EC: 2.4.1.187

N-acetylglucosaminyldiphosphoundecaprenol N-acetyl-beta-D-mannosaminyltransferase (UDP-N-acetyl-alpha-D-mannosamine:N-acetyl-alpha-D-glucosaminyldiphospho-ditrans,octacis-undecaprenol 4-beta-N-acetylmannosaminyltransferase (configuration-inverting))

enzyme lambda metabolic reaction experiment
uniprot:P27620 [N-acetylglucosaminyldiphosphoundecaprenol N-acetyl-beta-D-mannosaminyltransferase(Enzyme) wildtype]

UDP_N_acetyl_D_mannosamine + N_Acetyl_D_glucosaminyldiphosphotetradecan -> N_Acetyl_beta_D_mannosaminyl_1,4_N_acetyl_D_glucosaminyldiphosphotetradecan + UDP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris, 250 mM NaCl
PH: 7.8
Temperature: 22
uniprot:P27620 [N-acetylglucosaminyldiphosphoundecaprenol N-acetyl-beta-D-mannosaminyltransferase(Enzyme) wildtype]

N_Acetyl_D_glucosaminyldiphospho_(6Z,10Z,14Z)_2,6,10,14_tetramethyl_2,6,10,14_heptadecatetraene + UDP_N_acetyl_D_mannosamine -> N_Acetyl_beta_D_mannosaminyl_1,4_N_acetyl_D_glucosaminyldiphospho_(6Z,10Z,14Z)_2,6,10,14_tetramethyl_2,6,10,14_heptadecatetraene + UDP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris, 250 mM NaCl, 10% DMSO
PH: 7.8
Temperature: 22
uniprot:P27620 [N-acetylglucosaminyldiphosphoundecaprenol N-acetyl-beta-D-mannosaminyltransferase(Enzyme) wildtype]

UDP_N_acetyl_D_mannosamine + N_Acetyl_D_glucosaminyldiphospho_(6Z,10Z)_2,6,10_trimethyl_2,6,10_tridecatriene -> UDP + N_Acetyl_beta_D_mannosaminyl_1,4_N_acetyl_D_glucosaminyldiphospho_(6Z,10Z)_2,6,10_trimethyl_2,6,10_tridecatriene		 (( (( (( (kcat * E) ) * A) ) * B) ) / (( (( (( (Kia * Kb) ) + (Kb * A)) ) + (Ka * B)) ) + (A * B))) buffer: 50 mM Tris, 250 mM NaCl, 10% DMSO
PH: 7.8
Temperature: 22
uniprot:P27620 [N-acetylglucosaminyldiphosphoundecaprenol N-acetyl-beta-D-mannosaminyltransferase(Enzyme) wildtype]

N_Acetyl_D_glucosaminyldiphospho_(6Z,10Z)_2,6,10_trimethyl_2,6,10_tridecatriene + UDP_N_acetyl_D_mannosamine -> N_Acetyl_beta_D_mannosaminyl_1,4_N_acetyl_D_glucosaminyldiphospho_(6Z,10Z)_2,6,10_trimethyl_2,6,10_tridecatriene + UDP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris, 250 mM NaCl, 10% DMSO
PH: 7.8
Temperature: 22
uniprot:P27620 [N-acetylglucosaminyldiphosphoundecaprenol N-acetyl-beta-D-mannosaminyltransferase(Enzyme) wildtype]

UDP_N_acetyl_D_mannosamine + N_Acetyl_D_glucosaminyldiphospho_(6Z)_2,6_dimethyl_2,6_nonadiene -> UDP + N_Acetyl_beta_D_mannosaminyl_1,4_N_acetyl_D_glucosaminyldiphospho_(6Z)_2,6_dimethyl_2,6_nonadiene		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris, 250 mM NaCl
PH: 7.8
Temperature: 22
uniprot:P27620 [N-acetylglucosaminyldiphosphoundecaprenol N-acetyl-beta-D-mannosaminyltransferase(Enzyme) wildtype]

UDP_N_acetyl_D_mannosamine + N_Acetyl_D_glucosaminyldiphosphotetradecan -> N_Acetyl_beta_D_mannosaminyl_1,4_N_acetyl_D_glucosaminyldiphosphotetradecan + UDP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris, 250 mM NaCl
PH: 7.8
Temperature: 22

Pathways

pathway id name
BioCyc:META_PWY-7819 poly(3-O-β-D-glucopyranosyl-N-acetylgalactosamine 1-phosphate) wall teichoic acid biosynthesis