EC: 2.1.2.11

3-methyl-2-oxobutanoate hydroxymethyltransferase (5,10-methylenetetrahydrofolate:3-methyl-2-oxobutanoate hydroxymethyltransferase)

enzyme lambda metabolic reaction experiment
uniprot:P31057 [3-methyl-2-oxobutanoate hydroxymethyltransferase(Enzyme) wildtype]

2_Dehydropantoate + 5,6,7,8_Tetrahydrofolate -> alpha_Ketovaline + 5,10_Methylenetetrahydrofolate + H2O		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 1 M potassium phosphate, 1.4 mM MgSO4
PH: 6.8
Temperature: 37
uniprot:P31057 [3-methyl-2-oxobutanoate hydroxymethyltransferase(Enzyme) wildtype]

5,6,7,8_Tetrahydrofolate + 2_Dehydropantoate -> 5,10_Methylenetetrahydrofolate + H2O + alpha_Ketovaline		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 1 M potassium phosphate, 1.4 mM MgSO4
PH: 6.8
Temperature: 37
uniprot:P31057 [3-methyl-2-oxobutanoate hydroxymethyltransferase(Enzyme) wildtype]

Tetrahydrofolyl_[Glu](2) + 2_Dehydropantoate -> H2O + alpha_Ketovaline + 5,10_Methylenetetrahydrofolyl_[Glu](2)		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 1 M potassium phosphate, 1.4 mM MgSO4
PH: 6.8
Temperature: 37
uniprot:P31057 [3-methyl-2-oxobutanoate hydroxymethyltransferase(Enzyme) wildtype]

2_Dehydropantoate + Tetrahydrofolyl_[Glu](6) -> 5,10_Methylenetetrahydrofolyl_[Glu](6) + alpha_Ketovaline + H2O		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 1 M potassium phosphate, 1.4 mM MgSO4
PH: 6.8
Temperature: 37
uniprot:P31057 [3-methyl-2-oxobutanoate hydroxymethyltransferase(Enzyme) wildtype]

2_Dehydropantoate + Tetrahydrofolyl_[Glu](7) -> H2O + alpha_Ketovaline + 5,10_Methylenetetrahydrofolyl_[Glu](7)		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 1 M potassium phosphate, 1.4 mM MgSO4
PH: 6.8
Temperature: 37
uniprot:P31057 [3-methyl-2-oxobutanoate hydroxymethyltransferase(Enzyme) wildtype]

2_Dehydropantoate + Tetrahydrofolyl_[Glu](3) -> H2O + alpha_Ketovaline + 5,10_Methylenetetrahydrofolyl_[Glu](3)		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 1 M potassium phosphate, 1.4 mM MgSO4
PH: 6.8
Temperature: 37
uniprot:P31057 [3-methyl-2-oxobutanoate hydroxymethyltransferase(Enzyme) wildtype]

Tetrahydrofolyl_[Glu](5) + 2_Dehydropantoate -> H2O + alpha_Ketovaline + 5,10_Methylenetetrahydrofolyl_[Glu](5)		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 1 M potassium phosphate, 1.4 mM MgSO4
PH: 6.8
Temperature: 37
uniprot:P31057 [3-methyl-2-oxobutanoate hydroxymethyltransferase(Enzyme) wildtype]

2_Dehydropantoate + Tetrahydrofolyl_[Glu](4) -> alpha_Ketovaline + 5,10_Methylenetetrahydrofolyl_[Glu](4) + H2O		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 1 M potassium phosphate, 1.4 mM MgSO4
PH: 6.8
Temperature: 37

Pathways

pathway id name
BioCyc:VCHO_PANTO-PWY phosphopantothenate biosynthesis I
BioCyc:ECOO157_PANTO-PWY phosphopantothenate biosynthesis I
BioCyc:SYNEL_PANTO-PWY phosphopantothenate biosynthesis I
BioCyc:ECOL316407_PANTO-PWY phosphopantothenate biosynthesis I
BioCyc:CAULONA1000_PANTO-PWY pantothenate biosynthesis I
BioCyc:GCF_000013425_PANTOSYN-PWY superpathway of coenzyme A biosynthesis I (bacteria)
BioCyc:META_PWY-4221 superpathway of coenzyme A biosynthesis II (plants)
BioCyc:ECO_PANTO-PWY phosphopantothenate biosynthesis I
BioCyc:CAULONA1000_PANTOSYN-PWY pantothenate and coenzyme A biosynthesis I
BioCyc:META_PANTOSYN-PWY superpathway of coenzyme A biosynthesis I (bacteria)
BioCyc:YEAST_PANTO-PWY phosphopantothenate biosynthesis
BioCyc:MTBH37RV_PANTO-PWY phosphopantothenate biosynthesis I
BioCyc:MTBCDC1551_PANTOSYN-PWY pantothenate and coenzyme A biosynthesis I
BioCyc:MTBCDC1551_PANTO-PWY phosphopantothenate biosynthesis I
BioCyc:HPY_PANTO-PWY phosphopantothenate biosynthesis I
BioCyc:ECOL199310_PANTO-PWY phosphopantothenate biosynthesis I
BioCyc:ANTHRA_PANTOSYN-PWY pantothenate and coenzyme A biosynthesis I
BioCyc:AGRO_PANTO-PWY phosphopantothenate biosynthesis I
BioCyc:BSUB_PANTO-PWY phosphopantothenate biosynthesis I
BioCyc:PDIF272563_PANTO-PWY phosphopantothenate biosynthesis I
BioCyc:BTHE_PANTO-PWY phosphopantothenate biosynthesis I
BioCyc:META_PANTO-PWY phosphopantothenate biosynthesis I
BioCyc:ECO_PANTOSYN-PWY superpathway of coenzyme A biosynthesis I (bacteria)
BioCyc:ARA_PWY-4221ARA superpathway of pantothenate and coenzymeA biosynthesis
BioCyc:ARA_PANTO-PWY phosphopantothenate biosynthesis I
BioCyc:ECOL199310_PANTOSYN-PWY pantothenate and coenzyme A biosynthesis I
BioCyc:ANTHRA_PANTO-PWY phosphopantothenate biosynthesis I
BioCyc:AGRO_PANTOSYN-PWY superpathway of coenzyme A biosynthesis I (bacteria)
BioCyc:BSUB_PANTOSYN-PWY superpathway of coenzyme A biosynthesis I (bacteria)
BioCyc:ECOL316407_PANTOSYN-PWY pantothenate and coenzyme A biosynthesis I
BioCyc:CORYNE_PANTOSYN-PWY pantothenate and coenzyme A biosynthesis I
BioCyc:CORYNE_PANTO-PWY phosphopantothenate biosynthesis I
BioCyc:GCF_000013425_PANTO-PWY phosphopantothenate biosynthesis I
PathBank:SMP0000848 Pantothenate and CoA Biosynthesis
PlantCyc:ARA_PANTO-PWY phosphopantothenate biosynthesis I
PlantCyc:PLANT_PWY-4221 superpathway of coenzyme A biosynthesis II (plants)
PlantCyc:ARA_PWY-4221ARA superpathway of pantothenate and coenzymeA biosynthesis
PlantCyc:ARA_PWY-4221 superpathway of coenzyme A biosynthesis II (plants)
PlantCyc:PLANT_PANTO-PWY phosphopantothenate biosynthesis I
PlantCyc:PLANT_PWY-4221ARA superpathway of pantothenate and coenzymeA biosynthesis
PathBank:SMP0121296 Pantothenate and CoA Biosynthesis
PathBank:SMP0002364 Pantothenate and CoA Biosynthesis