EC: 2.5.1.15

dihydropteroate synthase ((7,8-dihydropterin-6-yl)methyl diphosphate:4-aminobenzoate 2-amino-4-hydroxy-7,8-dihydropteridine-6-methenyltransferase)

enzyme lambda metabolic reaction experiment
uniprot:P0C0G1 [dihydropteroate synthase(Enzyme) wildtype]

2_Amino_4_hydroxy_6_hydroxymethyl_7,8_dihydropteridine_diphosphate + 4_Aminobenzoate -> Diphosphate + Dihydropteroate		 (( (V * A) ) / (Km + A)) buffer: 0.1 M Tris-HCl, 0.1 M 2-mercaptoethanol, 10 mM MgCl2
PH: 8.4
Temperature: 30
uniprot:P0C0G0 [dihydropteroate synthase(Enzyme) wildtype]

2_Amino_4_hydroxy_6_hydroxymethyl_7,8_dihydropteridine_diphosphate + 4_Aminobenzoate -> Diphosphate + Dihydropteroate		 (( (V * A) ) / (Km + A)) buffer: 0.1 M Tris-HCl, 0.1 M 2-mercaptoethanol, 10 mM MgCl2
PH: 8.4
Temperature: 30
uniprot:P0C0X1 [dihydropteroate synthase(Enzyme) wildtype]

4_Aminobenzoate + 2_Amino_4_hydroxy_6_hydroxymethyl_7,8_dihydropteridine_diphosphate -> Dihydropteroate + Diphosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 5 mM DTT, 100 µg/ml BSA
PH: 8.3
Temperature: 37
uniprot:P9WND1 [dihydropteroate synthase(Enzyme) wildtype]

4_Aminobenzoate + 2_Amino_4_hydroxy_6_hydroxymethyl_7,8_dihydropteridine_diphosphate -> Dihydropteroate + Diphosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 5 mM DTT, 100 µg/ml BSA
PH: 8.3
Temperature: 37
uniprot:P9WND1 [dihydropteroate synthase(Enzyme) wildtype]

4_Aminobenzoate + 2_Amino_4_hydroxy_6_hydroxymethyl_7,8_dihydropteridine_diphosphate -> Dihydropteroate + Diphosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 5 mM DTT, 100 µg/ml BSA
PH: 8.3
Temperature: 37
uniprot:P0C0X1 [dihydropteroate synthase(Enzyme) wildtype]

2_Amino_4_hydroxy_6_hydroxymethyl_7,8_dihydropteridine_diphosphate + 4_Aminobenzoate -> Dihydropteroate + Diphosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 5 mM DTT, 100 µg/ml BSA
PH: 8.3
Temperature: 37
uniprot:O05701 [dihydropteroate synthase(Enzyme) wildtype]

4_Aminobenzoate + 2_Amino_4_hydroxy_6_hydroxymethyl_7,8_dihydropteridine_diphosphate -> Dihydropteroate + Diphosphate		 (( (( (( (V1 + (V2 * A)) ) * A) ) * B) ) / (( (( (( (Kia * Km_B) ) + (Km_B * A)) ) + (Km_A * B)) ) + (( (B * A) ) * (1 + (A / K1))))) buffer: *
PH: 7
Temperature: 36
uniprot:O04862 [dihydropteroate synthase(Enzyme) wildtype]

4_Aminobenzoate + 2_Amino_4_hydroxy_6_hydroxymethyl_7,8_dihydropteridine_diphosphate -> Diphosphate + Dihydropteroate		 (( (Vmax * A) ) / (Km + A)) buffer: 40 mM Tris, 20 mM beta-mercaptoethanol, 10 mM MgCl2
PH: 8
Temperature: 30
uniprot:O04862 [dihydropteroate synthase(Enzyme) wildtype]

2_Amino_4_hydroxy_6_hydroxymethyl_7,8_dihydropteridine_diphosphate + 4_Aminobenzoate -> Diphosphate + Dihydropteroate		 (( (Vmax * B) ) / (Km + B)) buffer: 40 mM Tris, 20 mM beta-mercaptoethanol, 10 mM MgCl2
PH: 8
Temperature: 30
uniprot:O04862 [dihydropteroate synthase(Enzyme) wildtype]

4_Aminobenzoate + 2_Amino_4_hydroxy_6_hydroxymethyl_7,8_dihydropteridine_diphosphate -> Diphosphate + Dihydropteroate		 (( (Vmax * B) ) / (Km + B)) buffer: 40 mM Tris, 20 mM beta-mercaptoethanol, 10 mM MgCl2
PH: 8
Temperature: 30
uniprot:O04862 [dihydropteroate synthase(Enzyme) wildtype]

2_Amino_4_hydroxy_6_hydroxymethyl_7,8_dihydropteridine_diphosphate + 4_Aminobenzoate -> Diphosphate + Dihydropteroate		 (( (Vmax * A) ) / (Km + A)) buffer: 40 mM Tris, 20 mM beta-mercaptoethanol, 10 mM MgCl2
PH: 8
Temperature: 30
uniprot:P05382 [dihydropteroate synthase(Enzyme) mutant]

4_Aminobenzoate + 2_Amino_4_hydroxy_6_hydroxymethyl_7,8_dihydropteridine_diphosphate -> Dihydropteroate + Diphosphate		 (( (( (kcat * A) ) * E) ) / (Km + A)) buffer: 160 mM Tris-HCl, 80 mM MnCl2, 4 mg/ml ascorbic acid (pH 6.0)
PH: 8
Temperature: 37
uniprot:P05382 [dihydropteroate synthase(Enzyme) mutant]

2_Amino_4_hydroxy_6_hydroxymethyl_7,8_dihydropteridine_diphosphate + 4_Aminobenzoate -> Diphosphate + Dihydropteroate		 (( (( (kcat * A) ) * E) ) / (Km + A)) buffer: 160 mM Tris-HCl, 40 mM MnCl2, 4 mg/ml ascorbic acid (pH 6.0)
PH: 8
Temperature: 37
uniprot:P05382 [dihydropteroate synthase(Enzyme) wildtype]

2_Amino_4_hydroxy_6_hydroxymethyl_7,8_dihydropteridine_diphosphate + 4_Aminobenzoate -> Diphosphate + Dihydropteroate		 (( (( (kcat * A) ) * E) ) / (Km + A)) buffer: 160 mM Tris-HCl, 40 mM MnCl2, 4 mg/ml ascorbic acid (pH 6.0)
PH: 8
Temperature: 37
uniprot:P05382 [dihydropteroate synthase(Enzyme) wildtype]

4_Aminobenzoyl_glutamate + 2_Amino_4_hydroxy_6_hydroxymethyl_7,8_dihydropteridine_diphosphate -> Dihydrofolate + Diphosphate		 (( (( (kcat * A) ) * E) ) / (Km + A)) buffer: 160 mM Tris-HCl, 40 mM MnCl2, 4 mg/ml ascorbic acid (pH 6.0)
PH: 8
Temperature: 37
uniprot:P05382 [dihydropteroate synthase(Enzyme) mutant]

2_Amino_4_hydroxy_6_hydroxymethyl_7,8_dihydropteridine_diphosphate + 4_Aminobenzoyl_glutamate -> Diphosphate + Dihydrofolate		 (( (( (kcat * A) ) * E) ) / (Km + A)) buffer: 160 mM Tris-HCl, 40 mM MnCl2, 4 mg/ml ascorbic acid (pH 6.0)
PH: 8
Temperature: 37
uniprot:P05382 [dihydropteroate synthase(Enzyme) mutant]

2_Amino_4_hydroxy_6_hydroxymethyl_7,8_dihydropteridine_diphosphate + 4_Aminobenzoyl_glutamate -> Diphosphate + Dihydrofolate		 (( (( (kcat * A) ) * E) ) / (Km + A)) buffer: 160 mM Tris-HCl, 80 mM MnCl2, 4 mg/ml ascorbic acid (pH 6.0)
PH: 8
Temperature: 37

Pathways

pathway id name
BioCyc:META_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:ECO_PWY-6612 superpathway of tetrahydrofolate biosynthesis
BioCyc:YEAST_FOLSYN-PWY superpathway of tetrahydrofolate biosynthesis and salvage
BioCyc:ARA_PWY-6612 superpathway of tetrahydrofolate biosynthesis
BioCyc:ARA_PWY-6147 6-hydroxymethyl-dihydropterin diphosphate biosynthesis I
BioCyc:MTBH37RV_PWY-6612 superpathway of tetrahydrofolate biosynthesis
BioCyc:HPY_PWY-6614 tetrahydrofolate biosynthesis
BioCyc:ECOL316407_PWY-6614 tetrahydrofolate biosynthesis
BioCyc:ECOL316407_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:META_PWY-6612 superpathway of tetrahydrofolate biosynthesis
BioCyc:YEAST_PWY-6147 6-hydroxymethyl-dihydropterin diphosphate biosynthesis I
BioCyc:ARA_PWY-3742 tetrahydrofolate biosynthesis II
BioCyc:MTBH37RV_PWY-6614 tetrahydrofolate biosynthesis
BioCyc:MTBH37RV_FOLSYN-PWY superpathway of tetrahydrofolate biosynthesis and salvage
BioCyc:BSUB_PWY-6614 tetrahydrofolate biosynthesis
BioCyc:BSUB_PWY-6612 superpathway of tetrahydrofolate biosynthesis
BioCyc:ECOL316407_PWY-6612 superpathway of tetrahydrofolate biosynthesis
BioCyc:META_PWY-7539 6-hydroxymethyl-dihydropterin diphosphate biosynthesis III (Chlamydia)
BioCyc:META_PWY-6614 tetrahydrofolate biosynthesis
BioCyc:ECO_PWY-6614 tetrahydrofolate biosynthesis
BioCyc:YEAST_PWY-6612 superpathway of tetrahydrofolate biosynthesis
BioCyc:META_FOLSYN-PWY superpathway of tetrahydrofolate biosynthesis and salvage
BioCyc:ECO_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:YEAST_PWY-6614 tetrahydrofolate biosynthesis
BioCyc:ARA_PWY-6614 tetrahydrofolate biosynthesis
BioCyc:BSUB_FOLSYN-PWY superpathway of tetrahydrofolate biosynthesis and salvage
PathBank:SMP0000925 Folate Biosynthesis
PlantCyc:ARA_PWY-3742 tetrahydrofolate biosynthesis II
PlantCyc:ARA_PWY-6147 6-hydroxymethyl-dihydropterin diphosphate biosynthesis I
PlantCyc:PLANT_PWY-6614 tetrahydrofolate biosynthesis
PlantCyc:PLANT_PWY-6147 6-hydroxymethyl-dihydropterin diphosphate biosynthesis I
PlantCyc:ARA_PWY-6612 superpathway of tetrahydrofolate biosynthesis
PlantCyc:PLANT_PWY-3742 tetrahydrofolate biosynthesis II
PlantCyc:ARA_PWY-6614 tetrahydrofolate biosynthesis
PlantCyc:PLANT_PWY-6612 superpathway of tetrahydrofolate biosynthesis
PathBank:SMP0012014 Folate Biosynthesis
PathBank:SMP0012013 Pterin Biosynthesis (Folate Precursor)
PathBank:SMP0002334 Tetrahydrofolate Biosynthesis
PathBank:SMP0121306 Folate Biosynthesis