EC: 2.3.1.74

chalcone synthase (malonyl-CoA:4-coumaroyl-CoA malonyltransferase (cyclizing))

enzyme lambda metabolic reaction experiment
- [naringenin-chalcone synthase(Enzyme) wildtype]

3 Malonyl_CoA + Cinnamoyl_CoA -> 4 Coenzyme_A + 2',4',6'_Trihydroxychalcone + 3 CO2		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 1 mM EDTA
PH: 7.5
Temperature: 30
- [naringenin-chalcone synthase(Enzyme) wildtype]

3 Malonyl_CoA + Cinnamoyl_diketide_NAC -> 2',4',6'_Trihydroxychalcone + 3 Coenzyme_A + 4 CO2 + N_Acetylcysteamine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 1 mM EDTA
PH: 7.5
Temperature: 30
- [naringenin-chalcone synthase(Enzyme) wildtype]

3 Malonyl_CoA + Cinnamoyl_NAC -> 2',4',6'_Trihydroxychalcone + 3 CO2 + N_Acetylcysteamine + 3 Coenzyme_A		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 1 mM EDTA
PH: 7.5
Temperature: 30
- [naringenin-chalcone synthase(Enzyme) wildtype]

3 Malonyl_CoA + 4_Coumaroyl_CoA -> 3 CO2 + 4 Coenzyme_A + Naringenin_chalcone		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 1.2 mM 2-mercaptoethanol
PH: 8
Temperature: 36
- [naringenin-chalcone synthase(Enzyme) wildtype]

3 Malonyl_CoA + 4_Coumaroyl_CoA -> 4 Coenzyme_A + 3 CO2 + Naringenin_chalcone		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 1.2 mM 2-mercaptoethanol
PH: 8
Temperature: 36
- [naringenin-chalcone synthase(Enzyme) wildtype CHS_H1 + CHIL2]

4_Coumaroyl_CoA + 3 Malonyl_CoA -> 4 Coenzyme_A + Naringenin_chalcone + 3 CO2		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 0.1 mg/ml BSA
PH: 7
Temperature: 30
uniprot:Q9FEY5 [naringenin-chalcone synthase(Enzyme) wildtype]

4_Coumaroyl_CoA + 3 Malonyl_CoA -> Naringenin_chalcone + 3 CO2 + 4 Coenzyme_A		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 0.1 mg/ml BSA
PH: 7
Temperature: 30
- [naringenin-chalcone synthase(Enzyme) wildtype CHS_H1 + CHIL2]

4_Coumaroyl_CoA + 3 Malonyl_CoA -> 3 CO2 + 4 Coenzyme_A + Naringenin_chalcone		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 0.1 mg/ml BSA
PH: 7
Temperature: 30
uniprot:Q9FEY5 [naringenin-chalcone synthase(Enzyme) wildtype]

4_Coumaroyl_CoA + 3 Malonyl_CoA -> 3 CO2 + Naringenin_chalcone + 4 Coenzyme_A		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 0.1 mg/ml BSA
PH: 7
Temperature: 30

Pathways

pathway id name
BioCyc:META_PWY-7857 adlupulone and adhumulone biosynthesis
BioCyc:META_PWY1F-FLAVSYN flavonoid biosynthesis
BioCyc:ARA_PWY-6787 flavonoid biosynthesis (in equisetum)
BioCyc:ARA_PWY1F-FLAVSYN flavonoid biosynthesis
BioCyc:META_PWY-5135 xanthohumol biosynthesis
BioCyc:META_PWY-5133 colupulone and cohumulone biosynthesis
BioCyc:META_PWY-5059 pinobanksin biosynthesis
BioCyc:META_PWY-5134 superpathway of bitter acids biosynthesis
BioCyc:META_PWY-5132 lupulone and humulone biosynthesis
BioCyc:META_PWY-5393 raspberry ketone biosynthesis
BioCyc:META_PWY-6316 aromatic polyketides biosynthesis
Plant Reactome:R-OSA-2744345 Metabolism and regulation
Plant Reactome:R-OSA-2744344 Secondary metabolism
Plant Reactome:R-OSA-1119513 Pinobanksin biosynthesis
Plant Reactome:R-OSA-1119630 Resveratrol biosynthesis
PlantCyc:ARA_PWY-6787 flavonoid biosynthesis (in equisetum)
PlantCyc:HOP_PWY1F-FLAVSYN flavonoid biosynthesis
PlantCyc:HOP_PWY-7857 adlupulone and adhumulone biosynthesis
PlantCyc:HOP_PWY-5134 superpathway of bitter acids biosynthesis
PlantCyc:HOP_PWY-5133 colupulone and cohumulone biosynthesis
PlantCyc:PLANT_PWY-5132 lupulone and humulone biosynthesis
PlantCyc:PLANT_PWY1F-FLAVSYN flavonoid biosynthesis
PlantCyc:HOP_PWY-84 resveratrol biosynthesis
PlantCyc:HOP_PWY-5132 lupulone and humulone biosynthesis
PlantCyc:PLANT_PWY-7857 adlupulone and adhumulone biosynthesis
PlantCyc:PLANT_PWY-5134 superpathway of bitter acids biosynthesis
PlantCyc:PLANT_PWY-5133 colupulone and cohumulone biosynthesis
PlantCyc:ARA_PWY1F-FLAVSYN flavonoid biosynthesis
PlantCyc:HOP_PWY-5135 xanthohumol biosynthesis
PlantCyc:HOP_PWY-6787 flavonoid biosynthesis (in equisetum)
PlantCyc:HOP_PWY-7897 flavonoid di-C-glucosylation
PlantCyc:PLANT_PWY-6316 aromatic polyketides biosynthesis
PlantCyc:PLANT_PWY-5135 xanthohumol biosynthesis
PathBank:SMP0012021 Flavonoid Biosynthesis
PathBank:SMP0012024 Flavanone Biosynthesis