EC: 2.4.1.9

inulosucrase (sucrose:(2->1)-beta-D-fructan 1-beta-D-fructosyltransferase)

enzyme lambda metabolic reaction experiment
uniprot:Q74K42 [Ca2+]

Sucrose + H2O -> D_Fructose + D_Glucose
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 200 mM sodium acetate-sodium barbital
PH: 7
Temperature: 37
uniprot:Q74K42 [Ca2+]

(2,1_beta_D_Fructosyl)n + Sucrose -> Glucose + (2,1_beta_D_Fructosyl)n
(( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 200 mM sodium acetate-sodium barbital
PH: 7
Temperature: 55
uniprot:Q74K42 [Ca2+]

Sucrose + (2,1_beta_D_Fructosyl)n -> Glucose + (2,1_beta_D_Fructosyl)n
(( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 200 mM sodium acetate-sodium barbital
PH: 7
Temperature: 37
uniprot:Q8GP32 [Ca2+]

(2,1_beta_D_Fructosyl)n + Sucrose -> (2,1_beta_D_Fructosyl)n + Glucose
(( (( (kcat * E) ) * (S ^ n)) ) / (( (K50 ^ n) ) + (S ^ n))) buffer: 25 mM sodium acetate
PH: 5.4
Temperature: 20
uniprot:Q8GP32 [Ca2+]

(2,1_beta_D_Fructosyl)n + Sucrose -> Glucose + (2,1_beta_D_Fructosyl)n
(( (( (kcat * E) ) * (S ^ n)) ) / (( (K50 ^ n) ) + (S ^ n))) buffer: 25 mM sodium acetate
PH: 5.4
Temperature: 20
uniprot:Q8GP32 [Ca2+]

(2,1_beta_D_Fructosyl)n + Sucrose -> Glucose + (2,1_beta_D_Fructosyl)n
(( (( (kcat * E) ) * (S ^ n)) ) / (( (K50 ^ n) ) + (S ^ n))) buffer: 25 mM sodium acetate
PH: 5.4
Temperature: 50
uniprot:Q8GP32 [Ca2+]

(2,1_beta_D_Fructosyl)n + Sucrose -> Glucose + (2,1_beta_D_Fructosyl)n
(( (( (kcat * E) ) * (S ^ n)) ) / (( (K50 ^ n) ) + (S ^ n))) buffer: 25 mM sodium acetate
PH: 5.4
Temperature: 50

Pathways

pathway id name