EC: 2.5.1.34

4-dimethylallyltryptophan synthase (dimethylallyl-diphosphate:L-tryptophan 4-dimethylallyltransferase)

enzyme lambda metabolic reaction experiment
uniprot:Q12594 [Ca2+]

L_Tryptophan + Dimethylallyl_diphosphate -> Diphosphate + 4_(3_Methylbut_2_enyl)_L_tryptophan
(( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 20 mM 2-mercaptoethanol, 10 % (v/v) glycerol
PH: 7.8
Temperature: 30
uniprot:Q12594 [4-dimethylallyltryptophan synthase(Enzyme) wildtype]

L_Tryptophan + Dimethylallyl_diphosphate -> Diphosphate + 4_(3_Methylbut_2_enyl)_L_tryptophan
(( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 20 mM 2-mercaptoethanol, 10 % (v/v) glycerol
PH: 7.8
Temperature: 30
uniprot:Q12594 [4-dimethylallyltryptophan synthase(Enzyme) wildtype]

L_Tryptophan + Dimethylallyl_diphosphate -> 4_(3_Methylbut_2_enyl)_L_tryptophan + Diphosphate
(( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 20 mM 2-mercaptoethanol, 10 % (v/v) glycerol
PH: 7.8
Temperature: 30
uniprot:Q12594 [Mg2+]

Dimethylallyl_diphosphate + L_Tryptophan -> 4_(3_Methylbut_2_enyl)_L_tryptophan + Diphosphate
(( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 20 mM 2-mercaptoethanol, 10 % (v/v) glycerol
PH: 7.8
Temperature: 30
uniprot:Q12594 [Mg2+]

L_Tryptophan + Dimethylallyl_diphosphate -> 4_(3_Methylbut_2_enyl)_L_tryptophan + Diphosphate
(( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 20 mM 2-mercaptoethanol, 10 % (v/v) glycerol
PH: 7.8
Temperature: 30
uniprot:Q12594 [Ca2+]

Dimethylallyl_diphosphate + L_Tryptophan -> 4_(3_Methylbut_2_enyl)_L_tryptophan + Diphosphate
(( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 20 mM 2-mercaptoethanol, 10 % (v/v) glycerol
PH: 7.8
Temperature: 30
uniprot:Q50EL0 [Ca2+]

L_Tryptophan + Dimethylallyl_diphosphate -> Diphosphate + 4_(3_Methylbut_2_enyl)_L_tryptophan
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris/HCl
PH: 7.5
Temperature: 30
uniprot:Q50EL0 [Ca2+]

Dimethylallyl_diphosphate + L_Tryptophan -> 4_(3_Methylbut_2_enyl)_L_tryptophan + Diphosphate
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris/HCl
PH: 7.5
Temperature: 30
uniprot:Q12594 [Ca2+]

Dimethylallyl_diphosphate + L_Tryptophan -> 4_(3_Methylbut_2_enyl)_L_tryptophan + Diphosphate
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 10 mM Tris-HCl, 20 mM 2-Mercaptoethanol
PH: 7.5
Temperature: 30
uniprot:Q12594 [Ca2+]

Dimethylallyl_diphosphate + L_Tryptophan -> 4_(3_Methylbut_2_enyl)_L_tryptophan + Diphosphate
(( (Vmax * A) ) / (Km + A)) buffer: 10 mM Tris-HCl, 20 mM 2-Mercaptoethanol
PH: 7.5
Temperature: 30
uniprot:Q12594 [Ca2+]

Dimethylallyl_diphosphate + L_Tryptophan -> 4_(3_Methylbut_2_enyl)_L_tryptophan + Diphosphate
(( (Vmax * A) ) / (Km + A)) buffer: 10 mM Tris-HCl, 20 mM 2-Mercaptoethanol
PH: 7.5
Temperature: 30
uniprot:Q12594 [4-dimethylallyltryptophan synthase(Enzyme) wildtype]

Dimethylallyl_diphosphate + L_Tryptophan -> 4_(3_Methylbut_2_enyl)_L_tryptophan + Diphosphate
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 10 mM Tris-HCl, 20 mM 2-Mercaptoethanol
PH: 7.5
Temperature: 30
uniprot:Q12594 [4-dimethylallyltryptophan synthase(Enzyme) wildtype]

Dimethylallyl_diphosphate + L_Tryptophan -> 4_(3_Methylbut_2_enyl)_L_tryptophan + Diphosphate
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 10 mM Tris-HCl, 20 mM 2-Mercaptoethanol
PH: 7.5
Temperature: 30

Pathways

pathway id name
BioCyc:META_PWY-6493 chanoclavine I aldehyde biosynthesis
BioCyc:META_PWY-6503 superpathway of ergotamine biosynthesis