- [UDP-D-glucose]
Sucrose + UDP -> D_Fructose + UDP_D_glucose |
(( (Vmax * S) ) / (S + (Km * (1 + (I / Ki))))) |
|
buffer: 100 mM Tris/HCl, 2 mM MgCl2, 2 mM NAD+, 1 mM ATP, 4 U/ml Hexokinase, 4 U/ml PGI, 4 U/ml G6PDH
PH: 7
Temperature: 36 |
- [sucrose synthase(Enzyme) wildtype]
D_Fructose + UDP_D_glucose -> Sucrose + UDP |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 100 mM Tris/HCl, 15 mM MgCl2, 0.2 mM NADH, 1 mM phosphoenolpyruvate, 4 U/ml pyruvate kinase, 4 U/ml lactate dehydrogenase
PH: 7.3
Temperature: 36 |
- [sucrose synthase(Enzyme) wildtype]
D_Fructose + UDP_D_glucose -> UDP + Sucrose |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 100 mM Tris/HCl, 15 mM MgCl2, 0.2 mM NADH, 1 mM phosphoenolpyruvate, 4 U/ml pyruvate kinase, 4 U/ml lactate dehydrogenase
PH: 7.3
Temperature: 36 |
- [sucrose synthase(Enzyme) wildtype]
Sucrose + UDP -> UDP_D_glucose + D_Fructose |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 100 mM Tris/HCl, 2 mM MgCl2, 2 mM NAD+, 1 mM pyrophosphate, 4 U/ml UDPGlcPP, 4 U/ml PGM, 4 U/ml G6PDH
PH: 7
Temperature: 36 |
- [sucrose synthase(Enzyme) wildtype]
Sucrose + UDP -> UDP_D_glucose + D_Fructose |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 100 mM Tris/HCl, 2 mM MgCl2, 2 mM NAD+, 1 mM pyrophosphate, 4 U/ml UDPGlcPP, 4 U/ml PGM, 4 U/ml G6PDH
PH: 7
Temperature: 36 |
uniprot:P10691 [sucrose synthase(Enzyme) wildtype]
D_Fructose + UDP_D_glucose -> UDP + Sucrose |
(( (Vmax * A) ) / (( (Km + A) ) + (( (A ^ 2) ) / Ki))) |
|
buffer: 200 mM HEPES
PH: 8
Temperature: 30 |
uniprot:P10691 [sucrose synthase(Enzyme) wildtype]
D_Fructose + UDP_D_glucose -> UDP + Sucrose |
(( (Vmax * B) ) / (( (Km + B) ) + (( (B ^ 2) ) / Ki))) |
|
buffer: 200 mM HEPES
PH: 8
Temperature: 30 |
uniprot:P10691 [sucrose synthase(Enzyme) wildtype]
UDP_D_glucose + L_Sorbose -> alpha_D_Glucopyranosyl_alpha_L_sorbofuranoside + UDP |
(( (Vmax * B) ) / (( (Km + B) ) + (( (B ^ 2) ) / Ki))) |
|
buffer: 200 mM HEPES, 1 U/ml alkaline phosphatase
PH: 8
Temperature: 30 |
uniprot:P10691 [sucrose synthase(Enzyme) wildtype]
UDP_D_glucose + 1_Deoxy_1_fluoro_beta_D_fructose -> 1'_Deoxy_1'_fluoro_beta_D_fructofuranosyl_alpha_D_glucopyranoside + UDP |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 200 mM HEPES
PH: 8
Temperature: 30 |
uniprot:P10691 [sucrose synthase(Enzyme) wildtype]
UDP_D_glucose + D_Xylulose -> UDP + alpha_D_Glucopyranosyl_alpha_D_xylulofuranoside |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 200 mM HEPES, 1 U/ml alkaline phosphatase
PH: 8
Temperature: 30 |
uniprot:P10691 [sucrose synthase(Enzyme) wildtype]
UDP_D_glucose + D_Lyxose -> alpha_D_Glucopyranosyl_alpha_D_lyxopyranoside + UDP |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 200 mM HEPES, 1 U/ml alkaline phosphatase
PH: 8
Temperature: 30 |
uniprot:P10691 [sucrose synthase(Enzyme) wildtype]
UDP_N_acetyl_D_glucosamine + D_Fructose -> UDP + 2_Acetamido_2_deoxy_alpha_D_glucopyranosyl_beta_D_fructofuranoside |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 200 mM HEPES, 1 U/ml alkaline phosphatase
PH: 8
Temperature: 30 |
uniprot:P10691 [sucrose synthase(Enzyme) wildtype]
UDP_D_galactose + D_Fructose -> 1_alpha_D_Galactopyranosyl_2_beta_D_fructofuranoside + UDP |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 200 mM HEPES, 1 U/ml alkaline phosphatase
PH: 8
Temperature: 30 |
uniprot:Q9LKR0 [sucrose synthase(Enzyme) wildtype]
D_Fructose + UDP_D_glucose -> UDP + Sucrose |
(( (( (vol * (0 - Vmax8f)) ) * (( (Suc * UDP) ) - (( (( (Fru * 0.8231) ) * HexP) ) / Keq8))) ) / (( (( (( (( (Suc * UDP) ) * (1 + (Fru / Ki8Fru))) ) + (Km8Suc * (UDP + Ki8UDP))) ) + (Km8UDP * Suc)) ) + (( (Vmax8f / (Vmax8r * Keq8)) ) * (( (( (Km8UDPGlc * Fru) ) * (1 + (UDP / Ki8UDP))) ) + (( (0.8231 * HexP) ) * (( (Km8Fru * (1 + (( (Km8UDP * Suc) ) / (Ki8UDP * Km8Suc)))) ) + (Fru * (1 + (Suc / Ki8Suc))))))))) |
|
buffer: *
PH: 7
Temperature: 36 |
- [sucrose synthase(Enzyme) wildtype]
Sucrose + ADP -> ADP_alpha_D_glucoside + D_Fructose |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 200 mM Tris, 10 mM EDTA, 100 mM NaF
PH: 7.4
Temperature: 37 |
- [sucrose synthase(Enzyme) wildtype]
Sucrose + UDP -> D_Fructose + UDPglucose |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 200 mM Tris, 10 mM EDTA, 100 mM NaF
PH: 7.4
Temperature: 37 |
uniprot:P10691 [sucrose synthase(Enzyme) wildtype]
Sucrose + UDP -> D_Fructose + UDPglucose |
(( (Vmax * A) ) / (( (Km + A) ) + (( (A ^ 2) ) / Ki))) |
|
buffer: 50 mM HEPES
PH: 7.6
Temperature: 30 |
uniprot:P10691 [sucrose synthase(Enzyme) wildtype]
UDP + Sucrose -> D_Fructose + UDPglucose |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 50 mM HEPES
PH: 7.6
Temperature: 30 |
uniprot:Q9FRX3 [sucrose synthase(Enzyme) wildtype SS I]
D_Fructose + UDPglucose -> UDP + Sucrose |
(( (Vmax * A) ) / (Km_A + A)) |
|
buffer: 20 mM HEPES-KOH, 5 mM MgCl2, 4 mM Phosphoenolpyruvate, 0.15 mM NADH, 20 mM KCl, pyruvate kinase, lactate dehydrogenase
PH: 8
Temperature: 30 |
uniprot:Q9FRX3 [sucrose synthase(Enzyme) wildtype SS I]
D_Fructose + UDPglucose -> UDP + Sucrose |
(( (Vmax * B) ) / (Km_B + B)) |
|
buffer: 20 mM HEPES-KOH, 5 mM MgCl2, 4 mM Phosphoenolpyruvate, 0.15 mM NADH, 20 mM KCl, pyruvate kinase, lactate dehydrogenase
PH: 8
Temperature: 30 |
uniprot:P10691 [sucrose synthase(Enzyme) wildtype]
D_Fructose + UDPglucose -> UDP + Sucrose |
(( (Vmax * B) ) / (( (Km + B) ) + (( (B ^ 2) ) / Ki))) |
|
buffer: 50 mM HEPES
PH: 8
Temperature: 30 |
uniprot:P10691 [sucrose synthase(Enzyme) wildtype]
UDPglucose + D_Fructose -> UDP + Sucrose |
(( (Vmax * A) ) / (( (Km + A) ) + (( (A ^ 2) ) / Ki))) |
|
buffer: 50 mM HEPES
PH: 8
Temperature: 30 |
uniprot:P10691 [sucrose synthase(Enzyme) wildtype]
dTDP + Sucrose -> dTDPglucose + D_Fructose |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 50 mM Tris
PH: 7.2
Temperature: 30 |
uniprot:P10691 [sucrose synthase(Enzyme) wildtype]
D_Fructose + UDP_N_acetyl_D_glucosamine -> UDP + 2_Acetamido_2_deoxy_alpha_D_glucopyranosyl_beta_D_fructofuranoside |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 50 mM HEPES
PH: 8
Temperature: 30 |
uniprot:Q9FRX3 [sucrose synthase(Enzyme) wildtype SS II]
ADP_alpha_D_glucoside + D_Fructose -> ADP + Sucrose |
(( (Vmax * B) ) / (Km_B + B)) |
|
buffer: 20 mM HEPES-KOH, 5 mM MgCl2, 4 mM Phosphoenolpyruvate, 0.15 mM NADH, 20 mM KCl, pyruvate kinase, lactate dehydrogenase
PH: 8
Temperature: 30 |
uniprot:Q9FRX3 [sucrose synthase(Enzyme) wildtype SS I]
ADP_alpha_D_glucoside + D_Fructose -> ADP + Sucrose |
(( (Vmax * B) ) / (Km_B + B)) |
|
buffer: 20 mM HEPES-KOH, 5 mM MgCl2, 4 mM Phosphoenolpyruvate, 0.15 mM NADH, 20 mM KCl, pyruvate kinase, lactate dehydrogenase
PH: 8
Temperature: 30 |
uniprot:Q9FRX3 [sucrose synthase(Enzyme) wildtype SS I]
ADP + Sucrose -> ADP_alpha_D_glucoside + D_Fructose |
(( (Vmax * B) ) / (Km_B + B)) |
|
buffer: 30 mM HEPES-KOH
PH: 7
Temperature: 30 |
uniprot:Q9FRX3 [sucrose synthase(Enzyme) wildtype SS II]
D_Fructose + UDPglucose -> UDP + Sucrose |
(( (Vmax * A) ) / (Km_A + A)) |
|
buffer: 20 mM HEPES-KOH, 5 mM MgCl2, 4 mM Phosphoenolpyruvate, 0.15 mM NADH, 20 mM KCl, pyruvate kinase, lactate dehydrogenase
PH: 8
Temperature: 30 |
uniprot:Q9FRX3 [sucrose synthase(Enzyme) wildtype SS II]
UDPglucose + D_Fructose -> UDP + Sucrose |
(( (Vmax * B) ) / (Km_B + B)) |
|
buffer: 20 mM HEPES-KOH, 5 mM MgCl2, 4 mM Phosphoenolpyruvate, 0.15 mM NADH, 20 mM KCl, pyruvate kinase, lactate dehydrogenase
PH: 8
Temperature: 30 |
uniprot:Q9FRX3 [sucrose synthase(Enzyme) wildtype SS I]
UDP + Sucrose -> UDPglucose + D_Fructose |
(( (Vmax * A) ) / (Km_A + A)) |
|
buffer: 30 mM HEPES-KOH
PH: 7
Temperature: 30 |
uniprot:Q9FRX3 [sucrose synthase(Enzyme) wildtype SS I]
Sucrose + UDP -> UDPglucose + D_Fructose |
(( (Vmax * B) ) / (Km_B + B)) |
|
buffer: 30 mM HEPES-KOH
PH: 7
Temperature: 30 |
uniprot:Q9FRX3 [sucrose synthase(Enzyme) wildtype SS II]
UDP + Sucrose -> UDPglucose + D_Fructose |
(( (Vmax * B) ) / (Km_B + B)) |
|
buffer: 30 mM HEPES-KOH
PH: 7
Temperature: 30 |
uniprot:Q9FRX3 [sucrose synthase(Enzyme) wildtype SS II]
Sucrose + UDP -> D_Fructose + UDPglucose |
(( (Vmax * A) ) / (Km_A + A)) |
|
buffer: 30 mM HEPES-KOH
PH: 7
Temperature: 30 |
uniprot:Q9FRX3 [sucrose synthase(Enzyme) wildtype SS II]
ADP + Sucrose -> D_Fructose + ADP_alpha_D_glucoside |
(( (Vmax * B) ) / (Km_B + B)) |
|
buffer: 30 mM HEPES-KOH
PH: 7
Temperature: 30 |
uniprot:Q7Y078 [sucrose synthase(Enzyme) wildtype SS4]
ADP + Sucrose -> D_Fructose + ADP_alpha_D_glucoside |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 50 mM HEPES, 1 mM EDTA, 20 % polyethylene glycol, 1 mM MgCl2, 15 mM KCl
PH: 7
Temperature: 37 |
uniprot:P31923 [sucrose synthase(Enzyme) wildtype SS2]
ADP + Sucrose -> D_Fructose + ADP_alpha_D_glucoside |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 50 mM HEPES, 1 mM EDTA, 20 % polyethylene glycol, 1 mM MgCl2, 15 mM KCl
PH: 7
Temperature: 37 |
uniprot:P31923 [sucrose synthase(Enzyme) wildtype SS2]
Sucrose + ADP -> ADP_alpha_D_glucoside + D_Fructose |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 50 mM HEPES, 1 mM EDTA, 20 % polyethylene glycol, 1 mM MgCl2, 15 mM KCl
PH: 7
Temperature: 37 |
uniprot:P31923 [sucrose synthase(Enzyme) wildtype SS2]
Sucrose + UDP -> UDP_D_glucose + D_Fructose |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 50 mM HEPES, 1 mM EDTA, 20 % polyethylene glycol, 1 mM MgCl2, 15 mM KCl
PH: 7
Temperature: 37 |
uniprot:P31923 [sucrose synthase(Enzyme) wildtype SS2]
UDP + Sucrose -> UDP_D_glucose + D_Fructose |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 50 mM HEPES, 1 mM EDTA, 20 % polyethylene glycol, 1 mM MgCl2, 15 mM KCl
PH: 7
Temperature: 37 |
uniprot:Q7Y078 [sucrose synthase(Enzyme) wildtype SS4]
ADP + Sucrose -> D_Fructose + ADP_alpha_D_glucoside |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 50 mM HEPES, 1 mM EDTA, 20 % polyethylene glycol, 1 mM MgCl2, 15 mM KCl
PH: 7
Temperature: 37 |
uniprot:Q7Y078 [sucrose synthase(Enzyme) wildtype SS4]
UDP + Sucrose -> UDP_D_glucose + D_Fructose |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 50 mM HEPES, 1 mM EDTA, 20 % polyethylene glycol, 1 mM MgCl2, 15 mM KCl
PH: 7
Temperature: 37 |
uniprot:Q7Y078 [sucrose synthase(Enzyme) wildtype SS4]
UDP + Sucrose -> UDP_D_glucose + D_Fructose |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 50 mM HEPES, 1 mM EDTA, 20 % polyethylene glycol, 1 mM MgCl2, 15 mM KCl
PH: 7
Temperature: 37 |
uniprot:Q01390 [sucrose synthase(Enzyme) wildtype]
Sucrose + UDP -> D_Fructose + UDPglucose |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.05 M Sodium cacodylate
PH: 7.5
Temperature: 25 |
- [sucrose synthase(Enzyme) wildtype isoform A]
UDP + Sucrose -> UDP_D_glucose + D_Fructose |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 100 mM Tris-HCl, 2 mM MgCl2, 2 mM NAD+, 1 mM pyrophosphate, 4 U/ml UDP-glucose pyrophosphorylase, 4 U/ml phosphoglucomutase, 4 U/ml Leuconostoc glucose-6-P dehydrogenase
PH: 7
Temperature: 36 |
- [sucrose synthase(Enzyme) wildtype isoform A]
D_Fructose + UDP_D_glucose -> UDP + Sucrose |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 100 mM Tris-HCl
PH: 7
Temperature: 36 |
- [sucrose synthase(Enzyme) wildtype isoform A]
UDP_D_glucose + D_Fructose -> Sucrose + UDP |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 100 mM Tris-HCl
PH: 7
Temperature: 36 |
- [sucrose synthase(Enzyme) wildtype isoform B]
UDP_D_glucose + D_Fructose -> Sucrose + UDP |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 100 mM Tris-HCl
PH: 7
Temperature: 36 |
- [sucrose synthase(Enzyme) wildtype isoform A]
UDP + Sucrose -> UDP_D_glucose + D_Fructose |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 100 mM Tris-HCl, 2 mM MgCl2, 2 mM NAD+, 1 mM pyrophosphate, 4 U/ml UDP-glucose pyrophosphorylase, 4 U/ml phosphoglucomutase, 4 U/ml Leuconostoc glucose-6-P dehydrogenase
PH: 7
Temperature: 36 |
uniprot:P31924 [sucrose synthase(Enzyme) wildtype]
D_Fructose + UDPglucose -> Sucrose + UDP |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 143 mM Tris-acetate
PH: 8
Temperature: 37 |
- [sucrose synthase(Enzyme) wildtype isoform B]
UDP + Sucrose -> D_Fructose + UDP_D_glucose |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 100 mM Tris-HCl, 2 mM MgCl2, 2 mM NAD+, 1 mM pyrophosphate, 4 U/ml UDP-glucose pyrophosphorylase, 4 U/ml phosphoglucomutase, 4 U/ml Leuconostoc glucose-6-P dehydrogenase
PH: 7
Temperature: 36 |
- [sucrose synthase(Enzyme) wildtype isoform C]
Sucrose + UDP -> UDP_D_glucose + D_Fructose |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 100 mM Tris-HCl, 2 mM MgCl2, 2 mM NAD+, 1 mM pyrophosphate, 4 U/ml UDP-glucose pyrophosphorylase, 4 U/ml phosphoglucomutase, 4 U/ml Leuconostoc glucose-6-P dehydrogenase
PH: 7
Temperature: 36 |
- [sucrose synthase(Enzyme) wildtype isoform C]
UDP_D_glucose + D_Fructose -> UDP + Sucrose |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 100 mM Tris-HCl
PH: 7
Temperature: 36 |
- [sucrose synthase(Enzyme) wildtype isoform B]
D_Fructose + UDP_D_glucose -> Sucrose + UDP |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 100 mM Tris-HCl
PH: 7
Temperature: 36 |
- [sucrose synthase(Enzyme) wildtype isoform C]
UDP_D_glucose + D_Fructose -> UDP + Sucrose |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 100 mM Tris-HCl
PH: 7
Temperature: 36 |
- [sucrose synthase(Enzyme) wildtype isoform C]
Sucrose + UDP -> UDP_D_glucose + D_Fructose |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 100 mM Tris-HCl, 2 mM MgCl2, 2 mM NAD+, 1 mM pyrophosphate, 4 U/ml UDP-glucose pyrophosphorylase, 4 U/ml phosphoglucomutase, 4 U/ml Leuconostoc glucose-6-P dehydrogenase
PH: 7
Temperature: 36 |
- [sucrose synthase(Enzyme) wildtype isoform B]
UDP + Sucrose -> D_Fructose + UDP_D_glucose |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 100 mM Tris-HCl, 2 mM MgCl2, 2 mM NAD+, 1 mM pyrophosphate, 4 U/ml UDP-glucose pyrophosphorylase, 4 U/ml phosphoglucomutase, 4 U/ml Leuconostoc glucose-6-P dehydrogenase
PH: 7
Temperature: 36 |
uniprot:P31924 [sucrose synthase(Enzyme) wildtype]
Sucrose + ADP -> D_Fructose + ADP_alpha_D_glucoside |
(( (( (E * kcat) ) * (A ^ n)) ) / (( (S_half ^ n) ) + (A ^ n))) |
|
buffer: 100 mM Tris-acetate
PH: 6
Temperature: 37 |
uniprot:P31924 [sucrose synthase(Enzyme) wildtype]
Sucrose + ADP -> D_Fructose + ADP_alpha_D_glucoside |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 100 mM Tris-acetate
PH: 6
Temperature: 37 |
uniprot:P31924 [sucrose synthase(Enzyme) wildtype]
ADP_alpha_D_glucoside + D_Fructose -> Sucrose + ADP |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 143 mM Tris-acetate
PH: 8
Temperature: 37 |
uniprot:P31924 [sucrose synthase(Enzyme) wildtype]
ADP_alpha_D_glucoside + D_Fructose -> Sucrose + ADP |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 143 mM Tris-acetate
PH: 8
Temperature: 37 |
uniprot:P31924 [sucrose synthase(Enzyme) wildtype]
UDP + Sucrose -> D_Fructose + UDPglucose |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 100 mM Tris-acetate
PH: 6
Temperature: 37 |
uniprot:P31924 [sucrose synthase(Enzyme) wildtype]
Sucrose + UDP -> UDPglucose + D_Fructose |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 100 mM Tris-acetate
PH: 6
Temperature: 37 |
uniprot:P31924 [sucrose synthase(Enzyme) wildtype]
D_Fructose + UDPglucose -> Sucrose + UDP |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 143 mM Tris-acetate
PH: 8
Temperature: 37 |
uniprot:Q01390 [sucrose synthase(Enzyme) wildtype]
Sucrose + ADP -> D_Fructose + ADP_alpha_D_glucoside |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.05 M Sodium cacodylate
PH: 7.5
Temperature: 25 |
uniprot:Q01390 [sucrose synthase(Enzyme) wildtype]
ADP + Sucrose -> D_Fructose + ADP_alpha_D_glucoside |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.05 M Sodium cacodylate
PH: 7.5
Temperature: 25 |
uniprot:Q01390 [sucrose synthase(Enzyme) wildtype]
UDP + Sucrose -> D_Fructose + UDPglucose |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.05 M Sodium cacodylate
PH: 7.5
Temperature: 25 |
uniprot:Q01390 [sucrose synthase(Enzyme) wildtype]
Sucrose + GDP -> D_Fructose + GDPglucose |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.05 M Sodium cacodylate
PH: 7.5
Temperature: 25 |
uniprot:Q01390 [sucrose synthase(Enzyme) wildtype]
Sucrose + CDP -> CDPglucose + D_Fructose |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.05 M Sodium cacodylate
PH: 7.5
Temperature: 25 |
uniprot:Q01390 [sucrose synthase(Enzyme) wildtype]
TDP + Sucrose -> TDPglucose + D_Fructose |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.05 M Sodium cacodylate
PH: 7.5
Temperature: 25 |
- [sucrose synthase(Enzyme) wildtype]
Sucrose + ADP -> D_Fructose + ADP_alpha_D_glucoside |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM Hepes-KOH, 2 mM MgCl2, 1 mM EDTA, 15 mM KCl, 0.4 mM NAD, 1 mM ATP, 2 U/ml hexokinase, 2 U/ml glucose 6-phosphate dehydrogenase, 2 U/ml phosphoglucoseisomerase
PH: 7
Temperature: 25 |
- [sucrose synthase(Enzyme) wildtype]
ADP + Sucrose -> ADP_alpha_D_glucoside + D_Fructose |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Hepes-KOH, 2 mM MgCl2, 1 mM EDTA, 15 mM KCl, 0.4 mM NAD, 1 mM ATP, 2 U/ml hexokinase, 2 U/ml glucose 6-phosphate dehydrogenase, 2 U/ml phosphoglucoseisomerase
PH: 7
Temperature: 25 |
- [sucrose synthase(Enzyme) wildtype]
Sucrose + ADP -> D_Fructose + ADP_alpha_D_glucoside |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Hepes-KOH, 2 mM MgCl2, 1 mM EDTA, 15 mM KCl, 0.4 mM NAD, 1 mM ATP, 2 U/ml hexokinase, 2 U/ml glucose 6-phosphate dehydrogenase, 2 U/ml phosphoglucoseisomerase
PH: 7
Temperature: 25 |
- [sucrose synthase(Enzyme) wildtype]
UDP + Sucrose -> UDPglucose + D_Fructose |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM Hepes-KOH, 2 mM MgCl2, 1 mM EDTA, 15 mM KCl, 0.4 mM NAD, 1 mM ATP, 2 U/ml hexokinase, 2 U/ml glucose 6-phosphate dehydrogenase, 2 U/ml phosphoglucoseisomerase
PH: 7
Temperature: 25 |
uniprot:P31922 [sucrose synthase(Enzyme) wildtype]
Sucrose + UDP -> D_Fructose + UDPglucose |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Hepes, 0.03 U/ml UDPGlc-dehydrogenase, 0.5 mM NAD+
PH: 7.5
Temperature: 25 |
uniprot:P31922 [sucrose synthase(Enzyme) wildtype]
UDP + Sucrose -> UDPglucose + D_Fructose |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Hepes, 0.03 U/ml UDPGlc-dehydrogenase, 0.5 mM NAD+
PH: 7.5
Temperature: 25 |