EC: 2.4.2.29

tRNA-guanosine34 preQ1 transglycosylase (tRNA-guanosine34:7-aminomethyl-7-deazaguanine tRNA-D-ribosyltransferase)

enzyme lambda metabolic reaction experiment
uniprot:P0A847 [tRNA-guanine transglycosylase(Enzyme) mutant htTGT(D143S)]

Guanine + tRNA_queuine -> Queuine + tRNA_guanine		 (( (( (E * kcat) ) * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) buffer: 100 mM HEPES, 20 mM MgCl2, 5 mM dithiothreitol
PH: 7.3
Temperature: 37
uniprot:P0A847 [tRNA-guanine transglycosylase(Enzyme) mutant htTGT(D143S)]

tRNA_queuine + Guanine -> Queuine + tRNA_guanine		 (( (( (E * kcat) ) * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) buffer: 100 mM HEPES, 20 mM MgCl2, 5 mM dithiothreitol
PH: 7.3
Temperature: 37
uniprot:P0A847 [tRNA-guanine transglycosylase(Enzyme) wildtype htTGT]

tRNA_guanine + Hypoxanthine -> Guanine + tRNA_hypoxanthine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM MgCl2, 5 mM dithiothreitol, 100 mM HEPES
PH: 7.3
Temperature: 37
uniprot:P0A847 [tRNA-guanine transglycosylase(Enzyme) mutant htTGT(D143N)]

tRNA_guanine + Hypoxanthine -> tRNA_hypoxanthine + Guanine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM MgCl2, 5 mM dithiothreitol, 100 mM HEPES
PH: 7.3
Temperature: 37
uniprot:P0A847 [tRNA-guanine transglycosylase(Enzyme) mutant htTGT(D143A)]

Hypoxanthine + tRNA_guanine -> Guanine + tRNA_hypoxanthine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM MgCl2, 5 mM dithiothreitol, 100 mM HEPES
PH: 7.3
Temperature: 37
uniprot:P0A847 [tRNA-guanine transglycosylase(Enzyme) mutant htTGT(D143N)]

Xanthine + tRNA_guanine -> Guanine + tRNA_xanthine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM MgCl2, 5 mM dithiothreitol, 100 mM HEPES
PH: 7.3
Temperature: 37
uniprot:P0A847 [tRNA-guanine transglycosylase(Enzyme) wildtype htTGT]

tRNA_guanine + Xanthine -> tRNA_xanthine + Guanine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM MgCl2, 5 mM dithiothreitol, 100 mM HEPES
PH: 7.3
Temperature: 37
uniprot:P0A847 [tRNA-guanine transglycosylase(Enzyme) mutant htTGT(D143A)]

tRNA_guanine + Xanthine -> tRNA_xanthine + Guanine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM MgCl2, 5 mM dithiothreitol, 100 mM HEPES
PH: 7.3
Temperature: 37

Pathways

pathway id name
BioCyc:ECOO157_PWY-6700 queuosine biosynthesis
BioCyc:ANTHRA_PWY-6700 queuosine biosynthesis
BioCyc:SYNEL_PWY-6700 queuosine biosynthesis
BioCyc:META_PWY-6700 queuosine biosynthesis
BioCyc:ECO_PWY-6700 queuosine biosynthesis
BioCyc:CAULO_PWY-6700 queuosine biosynthesis
BioCyc:GCF_000013425_PWY-6700 queuosine biosynthesis
BioCyc:HPY_PWY-6700 queuosine biosynthesis
BioCyc:AGRO_PWY-6700 queuosine biosynthesis
BioCyc:BSUB_PWY-6700 queuosine biosynthesis
BioCyc:ECOL316407_PWY-6700 queuosine biosynthesis
PathBank:SMP0001907 PreQ0 Metabolism
PathBank:SMP0122104 PreQ0 Metabolism