EC: 2.2.1.6

acetolactate synthase (pyruvate:pyruvate acetaldehydetransferase (decarboxylating))

enzyme lambda metabolic reaction experiment
uniprot:P17597 [acetolactate synthase(Enzyme) mutant GH50(csr1-1)]

2 Pyruvate -> CO2 + 2_Acetolactate		 (( (Vmax * S) ) / (Km + S)) buffer: 130 mM Potassium phosphate, 20 mM MgCl2, 0.5 mM Thiamine pyrophosphate, 46 µM Flavine adenine dinucleotide
PH: 7.5
Temperature: 30
uniprot:P17597 [acetolactate synthase(Enzyme) mutant GM4.8(csr1-4)]

2 Pyruvate -> 2_Acetolactate + CO2		 (( (Vmax * S) ) / (Km + S)) buffer: 130 mM Potassium phosphate, 20 mM MgCl2, 0.5 mM Thiamine pyrophosphate, 46 µM Flavine adenine dinucleotide
PH: 7.5
Temperature: 30
uniprot:P17597 [acetolactate synthase(Enzyme) wildtype CSR1]

2 Pyruvate -> 2_Acetolactate + CO2		 (( (Vmax * S) ) / (Km + S)) buffer: 130 mM Potassium phosphate, 20 mM MgCl2, 0.5 mM Thiamine pyrophosphate, 46 µM Flavine adenine dinucleotide
PH: 7.5
Temperature: 30
uniprot:P17597 [acetolactate synthase(Enzyme) mutant Tzp5(csr1-3)]

2 Pyruvate -> CO2 + 2_Acetolactate		 (( (Vmax * S) ) / (Km + S)) buffer: 130 mM Potassium phosphate, 20 mM MgCl2, 0.5 mM Thiamine pyrophosphate, 46 µM Flavine adenine dinucleotide
PH: 7.5
Temperature: 30
uniprot:P17597 [acetolactate synthase(Enzyme) mutant GH90(csr1-2)]

2 Pyruvate -> CO2 + 2_Acetolactate		 (( (Vmax * S) ) / (Km + S)) buffer: 130 mM Potassium phosphate, 20 mM MgCl2, 0.5 mM Thiamine pyrophosphate, 46 µM Flavine adenine dinucleotide
PH: 7.5
Temperature: 30
- [2-Oxobutyrate]

Pyruvate -> (S)_2_Acetolactate + CO2		 (( (Va * A) ) / (A + (R * B))) buffer: 0.1 M potassium phosphate, 10 mM MgCl2, 0.1 mM Thiamin diphosphate, 75 µM FAD
PH: 7.6
Temperature: 37
- [Benzaldehyde]

Pyruvate -> (S)_2_Acetolactate + CO2		 (( (( (Va * Ki) ) * A) ) / (( (A + (f * B)) ) * (Ki + B))) buffer: 0.1 M Hepes/KOH, 5 mM MgCl2, 0.1 mM ThDP, 50 µM FAD, 60 mM KCl, 0.5 mM DTT, 10% Me2SO4 (v/v)
PH: 7
Temperature: 30
- [Benzaldehyde]

Pyruvate -> CO2 + (S)_2_Acetolactate		 (( (( (Va * Ki) ) * A) ) / (( (A + (f * B)) ) * (Ki + B))) buffer: 0.1 M Hepes/KOH, 5 mM MgCl2, 0.1 mM Thiamin diphosphate, 50 µM FAD, 60 mM KCl, 0.5 mM DTT, 10% Me2SO4 (v/v)
PH: 7
Temperature: 30
- [acetolactate synthase(Enzyme) wildtype AHAS II]

2_Oxobutyrate + Pyruvate -> CO2 + (S)_2_Aceto_2_hydroxybutanoate		 (( (( (Va * R) ) * B) ) / (A + (R * B))) buffer: 0.1 M potassium phosphate, 10 mM MgCl2, 0.1 mM Thiamin diphosphate, 75 µM FAD
PH: 7.6
Temperature: 37
- [Benzaldehyde]

Pyruvate -> (S)_2_Acetolactate + CO2		 (( (( (Va * Ki) ) * A) ) / (( (A + (f * B)) ) * (Ki + B))) buffer: 0.1 M Hepes/KOH, 5 mM MgCl2, 0.1 mM Thiamin diphosphate, 50 µM FAD, 60 mM KCl, 0.5 mM DTT, 10% Me2SO4 (v/v)
PH: 7
Temperature: 30
- [Benzaldehyde]

Pyruvate -> CO2 + (S)_2_Acetolactate		 (( (( (Va * Ki) ) * A) ) / (( (A + (f * B)) ) * (Ki + B))) buffer: 0.1 M Hepes/KOH, 5 mM MgCl2, 0.1 mM Thiamin diphosphate, 50 µM FAD, 60 mM KCl, 0.5 mM DTT, 10% Me2SO4 (v/v)
PH: 7
Temperature: 30
- [Benzaldehyde]

Pyruvate -> CO2 + (S)_2_Acetolactate		 (( (( (Va * Ki) ) * A) ) / (( (A + (f * B)) ) * (Ki + B))) buffer: 0.1 M Hepes/KOH, 5 mM MgCl2, 0.1 mM Thiamin diphosphate, 50 µM FAD, 60 mM KCl, 0.5 mM DTT, 10% Me2SO4 (v/v)
PH: 7
Temperature: 30
- [Benzaldehyde]

Pyruvate -> (S)_2_Acetolactate + CO2		 (( (( (Va * Ki) ) * A) ) / (( (A + (f * B)) ) * (Ki + B))) buffer: 0.1 M Hepes/KOH, 5 mM MgCl2, 0.1 mM Thiamin diphosphate, 50 µM FAD, 60 mM KCl, 0.5 mM DTT, 10% Me2SO4 (v/v)
PH: 7
Temperature: 30
- [Benzaldehyde]

Pyruvate -> CO2 + (S)_2_Acetolactate		 (( (( (Va * Ki) ) * A) ) / (( (A + (f * B)) ) * (Ki + B))) buffer: 0.1 M Hepes/KOH, 5 mM MgCl2, 0.1 mM Thiamin diphosphate, 50 µM FAD, 60 mM KCl, 0.5 mM DTT, 10% Me2SO4 (v/v)
PH: 7
Temperature: 30
- [Benzaldehyde]

Pyruvate -> CO2 + (S)_2_Acetolactate		 (( (( (Va * Ki) ) * A) ) / (( (A + (f * B)) ) * (Ki + B))) buffer: 0.1 M Hepes/KOH, 5 mM MgCl2, 0.1 mM Thiamin diphosphate, 50 µM FAD, 60 mM KCl, 0.5 mM DTT, 10% Me2SO4 (v/v)
PH: 7
Temperature: 30
- [Benzaldehyde]

Pyruvate -> (S)_2_Acetolactate + CO2		 (( (( (Va * Ki) ) * A) ) / (( (A + (f * B)) ) * (Ki + B))) buffer: 0.1 M Hepes/KOH, 5 mM MgCl2, 0.1 mM Thiamin diphosphate, 50 µM FAD, 60 mM KCl, 0.5 mM DTT, 10% Me2SO4 (v/v)
PH: 7
Temperature: 30
- [Benzaldehyde]

Pyruvate -> (S)_2_Acetolactate + CO2		 (( (( (Va * Ki) ) * A) ) / (( (A + (f * B)) ) * (Ki + B))) buffer: 0.1 M Hepes/KOH, 5 mM MgCl2, 0.1 mM Thiamin diphosphate, 50 µM FAD, 60 mM KCl, 0.5 mM DTT, 10% Me2SO4 (v/v)
PH: 7
Temperature: 30
- [Benzaldehyde]

Pyruvate -> CO2 + (S)_2_Acetolactate		 (( (( (Va * Ki) ) * A) ) / (( (A + (f * B)) ) * (Ki + B))) buffer: 0.1 M Hepes/KOH, 5 mM MgCl2, 0.1 mM Thiamin diphosphate, 50 µM FAD, 60 mM KCl, 0.5 mM DTT, 10% Me2SO4 (v/v)
PH: 7
Temperature: 30
- [acetolactate synthase(Enzyme) wildtype AHAS II]

Benzaldehyde + Pyruvate -> CO2 + R_Phenylacetylcarbinol		 (( (( (( (Vc * Ki) ) * f) ) * B) ) / (( (A + (f * B)) ) * (Ki + B))) buffer: 0.1 M Hepes/KOH, 5 mM MgCl2, 0.1 mM Thiamin diphosphate, 50 µM FAD, 60 mM KCl, 0.5 mM DTT, 10% Me2SO4 (v/v)
PH: 7
Temperature: 30
- [acetolactate synthase(Enzyme) mutant AHASII F109M]

Pyruvate + Benzaldehyde -> CO2 + R_Phenylacetylcarbinol		 (( (( (( (Vc * Ki) ) * f) ) * B) ) / (( (A + (f * B)) ) * (Ki + B))) buffer: 0.1 M Hepes/KOH, 5 mM MgCl2, 0.1 mM Thiamin diphosphate, 50 µM FAD, 60 mM KCl, 0.5 mM DTT, 10% Me2SO4 (v/v)
PH: 7
Temperature: 30
- [acetolactate synthase(Enzyme) mutant AHASII R276Q]

Pyruvate + Benzaldehyde -> R_Phenylacetylcarbinol + CO2		 (( (( (( (Vc * Ki) ) * f) ) * B) ) / (( (A + (f * B)) ) * (Ki + B))) buffer: 0.1 M Hepes/KOH, 5 mM MgCl2, 0.1 mM Thiamin diphosphate, 50 µM FAD, 60 mM KCl, 0.5 mM DTT, 10% Me2SO4 (v/v)
PH: 7
Temperature: 30
- [acetolactate synthase(Enzyme) mutant AHASII R276K]

Benzaldehyde + Pyruvate -> R_Phenylacetylcarbinol + CO2		 (( (( (( (Vc * Ki) ) * f) ) * B) ) / (( (A + (f * B)) ) * (Ki + B))) buffer: 0.1 M Hepes/KOH, 5 mM MgCl2, 0.1 mM Thiamin diphosphate, 50 µM FAD, 60 mM KCl, 0.5 mM DTT, 10% Me2SO4 (v/v)
PH: 7
Temperature: 30
- [acetolactate synthase(Enzyme) mutant AHASII R276F]

Pyruvate + Benzaldehyde -> R_Phenylacetylcarbinol + CO2		 (( (( (( (Vc * Ki) ) * f) ) * B) ) / (( (A + (f * B)) ) * (Ki + B))) buffer: 0.1 M Hepes/KOH, 5 mM MgCl2, 0.1 mM Thiamin diphosphate, 50 µM FAD, 60 mM KCl, 0.5 mM DTT, 10% Me2SO4 (v/v)
PH: 7
Temperature: 30
- [acetolactate synthase(Enzyme) mutant AHASII F109L]

Benzaldehyde + Pyruvate -> CO2 + R_Phenylacetylcarbinol		 (( (( (( (Vc * Ki) ) * f) ) * B) ) / (( (A + (f * B)) ) * (Ki + B))) buffer: 0.1 M Hepes/KOH, 5 mM MgCl2, 0.1 mM Thiamin diphosphate, 50 µM FAD, 60 mM KCl, 0.5 mM DTT, 10% Me2SO4 (v/v)
PH: 7
Temperature: 30
- [acetolactate synthase(Enzyme) mutant AHASII F109W]

Benzaldehyde + Pyruvate -> R_Phenylacetylcarbinol + CO2		 (( (( (( (Vc * Ki) ) * f) ) * B) ) / (( (A + (f * B)) ) * (Ki + B))) buffer: 0.1 M Hepes/KOH, 5 mM MgCl2, 0.1 mM Thiamin diphosphate, 50 µM FAD, 60 mM KCl, 0.5 mM DTT, 10% Me2SO4 (v/v)
PH: 7
Temperature: 30
- [acetolactate synthase(Enzyme) mutant AHASII M250A]

Pyruvate + Benzaldehyde -> CO2 + R_Phenylacetylcarbinol		 (( (( (( (Vc * Ki) ) * f) ) * B) ) / (( (A + (f * B)) ) * (Ki + B))) buffer: 0.1 M Hepes/KOH, 5 mM MgCl2, 0.1 mM Thiamin diphosphate, 50 µM FAD, 60 mM KCl, 0.5 mM DTT, 10% Me2SO4 (v/v)
PH: 7
Temperature: 30
- [acetolactate synthase(Enzyme) mutant AHASII R276M]

Pyruvate + Benzaldehyde -> CO2 + R_Phenylacetylcarbinol		 (( (( (( (Vc * Ki) ) * f) ) * B) ) / (( (A + (f * B)) ) * (Ki + B))) buffer: 0.1 M Hepes/KOH, 5 mM MgCl2, 0.1 mM Thiamin diphosphate, 50 µM FAD, 60 mM KCl, 0.5 mM DTT, 10% Me2SO4 (v/v)
PH: 7
Temperature: 30
- [2-Oxobutyrate]

Pyruvate -> CO2 + (S)_2_Acetolactate		 (( (Va * A) ) / (A + (R * B))) buffer: 0.1 M potassium phosphate, 10 mM MgCl2, 0.1 mM Thiamin diphosphate, 75 µM FAD
PH: 7.6
Temperature: 37
- [2-Oxobutyrate]

Pyruvate -> CO2 + (S)_2_Acetolactate		 (( (Va * A) ) / (A + (R * B))) buffer: 0.1 M potassium phosphate, 10 mM MgCl2, 0.1 mM Thiamin diphosphate, 75 µM FAD
PH: 7.6
Temperature: 37
- [2-Oxobutyrate]

Pyruvate -> CO2 + (S)_2_Acetolactate		 (( (Va * A) ) / (A + (R * B))) buffer: 0.1 M potassium phosphate, 10 mM MgCl2, 0.1 mM Thiamin diphosphate, 75 µM FAD
PH: 7.6
Temperature: 37
- [2-Oxobutyrate]

Pyruvate -> CO2 + (S)_2_Acetolactate		 (( (Va * A) ) / (A + (R * B))) buffer: 0.1 M potassium phosphate, 10 mM MgCl2, 0.1 mM Thiamin diphosphate, 75 µM FAD
PH: 7.6
Temperature: 37
- [2-Oxobutyrate]

Pyruvate -> CO2 + (S)_2_Acetolactate		 (( (Va * A) ) / (A + (R * B))) buffer: 0.1 M potassium phosphate, 10 mM MgCl2, 0.1 mM Thiamin diphosphate, 75 µM FAD
PH: 7.6
Temperature: 37
- [acetolactate synthase(Enzyme) mutant AHASII W464A]

Benzaldehyde + Pyruvate -> CO2 + R_Phenylacetylcarbinol		 (( (( (( (Vc * Ki) ) * f) ) * B) ) / (( (A + (f * B)) ) * (Ki + B))) buffer: 0.1 M Hepes/KOH, 5 mM MgCl2, 0.1 mM Thiamin diphosphate, 50 µM FAD, 60 mM KCl, 0.5 mM DTT, 10% Me2SO4 (v/v)
PH: 7
Temperature: 30
- [acetolactate synthase(Enzyme) mutant AHASII W464Q]

Benzaldehyde + Pyruvate -> CO2 + R_Phenylacetylcarbinol		 (( (( (( (Vc * Ki) ) * f) ) * B) ) / (( (A + (f * B)) ) * (Ki + B))) buffer: 0.1 M Hepes/KOH, 5 mM MgCl2, 0.1 mM Thiamin diphosphate, 50 µM FAD, 60 mM KCl, 0.5 mM DTT, 10% Me2SO4 (v/v)
PH: 7
Temperature: 30
- [acetolactate synthase(Enzyme) mutant AHASII W464L]

Pyruvate + Benzaldehyde -> CO2 + R_Phenylacetylcarbinol		 (( (( (( (Vc * Ki) ) * f) ) * B) ) / (( (A + (f * B)) ) * (Ki + B))) buffer: 0.1 M Hepes/KOH, 5 mM MgCl2, 0.1 mM Thiamin diphosphate, 50 µM FAD, 60 mM KCl, 0.5 mM DTT, 10% Me2SO4 (v/v)
PH: 7
Temperature: 30
- [acetolactate synthase(Enzyme) mutant AHASII W464Y]

Benzaldehyde + Pyruvate -> R_Phenylacetylcarbinol + CO2		 (( (( (( (Vc * Ki) ) * f) ) * B) ) / (( (A + (f * B)) ) * (Ki + B))) buffer: 0.1 M Hepes/KOH, 5 mM MgCl2, 0.1 mM Thiamin diphosphate, 50 µM FAD, 60 mM KCl, 0.5 mM DTT, 10% Me2SO4 (v/v)
PH: 7
Temperature: 30
- [2-Oxobutyrate]

Pyruvate -> CO2 + (S)_2_Acetolactate		 (( (Va * A) ) / (A + (R * B))) buffer: 0.1 M potassium phosphate, 10 mM MgCl2, 0.1 mM Thiamin diphosphate, 75 µM FAD
PH: 7.6
Temperature: 37
- [2-Oxobutyrate]

Pyruvate -> CO2 + (S)_2_Acetolactate		 (( (Va * A) ) / (A + (R * B))) buffer: 0.1 M potassium phosphate, 10 mM MgCl2, 0.1 mM Thiamin diphosphate, 75 µM FAD
PH: 7.6
Temperature: 37
- [2-Oxobutyrate]

Pyruvate -> CO2 + (S)_2_Acetolactate		 (( (Va * A) ) / (A + (R * B))) buffer: 0.1 M potassium phosphate, 10 mM MgCl2, 0.1 mM Thiamin diphosphate, 75 µM FAD
PH: 7.6
Temperature: 37
- [2-Oxobutyrate]

Pyruvate -> (S)_2_Acetolactate + CO2		 (( (Va * A) ) / (A + (R * B))) buffer: 0.1 M potassium phosphate, 10 mM MgCl2, 0.1 mM Thiamin diphosphate, 75 µM FAD
PH: 7.6
Temperature: 37
- [2-Oxobutyrate]

Pyruvate -> (S)_2_Acetolactate + CO2		 (( (Va * A) ) / (A + (R * B))) buffer: 0.1 M potassium phosphate, 10 mM MgCl2, 0.1 mM Thiamin diphosphate, 75 µM FAD
PH: 7.6
Temperature: 37
- [2-Oxobutyrate]

Pyruvate -> (S)_2_Acetolactate + CO2		 (( (Va * A) ) / (A + (R * B))) buffer: 0.1 M potassium phosphate, 10 mM MgCl2, 0.1 mM Thiamin diphosphate, 75 µM FAD
PH: 7.6
Temperature: 37
- [2-Oxobutyrate]

Pyruvate -> (S)_2_Acetolactate + CO2		 (( (Va * A) ) / (A + (R * B))) buffer: 0.1 M potassium phosphate, 10 mM MgCl2, 0.1 mM Thiamin diphosphate, 75 µM FAD
PH: 7.6
Temperature: 37
- [acetolactate synthase(Enzyme) mutant AHASII F109M]

Pyruvate + 2_Oxobutyrate -> CO2 + (S)_2_Aceto_2_hydroxybutanoate		 (( (( (Va * R) ) * B) ) / (A + (R * B))) buffer: 0.1 M potassium phosphate, 10 mM MgCl2, 0.1 mM Thiamin diphosphate, 75 µM FAD
PH: 7.6
Temperature: 37
- [acetolactate synthase(Enzyme) mutant AHASII F109L]

Pyruvate + 2_Oxobutyrate -> (S)_2_Aceto_2_hydroxybutanoate + CO2		 (( (( (Va * R) ) * B) ) / (A + (R * B))) buffer: 0.1 M potassium phosphate, 10 mM MgCl2, 0.1 mM Thiamin diphosphate, 75 µM FAD
PH: 7.6
Temperature: 37
- [acetolactate synthase(Enzyme) mutant AHASII R276K]

2_Oxobutyrate + Pyruvate -> CO2 + (S)_2_Aceto_2_hydroxybutanoate		 (( (( (Va * R) ) * B) ) / (A + (R * B))) buffer: 0.1 M potassium phosphate, 10 mM MgCl2, 0.1 mM Thiamin diphosphate, 75 µM FAD
PH: 7.6
Temperature: 37
- [acetolactate synthase(Enzyme) mutant AHASII R276F]

2_Oxobutyrate + Pyruvate -> (S)_2_Aceto_2_hydroxybutanoate + CO2		 (( (( (Va * R) ) * B) ) / (A + (R * B))) buffer: 0.1 M potassium phosphate, 10 mM MgCl2, 0.1 mM Thiamin diphosphate, 75 µM FAD
PH: 7.6
Temperature: 37
- [acetolactate synthase(Enzyme) mutant AHASII R276Q]

Pyruvate + 2_Oxobutyrate -> CO2 + (S)_2_Aceto_2_hydroxybutanoate		 (( (( (Va * R) ) * B) ) / (A + (R * B))) buffer: 0.1 M potassium phosphate, 10 mM MgCl2, 0.1 mM Thiamin diphosphate, 75 µM FAD
PH: 7.6
Temperature: 37
- [acetolactate synthase(Enzyme) mutant AHASII R276M]

2_Oxobutyrate + Pyruvate -> CO2 + (S)_2_Aceto_2_hydroxybutanoate		 (( (( (Va * R) ) * B) ) / (A + (R * B))) buffer: 0.1 M potassium phosphate, 10 mM MgCl2, 0.1 mM Thiamin diphosphate, 75 µM FAD
PH: 7.6
Temperature: 37
- [acetolactate synthase(Enzyme) mutant AHASII W464A]

Pyruvate + 2_Oxobutyrate -> (S)_2_Aceto_2_hydroxybutanoate + CO2		 (( (( (Va * R) ) * B) ) / (A + (R * B))) buffer: 0.1 M potassium phosphate, 10 mM MgCl2, 0.1 mM Thiamin diphosphate, 75 µM FAD
PH: 7.6
Temperature: 37
- [acetolactate synthase(Enzyme) mutant AHASII F109W]

2_Oxobutyrate + Pyruvate -> CO2 + (S)_2_Aceto_2_hydroxybutanoate		 (( (( (Va * R) ) * B) ) / (A + (R * B))) buffer: 0.1 M potassium phosphate, 10 mM MgCl2, 0.1 mM Thiamin diphosphate, 75 µM FAD
PH: 7.6
Temperature: 37
- [acetolactate synthase(Enzyme) mutant AHASII W464L]

2_Oxobutyrate + Pyruvate -> (S)_2_Aceto_2_hydroxybutanoate + CO2		 (( (( (Va * R) ) * B) ) / (A + (R * B))) buffer: 0.1 M potassium phosphate, 10 mM MgCl2, 0.1 mM Thiamin diphosphate, 75 µM FAD
PH: 7.6
Temperature: 37
- [acetolactate synthase(Enzyme) mutant AHASII M250A]

2_Oxobutyrate + Pyruvate -> (S)_2_Aceto_2_hydroxybutanoate + CO2		 (( (( (Va * R) ) * B) ) / (A + (R * B))) buffer: 0.1 M potassium phosphate, 10 mM MgCl2, 0.1 mM Thiamin diphosphate, 75 µM FAD
PH: 7.6
Temperature: 37
- [acetolactate synthase(Enzyme) mutant AHASII W464Q]

2_Oxobutyrate + Pyruvate -> (S)_2_Aceto_2_hydroxybutanoate + CO2		 (( (( (Va * R) ) * B) ) / (A + (R * B))) buffer: 0.1 M potassium phosphate, 10 mM MgCl2, 0.1 mM Thiamin diphosphate, 75 µM FAD
PH: 7.6
Temperature: 37
- [acetolactate synthase(Enzyme) mutant AHASII W464Y]

Pyruvate + 2_Oxobutyrate -> (S)_2_Aceto_2_hydroxybutanoate + CO2		 (( (( (Va * R) ) * B) ) / (A + (R * B))) buffer: 0.1 M potassium phosphate, 10 mM MgCl2, 0.1 mM Thiamin diphosphate, 75 µM FAD
PH: 7.6
Temperature: 37
- [acetolactate synthase(Enzyme) wildtype]

2 Pyruvate -> CO2 + 2_Acetolactate		 (( (V * substrate) ) / (Km + substrate)) buffer: 100 mM 2-(N-Morpholino)ethanesulfonic acid at pH 6.0-6.5, 100 mM 4-Morpholinopropanesulfonic acid at pH 6.5-8.0, 100 mM 3-[[Tris(hydroxymethyl)methyl]amino]propanesulfonic acid at pH 8.0-9.5, 1 mM ThDP, 10 mM MgCl2, 20 µM FAD
PH: 6
Temperature: 37
- [acetolactate synthase(Enzyme) wildtype]

2 Pyruvate -> CO2 + 2_Acetolactate		 (( (V * substrate) ) / (Km + substrate)) buffer: 100 mM 2-(N-Morpholino)ethanesulfonic acid at pH 6.0-6.5, 100 mM 4-Morpholinopropanesulfonic acid at pH 6.5-8.0, 100 mM 3-[[Tris(hydroxymethyl)methyl]amino]propanesulfonic acid at pH 8.0-9.5, 1 mM ThDP, 10 mM MgCl2, 20 µM FAD, D2O
PH: 6
Temperature: 37
- [Thiamine diphosphate]

2 Pyruvate -> CO2 + 2_Acetolactate		 (( (Vmax * (A ^ h)) ) / (( (Km ^ h) ) + (A ^ h))) buffer: *
PH: 7
Temperature: 36
- [Thiamine diphosphate]

2 Pyruvate -> 2_Acetolactate + CO2		 (( (Vmax * A) ) / (Km + A)) buffer: *
PH: 7
Temperature: 36
uniprot:P0AEP7 [FAD]

2 Pyruvate -> 2_Acetolactate + CO2		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M HEPES/KOH, 60 mM KCl, 0.5 mM DTT
PH: 7
Temperature: 30
uniprot:P0AEP7 [FAD]

2 Pyruvate -> CO2 + 2_Acetolactate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M HEPES/KOH, 60 mM KCl, 0.5 mM DTT
PH: 7
Temperature: 30
uniprot:P0AEP7 [FAD]

2 Pyruvate -> 2_Acetolactate + CO2		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M HEPES/KOH, 60 mM KCl, 0.5 mM DTT
PH: 7
Temperature: 30
uniprot:P0AEP7 [FAD]

2 Pyruvate -> CO2 + 2_Acetolactate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M HEPES/KOH, 60 mM KCl, 0.5 mM DTT
PH: 7
Temperature: 30
uniprot:P0AEP7 [FAD]

2 Pyruvate -> 2_Acetolactate + CO2		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M HEPES/KOH, 60 mM KCl, 0.5 mM DTT
PH: 7
Temperature: 30
uniprot:P0AEP7 [FAD]

2 Pyruvate -> 2_Acetolactate + CO2		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M HEPES/KOH, 60 mM KCl, 0.5 mM DTT
PH: 7
Temperature: 30

Pathways

pathway id name
BioCyc:META_P125-PWY superpathway of (R,R)-butanediol biosynthesis
BioCyc:META_PWY-6396 superpathway of 2,3-butanediol biosynthesis
BioCyc:ECO_ILEUSYN-PWY L-isoleucine biosynthesis I (from threonine)
BioCyc:MTBH37RV_ILEUSYN-PWY L-isoleucine biosynthesis I (from threonine)
BioCyc:MTBH37RV_PWY-3001 superpathway of L-isoleucine biosynthesis I
BioCyc:MTBH37RV_VALSYN-PWY L-valine biosynthesis
BioCyc:ECOO157_PWY-3001 isoleucine biosynthesis I
BioCyc:BSUB_P125-PWY superpathway of (R,R)-butanediol biosynthesis
BioCyc:BSUB_PWY-5939 pyruvate fermentation to (R)-acetoin II
BioCyc:ECOL316407_VALSYN-PWY valine biosynthesis
BioCyc:CORYNE_ILEUSYN-PWY L-isoleucine biosynthesis I (from threonine)
BioCyc:CORYNE_BRANCHED-CHAIN-AA-SYN-PWY superpathway of branched amino acid biosynthesis
BioCyc:CORYNE_PWY-6389 (S)-acetoin biosynthesis
BioCyc:META_PWY-5939 pyruvate fermentation to (R)-acetoin II
BioCyc:META_THREOCAT-PWY superpathway of L-threonine metabolism
BioCyc:META_ILEUSYN-PWY L-isoleucine biosynthesis I (from threonine)
BioCyc:ECO_BRANCHED-CHAIN-AA-SYN-PWY superpathway of branched chain amino acid biosynthesis
BioCyc:ECO_THREOCAT-PWY superpathway of L-threonine metabolism
BioCyc:YEAST_PWY-5938 (R)-acetoin biosynthesis I
BioCyc:ARA_ILEUSYN-PWY L-isoleucine biosynthesis I (from threonine)
BioCyc:ARA_BRANCHED-CHAIN-AA-SYN-PWY superpathway of branched chain amino acid biosynthesis
BioCyc:ARA_ILE-VAL-PWY superpathway of isoleucine and valine biosynthesis
BioCyc:MTBCDC1551_VALSYN-PWY L-valine biosynthesis
BioCyc:MTBCDC1551_BRANCHED-CHAIN-AA-SYN-PWY superpathway of branched amino acid biosynthesis
BioCyc:ECOO157_PWY-5103 isoleucine biosynthesis III
BioCyc:ECOO157_VALSYN-PWY valine biosynthesis
BioCyc:ECOL199310_PWY-3001 isoleucine biosynthesis I
BioCyc:ECOL199310_ILEUSYN-PWY isoleucine biosynthesis I (from threonine)
BioCyc:ECOL199310_VALSYN-PWY valine biosynthesis
BioCyc:CORYNE_PWY-5938 (R)-acetoin biosynthesis I
BioCyc:META_VALSYN-PWY L-valine biosynthesis
BioCyc:META_BRANCHED-CHAIN-AA-SYN-PWY superpathway of branched chain amino acid biosynthesis
BioCyc:META_PWY-5101 L-isoleucine biosynthesis II
BioCyc:ECO_VALSYN-PWY L-valine biosynthesis
BioCyc:YEAST_ILEUSYN-PWY isoleucine biosynthesis
BioCyc:MTBH37RV_BRANCHED-CHAIN-AA-SYN-PWY superpathway of branched chain amino acid biosynthesis
BioCyc:MTBCDC1551_ILEUSYN-PWY L-isoleucine biosynthesis I (from threonine)
BioCyc:ECOO157_ILEUSYN-PWY isoleucine biosynthesis I (from threonine)
BioCyc:ECOL199310_BRANCHED-CHAIN-AA-SYN-PWY superpathway of leucine, valine, and isoleucine biosynthesis
BioCyc:BSUB_BRANCHED-CHAIN-AA-SYN-PWY superpathway of branched chain amino acid biosynthesis
BioCyc:BSUB_ILEUSYN-PWY L-isoleucine biosynthesis I (from threonine)
BioCyc:BSUB_PWY-5938 pyruvate fermentation to (R)-acetoin I
BioCyc:ECOL316407_ILEUSYN-PWY isoleucine biosynthesis I (from threonine)
BioCyc:ECOL316407_BRANCHED-CHAIN-AA-SYN-PWY superpathway of leucine, valine, and isoleucine biosynthesis
BioCyc:CORYNE_VALSYN-PWY L-valine biosynthesis
BioCyc:META_PWY-5938 pyruvate fermentation to (R)-acetoin I
BioCyc:META_PWY-6389 pyruvate fermentation to (S)-acetoin
BioCyc:META_PWY-7111 pyruvate fermentation to isobutanol (engineered)
BioCyc:META_PWY-3001 superpathway of L-isoleucine biosynthesis I
BioCyc:YEAST_BRANCHED-CHAIN-AA-SYN-PWY superpathway of leucine, valine, and isoleucine biosynthesis
BioCyc:YEAST_VALSYN-PWY valine biosynthesis
BioCyc:ARA_PWY-3001 superpathway of L-isoleucine biosynthesis I
BioCyc:ARA_VALSYN-PWY L-valine biosynthesis
BioCyc:ECOO157_BRANCHED-CHAIN-AA-SYN-PWY superpathway of leucine, valine, and isoleucine biosynthesis
BioCyc:BSUB_VALSYN-PWY L-valine biosynthesis
BioCyc:ECOL316407_THREOCAT-PWY superpathway of threonine metabolism
BioCyc:CORYNE_PWY-3001 superpathway of L-isoleucine biosynthesis I
PathBank:SMP0000832 Valine Biosynthesis
PathBank:SMP0000838 Isoleucine Biosynthesis
PathBank:SMP0000994 Secondary Metabolites: Valine and L-Leucine Biosynthesis from Pyruvate
PathBank:SMP0001904 2-Oxopent-4-enoate Metabolism
PathBank:SMP0002049 2-Oxopent-4-enoate Metabolism 2
PlantCyc:ARA_VALSYN-PWY L-valine biosynthesis
PlantCyc:PLANT_ILE-VAL-PWY superpathway of isoleucine and valine biosynthesis
PlantCyc:ARA_PWY-3001 superpathway of L-isoleucine biosynthesis I
PlantCyc:ARA_BRANCHED-CHAIN-AA-SYN-PWY superpathway of branched chain amino acid biosynthesis
PlantCyc:ARA_ILEUSYN-PWY L-isoleucine biosynthesis I (from threonine)
PlantCyc:PLANT_VALSYN-PWY L-valine biosynthesis
PlantCyc:PLANT_PWY-3001 superpathway of L-isoleucine biosynthesis I
PlantCyc:PLANT_ILEUSYN-PWY L-isoleucine biosynthesis I (from threonine)
PathBank:SMP0012440 C5-Branched Dibasic Acid Metabolism