- [acetyl-CoA carboxytransferase(Enzyme) wildtype P0A9Q5 with C-terminal His-tag]
N6_D_Biotinyl_L_lysine + Malonyl_CoA -> N6_D_Carboxybiotinyl_L_lysine + Acetyl_CoA |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 100 mM Tris-Cl, 10 mM L-Malate, 0.6 mg/ml BSA, 3.6 units/ml malic dehydrogenase, 6.8 units/ml citrate synthase
PH: 8
Temperature: 25 |
- [acetyl-CoA carboxytransferase(Enzyme) mutant P0A9Q5 C27A, P0A9Q5 with C-terminal His-tag]
N6_D_Biotinyl_L_lysine + Malonyl_CoA -> N6_D_Carboxybiotinyl_L_lysine + Acetyl_CoA |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 100 mM Tris-Cl, 10 mM L-Malate, 0.6 mg/ml BSA, 3.6 units/ml malic dehydrogenase, 6.8 units/ml citrate synthase
PH: 8
Temperature: 25 |
- [acetyl-CoA carboxytransferase(Enzyme) wildtype P0A9Q5 with C-terminal His-tag]
Malonyl_CoA + N6_D_Biotinyl_L_lysine -> Acetyl_CoA + N6_D_Carboxybiotinyl_L_lysine |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 100 mM Tris-Cl, 10 mM L-Malate, 0.6 mg/ml BSA, 3.6 units/ml malic dehydrogenase, 6.8 units/ml citrate synthase
PH: 8
Temperature: 25 |
- [acetyl-CoA carboxytransferase(Enzyme) mutant P0A9Q5 C46A, P0A9Q5 with C-terminal His-tag]
N6_D_Biotinyl_L_lysine + Malonyl_CoA -> Acetyl_CoA + N6_D_Carboxybiotinyl_L_lysine |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 100 mM Tris-Cl, 10 mM L-Malate, 0.6 mg/ml BSA, 3.6 units/ml malic dehydrogenase, 6.8 units/ml citrate synthase
PH: 8
Temperature: 25 |
- [acetyl-CoA carboxytransferase(Enzyme) mutant P0A9Q5 C30A, P0A9Q5 with C-terminal His-tag]
N6_D_Biotinyl_L_lysine + Malonyl_CoA -> N6_D_Carboxybiotinyl_L_lysine + Acetyl_CoA |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 100 mM Tris-Cl, 10 mM L-Malate, 0.6 mg/ml BSA, 3.6 units/ml malic dehydrogenase, 6.8 units/ml citrate synthase
PH: 8
Temperature: 25 |
- [acetyl-CoA carboxytransferase(Enzyme) mutant P0A9Q5 C30A, P0A9Q5 with C-terminal His-tag]
N6_D_Biotinyl_L_lysine + Malonyl_CoA -> N6_D_Carboxybiotinyl_L_lysine + Acetyl_CoA |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 100 mM Tris-Cl, 10 mM L-Malate, 0.6 mg/ml BSA, 3.6 units/ml malic dehydrogenase, 6.8 units/ml citrate synthase
PH: 8
Temperature: 25 |
- [acetyl-CoA carboxytransferase(Enzyme) mutant P0A9Q5 C27A, P0A9Q5 with C-terminal His-tag]
N6_D_Biotinyl_L_lysine + Malonyl_CoA -> N6_D_Carboxybiotinyl_L_lysine + Acetyl_CoA |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 100 mM Tris-Cl, 10 mM L-Malate, 0.6 mg/ml BSA, 3.6 units/ml malic dehydrogenase, 6.8 units/ml citrate synthase
PH: 8
Temperature: 25 |
- [acetyl-CoA carboxytransferase(Enzyme) mutant P0A9Q5 C49A, P0A9Q5 with C-terminal His-tag]
Malonyl_CoA + N6_D_Biotinyl_L_lysine -> Acetyl_CoA + N6_D_Carboxybiotinyl_L_lysine |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 100 mM Tris-Cl, 10 mM L-Malate, 0.6 mg/ml BSA, 3.6 units/ml malic dehydrogenase, 6.8 units/ml citrate synthase
PH: 8
Temperature: 25 |
- [acetyl-CoA carboxytransferase(Enzyme) mutant P0A9Q5 C46A, P0A9Q5 with C-terminal His-tag]
N6_D_Biotinyl_L_lysine + Malonyl_CoA -> Acetyl_CoA + N6_D_Carboxybiotinyl_L_lysine |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 100 mM Tris-Cl, 10 mM L-Malate, 0.6 mg/ml BSA, 3.6 units/ml malic dehydrogenase, 6.8 units/ml citrate synthase
PH: 8
Temperature: 25 |
- [acetyl-CoA carboxytransferase(Enzyme) mutant P0A9Q5 C49A, P0A9Q5 with C-terminal His-tag]
Malonyl_CoA + N6_D_Biotinyl_L_lysine -> Acetyl_CoA + N6_D_Carboxybiotinyl_L_lysine |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 100 mM Tris-Cl, 10 mM L-Malate, 0.6 mg/ml BSA, 3.6 units/ml malic dehydrogenase, 6.8 units/ml citrate synthase
PH: 8
Temperature: 25 |
- [Biotin]
Hydrogencarbonate + Acetyl_CoA + ATP -> Malonyl_CoA + Phosphate + ADP |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 100 mM HEPES, 0.2 mM NADH, 0.5 mM phosphoenolpyruvate, 20 U/ml pyruvate kinase, 36 U/ml lactate dehydrogenase
PH: 8
Temperature: 25 |