Glucose_6_phosphate + ADP |
(( (V * A) ) / (( (Km * (1 + (I / Ki))) ) + (A * (1 + (I / Ki))))) |
|
buffer: 50 mM Hepes-NaOH, 60 mM KCl, 10 mM KH2PO4, 1 mM L-cysteine, 1 mM EDTA, 6 mM MgCl2
PH: 7.5
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) wildtype]
ATP + D_Glucose -> D_Glucose_6_phosphate + ADP |
(( (V * (A ^ n)) ) / (( (Km ^ n) ) + (A ^ n))) |
|
buffer: 50 mM Hepes-NaOH, 60 mM KCl, 10 mM KH2PO4, 1 mM L-cysteine, 1 mM EDTA, 6 mM MgCl2
PH: 7.5
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) wildtype]
ATP + D_Fructose -> D_Fructose_6_phosphate + ADP |
(( (V * A) ) / (Km + A)) |
|
buffer: 50 mM Hepes-NaOH, 60 mM KCl, 10 mM KH2PO4, 1 mM L-cysteine, 1 mM EDTA, 6 mM MgCl2
PH: 7.5
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) wildtype]
ATP + D_Mannose -> ADP + D_Mannose_6_phosphate |
(( (V * A) ) / (Km + A)) |
|
buffer: 50 mM Hepes-NaOH, 60 mM KCl, 10 mM KH2PO4, 1 mM L-cysteine, 1 mM EDTA, 6 mM MgCl2
PH: 7.5
Temperature: 30 |
uniprot:P17712 [glucokinase(Enzyme) wildtype fusion protein isoform B1-GST]
ATP + D_Glucose -> ADP + D_Glucose_6_phosphate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 50 mM HEPES-HCl, 100 mM KCl, 7.4 mM MgCl2, 15 mM 2-Mercaptoethanol, 0.05 % BSA, 0.5 mM NAD+, 0.70 U/ml Glucose-6-phosphate dehydrogenase
PH: 7.6
Temperature: 30 |
uniprot:P17712 [glucokinase(Enzyme) wildtype isoform B1]
D_Glucose + ATP -> D_Glucose_6_phosphate + ADP |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 50 mM HEPES-HCl, 100 mM KCl, 7.4 mM MgCl2, 15 mM 2-Mercaptoethanol, 0.05 % BSA, 0.5 mM NAD+, 0.70 U/ml Glucose-6-phosphate dehydrogenase
PH: 7.6
Temperature: 30 |
uniprot:P17712 [glucokinase(Enzyme) wildtype isoform L1]
D_Glucose + ATP -> ADP + D_Glucose_6_phosphate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 50 mM HEPES-HCl, 100 mM KCl, 7.4 mM MgCl2, 15 mM 2-Mercaptoethanol, 0.05 % BSA, 0.5 mM NAD+, 0.70 U/ml Glucose-6-phosphate dehydrogenase
PH: 7.6
Temperature: 30 |
uniprot:P17712 [glucokinase(Enzyme) mutant isoform B1 Met-delta8]
ATP + D_Glucose -> ADP + D_Glucose_6_phosphate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 50 mM HEPES-HCl, 100 mM KCl, 7.4 mM MgCl2, 15 mM 2-Mercaptoethanol, 0.05 % BSA, 0.5 mM NAD+, 0.70 U/ml Glucose-6-phosphate dehydrogenase
PH: 7.6
Temperature: 30 |
- [glucokinase(Enzyme) wildtype]
ATP + D_Glucose -> ADP + D_Glucose_6_phosphate |
(( (V * A) ) / (Km + A)) |
|
buffer: 0.03 M Tris, 0.006 M MgCl2, 0.0006 M EDTA, 0.0002 M NADP, 1.40 K units/ml glucose-6-phosphate dehydrogenase
PH: 8
Temperature: 30 |
- [glucokinase(Enzyme) wildtype]
Glucose + ATP -> Glucose_6_phosphate + ADP |
(( (V * S) ) / (( (Km * (1 + (I / Kis))) ) + (S * (1 + (I / Kii))))) |
|
buffer: 0.2 M HEPES, 1 mM NADP, 10 mM Mg2+, 10 U/ml glucose 6-phosphate dehydrogenase
PH: 7.5
Temperature: 22 |
- [Mg2+]
ATP + Glucose -> ADP + Glucose_6_phosphate |
(( (V * S) ) / (Km + S)) |
|
buffer: 0.2 M HEPES, 1 mM NADP, 10 mM Mg2+, 10 U/ml glucose 6-phosphate dehydrogenase
PH: 7.5
Temperature: 36 |
- [glucokinase(Enzyme) wildtype]
Glucose + ATP -> Glucose_6_phosphate + ADP |
(( (V * S) ) / (Km + (S * (1 + (I / Kii))))) |
|
buffer: 0.2 M HEPES, 1 mM NADP, 10 mM Mg2+, 10 U/ml glucose 6-phosphate dehydrogenase
PH: 7.5
Temperature: 22 |
- [glucokinase(Enzyme) wildtype]
ATP + Glucose -> Glucose_6_phosphate + ADP |
(( (( (V * A) ) * B) ) / (( (( (Kia + (KmA * B)) ) + (KmB * A)) ) + (A * B))) |
|
buffer: 0.2 M HEPES, 1 mM NADP, 10 mM Mg2+, 10 U/ml glucose 6-phosphate dehydrogenase
PH: 7.5
Temperature: 22 |
- [glucokinase(Enzyme) wildtype]
D_Glucose + ATP -> D_Glucose_1_phosphate + ADP |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 50 mM Bicine-NaOH, 5 mM MgCl2, 1 mM NAD, 1 IU/ml glucose 6-P dehydrogenase
PH: 8.5
Temperature: 30 |
- [glucokinase(Enzyme) wildtype]
ATP + D_Glucose -> D_Glucose_1_phosphate + ADP |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 50 mM Bicine-NaOH, 5 mM MgCl2, 1 mM NAD, 1 IU/ml glucose 6-P dehydrogenase
PH: 8.5
Temperature: 30 |
- [glucokinase(Enzyme) wildtype]
ATP + D_Glucose -> D_Glucose_1_phosphate + ADP |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 50 mM Bicine-NaOH, 5 mM MgCl2, 1 mM NAD, 1 IU/ml glucose 6-P dehydrogenase
PH: 8.5
Temperature: 30 |
- [glucokinase(Enzyme) wildtype]
D_Glucose + ATP -> D_Glucose_1_phosphate + ADP |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 50 mM Bicine-NaOH, 5 mM MgCl2, 1 mM NAD, 1 IU/ml glucose 6-P dehydrogenase
PH: 8.5
Temperature: 30 |
uniprot:P17712 [glucokinase(Enzyme) wildtype]
D_Glucose + ATP -> ADP + D_Glucose_6_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM glycylglycine, 7.5 mM MgSO4, 0.5 mM NADP+ 100 mM KCl, 0.27 units/ml glucose 6-phosphate dehydrogenase
PH: 7.5
Temperature: 28 |
uniprot:P17712 [glucokinase(Enzyme) wildtype]
2_Deoxy_D_glucose + ATP -> ADP + 2_Deoxy_D_glucose_6_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM triethanolamine hydrochloride, 7.5 mM MgSO4, 100 mM KCl, 0.75 mM phosphoenolpyruvate, 0.25 mM NADH, 12.5 µg/ml pyruvate kinase, 25 µg/ml lactate dehydrogenase
PH: 7.5
Temperature: 28 |
uniprot:P17712 [glucokinase(Enzyme) wildtype]
D_Glucose + ATP -> D_Glucose_6_phosphate + ADP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM triethanolamine hydrochloride, 7.5 mM MgSO4, 100 mM KCl, 0.75 mM phosphoenolpyruvate, 0.25 mM NADH, 12.5 µg/ml pyruvate kinase, 25 µg/ml lactate dehydrogenase
PH: 7.5
Temperature: 28 |
- [glucokinase(Enzyme) wildtype]
2_Deoxy_D_glucose + Phosphate -> H2O + 2_Deoxy_D_glucose_6_phosphate |
(( (Vmax * S) ) / (S + Km)) |
|
buffer: Imidazole-HCl, 1.0 mM EDTA
PH: 6.8
Temperature: 37 |
- [glucokinase(Enzyme) wildtype]
2_Deoxy_D_glucose + Phosphate -> 2_Deoxy_D_glucose_6_phosphate + H2O |
(( (Vmax * S) ) / (S + Km)) |
|
buffer: Imidazole-HCl, 1.0 mM EDTA
PH: 6.8
Temperature: 37 |
uniprot:P17712 [glucokinase(Enzyme) wildtype]
D_Glucose + ATP -> ADP + D_Glucose_6_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM glycylglycine, 7.5 mM MgSO4, 0.5 mM NADP+ 100 mM KCl, 0.27 units/ml glucose 6-phosphate dehydrogenase
PH: 7.5
Temperature: 28 |
uniprot:P17712 [glucokinase(Enzyme) wildtype]
D_Mannose + ATP -> ADP + D_Mannose_6_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM triethanolamine hydrochloride, 7.5 mM MgSO4, 100 mM KCl, 0.75 mM phosphoenolpyruvate, 0.25 mM NADH, 12.5 µg/ml pyruvate kinase, 25 µg/ml lactate dehydrogenase
PH: 7.5
Temperature: 28 |
uniprot:A5U654 [glucokinase(Enzyme) wildtype]
D_Glucose + ATP -> ADP + D_Glucose_6_phosphate |
(( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (( (Kia * Kb) ) + (Ka * B)) ) + (( (Kb * A) ) * (1 + (A / Ksi)))) ) + (A * B))) |
|
buffer: 100 mM Tris-HCl, 200 mM NaCl, 6 mM MgCl2, 0.6 mM NAD, 4 U/ml Glucose-6-phosphate dehydrogenase
PH: 7.5
Temperature: 30 |
uniprot:A5U654 [glucokinase(Enzyme) wildtype]
ATP + D_Glucose -> D_Glucose_6_phosphate + ADP |
(( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (( (Kia * Kb) ) + (Ka * B)) ) + (Kb * A)) ) + (A * B))) |
|
buffer: 100 mM Tris-HCl, 200 mM NaCl, 6 mM MgCl2, 0.6 mM NAD, 4 U/ml Glucose-6-phosphate dehydrogenase
PH: 8.6
Temperature: 30 |
uniprot:A5U654 [glucokinase(Enzyme) wildtype]
D_Glucose + ATP -> ADP + D_Glucose_6_phosphate |
(( (V * A) ) / (( (K * (1 + (I / Kis))) ) + (A * (1 + (I / Kii))))) |
|
buffer: 100 mM Tris-HCl, 200 mM NaCl, 6 mM MgCl2, 0.58 mM NAD, 4 U/ml Glucose-6-phosphate dehydrogenase
PH: 7.5
Temperature: 30 |
uniprot:A5U654 [glucokinase(Enzyme) wildtype]
ATP + D_Glucose -> D_Glucose_6_phosphate + ADP |
(( (V * A) ) / (( (K * (1 + (I / Kis))) ) + (A * (1 + (I / Kii))))) |
|
buffer: 100 mM Tris-HCl, 200 mM NaCl, 6 mM MgCl2, 0.58 mM NAD, 4 U/ml Glucose-6-phosphate dehydrogenase
PH: 7.5
Temperature: 30 |
uniprot:A5U654 [AMP]
D_Glucose + ATP -> ADP + D_Glucose_6_phosphate |
(( (V * A) ) / (( (K * (1 + (I / Kis))) ) + (A * (1 + (I / Kii))))) |
|
buffer: 100 mM Tris-HCl, 200 mM NaCl, 6 mM MgCl2, 0.58 mM NAD, 4 U/ml Glucose-6-phosphate dehydrogenase
PH: 7.5
Temperature: 30 |
uniprot:A5U654 [AMP]
D_Glucose + ATP -> D_Glucose_6_phosphate + ADP |
(( (V * A) ) / (( (K * (1 + (I / Kis))) ) + A)) |
|
buffer: 100 mM Tris-HCl, 200 mM NaCl, 6 mM MgCl2, 0.58 mM NAD, 4 U/ml Glucose-6-phosphate dehydrogenase
PH: 7.5
Temperature: 30 |
uniprot:A5U654 [Fructose 6-phosphate]
D_Glucose + ATP -> ADP + D_Glucose_6_phosphate |
(( (V * A) ) / (( (K * (1 + (I / Kis))) ) + (A * (1 + (I / Kii))))) |
|
buffer: 100 mM Tris-HCl, 200 mM NaCl, 6 mM MgCl2, 0.58 mM NAD, 4 U/ml Glucose-6-phosphate dehydrogenase
PH: 7.5
Temperature: 30 |
uniprot:A5U654 [Fructose 6-phosphate]
D_Glucose + ATP -> D_Glucose_6_phosphate + ADP |
(( (V * A) ) / (( (K * (1 + (I / Kis))) ) + A)) |
|
buffer: 100 mM Tris-HCl, 200 mM NaCl, 6 mM MgCl2, 0.58 mM NAD, 4 U/ml Glucose-6-phosphate dehydrogenase
PH: 7.5
Temperature: 30 |
uniprot:A5U654 [Fructose 6-phosphate]
ATP + D_Glucose -> D_Glucose_6_phosphate + ADP |
(( (V * A) ) / (( (K * (1 + (I / Kis))) ) + A)) |
|
buffer: 100 mM Tris-HCl, 200 mM NaCl, 6 mM MgCl2, 0.58 mM NAD, 4 U/ml Glucose-6-phosphate dehydrogenase
PH: 7.5
Temperature: 30 |
uniprot:A5U654 [glucokinase(Enzyme) wildtype]
ATP + D_Glucose -> ADP + D_Glucose_6_phosphate |
(( (V * A) ) / (K + (A * (1 + (I / Kii))))) |
|
buffer: 100 mM Tris-HCl, 200 mM NaCl, 6 mM MgCl2, 0.58 mM NAD, 4 U/ml Glucose-6-phosphate dehydrogenase
PH: 7.5
Temperature: 30 |
uniprot:A5U654 [glucokinase(Enzyme) wildtype]
ATP + D_Glucose -> D_Glucose_6_phosphate + ADP |
(( (V * A) ) / (( (K * (1 + (I / Kis))) ) + A)) |
|
buffer: 100 mM Tris-HCl, 200 mM NaCl, 6 mM MgCl2, 0.58 mM NAD, 4 U/ml Glucose-6-phosphate dehydrogenase
PH: 7.5
Temperature: 30 |
uniprot:P17709 [glucokinase(Enzyme) wildtype isozyme Glk1p, C-terminal GST-tag]
ATP + D_Glucose -> ADP + D_Glucose_6_phosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 100 mM MES, 100 mM KCl, 5 mM NADP, 50 U/ml glucose-6-P-dehydrogenase, 5 mM Mg2+
PH: 6.5
Temperature: 30 |
uniprot:P17709 [glucokinase(Enzyme) wildtype isozyme Glk1p, C-terminal GST-tag]
ATP + D_Glucose -> D_Glucose_6_phosphate + ADP |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 100 mM MES, 100 mM KCl, 5 mM NADP, 50 U/ml glucose-6-P- dehydrogenase, 5 mM Mg2+
PH: 6.5
Temperature: 30 |
- [glucokinase(Enzyme) wildtype]
ATP + alpha_D_Glucose -> alpha_D_Glucose_6_phosphate + ADP |
(( (( (( (cytosol * VmGLK) ) / (KmGLKGLCi * KmGLKATP)) ) * (( (GLCi * ATP) ) - (( (G6P * ADP) ) / KeqGLK))) ) / (( (( (1 + (GLCi / KmGLKGLCi)) ) + (G6P / KmGLKG6P)) ) * (( (1 + (ATP / KmGLKATP)) ) + (ADP / KmGLKADP)))) |
|
buffer: *
PH: 7
Temperature: 36 |
- [glucokinase(Enzyme) wildtype]
D_Glucose + ATP -> D_Glucose_6_phosphate + ADP |
(( (Vmax * (A ^ n)) ) / (( (S_half ^ n) ) + (A ^ n))) |
|
buffer: 80 mM Tris, 5 mM EDTA, 8 mM MgSO4, 1 mM KH2PO4, 2 mM NaHCO3, 2.5 mM dithioerythriol, 2 mM NADP+, 65 mU/ml glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase
PH: 8
Temperature: 30 |
uniprot:P17712 [glucokinase(Enzyme) wildtype]
ATP + alpha_D_Glucose -> ADP + alpha_D_Glucose_6_phosphate |
(( (Vmax * (A ^ h)) ) / (( (S05 ^ h) ) + (A ^ h))) |
|
buffer: 50 mM Hepes, 100 mM KCL, 1 mM dithiothreitol, 0.5% bovine serum albumin, 1-3 mM MgCl2
PH: 7.6
Temperature: 25 |
uniprot:P17712 [glucokinase(Enzyme) wildtype]
ATP + beta_D_Glucose -> beta_D_Glucose_6_phosphate + ADP |
(( (Vmax * (A ^ h)) ) / (( (S05 ^ h) ) + (A ^ h))) |
|
buffer: 50 mM Hepes, 100 mM KCL, 1 mM dithiothreitol, 0.5% bovine serum albumin, 1-3 mM MgCl2
PH: 7.6
Temperature: 25 |
uniprot:P17712 [glucokinase(Enzyme) wildtype]
beta_D_Glucose + ATP -> ADP + beta_D_Glucose_6_phosphate |
(( (Vmax * (A ^ h)) ) / (( (S05 ^ h) ) + (A ^ h))) |
|
buffer: 50 mM Hepes, 100 mM KCL, 1 mM dithiothreitol, 0.5% bovine serum albumin, 1-3 mM MgCl2
PH: 7.6
Temperature: 25 |
uniprot:P17712 [glucokinase(Enzyme) wildtype]
ATP + alpha_D_Glucose -> ADP + alpha_D_Glucose_6_phosphate |
(( (Vmax * (A ^ h)) ) / (( (S05 ^ h) ) + (A ^ h))) |
|
buffer: 50 mM Hepes, 100 mM KCL, 1 mM dithiothreitol, 0.5% bovine serum albumin, 1-3 mM MgCl2
PH: 7.6
Temperature: 25 |
uniprot:P35557 [glucokinase(Enzyme) wildtype]
D_Glucose + ATP -> ADP + D_Glucose_6_phosphate |
(( (( (E * kcat) ) * (S ^ h)) ) / (K + (S ^ h))) |
|
buffer: -
PH: 7
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant Asn204Ser]
D_Glucose + ATP -> D_Glucose_6_phosphate + ADP |
(( (( (E * kcat) ) * (S ^ h)) ) / (K + (S ^ h))) |
|
buffer: -
PH: 7
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant Asn204Gln]
ATP + D_Glucose -> ADP + D_Glucose_6_phosphate |
(( (( (E * kcat) ) * (S ^ h)) ) / (K + (S ^ h))) |
|
buffer: -
PH: 7
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant Asn204Ala]
D_Glucose + ATP -> ADP + D_Glucose_6_phosphate |
(( (( (E * kcat) ) * (S ^ h)) ) / (K + (S ^ h))) |
|
buffer: -
PH: 7
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant Asn204Asp]
D_Glucose + ATP -> ADP + D_Glucose_6_phosphate |
(( (( (E * kcat) ) * (S ^ h)) ) / (K + (S ^ h))) |
|
buffer: -
PH: 7
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) wildtype]
D_Mannose + ATP -> ADP + D_Mannose_6_phosphate |
(( (( (E * kcat) ) * (S ^ h)) ) / (K + (S ^ h))) |
|
buffer: 100 mM Tris HCl, 100 mM KCl, 20 mM MgCl2, 0.6 mM phosphoenolpyruvate, 0.6 mM NADH, 1.76 units/ml lactate dehydrogenase, 1.4 units/ml pyruvate kinase
PH: 7.5
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant Asn204Asp]
2_Deoxyglucose + ATP -> 2_Deoxyglucose_6_phosphate + ADP |
(( (( (E * kcat) ) * (S ^ h)) ) / (K + (S ^ h))) |
|
buffer: 100 mM Tris HCl, 100 mM KCl, 20 mM MgCl2, 0.6 mM phosphoenolpyruvate, 0.6 mM NADH, 1.76 units/ml lactate dehydrogenase, 1.4 units/ml pyruvate kinase
PH: 7.5
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) wildtype]
ATP + 2_Deoxyglucose -> ADP + 2_Deoxyglucose_6_phosphate |
(( (( (E * kcat) ) * (S ^ h)) ) / (K + (S ^ h))) |
|
buffer: 100 mM Tris HCl, 100 mM KCl, 20 mM MgCl2, 0.6 mM phosphoenolpyruvate, 0.6 mM NADH, 1.76 units/ml lactate dehydrogenase, 1.4 units/ml pyruvate kinase
PH: 7.5
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant Asn204Gln]
2_Deoxyglucose + ATP -> 2_Deoxyglucose_6_phosphate + ADP |
(( (( (E * kcat) ) * (S ^ h)) ) / (K + (S ^ h))) |
|
buffer: 100 mM Tris HCl, 100 mM KCl, 20 mM MgCl2, 0.6 mM phosphoenolpyruvate, 0.6 mM NADH, 1.76 units/ml lactate dehydrogenase, 1.4 units/ml pyruvate kinase
PH: 7.5
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant Asn204Ser]
ATP + 2_Deoxyglucose -> 2_Deoxyglucose_6_phosphate + ADP |
(( (( (E * kcat) ) * (S ^ h)) ) / (K + (S ^ h))) |
|
buffer: 100 mM Tris HCl, 100 mM KCl, 20 mM MgCl2, 0.6 mM phosphoenolpyruvate, 0.6 mM NADH, 1.76 units/ml lactate dehydrogenase, 1.4 units/ml pyruvate kinase
PH: 7.5
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant Asn204Ala]
2_Deoxyglucose + ATP -> 2_Deoxyglucose_6_phosphate + ADP |
(( (( (E * kcat) ) * (S ^ h)) ) / (K + (S ^ h))) |
|
buffer: 100 mM Tris HCl, 100 mM KCl, 20 mM MgCl2, 0.6 mM phosphoenolpyruvate, 0.6 mM NADH, 1.76 units/ml lactate dehydrogenase, 1.4 units/ml pyruvate kinase
PH: 7.5
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) wildtype]
ATP + D_Fructose -> ADP + D_Fructose_6_phosphate |
(( (( (E * kcat) ) * (S ^ h)) ) / (K + (S ^ h))) |
|
buffer: 100 mM Tris HCl, 100 mM KCl, 20 mM MgCl2, 0.6 mM phosphoenolpyruvate, 0.6 mM NADH, 1.76 units/ml lactate dehydrogenase, 1.4 units/ml pyruvate kinase
PH: 7.5
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant Asn204Ser]
D_Fructose + ATP -> ADP + D_Fructose_6_phosphate |
(( (( (E * kcat) ) * (S ^ h)) ) / (K + (S ^ h))) |
|
buffer: 100 mM Tris HCl, 100 mM KCl, 20 mM MgCl2, 0.6 mM phosphoenolpyruvate, 0.6 mM NADH, 1.76 units/ml lactate dehydrogenase, 1.4 units/ml pyruvate kinase
PH: 7.5
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) wildtype]
ATP + D_Glucosamine -> ADP + D_Glucosamine_6_phosphate |
(( (( (E * kcat) ) * S) ) / (( (Km + S) ) + (( (S ^ 2) ) / Ki))) |
|
buffer: 100 mM Tris HCl, 100 mM KCl, 20 mM MgCl2, 0.6 mM phosphoenolpyruvate, 0.6 mM NADH, 1.76 units/ml lactate dehydrogenase, 1.4 units/ml pyruvate kinase
PH: 9
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant Asn204Ser]
D_Glucosamine + ATP -> D_Glucosamine_6_phosphate + ADP |
(( (( (E * kcat) ) * S) ) / (( (Km + S) ) + (( (S ^ 2) ) / Ki))) |
|
buffer: 100 mM Tris HCl, 100 mM KCl, 20 mM MgCl2, 0.6 mM phosphoenolpyruvate, 0.6 mM NADH, 1.76 units/ml lactate dehydrogenase, 1.4 units/ml pyruvate kinase
PH: 9
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant Asn204Gln]
ATP + D_Glucosamine -> D_Glucosamine_6_phosphate + ADP |
(( (( (E * kcat) ) * S) ) / (( (Km + S) ) + (( (S ^ 2) ) / Ki))) |
|
buffer: 100 mM Tris HCl, 100 mM KCl, 20 mM MgCl2, 0.6 mM phosphoenolpyruvate, 0.6 mM NADH, 1.76 units/ml lactate dehydrogenase, 1.4 units/ml pyruvate kinase
PH: 9
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant Asn204Ala]
D_Glucosamine + ATP -> D_Glucosamine_6_phosphate + ADP |
(( (( (E * kcat) ) * S) ) / (( (Km + S) ) + (( (S ^ 2) ) / Ki))) |
|
buffer: 100 mM Tris HCl, 100 mM KCl, 20 mM MgCl2, 0.6 mM phosphoenolpyruvate, 0.6 mM NADH, 1.76 units/ml lactate dehydrogenase, 1.4 units/ml pyruvate kinase
PH: 9
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant Asn204Ser]
ATP + D_Mannose -> ADP + D_Mannose_6_phosphate |
(( (( (E * kcat) ) * (S ^ h)) ) / (K + (S ^ h))) |
|
buffer: 100 mM Tris HCl, 100 mM KCl, 20 mM MgCl2, 0.6 mM phosphoenolpyruvate, 0.6 mM NADH, 1.76 units/ml lactate dehydrogenase, 1.4 units/ml pyruvate kinase
PH: 7.5
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant Asn204Gln]
D_Mannose + ATP -> D_Mannose_6_phosphate + ADP |
(( (( (E * kcat) ) * (S ^ h)) ) / (K + (S ^ h))) |
|
buffer: 100 mM Tris HCl, 100 mM KCl, 20 mM MgCl2, 0.6 mM phosphoenolpyruvate, 0.6 mM NADH, 1.76 units/ml lactate dehydrogenase, 1.4 units/ml pyruvate kinase
PH: 7.5
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant Asn204Ala]
D_Mannose + ATP -> D_Mannose_6_phosphate + ADP |
(( (( (E * kcat) ) * (S ^ h)) ) / (K + (S ^ h))) |
|
buffer: 100 mM Tris HCl, 100 mM KCl, 20 mM MgCl2, 0.6 mM phosphoenolpyruvate, 0.6 mM NADH, 1.76 units/ml lactate dehydrogenase, 1.4 units/ml pyruvate kinase
PH: 7.5
Temperature: 30 |
uniprot:P35557 [N-Acetylglucosamine]
D_Glucose + ATP -> ADP + D_Glucose_6_phosphate |
(( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) |
|
buffer: -
PH: 7
Temperature: 30 |
uniprot:P35557 [N-Acetylglucosamine]
ATP + D_Glucose -> ADP + D_Glucose_6_phosphate |
(( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) |
|
buffer: -
PH: 7
Temperature: 30 |
uniprot:P35557 [N-Acetylglucosamine]
ATP + D_Glucose -> ADP + D_Glucose_6_phosphate |
(( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) |
|
buffer: -
PH: 7
Temperature: 30 |
uniprot:P35557 [N-Acetylglucosamine]
D_Glucose + ATP -> D_Glucose_6_phosphate + ADP |
(( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) |
|
buffer: -
PH: 7
Temperature: 30 |
uniprot:P35557 [alpha-D-Mannoheptulopyranose]
ATP + D_Glucose -> ADP + D_Glucose_6_phosphate |
(( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) |
|
buffer: -
PH: 7
Temperature: 30 |
uniprot:P35557 [alpha-D-Mannoheptulopyranose]
D_Glucose + ATP -> D_Glucose_6_phosphate + ADP |
(( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) |
|
buffer: -
PH: 7
Temperature: 30 |
uniprot:P35557 [alpha-D-Mannoheptulopyranose]
ATP + D_Glucose -> ADP + D_Glucose_6_phosphate |
(( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) |
|
buffer: -
PH: 7
Temperature: 30 |
uniprot:P35557 [alpha-D-Mannoheptulopyranose]
ATP + D_Glucose -> D_Glucose_6_phosphate + ADP |
(( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) |
|
buffer: -
PH: 7
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant Asn204Asp]
D_Glucosamine + ATP -> D_Glucosamine_6_phosphate + ADP |
(( (( (E * kcat) ) * S) ) / (( (Km + S) ) + (( (S ^ 2) ) / Ki))) |
|
buffer: 100 mM Tris HCl, 100 mM KCl, 20 mM MgCl2, 0.6 mM phosphoenolpyruvate, 0.6 mM NADH, 1.76 units/ml lactate dehydrogenase, 1.4 units/ml pyruvate kinase
PH: 9
Temperature: 30 |
uniprot:P35557 [N-Acetylglucosamine]
D_Glucose + ATP -> D_Glucose_6_phosphate + ADP |
(( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) |
|
buffer: -
PH: 7
Temperature: 30 |
uniprot:P35557 [N-Acetylglucosamine]
D_Glucose + ATP -> ADP + D_Glucose_6_phosphate |
(( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) |
|
buffer: -
PH: 7
Temperature: 30 |
uniprot:P35557 [N-Acetylglucosamine]
ATP + D_Glucose -> ADP + D_Glucose_6_phosphate |
(( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) |
|
buffer: -
PH: 7
Temperature: 30 |
uniprot:P35557 [alpha-D-Mannoheptulopyranose]
ATP + D_Glucose -> D_Glucose_6_phosphate + ADP |
(( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) |
|
buffer: -
PH: 7
Temperature: 30 |
uniprot:P35557 [alpha-D-Mannoheptulopyranose]
D_Glucose + ATP -> ADP + D_Glucose_6_phosphate |
(( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) |
|
buffer: -
PH: 7
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) wildtype]
ATP + D_Glucose -> ADP + D_Glucose_6_phosphate |
(( (( (E * kcat) ) * (S ^ h)) ) / (K + (S ^ h))) |
|
buffer: -
PH: 7
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant Asn204Ser]
D_Glucose + ATP -> D_Glucose_6_phosphate + ADP |
(( (( (E * kcat) ) * (S ^ h)) ) / (K + (S ^ h))) |
|
buffer: -
PH: 7
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant Asn204Gln]
D_Glucose + ATP -> ADP + D_Glucose_6_phosphate |
(( (( (E * kcat) ) * (S ^ h)) ) / (K + (S ^ h))) |
|
buffer: -
PH: 7
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant Asn204Ala]
D_Glucose + ATP -> ADP + D_Glucose_6_phosphate |
(( (( (E * kcat) ) * (S ^ h)) ) / (K + (S ^ h))) |
|
buffer: -
PH: 7
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant Glu256Ala]
ATP + D_Glucose -> ADP + D_Glucose_6_phosphate |
(( (( (E * kcat) ) * (S ^ h)) ) / (K + (S ^ h))) |
|
buffer: -
PH: 7
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant Lys56Ala]
D_Glucose + ATP -> D_Glucose_6_phosphate + ADP |
(( (( (E * kcat) ) * (S ^ h)) ) / (K + (S ^ h))) |
|
buffer: -
PH: 7
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant Lys56Ala]
D_Glucose + ATP -> D_Glucose_6_phosphate + ADP |
(( (( (E * kcat) ) * (S ^ h)) ) / (K + (S ^ h))) |
|
buffer: -
PH: 7
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant Asn204Asp]
ATP + D_Glucose -> ADP + D_Glucose_6_phosphate |
(( (( (E * kcat) ) * (S ^ h)) ) / (K + (S ^ h))) |
|
buffer: -
PH: 7
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant Glu256Ala]
ATP + D_Glucose -> ADP + D_Glucose_6_phosphate |
(( (( (E * kcat) ) * (S ^ h)) ) / (K + (S ^ h))) |
|
buffer: -
PH: 7
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant Lys56Ala]
D_Fructose + ATP -> ADP + D_Fructose_6_phosphate |
(( (( (E * kcat) ) * (S ^ h)) ) / (K + (S ^ h))) |
|
buffer: 100 mM Tris HCl, 100 mM KCl, 20 mM MgCl2, 0.6 mM phosphoenolpyruvate, 0.6 mM NADH, 1.76 units/ml lactate dehydrogenase, 1.4 units/ml pyruvate kinase
PH: 7.5
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant Lys56Ala]
ATP + D_Mannose -> D_Mannose_6_phosphate + ADP |
(( (( (E * kcat) ) * (S ^ h)) ) / (K + (S ^ h))) |
|
buffer: 100 mM Tris HCl, 100 mM KCl, 20 mM MgCl2, 0.6 mM phosphoenolpyruvate, 0.6 mM NADH, 1.76 units/ml lactate dehydrogenase, 1.4 units/ml pyruvate kinase
PH: 7.5
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant Lys56Ala]
ATP + 2_Deoxyglucose -> 2_Deoxyglucose_6_phosphate + ADP |
(( (( (E * kcat) ) * (S ^ h)) ) / (K + (S ^ h))) |
|
buffer: 100 mM Tris HCl, 100 mM KCl, 20 mM MgCl2, 0.6 mM phosphoenolpyruvate, 0.6 mM NADH, 1.76 units/ml lactate dehydrogenase, 1.4 units/ml pyruvate kinase
PH: 7.5
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant Glu256Ala]
2_Deoxyglucose + ATP -> ADP + 2_Deoxyglucose_6_phosphate |
(( (( (E * kcat) ) * (S ^ h)) ) / (K + (S ^ h))) |
|
buffer: 100 mM Tris HCl, 100 mM KCl, 20 mM MgCl2, 0.6 mM phosphoenolpyruvate, 0.6 mM NADH, 1.76 units/ml lactate dehydrogenase, 1.4 units/ml pyruvate kinase
PH: 7.5
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant Glu290Ala]
ATP + D_Glucose -> ADP + D_Glucose_6_phosphate |
(( (( (E * kcat) ) * (S ^ h)) ) / (K + (S ^ h))) |
|
buffer: -
PH: 7
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant Glu290Ala]
D_Glucose + ATP -> D_Glucose_6_phosphate + ADP |
(( (( (E * kcat) ) * (S ^ h)) ) / (K + (S ^ h))) |
|
buffer: -
PH: 7
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant Glu290Ala]
ATP + D_Fructose -> D_Fructose_6_phosphate + ADP |
(( (( (E * kcat) ) * (S ^ h)) ) / (K + (S ^ h))) |
|
buffer: 100 mM Tris HCl, 100 mM KCl, 20 mM MgCl2, 0.6 mM phosphoenolpyruvate, 0.6 mM NADH, 1.76 units/ml lactate dehydrogenase, 1.4 units/ml pyruvate kinase
PH: 7.5
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant Glu290Ala]
ATP + D_Mannose -> D_Mannose_6_phosphate + ADP |
(( (( (E * kcat) ) * (S ^ h)) ) / (K + (S ^ h))) |
|
buffer: 100 mM Tris HCl, 100 mM KCl, 20 mM MgCl2, 0.6 mM phosphoenolpyruvate, 0.6 mM NADH, 1.76 units/ml lactate dehydrogenase, 1.4 units/ml pyruvate kinase
PH: 7.5
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant Glu290Ala]
ATP + 2_Deoxyglucose -> 2_Deoxyglucose_6_phosphate + ADP |
(( (( (E * kcat) ) * (S ^ h)) ) / (K + (S ^ h))) |
|
buffer: 100 mM Tris HCl, 100 mM KCl, 20 mM MgCl2, 0.6 mM phosphoenolpyruvate, 0.6 mM NADH, 1.76 units/ml lactate dehydrogenase, 1.4 units/ml pyruvate kinase
PH: 7.5
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) wildtype]
D_Glucose + ATP -> ADP + D_Glucose_6_phosphate |
(( (Vmax * (S ^ n)) ) / (K + (S ^ n))) |
|
buffer: 100 mM Tris-HCl, 100 mM KCl, 20 mM MgCl2, 0.6 mM Phosphoenolpyruvate, 0.2 mM NADH, 1.76 U/ml lactate dehydrogenase, 1.4 U/ml pyruvate kinase
PH: 7.5
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) wildtype]
D_Glucosamine + ATP -> D_Glucosamine_6_phosphate + ADP |
(( (Vmax * S) ) / (( (Km + S) ) + (( (S ^ 2) ) / Ki))) |
|
buffer: 100 mM Tris-HCl, 100 mM KCl, 20 mM MgCl2, 0.6 mM Phosphoenolpyruvate, 0.2 mM NADH, 1.76 U/ml lactate dehydrogenase, 1.4 U/ml pyruvate kinase
PH: 9
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) wildtype]
D_Fructose + ATP -> ADP + D_Fructose_6_phosphate |
(( (Vmax * (S ^ n)) ) / (K + (S ^ n))) |
|
buffer: 100 mM Tris-HCl, 100 mM KCl, 20 mM MgCl2, 0.6 mM Phosphoenolpyruvate, 0.2 mM NADH, 1.76 U/ml lactate dehydrogenase, 1.4 U/ml pyruvate kinase
PH: 7.5
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) wildtype]
D_Mannose + ATP -> D_Mannose_6_phosphate + ADP |
(( (Vmax * (S ^ n)) ) / (K + (S ^ n))) |
|
buffer: 100 mM Tris-HCl, 100 mM KCl, 20 mM MgCl2, 0.6 mM Phosphoenolpyruvate, 0.2 mM NADH, 1.76 U/ml lactate dehydrogenase, 1.4 U/ml pyruvate kinase
PH: 7.5
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) wildtype]
2_Deoxy_D_glucose + ATP -> 2_Deoxy_D_glucose_6_phosphate + ADP |
(( (Vmax * (S ^ n)) ) / (K + (S ^ n))) |
|
buffer: 100 mM Tris-HCl, 100 mM KCl, 20 mM MgCl2, 0.6 mM Phosphoenolpyruvate, 0.2 mM NADH, 1.76 U/ml lactate dehydrogenase, 1.4 U/ml pyruvate kinase
PH: 7.5
Temperature: 30 |
uniprot:P35557 [N-Acetyl-D-glucosamine]
D_Glucose + ATP -> ADP + D_Glucose_6_phosphate |
(( (Vmax * (S ^ n)) ) / (( (Km * (1 + (I / Ki))) ) + (S ^ n))) |
|
buffer: 100 mM Tris-HCl, 100 mM KCl, 20 mM MgCl2, 0.6 mM Phosphoenolpyruvate, 0.2 mM NADH, 1.76 U/ml lactate dehydrogenase, 1.4 U/ml pyruvate kinase
PH: 7.5
Temperature: 30 |
uniprot:P35557 [alpha-D-Mannoheptulopyranose]
D_Glucose + ATP -> D_Glucose_6_phosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (Km * (1 + (I / Ki))) ) + (S ^ n))) |
|
buffer: 100 mM Tris-HCl, 100 mM KCl, 20 mM MgCl2, 0.6 mM Phosphoenolpyruvate, 0.2 mM NADH, 1.76 U/ml lactate dehydrogenase, 1.4 U/ml pyruvate kinase
PH: 7.5
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) wildtype]
H2O + ATP -> ADP + Phosphate |
(( (Vmax * S) ) / (( (Km + S) ) + (( (S ^ 2) ) / Ki))) |
|
buffer: 50 mM Pipes, 5 mM MgCl2
PH: 7
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) wildtype]
D_Glucose + ATP -> D_Glucose_6_phosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_h ^ n) ) + (S ^ n))) |
|
buffer: 25 mM Hepes, 25 mM KCl, 5 mM mercaptoethanol, 1 mM NADP, 2.5 mM MgCl2, 2 units/ml glucose 6-phosphate dehydrogenase
PH: 7.1
Temperature: 22 |
uniprot:P35557 [5-({3-isopropoxy-5-[2-(3-thienyl)ethoxy]benzoyl}amino)-1,3,4-thiadiazole-2-carboxylic acid]
ATP + D_Glucose -> ADP + D_Glucose_6_phosphate |
(( (Vmax * (S ^ n)) ) / (( (S_h ^ n) ) + (S ^ n))) |
|
buffer: 25 mM Hepes, 25 mM KCl, 5 mM mercaptoethanol, 1 mM NADP, 2.5 mM MgCl2, 2 units/ml glucose 6-phosphate dehydrogenase
PH: 7.1
Temperature: 22 |
uniprot:P35557 [5-({3-isopropoxy-5-[2-(3-thienyl)ethoxy]benzoyl}amino)-1,3,4-thiadiazole-2-carboxylic acid]
ATP + D_Glucose -> ADP + D_Glucose_6_phosphate |
(( (Vmax * (S ^ n)) ) / (( (S_h ^ n) ) + (S ^ n))) |
|
buffer: 25 mM Hepes, 25 mM KCl, 5 mM mercaptoethanol, 1 mM NADP, 2.5 mM MgCl2, 2 units/ml glucose 6-phosphate dehydrogenase
PH: 7.1
Temperature: 22 |
uniprot:P35557 [6-[(3isobutoxy-5-isopropoxybenzoyl)amino]nicotinic acid]
ATP + D_Glucose -> D_Glucose_6_phosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_h ^ n) ) + (S ^ n))) |
|
buffer: 25 mM Hepes, 25 mM KCl, 5 mM mercaptoethanol, 1 mM NADP, 2.5 mM MgCl2, 2 units/ml glucose 6-phosphate dehydrogenase
PH: 7.1
Temperature: 22 |
uniprot:P35557 [6-[(3isobutoxy-5-isopropoxybenzoyl)amino]nicotinic acid]
D_Glucose + ATP -> ADP + D_Glucose_6_phosphate |
(( (Vmax * (S ^ n)) ) / (( (S_h ^ n) ) + (S ^ n))) |
|
buffer: 25 mM Hepes, 25 mM KCl, 5 mM mercaptoethanol, 1 mM NADP, 2.5 mM MgCl2, 2 units/ml glucose 6-phosphate dehydrogenase
PH: 7.1
Temperature: 22 |
uniprot:P35557 [5-({3-isopropoxy-5-[2-(3-thienyl)ethoxy]benzoyl}amino)-1,3,4-thiadiazole-2-carboxylic acid]
D_Fructose + ATP -> D_Fructose_6_phosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_h ^ n) ) + (S ^ n))) |
|
buffer: 25 mM Hepes, 25 mM KCl, 5 mM mercaptoethanol, 1 mM NADP, 2.5 mM MgCl2, 2 units/ml glucose 6-phosphate dehydrogenase, 10 µg/ml phosphoglucoisomerase
PH: 7.1
Temperature: 22 |
uniprot:P35557 [D-Glucose]
D_Fructose + ATP -> D_Fructose_6_phosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_h ^ n) ) + (S ^ n))) |
|
buffer: 25 mM Hepes, 25 mM KCl, 5 mM mercaptoethanol, 1 mM NADP, 2.5 mM MgCl2, 2 units/ml glucose 6-phosphate dehydrogenase, 10 µg/ml phosphoglucoisomerase
PH: 7.1
Temperature: 22 |
uniprot:P35557 [glucokinase(Enzyme) wildtype]
ATP + D_Glucose -> D_Glucose_6_phosphate + ADP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 25 mM Hepes, 25 mM KCl, 5 mM mercaptoethanol, 1 mM NADP, 2.5 mM MgCl2, 2 units/ml glucose 6-phosphate dehydrogenase
PH: 7.1
Temperature: 22 |
uniprot:P35557 [6-[(3isobutoxy-5-isopropoxybenzoyl)amino]nicotinic acid]
D_Fructose + ATP -> ADP + D_Fructose_6_phosphate |
(( (Vmax * (S ^ n)) ) / (( (S_h ^ n) ) + (S ^ n))) |
|
buffer: 25 mM Hepes, 25 mM KCl, 5 mM mercaptoethanol, 1 mM NADP, 2.5 mM MgCl2, 2 units/ml glucose 6-phosphate dehydrogenase, 10 µg/ml phosphoglucoisomerase
PH: 7.1
Temperature: 22 |
uniprot:P35557 [glucokinase(Enzyme) wildtype]
D_Fructose + ATP -> D_Fructose_6_phosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_h ^ n) ) + (S ^ n))) |
|
buffer: 25 mM Hepes, 25 mM KCl, 5 mM mercaptoethanol, 1 mM NADP, 2.5 mM MgCl2, 2 units/ml glucose 6-phosphate dehydrogenase, 10 µg/ml phosphoglucoisomerase
PH: 7.1
Temperature: 22 |
uniprot:P35557 [6-[(3isobutoxy-5-isopropoxybenzoyl)amino]nicotinic acid]
D_Glucose + ATP -> D_Glucose_6_phosphate + ADP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 25 mM Hepes, 25 mM KCl, 5 mM mercaptoethanol, 1 mM NADP, 2.5 mM MgCl2, 2 units/ml glucose 6-phosphate dehydrogenase
PH: 7.1
Temperature: 22 |
uniprot:P35557 [6-[(3isobutoxy-5-isopropoxybenzoyl)amino]nicotinic acid]
ATP + D_Glucose -> D_Glucose_6_phosphate + ADP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 25 mM Hepes, 25 mM KCl, 5 mM mercaptoethanol, 1 mM NADP, 2.5 mM MgCl2, 2 units/ml glucose 6-phosphate dehydrogenase
PH: 7.1
Temperature: 22 |
uniprot:P17712 [glucokinase(Enzyme) wildtype]
ATP + D_Glucose -> ADP + D_Glucose_6_phosphate |
(( (V * (S - (P / Keq))) ) / (Ks * (( (1 + (S / Ks)) ) + (P / Kp)))) |
|
buffer: 50 mM Hepes / 10 mM sodium phosphate, 100 mM KCl, 10 mM MgCl2, 1 mg/ml trypsin inhibitor, 0.28 mM NADH, 2.5 mM phosphocreatine, 15 µM fructose 2,6-bisphosphate, 5 U/ml creatine kinase, 1 U/ml fructose-bisphosphate aldolase, 5 U/ml triose-phosphate isomerase, 3 U/ml glycerol 3-phosphate dehydrogenase
PH: 7.4
Temperature: 35 |
uniprot:P35557 [glucokinase(Enzyme) wildtype]
D_Glucose + ATP -> ADP + D_Glucose_6_phosphate |
(( (( (E * kcat) ) * (S ^ n)) ) / (( (S_h ^ n) ) + (S ^ n))) |
|
buffer: 100 mM Tris, 150 mM KCl, 6 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 % BSA, 0.4 mM NADP+, 0.2 U/ml glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant V62A]
D_Glucose + ATP -> ADP + D_Glucose_6_phosphate |
(( (( (E * kcat) ) * (S ^ n)) ) / (( (S_h ^ n) ) + (S ^ n))) |
|
buffer: 100 mM Tris, 150 mM KCl, 6 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 % BSA, 0.4 mM NADP+, 0.2 U/ml glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant V62E]
ATP + D_Glucose -> ADP + D_Glucose_6_phosphate |
(( (( (E * kcat) ) * (S ^ n)) ) / (( (S_h ^ n) ) + (S ^ n))) |
|
buffer: 100 mM Tris, 150 mM KCl, 6 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 % BSA, 0.4 mM NADP+, 0.2 U/ml glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant V62E]
D_Glucose + ATP -> D_Glucose_6_phosphate + ADP |
(( (( (E * kcat) ) * (S ^ n)) ) / (( (S_h ^ n) ) + (S ^ n))) |
|
buffer: 100 mM Tris, 150 mM KCl, 6 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 % BSA, 0.4 mM NADP+, 0.2 U/ml glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant V62A]
ATP + D_Glucose -> ADP + D_Glucose_6_phosphate |
(( (( (E * kcat) ) * (S ^ n)) ) / (( (S_h ^ n) ) + (S ^ n))) |
|
buffer: 100 mM Tris, 150 mM KCl, 6 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 % BSA, 0.4 mM NADP+, 0.2 U/ml glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant V62M]
D_Glucose + ATP -> D_Glucose_6_phosphate + ADP |
(( (( (E * kcat) ) * (S ^ n)) ) / (( (S_h ^ n) ) + (S ^ n))) |
|
buffer: 100 mM Tris, 150 mM KCl, 6 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 % BSA, 0.4 mM NADP+, 0.2 U/ml glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant V62L]
ATP + D_Glucose -> ADP + D_Glucose_6_phosphate |
(( (( (E * kcat) ) * (S ^ n)) ) / (( (S_h ^ n) ) + (S ^ n))) |
|
buffer: 100 mM Tris, 150 mM KCl, 6 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 % BSA, 0.4 mM NADP+, 0.2 U/ml glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant V62K]
D_Glucose + ATP -> D_Glucose_6_phosphate + ADP |
(( (( (E * kcat) ) * (S ^ n)) ) / (( (S_h ^ n) ) + (S ^ n))) |
|
buffer: 100 mM Tris, 150 mM KCl, 6 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 % BSA, 0.4 mM NADP+, 0.2 U/ml glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant V62Q]
D_Glucose + ATP -> D_Glucose_6_phosphate + ADP |
(( (( (E * kcat) ) * (S ^ n)) ) / (( (S_h ^ n) ) + (S ^ n))) |
|
buffer: 100 mM Tris, 150 mM KCl, 6 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 % BSA, 0.4 mM NADP+, 0.2 U/ml glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant V62L]
ATP + D_Glucose -> D_Glucose_6_phosphate + ADP |
(( (( (E * kcat) ) * (S ^ n)) ) / (( (S_h ^ n) ) + (S ^ n))) |
|
buffer: 100 mM Tris, 150 mM KCl, 6 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 % BSA, 0.4 mM NADP+, 0.2 U/ml glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant V62K]
ATP + D_Glucose -> D_Glucose_6_phosphate + ADP |
(( (( (E * kcat) ) * (S ^ n)) ) / (( (S_h ^ n) ) + (S ^ n))) |
|
buffer: 100 mM Tris, 150 mM KCl, 6 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 % BSA, 0.4 mM NADP+, 0.2 U/ml glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant V62Q]
ATP + D_Glucose -> ADP + D_Glucose_6_phosphate |
(( (( (E * kcat) ) * (S ^ n)) ) / (( (S_h ^ n) ) + (S ^ n))) |
|
buffer: 100 mM Tris, 150 mM KCl, 6 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 % BSA, 0.4 mM NADP+, 0.2 U/ml glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant V62F]
ATP + D_Glucose -> ADP + D_Glucose_6_phosphate |
(( (( (E * kcat) ) * (S ^ n)) ) / (( (S_h ^ n) ) + (S ^ n))) |
|
buffer: 100 mM Tris, 150 mM KCl, 6 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 % BSA, 0.4 mM NADP+, 0.2 U/ml glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant V62F]
ATP + D_Glucose -> D_Glucose_6_phosphate + ADP |
(( (( (E * kcat) ) * (S ^ n)) ) / (( (S_h ^ n) ) + (S ^ n))) |
|
buffer: 100 mM Tris, 150 mM KCl, 6 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 % BSA, 0.4 mM NADP+, 0.2 U/ml glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant V62T]
ATP + D_Glucose -> D_Glucose_6_phosphate + ADP |
(( (( (E * kcat) ) * (S ^ n)) ) / (( (S_h ^ n) ) + (S ^ n))) |
|
buffer: 100 mM Tris, 150 mM KCl, 6 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 % BSA, 0.4 mM NADP+, 0.2 U/ml glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant V62T]
D_Glucose + ATP -> D_Glucose_6_phosphate + ADP |
(( (( (E * kcat) ) * (S ^ n)) ) / (( (S_h ^ n) ) + (S ^ n))) |
|
buffer: 100 mM Tris, 150 mM KCl, 6 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 % BSA, 0.4 mM NADP+, 0.2 U/ml glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) wildtype]
ATP + D_Glucose -> D_Glucose_6_phosphate + ADP |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 100 mM Tris, 150 mM KCl, 6 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 % BSA, 0.4 mM NADP+, 0.2 U/ml glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant V62F]
D_Glucose + ATP -> D_Glucose_6_phosphate + ADP |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 100 mM Tris, 150 mM KCl, 6 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 % BSA, 0.4 mM NADP+, 0.2 U/ml glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant V62A]
ATP + D_Glucose -> ADP + D_Glucose_6_phosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 100 mM Tris, 150 mM KCl, 6 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 % BSA, 0.4 mM NADP+, 0.2 U/ml glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant V62E]
ATP + D_Glucose -> ADP + D_Glucose_6_phosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 100 mM Tris, 150 mM KCl, 6 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 % BSA, 0.4 mM NADP+, 0.2 U/ml glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant V62F]
D_Glucose + ATP -> D_Glucose_6_phosphate + ADP |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 100 mM Tris, 150 mM KCl, 6 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 % BSA, 0.4 mM NADP+, 0.2 U/ml glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant V62A]
ATP + D_Glucose -> D_Glucose_6_phosphate + ADP |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 100 mM Tris, 150 mM KCl, 6 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 % BSA, 0.4 mM NADP+, 0.2 U/ml glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant V62E]
ATP + D_Glucose -> D_Glucose_6_phosphate + ADP |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 100 mM Tris, 150 mM KCl, 6 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 % BSA, 0.4 mM NADP+, 0.2 U/ml glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant V62M]
D_Glucose + ATP -> D_Glucose_6_phosphate + ADP |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 100 mM Tris, 150 mM KCl, 6 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 % BSA, 0.4 mM NADP+, 0.2 U/ml glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant V62K]
D_Glucose + ATP -> ADP + D_Glucose_6_phosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 100 mM Tris, 150 mM KCl, 6 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 % BSA, 0.4 mM NADP+, 0.2 U/ml glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant V62K]
D_Glucose + ATP -> ADP + D_Glucose_6_phosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 100 mM Tris, 150 mM KCl, 6 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 % BSA, 0.4 mM NADP+, 0.2 U/ml glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant V62L]
D_Glucose + ATP -> D_Glucose_6_phosphate + ADP |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 100 mM Tris, 150 mM KCl, 6 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 % BSA, 0.4 mM NADP+, 0.2 U/ml glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant V62T]
ATP + D_Glucose -> ADP + D_Glucose_6_phosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 100 mM Tris, 150 mM KCl, 6 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 % BSA, 0.4 mM NADP+, 0.2 U/ml glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant V62L]
D_Glucose + ATP -> ADP + D_Glucose_6_phosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 100 mM Tris, 150 mM KCl, 6 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 % BSA, 0.4 mM NADP+, 0.2 U/ml glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant V62Q]
ATP + D_Glucose -> D_Glucose_6_phosphate + ADP |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 100 mM Tris, 150 mM KCl, 6 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 % BSA, 0.4 mM NADP+, 0.2 U/ml glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant V62T]
ATP + D_Glucose -> ADP + D_Glucose_6_phosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 100 mM Tris, 150 mM KCl, 6 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 % BSA, 0.4 mM NADP+, 0.2 U/ml glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant V62Q]
D_Glucose + ATP -> D_Glucose_6_phosphate + ADP |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 100 mM Tris, 150 mM KCl, 6 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 % BSA, 0.4 mM NADP+, 0.2 U/ml glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
uniprot:P0A6V8 [glucokinase(Enzyme) wildtype His-tagged]
ATP + Glucose -> Glucose_6_phosphate + ADP |
(( (( (Vmax * A) ) * B) ) / (( (( (( (KmA * KmB) ) + (KmB * A)) ) + (KmA * B)) ) + (A * B))) |
|
buffer: 100 mM MOPS, 2 mM DTT, 8 U/ml G6PDH, 1 mM NADP, 20 mM MgCl2
PH: 7.2
Temperature: 37 |
uniprot:P17712 [glucokinase(Enzyme) wildtype]
ATP + Glucose -> ADP + Glucose_6_phosphate |
(( (Vmax * (S1 ^ n)) ) / (( (S1_half ^ n) ) + (S1 ^ n))) |
|
buffer: 25 mM Hepes, 1 mM dithiothreitol, 200 µM fructose 6-phosphate, 25 mM KCl, 0.15 mM NADH, 0.25 mM phosphoenolpyruvate, 10 µg/ml rabbit muscle lactate dehydrogenase, 10 µg/ml rabbit muscle pyruvate kinase
PH: 7.1
Temperature: 30 |
uniprot:P17712 [glucokinase(Enzyme) wildtype]
ATP + Glucose -> Glucose_6_phosphate + ADP |
(( (Vmax * (S1 ^ n)) ) / (( (S1_half ^ n) ) + (S1 ^ n))) |
|
buffer: 25 mM Hepes, 1 mM dithiothreitol, 200 µM fructose 6-phosphate, 25 mM KCl, 0.15 mM NADH, 0.25 mM phosphoenolpyruvate, 10 µg/ml rabbit muscle lactate dehydrogenase, 10 µg/ml rabbit muscle pyruvate kinase
PH: 6.55
Temperature: 30 |
uniprot:P17712 [glucokinase(Enzyme) wildtype]
ATP + Glucose -> Glucose_6_phosphate + ADP |
(( (Vmax * (S1 ^ n)) ) / (( (S1_half ^ n) ) + (S1 ^ n))) |
|
buffer: 25 mM Hepes, 1 mM dithiothreitol, 200 µM fructose 6-phosphate, 25 mM KCl, 0.15 mM NADH, 0.25 mM phosphoenolpyruvate, 10 µg/ml rabbit muscle lactate dehydrogenase, 10 µg/ml rabbit muscle pyruvate kinase
PH: 8.15
Temperature: 30 |
uniprot:P17712 [glucokinase(Enzyme) wildtype]
ATP + D_Glucose -> D_Glucose_6_phosphate + ADP |
(( (Vmax * S1) ) / (Km1 + S1)) |
|
buffer: Krebs-Ringer bicarbonate, 10 mM Hepes, 10 mM NaF
PH: 7.4
Temperature: 37 |
uniprot:P17712 [glucokinase(Enzyme) wildtype]
D_Glucose + ATP -> D_Glucose_6_phosphate + ADP |
(( (Vmax * S1) ) / (Km1 + S1)) |
|
buffer: Krebs-Ringer bicarbonate, 10 mM Hepes, 10 mM NaF
PH: 7.4
Temperature: 37 |
uniprot:P17712 [glucokinase(Enzyme) wildtype]
ATP + Glucose -> Glucose_6_phosphate + ADP |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 50 mM Tetramethylammonium glycylglycinate, 100 mM KCl, 0.25 mM NADH, 1 mM MgCl2, 13.3 units/ml Pyruvate kinase, 13.3 units/ml Lactate dehydrogenase, 1 mM Phosphoenolpyruvate
PH: 8
Temperature: 30 |
uniprot:P17712 [glucokinase(Enzyme) wildtype type IV]
D_Glucose + ATP -> ADP + D_Glucose_6_phosphate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 50 mM Tris, 0.25 mM NADP, 5 mM MgCl2, 5 mM beta-Mercaptoethanol, 0.1 unit/ml Glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 21 |
uniprot:P35557 [glucokinase(Enzyme) mutant Y214C]
D_Glucose + ATP -> Glucose_6_phosphate + ADP |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM HEPES, 3 mM MgCl2, 25 mM KCl, 0.7 mM NADH, 2 mM DTT, 4 mM PEP, 1 unit/ml pyruvate kinase, 1 unit/ml lactate dehydrogenase
PH: 8
Temperature: 25 |
uniprot:P35557 [glucokinase(Enzyme) mutant Y214C]
ATP + D_Glucose -> ADP + Glucose_6_phosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM HEPES, 3 mM MgCl2, 25 mM KCl, 0.7 mM NADH, 2 mM DTT, 4 mM PEP, 1 unit/ml pyruvate kinase, 1 unit/ml lactate dehydrogenase
PH: 8
Temperature: 25 |
uniprot:P35557 [glucokinase(Enzyme) mutant Y214C]
ATP + D_Glucose -> Glucose_6_phosphate + ADP |
(( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM HEPES, 3 mM MgCl2, 25 mM KCl, 0.7 mM NADH, 2 mM DTT, 4 mM PEP, 1 unit/ml pyruvate kinase, 1 unit/ml lactate dehydrogenase
PH: 8
Temperature: 25 |
uniprot:P35557 [glucokinase(Enzyme) wildtype]
2_Deoxy_D_glucose + ATP -> ADP + 2_Deoxy_D_glucose_6_phosphate |
(( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM HEPES, 3 mM MgCl2, 25 mM KCl, 0.7 mM NADH, 2 mM DTT, 4 mM PEP, 1 unit/ml pyruvate kinase, 1 unit/ml lactate dehydrogenase
PH: 8
Temperature: 25 |
uniprot:P0A6V8 [glucokinase(Enzyme) wildtype]
D_Glucose + ATP -> D_Glucose_6_phosphate + ADP |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.3-0.5 M Tris-HCl
PH: 7.6
Temperature: 25 |
uniprot:P0A6V8 [glucokinase(Enzyme) wildtype]
D_Glucose + ATP -> D_Glucose_6_phosphate + ADP |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.3-0.5 M Tris-HCl
PH: 7.6
Temperature: 25 |
uniprot:P35557 [glucokinase(Enzyme) wildtype]
D_Glucose + ATP -> ADP + Glucose_6_phosphate |
(( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM HEPES, 3 mM MgCl2, 25 mM KCl, 0.7 mM NADH, 2 mM DTT, 4 mM PEP, 1 unit/ml pyruvate kinase, 1 unit/ml lactate dehydrogenase
PH: 8
Temperature: 25 |
uniprot:P35557 [glucokinase(Enzyme) mutant Y214C]
ATP + D_Glucose -> ADP + Glucose_6_phosphate |
(( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM HEPES, 3 mM MgCl2, 25 mM KCl, 0.7 mM NADH, 2 mM DTT, 4 mM PEP, 1 unit/ml pyruvate kinase, 1 unit/ml lactate dehydrogenase
PH: 8
Temperature: 25 |
uniprot:P35557 [glucokinase(Enzyme) wildtype]
ATP + D_Glucose -> ADP + Glucose_6_phosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM HEPES, 3 mM MgCl2, 25 mM KCl, 0.7 mM NADH, 2 mM DTT, 4 mM PEP, 1 unit/ml pyruvate kinase, 1 unit/ml lactate dehydrogenase
PH: 8
Temperature: 25 |
uniprot:P35557 [glucokinase(Enzyme) wildtype]
D_Glucose + ATP -> Glucose_6_phosphate + ADP |
(( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM HEPES, 3 mM MgCl2, 25 mM KCl, 0.7 mM NADH, 2 mM DTT, 4 mM PEP, 1 unit/ml pyruvate kinase, 1 unit/ml lactate dehydrogenase
PH: 8
Temperature: 25 |
uniprot:P35557 [glucokinase(Enzyme) wildtype]
ATP + D_Glucose -> ADP + Glucose_6_phosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM HEPES, 3 mM MgCl2, 25 mM KCl, 0.7 mM NADH, 2 mM DTT, 4 mM PEP, 1 unit/ml pyruvate kinase, 1 unit/ml lactate dehydrogenase
PH: 8
Temperature: 25 |
uniprot:P35557 [glucokinase(Enzyme) wildtype]
2_Deoxy_D_glucose + ATP -> ADP + 2_Deoxy_D_glucose_6_phosphate |
(( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM HEPES, 3 mM MgCl2, 25 mM KCl, 0.7 mM NADH, 2 mM DTT, 4 mM PEP, 1 unit/ml pyruvate kinase, 1 unit/ml lactate dehydrogenase
PH: 8
Temperature: 25 |
uniprot:P35557 [glucokinase(Enzyme) mutant fusion protein to glutathione S-transferase A208G]
ATP + D_Glucose -> D_Glucose_6_phosphate + ADP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM Tris, 6 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 % BSA, 150 mM KCl, 0.4 mM NADP+, 0.2 U/ml glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) wildtype fusion protein to glutathione S-transferase]
D_Glucose + ATP -> D_Glucose_6_phosphate + ADP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM Tris, 6 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 % BSA, 150 mM KCl, 0.4 mM NADP+, 0.2 U/ml glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant fusion protein to glutathione S-transferase K459L]
D_Glucose + ATP -> ADP + D_Glucose_6_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM Tris, 6 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 % BSA, 150 mM KCl, 0.4 mM NADP+, 0.2 U/ml glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant fusion protein to glutathione S-transferase Y215A]
ATP + D_Glucose -> D_Glucose_6_phosphate + ADP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM Tris, 6 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 % BSA, 150 mM KCl, 0.4 mM NADP+, 0.2 U/ml glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant fusion protein to glutathione S-transferase A460R]
D_Glucose + ATP -> ADP + D_Glucose_6_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM Tris, 6 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 % BSA, 150 mM KCl, 0.4 mM NADP+, 0.2 U/ml glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant fusion protein to glutathione S-transferase C457V]
ATP + D_Glucose -> ADP + D_Glucose_6_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM Tris, 6 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 % BSA, 150 mM KCl, 0.4 mM NADP+, 0.2 U/ml glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant fusion protein to glutathione S-transferase A456V]
D_Glucose + ATP -> D_Glucose_6_phosphate + ADP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM Tris, 6 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 % BSA, 150 mM KCl, 0.4 mM NADP+, 0.2 U/ml glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant fusion protein to glutathione S-transferase Y215C]
ATP + D_Glucose -> ADP + D_Glucose_6_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM Tris, 6 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 % BSA, 150 mM KCl, 0.4 mM NADP+, 0.2 U/ml glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant fusion protein to glutathione S-transferase K458R]
D_Glucose + ATP -> ADP + D_Glucose_6_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM Tris, 6 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 % BSA, 150 mM KCl, 0.4 mM NADP+, 0.2 U/ml glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
uniprot:P52792 [glucokinase(Enzyme) wildtype]
D_Glucose + ATP -> D_Glucose_6_phosphate + ADP |
(( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM HEPES, 100 mM KCl, 7.5 mM MgCl2, 2.5 mM dithiothreitol, 0.5 mM NAD+, glucose-6-phosphate dehydrogenase
PH: 7.4
Temperature: 37 |
uniprot:P52792 [glucokinase(Enzyme) mutant A456V (GST-GK)]
ATP + D_Glucose -> ADP + D_Glucose_6_phosphate |
(( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM HEPES, 100 mM KCl, 7.5 mM MgCl2, 2.5 mM dithiothreitol, 0.5 mM NAD+, glucose-6-phosphate dehydrogenase
PH: 7.4
Temperature: 37 |
uniprot:P52792 [glucokinase(Enzyme) mutant K414E (GST-GK)]
D_Glucose + ATP -> ADP + D_Glucose_6_phosphate |
(( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM HEPES, 100 mM KCl, 7.5 mM MgCl2, 2.5 mM dithiothreitol, 0.5 mM NAD+, glucose-6-phosphate dehydrogenase
PH: 7.4
Temperature: 37 |
uniprot:P52792 [glucokinase(Enzyme) wildtype]
D_Glucose + ATP -> ADP + D_Glucose_6_phosphate |
(( (( (kcat * E) ) * A) ) / (Km + A)) |
|
buffer: 50 mM HEPES, 100 mM KCl, 7.5 mM MgCl2, 2.5 mM dithiothreitol, 0.5 mM NAD+, glucose-6-phosphate dehydrogenase
PH: 7.4
Temperature: 37 |
uniprot:P52792 [glucokinase(Enzyme) mutant A456V (GST-GK)]
D_Glucose + ATP -> D_Glucose_6_phosphate + ADP |
(( (( (kcat * E) ) * A) ) / (Km + A)) |
|
buffer: 50 mM HEPES, 100 mM KCl, 7.5 mM MgCl2, 2.5 mM dithiothreitol, 0.5 mM NAD+, glucose-6-phosphate dehydrogenase
PH: 7.4
Temperature: 37 |
uniprot:P52792 [glucokinase(Enzyme) mutant K414E (GST-GK)]
ATP + D_Glucose -> ADP + D_Glucose_6_phosphate |
(( (( (kcat * E) ) * A) ) / (Km + A)) |
|
buffer: 50 mM HEPES, 100 mM KCl, 7.5 mM MgCl2, 2.5 mM dithiothreitol, 0.5 mM NAD+, glucose-6-phosphate dehydrogenase
PH: 7.4
Temperature: 37 |
uniprot:P35557 [glucokinase(Enzyme) wildtype fusion protein to glutathione S-transferase]
D_Glucose + ATP -> ADP + D_Glucose_6_phosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 100 mM Tris, 6 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 % BSA, 150 mM KCl, 0.4 mM NADP+, 0.2 U/ml glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant fusion protein to glutathione S-transferase C457V]
ATP + D_Glucose -> ADP + D_Glucose_6_phosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 100 mM Tris, 6 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 % BSA, 150 mM KCl, 0.4 mM NADP+, 0.2 U/ml glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant fusion protein to glutathione S-transferase K459L]
D_Glucose + ATP -> ADP + D_Glucose_6_phosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 100 mM Tris, 6 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 % BSA, 150 mM KCl, 0.4 mM NADP+, 0.2 U/ml glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant fusion protein to glutathione S-transferase A208G]
ATP + D_Glucose -> ADP + D_Glucose_6_phosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 100 mM Tris, 6 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 % BSA, 150 mM KCl, 0.4 mM NADP+, 0.2 U/ml glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant fusion protein to glutathione S-transferase Y215A]
D_Glucose + ATP -> ADP + D_Glucose_6_phosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 100 mM Tris, 6 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 % BSA, 150 mM KCl, 0.4 mM NADP+, 0.2 U/ml glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant fusion protein to glutathione S-transferase A460R]
ATP + D_Glucose -> ADP + D_Glucose_6_phosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 100 mM Tris, 6 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 % BSA, 150 mM KCl, 0.4 mM NADP+, 0.2 U/ml glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant fusion protein to glutathione S-transferase A456V]
D_Glucose + ATP -> ADP + D_Glucose_6_phosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 100 mM Tris, 6 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 % BSA, 150 mM KCl, 0.4 mM NADP+, 0.2 U/ml glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant fusion protein to glutathione S-transferase Y214C]
D_Glucose + ATP -> D_Glucose_6_phosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 100 mM Tris, 6 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 % BSA, 150 mM KCl, 0.4 mM NADP+, 0.2 U/ml glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
uniprot:P35557 [glucokinase(Enzyme) mutant fusion protein to glutathione S-transferase K458R]
ATP + D_Glucose -> ADP + D_Glucose_6_phosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 100 mM Tris, 6 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 % BSA, 150 mM KCl, 0.4 mM NADP+, 0.2 U/ml glucose 6-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
uniprot:P0A6V8 [glucokinase(Enzyme) wildtype]
ATP + D_Glucose -> D_Glucose_6_phosphate + ADP |
(( (Vmax * S1) ) / (Km + S1)) |
|
buffer: 0.05 M Tris, 2 mM MgCl2, 2 mM NADP+, 0.1 ug/ml glucose-6-P dehydrogenase
PH: 7.65
Temperature: 36 |
uniprot:P0A6V8 [glucokinase(Enzyme) wildtype]
D_Glucose + ATP -> D_Glucose_6_phosphate + ADP |
(( (Vmax * S2) ) / (Km + S2)) |
|
buffer: 0.05 M Tris, 2 mM MgCl2, 2 mM NADP+, 0.1 ug/ml glucose-6-P dehydrogenase
PH: 7.65
Temperature: 36 |
uniprot:P17712 [glucokinase(Enzyme) wildtype]
ATP + D_Glucose -> D_Glucose_6_phosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 10 mM HEPES, 100 mM KCl, 2 mM Mg2+, 0,25 mM Phosphoenolpyruvate, 0.15 mM NADH, 1 mM KH2PO4, 4 U/ml pyruvate kinase, 10 U/ml lactate dehydrogenase
PH: 7.5
Temperature: 36 |
uniprot:P17712 [Sorbitol]
D_Glucose + ATP -> ADP + D_Glucose_6_phosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: Minimum Essential Medium (MEM), 7 % neonatal calf serum
PH: 7.5
Temperature: 37 |
uniprot:P17712 [glucokinase(Enzyme) wildtype]
D_Glucose + ATP -> ADP + D_Glucose_6_phosphate |
(( (Vmax * (E ^ n)) ) / (( (E_half ^ n) ) + (E ^ n))) |
|
buffer: Minimum Essential Medium (MEM), 7 % neonatal calf serum, 2 µM cycloheximide
PH: 7.5
Temperature: 37 |
uniprot:P17712 [glucokinase(Enzyme) wildtype]
D_Glucose + ATP -> D_Glucose_6_phosphate + ADP |
(( (Vmax * (E ^ n)) ) / (( (E_half ^ n) ) + (E ^ n))) |
|
buffer: Minimum Essential Medium (MEM), 7 % neonatal calf serum, 2 µM cycloheximide
PH: 7.5
Temperature: 37 |
uniprot:P17712 [glucokinase(Enzyme) wildtype]
ATP + D_Glucose -> ADP + D_Glucose_6_phosphate |
(( (Vmax * (E ^ n)) ) / (( (E_half ^ n) ) + (E ^ n))) |
|
buffer: Minimum Essential Medium (MEM), 7 % neonatal calf serum, 2 µM cycloheximide
PH: 7.5
Temperature: 37 |
uniprot:P17712 [glucokinase(Enzyme) wildtype]
ATP + D_Glucose -> D_Glucose_6_phosphate + ADP |
(( (Vmax * (E ^ n)) ) / (( (E_half ^ n) ) + (E ^ n))) |
|
buffer: Minimum Essential Medium (MEM), 7 % neonatal calf serum, 2 µM cycloheximide
PH: 7.5
Temperature: 37 |
uniprot:P17712 [glucokinase(Enzyme) wildtype]
ATP + D_Glucose -> ADP + D_Glucose_6_phosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: Minimum Essential Medium (MEM), 7 % neonatal calf serum
PH: 7.5
Temperature: 37 |
uniprot:P17712 [glucokinase(Enzyme) wildtype]
ATP + D_Mannose -> ADP + D_Mannose_6_phosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 10 mM HEPES, 100 mM KCl, 2 mM Mg2+, 0,25 mM Phosphoenolpyruvate, 0.15 mM NADH, 1 mM KH2PO4, 4 U/ml pyruvate kinase, 10 U/ml lactate dehydrogenase
PH: 7.5
Temperature: 36 |
uniprot:P17712 [glucokinase(Enzyme) wildtype]
ATP + 2_Deoxy_D_glucose -> 2_Deoxy_D_glucose_6_phosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 10 mM HEPES, 100 mM KCl, 2 mM Mg2+, 0,25 mM Phosphoenolpyruvate, 0.15 mM NADH, 1 mM KH2PO4, 4 U/ml pyruvate kinase, 10 U/ml lactate dehydrogenase
PH: 7.5
Temperature: 36 |