EC: 2.3.1.4

glucosamine-phosphate N-acetyltransferase (acetyl-CoA:D-glucosamine-6-phosphate N-acetyltransferase)

enzyme lambda metabolic reaction experiment
uniprot:Q96EK6 [glucosamine-phosphate N-acetyltransferase(Enzyme) wildtype]

D_Glucosamine_6_phosphate + Acetyl_CoA -> N_Acetyl_D_glucosamine_6_phosphate + Coenzyme_A		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM Tris/HCl, 250 mM NaCl, 2 mM EDTA, 5% (v/v) Glycerol
PH: 7.5
Temperature: 22
uniprot:Q4WCU5 [glucosamine-phosphate N-acetyltransferase(Enzyme) mutant Y189R]

D_Glucosamine_6_phosphate + Acetyl_CoA -> Coenzyme_A + N_Acetyl_D_glucosamine_6_phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM Tris/HCl, 250 mM NaCl, 2 mM EDTA, 5% (v/v) Glycerol
PH: 7.5
Temperature: 22
uniprot:Q4WCU5 [glucosamine-phosphate N-acetyltransferase(Enzyme) mutant Y189R]

Acetyl_CoA + D_Glucosamine_6_phosphate -> N_Acetyl_D_glucosamine_6_phosphate + Coenzyme_A		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM Tris/HCl, 250 mM NaCl, 2 mM EDTA, 5% (v/v) Glycerol
PH: 7.5
Temperature: 22
uniprot:Q4WCU5 [glucosamine-phosphate N-acetyltransferase(Enzyme) wildtype]

D_Glucosamine_6_phosphate + Acetyl_CoA -> N_Acetyl_D_glucosamine_6_phosphate + Coenzyme_A		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM Tris/HCl, 250 mM NaCl, 2 mM EDTA, 5% (v/v) Glycerol
PH: 7.5
Temperature: 22
uniprot:Q96EK6 [glucosamine-phosphate N-acetyltransferase(Enzyme) wildtype]

D_Glucosamine_6_phosphate + Acetyl_CoA -> N_Acetyl_D_glucosamine_6_phosphate + Coenzyme_A		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM Tris/HCl, 250 mM NaCl, 2 mM EDTA, 5% (v/v) Glycerol
PH: 7.5
Temperature: 22
uniprot:Q4WCU5 [glucosamine-phosphate N-acetyltransferase(Enzyme) mutant G183E]

Acetyl_CoA + D_Glucosamine_6_phosphate -> Coenzyme_A + N_Acetyl_D_glucosamine_6_phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM Tris/HCl, 250 mM NaCl, 2 mM EDTA, 5% (v/v) Glycerol
PH: 7.5
Temperature: 22
uniprot:Q4WCU5 [glucosamine-phosphate N-acetyltransferase(Enzyme) mutant E185Y]

D_Glucosamine_6_phosphate + Acetyl_CoA -> N_Acetyl_D_glucosamine_6_phosphate + Coenzyme_A		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM Tris/HCl, 250 mM NaCl, 2 mM EDTA, 5% (v/v) Glycerol
PH: 7.5
Temperature: 22
uniprot:Q4WCU5 [glucosamine-phosphate N-acetyltransferase(Enzyme) mutant V125R]

D_Glucosamine_6_phosphate + Acetyl_CoA -> N_Acetyl_D_glucosamine_6_phosphate + Coenzyme_A		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM Tris/HCl, 250 mM NaCl, 2 mM EDTA, 5% (v/v) Glycerol
PH: 7.5
Temperature: 22
uniprot:Q4WCU5 [glucosamine-phosphate N-acetyltransferase(Enzyme) wildtype]

D_Glucosamine_6_phosphate + Acetyl_CoA -> N_Acetyl_D_glucosamine_6_phosphate + Coenzyme_A		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM Tris/HCl, 250 mM NaCl, 2 mM EDTA, 5% (v/v) Glycerol
PH: 7.5
Temperature: 22
uniprot:Q4WCU5 [glucosamine-phosphate N-acetyltransferase(Enzyme) mutant G183E]

D_Glucosamine_6_phosphate + Acetyl_CoA -> Coenzyme_A + N_Acetyl_D_glucosamine_6_phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM Tris/HCl, 250 mM NaCl, 2 mM EDTA, 5% (v/v) Glycerol
PH: 7.5
Temperature: 22
uniprot:Q4WCU5 [glucosamine-phosphate N-acetyltransferase(Enzyme) mutant V125R]

D_Glucosamine_6_phosphate + Acetyl_CoA -> N_Acetyl_D_glucosamine_6_phosphate + Coenzyme_A		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM Tris/HCl, 250 mM NaCl, 2 mM EDTA, 5% (v/v) Glycerol
PH: 7.5
Temperature: 22
uniprot:Q4WCU5 [glucosamine-phosphate N-acetyltransferase(Enzyme) mutant E185Y]

Acetyl_CoA + D_Glucosamine_6_phosphate -> Coenzyme_A + N_Acetyl_D_glucosamine_6_phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM Tris/HCl, 250 mM NaCl, 2 mM EDTA, 5% (v/v) Glycerol
PH: 7.5
Temperature: 22
uniprot:Q7SHA4 [glucosamine-phosphate N-acetyltransferase(Enzyme) wildtype]

Acetyl_CoA + D_Glucosamine_6_phosphate -> N_Acetyl_D_glucosamine_6_phosphate + Coenzyme_A		 (( (Vmax * A) ) / (Km + A)) buffer: 28.57 mM Phosphate
PH: 6.6
Temperature: 37
uniprot:Q7SHA4 [glucosamine-phosphate N-acetyltransferase(Enzyme) wildtype]

D_Glucosamine_6_phosphate + Acetyl_CoA -> Coenzyme_A + N_Acetyl_D_glucosamine_6_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 28.57 mM Phosphate
PH: 6.6
Temperature: 37
- [glucosamine-phosphate N-acetyltransferase(Enzyme) wildtype]

D_Glucosamine_6_phosphate + Acetyl_CoA -> N_Acetyl_D_glucosamine_6_phosphate + Coenzyme_A		 (( (Vmax * A) ) / (Km + A)) buffer: 0.3 M Tris-HCl
PH: 7.85
Temperature: 37
- [glucosamine-phosphate N-acetyltransferase(Enzyme) wildtype]

D_Glucosamine_6_phosphate + Acetyl_CoA -> N_Acetyl_D_glucosamine_6_phosphate + Coenzyme_A		 (( (Vmax * B) ) / (Km + B)) buffer: 0.3 M Tris-HCl
PH: 7.85
Temperature: 37

Pathways

pathway id name
BioCyc:YEAST_UDPNAGSYN-YEAST-PWY UDP-N-acetylglucosamine biosynthesis
BioCyc:YEAST_PWY-6981 chitin biosynthesis
BioCyc:HUMAN_UDPNACETYLGALSYN-PWY UDP-N-acetyl-D-glucosamine biosynthesis II
BioCyc:META_UDPNACETYLGALSYN-PWY UDP-N-acetyl-D-glucosamine biosynthesis II
BioCyc:MOUSE_UDPNACETYLGALSYN-PWY UDP-N-acetyl-D-glucosamine biosynthesis II
BioCyc:MOUSE_PWY-5514 UDP-N-acetyl-D-galactosamine biosynthesis II
BioCyc:HUMAN_PWY-5514 UDP-N-acetyl-D-galactosamine biosynthesis II
BioCyc:META_PWY-6981 chitin biosynthesis
BioCyc:YEAST_UDPNACETYLGALSYN-PWY UDP-N-acetyl-D-glucosamine biosynthesis II
Plant Reactome:R-OSA-9030769 Growth and developmental processes
Plant Reactome:R-OSA-9031670 Vegetative structure development
Plant Reactome:R-OSA-9640032 Root structure development
Plant Reactome:R-OSA-9030654 Primary root development
Reactome:R-DDI-597592 Post-translational protein modification
Reactome:R-DDI-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome:R-DME-597592 Post-translational protein modification
Reactome:R-DME-446219 Synthesis of substrates in N-glycan biosythesis
Reactome:R-HSA-392499 Metabolism of proteins
Reactome:R-HSA-597592 Post-translational protein modification
Reactome:R-MMU-446219 Synthesis of substrates in N-glycan biosythesis
Reactome:R-SCE-597592 Post-translational protein modification
Reactome:R-SCE-446219 Synthesis of substrates in N-glycan biosythesis
Reactome:R-SPO-446203 Asparagine N-linked glycosylation
Reactome:R-SPO-446210 Synthesis of UDP-N-acetyl-glucosamine
Reactome:R-DDI-392499 Metabolism of proteins
Reactome:R-DDI-446203 Asparagine N-linked glycosylation
Reactome:R-DDI-446210 Synthesis of UDP-N-acetyl-glucosamine
Reactome:R-DME-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome:R-HSA-446219 Synthesis of substrates in N-glycan biosythesis
Reactome:R-MMU-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome:R-SCE-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome:R-DDI-446219 Synthesis of substrates in N-glycan biosythesis
Reactome:R-DME-392499 Metabolism of proteins
Reactome:R-HSA-446203 Asparagine N-linked glycosylation
Reactome:R-HSA-446210 Synthesis of UDP-N-acetyl-glucosamine
Reactome:R-MMU-392499 Metabolism of proteins
Reactome:R-MMU-597592 Post-translational protein modification
Reactome:R-SPO-392499 Metabolism of proteins
Reactome:R-SPO-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome:R-DME-446203 Asparagine N-linked glycosylation
Reactome:R-DME-446210 Synthesis of UDP-N-acetyl-glucosamine
Reactome:R-HSA-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome:R-MMU-446203 Asparagine N-linked glycosylation
Reactome:R-MMU-446210 Synthesis of UDP-N-acetyl-glucosamine
Reactome:R-SCE-392499 Metabolism of proteins
Reactome:R-SCE-446203 Asparagine N-linked glycosylation
Reactome:R-SCE-446210 Synthesis of UDP-N-acetyl-glucosamine
Reactome:R-SPO-597592 Post-translational protein modification
Reactome:R-SPO-446219 Synthesis of substrates in N-glycan biosythesis
WikiPathways:WP5220 Metabolic reprogramming in pancreatic cancer
PathBank:SMP0120496 Hyperinsulinism-Hyperammonemia Syndrome
PathBank:SMP0120524 Salla Disease/Infantile Sialic Acid Storage Disease
PathBank:SMP0000339 Hyperinsulinism-Hyperammonemia Syndrome
PathBank:SMP0000240 Salla Disease/Infantile Sialic Acid Storage Disease
PathBank:SMP0002331 Amino Sugar and Nucleotide Sugar Metabolism
PathBank:SMP0087374 Amino Sugar Metabolism
PathBank:SMP0000045 Amino Sugar Metabolism
PathBank:SMP0000385 Homocarnosinosis
PathBank:SMP0000216 Sialuria or French Type Sialuria
PathBank:SMP0000136 2-Hydroxyglutric Aciduria (D and L Form)
PathBank:SMP0000567 Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0120445 4-Hydroxybutyric Aciduria/Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0120517 Sialuria or French Type Sialuria
PathBank:SMP0120545 Tay-Sachs Disease
PathBank:SMP0000390 Tay-Sachs Disease
PathBank:SMP0087428 Glutamate Metabolism
PathBank:SMP0063590 Glutamate Metabolism
PathBank:SMP0120438 2-Hydroxyglutric Aciduria (D and L Form)
PathBank:SMP0120494 Homocarnosinosis
PathBank:SMP0120599 G(M2)-Gangliosidosis: Variant B, Tay-Sachs Disease
PathBank:SMP0120631 Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0000072 Glutamate Metabolism
PathBank:SMP0000243 4-Hydroxybutyric Aciduria/Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0000534 G(M2)-Gangliosidosis: Variant B, Tay-Sachs Disease
PathBank:SMP0087371 Glutamate Metabolism
PathBank:SMP0094667 Chitin Biosynthesis
PathBank:SMP0063580 Amino Sugar Metabolism