EC: 2.6.1.44

alanine-glyoxylate transaminase (L-alanine:glyoxylate aminotransferase)

enzyme lambda metabolic reaction experiment
uniprot:P43567 [alanine---glyoxylate transaminase(Enzyme) wildtype]

L_Alanine + Glyoxylate -> Pyruvate + Glycine		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 40 µM pyridoxal 5'-phosphate
PH: 8
Temperature: 37
uniprot:P43567 [alanine---glyoxylate transaminase(Enzyme) wildtype]

L_Alanine + Glyoxylate -> Pyruvate + Glycine		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Potassium phosphate, 40 µM Pyridoxal 5'-phosphate
PH: 8
Temperature: 37
uniprot:P09139 [Pyridoxal phosphate]

L_Alanine + Glyoxylate -> Pyruvate + Glycine		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Tricine-NaOH, 0.1 % Triton X-100
PH: 8
Temperature: 30
uniprot:P09139 [Pyridoxal phosphate]

Glyoxylate + L_Alanine -> Glycine + Pyruvate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Tricine-NaOH, 0.1 % Triton X-100
PH: 8
Temperature: 30

Pathways

pathway id name
BioCyc:YEAST_GLYSYN-ALA-PWY glycine biosynthesis from glyoxylate
BioCyc:HUMAN_GLYSYN-ALA-PWY glycine biosynthesis
BioCyc:META_PWY-8011 L-serine biosynthesis II
BioCyc:META_PWY-181 photorespiration
BioCyc:MOUSE_GLYSYN-ALA-PWY glycine biosynthesis III
BioCyc:ARA_PWY-3941 β-alanine biosynthesis II
BioCyc:META_GLYSYN-ALA-PWY glycine biosynthesis III
BioCyc:ARA_GLYSYN-ALA-PWY glycine biosynthesis III
PlantCyc:ARA_GLYSYN-ALA-PWY glycine biosynthesis III
PlantCyc:ARA_GLYSYN2-PWY glycine biosynthesis
PlantCyc:PLANT_PWY-8011 L-serine biosynthesis II
PlantCyc:ARA_PWY-8011 L-serine biosynthesis II
PlantCyc:PLANT_ALANINE-DEG3-PWY L-alanine degradation III
PlantCyc:PLANT_ALANINE-SYN2-PWY L-alanine biosynthesis II
PlantCyc:ARA_PWY-181 photorespiration
PlantCyc:ARA_PWY-3941 β-alanine biosynthesis II
PlantCyc:ARA_ALANINE-SYN2-PWY L-alanine biosynthesis II
PlantCyc:ARA_ALANINE-DEG3-PWY L-alanine degradation III
PlantCyc:ARA_PWY-4221 superpathway of coenzyme A biosynthesis II (plants)
PlantCyc:PLANT_PWY-181 photorespiration
PlantCyc:ARA_ALACAT2-PWY L-alanine degradation II (to D-lactate)
PlantCyc:PLANT_GLYSYN2-PWY glycine biosynthesis
Reactome:R-DME-389661 Glyoxylate metabolism and glycine degradation
Reactome:R-HSA-389661 Glyoxylate metabolism and glycine degradation
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-389661 Glyoxylate metabolism and glycine degradation
Reactome:R-RNO-389661 Glyoxylate metabolism and glycine degradation
Reactome:R-DDI-71291 Amino acid and derivative metabolism
Reactome:R-DDI-389661 Glyoxylate metabolism and glycine degradation
Reactome:R-DME-1430728 Metabolism
Reactome:R-DME-71291 Amino acid and derivative metabolism
Reactome:R-HSA-73621 Pyrimidine catabolism
Reactome:R-HSA-71291 Amino acid and derivative metabolism
Reactome:R-MMU-71291 Amino acid and derivative metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-71291 Amino acid and derivative metabolism
Reactome:R-DDI-1430728 Metabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-15869 Nucleotide metabolism
Reactome:R-HSA-8956319 Nucleotide catabolism
WikiPathways:WP5166 Glyoxylate metabolism
WikiPathways:WP4225 Pyrimidine metabolism and related diseases
PathBank:SMP0120504 Lactic Acidemia
PathBank:SMP0000055 Alanine Metabolism
PathBank:SMP0000242 Dimethylglycine Dehydrogenase Deficiency
PathBank:SMP0000223 Non-Ketotic Hyperglycinemia
PathBank:SMP0000484 Dimethylglycine Dehydrogenase Deficiency
PathBank:SMP0002420 Proline Metabolism
PathBank:SMP0120653 3-Phosphoglycerate Dehydrogenase Deficiency
PathBank:SMP0120475 Dimethylglycine Dehydrogenase Deficiency
PathBank:SMP0120535 Pyruvate Carboxylase Deficiency
PathBank:SMP0120543 Primary Hyperoxaluria Type I
PathBank:SMP0000004 Glycine and Serine Metabolism
PathBank:SMP0000721 3-Phosphoglycerate Dehydrogenase Deficiency
PathBank:SMP0002416 Arginine Metabolism
PathBank:SMP0002428 Glycine Metabolism
PathBank:SMP0120521 Sarcosinemia
PathBank:SMP0000352 Primary Hyperoxaluria Type I
PathBank:SMP0000179 Dihydropyrimidine Dehydrogenase Deficiency (DHPD)
PathBank:SMP0000485 Hyperglycinemia, Non-Ketotic
PathBank:SMP0002320 Glycine Metabolism
PathBank:SMP0087368 Alanine Metabolism
PathBank:SMP0063586 Alanine Metabolism
PathBank:SMP0120474 Dihydropyrimidine Dehydrogenase Deficiency (DHPD)
PathBank:SMP0120534 Non-Ketotic Hyperglycinemia
PathBank:SMP0120550 Hyperglycinemia, Non-Ketotic
PathBank:SMP0000350 Pyruvate Carboxylase Deficiency
PathBank:SMP0000313 Lactic Acidemia
PathBank:SMP0000244 Sarcosinemia
PathBank:SMP0002415 Alanine Metabolism
PathBank:SMP0087399 Glycine and Serine Metabolism
PathBank:SMP0063626 Glycine and Serine Metabolism