EC: 2.4.2.9

uracil phosphoribosyltransferase (UMP:diphosphate phospho-alpha-D-ribosyltransferase)

enzyme lambda metabolic reaction experiment
- [Mg2+]

5_Phospho_alpha_D_ribose_1_diphosphate + Uracil -> Diphosphate + Uridine_5'_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Tris-Cl
PH: 8.5
Temperature: 30
- [Mg2+]

Uracil + 5_Phospho_alpha_D_ribose_1_diphosphate -> Uridine_5'_phosphate + Diphosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Tris-Cl
PH: 8.5
Temperature: 30
- [GTP]

Uracil + 5_Phospho_alpha_D_ribose_1_diphosphate -> Diphosphate + Uridine_5'_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 10 mM KH2PO4, MgCl2
PH: 7.8
Temperature: 37
- [GTP]

5_Phospho_alpha_D_ribose_1_diphosphate + Uracil -> Uridine_5'_phosphate + Diphosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 10 mM KH2PO4, MgCl2
PH: 7.8
Temperature: 37
uniprot:P39765 [uracil phosphoribosyltransferase(Enzyme) wildtype]

Diphosphate + UMP -> Uracil + 5_Phospho_alpha_D_ribose_1_diphosphate		 (( (( (Vmax * A) ) * B) ) / (( (( (Kmb * A) ) + (Kma * B)) ) + (A * B))) buffer: 50 mM serine, 2.68 µg/µl adenine phosphoribosyltransferase, 2.5 mM MgCl2, 0.1 mM [14C]adenine
PH: 8.7
Temperature: 37
uniprot:P39765 [uracil phosphoribosyltransferase(Enzyme) wildtype]

5_Phospho_alpha_D_ribose_1_diphosphate + Uracil -> Diphosphate + UMP		 (( (( (Vmax * A) ) * B) ) / (( (( (Kmb * A) ) + (Kma * B)) ) + (A * B))) buffer: Tris acetate
PH: 8.7
Temperature: 37
uniprot:P0A8F0 [GTP]

5_Phospho_alpha_D_ribose_1_diphosphate + Uracil -> Diphosphate + UMP		 (( (kcat * E) ) / (( (( (( (( (( (Kis * Km_A) ) / A) ) * B) ) + (Km_B / B)) ) + (Km_A / A)) ) + 1)) buffer: 20 mM Tris/HCl, 5 mM MgCl2
PH: 8.5
Temperature: 37
uniprot:P0A8F0 [GTP]

5_Phospho_alpha_D_ribose_1_diphosphate + Uracil -> Diphosphate + UMP		 (( (kcat * E) ) / (( (( (( (( (( (Kis * Km_A) ) / A) ) * B) ) + (Km_B / B)) ) + (Km_A / A)) ) + 1)) buffer: 20 mM Tris/HCl, 5 mM MgCl2
PH: 8.5
Temperature: 37
uniprot:P0A8F0 [GTP]

Uracil + 5_Phospho_alpha_D_ribose_1_diphosphate -> UMP + Diphosphate		 (( (Vmax * B) ) / (Km + B)) buffer: 20 mM Tris/HCl, 5 mM MgCl2
PH: 8.5
Temperature: 37
uniprot:P0A8F0 [GTP]

5_Phospho_alpha_D_ribose_1_diphosphate + Uracil -> UMP + Diphosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Tris/HCl, 5 mM MgCl2
PH: 8.5
Temperature: 37
uniprot:P0A8F0 [GTP]

5_Phospho_alpha_D_ribose_1_diphosphate + Uracil -> Diphosphate + UMP		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Tris/HCl, 5 mM MgCl2
PH: 8.5
Temperature: 37
uniprot:P0A8F0 [GTP]

5_Phospho_alpha_D_ribose_1_diphosphate + Uracil -> Diphosphate + UMP		 (( (Vmax * B) ) / (Km + B)) buffer: 20 mM Tris/HCl, 5 mM MgCl2
PH: 8.5
Temperature: 37
uniprot:Q26998 [Mg2+]

Uracil + 5_Phospho_alpha_D_ribose_1_diphosphate -> Diphosphate + UMP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris, 2 mM dithiothreitol
PH: 7.5
Temperature: 37
uniprot:Q26998 [Mg2+]

Uracil + 5_Phospho_alpha_D_ribose_1_diphosphate -> UMP + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris, 2 mM dithiothreitol
PH: 7.5
Temperature: 37

Pathways

pathway id name
BioCyc:META_PWY-7194 pyrimidine nucleobases salvage II
BioCyc:ECO_PWY-7208 superpathway of pyrimidine nucleobases salvage
BioCyc:YEAST_PWY0-163 salvage pathways of pyrimidine ribonucleotides
BioCyc:ARA_PWY-7196 superpathway of pyrimidine ribonucleosides salvage
BioCyc:AGRO_PWY-7194 pyrimidine nucleobases salvage II
BioCyc:SYNEL_PWY-7194 pyrimidine nucleobases salvage II
BioCyc:CAULONA1000_P1-PWY salvage pathways of purine and pyrimidine nucleotides
BioCyc:PDIF272563_PWY-7183 pyrimidine nucleobases salvage I
BioCyc:META_PWY-7183 pyrimidine nucleobases salvage I
BioCyc:META_PWY-7196 superpathway of pyrimidine ribonucleosides salvage
BioCyc:ECO_PWY-7183 pyrimidine nucleobases salvage I
BioCyc:ECO_PWY-7194 pyrimidine nucleobases salvage II
BioCyc:ECO_PWY-7196 superpathway of pyrimidine ribonucleosides salvage
BioCyc:ARA_PWYQT-4445 pyrimidine salvage pathway
BioCyc:ARA_PWY-7193 pyrimidine ribonucleosides salvage I
BioCyc:MTBH37RV_PWY-7208 superpathway of pyrimidine nucleobases salvage
BioCyc:MTBCDC1551_PWY-7208 superpathway of pyrimidine nucleobases salvage
BioCyc:MTBCDC1551_PWY-7183 pyrimidine nucleobases salvage I
BioCyc:BSUB_P1-PWY purine and pyrimidine metabolism
BioCyc:META_PWY-7208 superpathway of pyrimidine nucleobases salvage
BioCyc:VCHO_PWY-7208 superpathway of pyrimidine nucleobases salvage
BioCyc:VCHO_PWY-7194 pyrimidine nucleobases salvage II
BioCyc:CAULO_PWY-7208 superpathway of pyrimidine nucleobases salvage
BioCyc:CAULO_PWY-7183 pyrimidine nucleobases salvage I
BioCyc:ANTHRA_PWY-7208 superpathway of pyrimidine nucleobases salvage
BioCyc:AGRO_PWY-7183 pyrimidine nucleobases salvage I
BioCyc:ECOL316407_PWY0-163 salvage pathways of pyrimidine ribonucleotides
BioCyc:PDIF272563_PWY-7208 superpathway of pyrimidine nucleobases salvage
BioCyc:CORYNE_PWY-7183 pyrimidine nucleobases salvage I
BioCyc:CORYNE_PWY-7194 pyrimidine nucleobases salvage II
BioCyc:GCF_000013425_PWY-7183 pyrimidine nucleobases salvage I
BioCyc:GCF_000013425_PWY-7208 superpathway of pyrimidine nucleobases salvage
BioCyc:ECO_PWY0-163 salvage pathways of pyrimidine ribonucleotides
BioCyc:YEAST_PWY-7183 pyrimidine nucleobases salvage I
BioCyc:ARA_PWY-7183 pyrimidine nucleobases salvage I
BioCyc:ARA_PWY-7208 superpathway of pyrimidine nucleobases salvage
BioCyc:VCHO_PWY-7183 pyrimidine nucleobases salvage I
BioCyc:MTBH37RV_PWY-7183 pyrimidine nucleobases salvage I
BioCyc:ANTHRA_PWY-7183 pyrimidine nucleobases salvage I
BioCyc:AGRO_PWY-7208 superpathway of pyrimidine nucleobases salvage
BioCyc:BSUB_PWY0-163 salvage pathways of pyrimidine ribonucleotides
BioCyc:SYNEL_PWY-7208 superpathway of pyrimidine nucleobases salvage
BioCyc:SYNEL_PWY-7183 pyrimidine nucleobases salvage I
BioCyc:CAULONA1000_PWY0-163 salvage pathways of pyrimidine ribonucleotides
BioCyc:CORYNE_PWY-7208 superpathway of pyrimidine nucleobases salvage
PlantCyc:ARA_PWYQT-4445 pyrimidine salvage pathway
PlantCyc:PLANT_PWY-7208 superpathway of pyrimidine nucleobases salvage
PlantCyc:ARA_PWY-7208 superpathway of pyrimidine nucleobases salvage
PlantCyc:PLANT_PWY-7183 pyrimidine nucleobases salvage I
PlantCyc:PLANT_PWY-7196 superpathway of pyrimidine ribonucleosides salvage
PlantCyc:ARA_PWY-7183 pyrimidine nucleobases salvage I
PlantCyc:PLANT_PWYQT-4445 pyrimidine salvage pathway
PlantCyc:ARA_PWY-7193 pyrimidine ribonucleosides salvage I
PlantCyc:ARA_PWY-7196 superpathway of pyrimidine ribonucleosides salvage