EC: 2.1.1.68

caffeate O-methyltransferase (S-adenosyl-L-methionine:3,4-dihydroxy-trans-cinnamate 3-O-methyltransferase)

enzyme lambda metabolic reaction experiment
uniprot:Q9XGW0 [caffeate O-methyltransferase(Enzyme) wildtype]

Caffeate + S_Adenosyl_L_methionine -> Ferulate + S_Adenosyl_L_homocysteine		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM bis-Tris, 10% glycerol, 5 mM Na2S2O5, 10 mM 2-mercaptoethanol, 1 mM EDTA
PH: 7
Temperature: 30
uniprot:Q9XGW0 [caffeate O-methyltransferase(Enzyme) wildtype]

S_Adenosyl_L_methionine + Caffeate -> Ferulate + S_Adenosyl_L_homocysteine		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM bis-Tris, 10% glycerol, 5 mM Na2S2O5, 10 mM 2-mercaptoethanol, 1 mM EDTA
PH: 7
Temperature: 30
uniprot:Q9XGW0 [caffeate O-methyltransferase(Enzyme) wildtype]

Catechol + S_Adenosyl_L_methionine -> S_Adenosyl_L_homocysteine + Guaiacol		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM bis-Tris, 10% glycerol, 5 mM Na2S2O5, 10 mM 2-mercaptoethanol, 1 mM EDTA
PH: 7
Temperature: 30
uniprot:Q38J50 [caffeate O-methyltransferase(Enzyme) wildtype]

Caffeic_aldehyde + S_Adenosyl_L_methionine -> S_Adenosyl_L_homocysteine + Coniferaldehyde		 (( (Vmax * S) ) / (Km + S)) buffer: 200 mM Tris-HCl, 14 mM 2-mercaptoethanol
PH: 7.2
Temperature: 30
uniprot:Q38J50 [caffeate O-methyltransferase(Enzyme) wildtype]

5_Hydroxyconiferaldehyde + S_Adenosyl_L_methionine -> S_Adenosyl_L_homocysteine + Sinapoyl_aldehyde		 (( (Vmax * S) ) / (Km + S)) buffer: 200 mM Tris-HCl, 14 mM 2-mercaptoethanol
PH: 7.2
Temperature: 30
uniprot:Q38J50 [caffeate O-methyltransferase(Enzyme) wildtype]

Caffeyl_alcohol + S_Adenosyl_L_methionine -> S_Adenosyl_L_homocysteine + Coniferyl_alcohol		 (( (Vmax * S) ) / (Km + S)) buffer: 200 mM Tris-HCl, 14 mM 2-mercaptoethanol
PH: 7.2
Temperature: 30
uniprot:Q38J50 [caffeate O-methyltransferase(Enzyme) wildtype]

S_Adenosyl_L_methionine + 5_Hydroxyconiferyl_alcohol -> Sinapyl_alcohol + S_Adenosyl_L_homocysteine		 (( (Vmax * S) ) / (Km + S)) buffer: 200 mM Tris-HCl, 14 mM 2-mercaptoethanol
PH: 7.2
Temperature: 30
uniprot:Q38J50 [caffeate O-methyltransferase(Enzyme) wildtype]

Caffeate + S_Adenosyl_L_methionine -> S_Adenosyl_L_homocysteine + Ferulate		 (( (Vmax * S) ) / (Km + S)) buffer: 200 mM Tris-HCl, 14 mM 2-mercaptoethanol
PH: 7.2
Temperature: 30

Pathways

pathway id name
BioCyc:META_PWY-7995 tricin biosynthesis
BioCyc:META_PWY-6631 tricetin methylation
BioCyc:META_PWY-6232 chrysoeriol biosynthesis
Plant Reactome:R-OSA-9826798 Gravitropism under normal or artificial gravity environments
Plant Reactome:R-OSA-9928372 Root gravitropism
Plant Reactome:R-OSA-9916190 Root angle formation: elongation and curvature response
Plant Reactome:R-OSA-8932729 Responses to stimuli: abiotic stimuli and stresses
Plant Reactome:R-OSA-9609573 Tricin biosynthesis
Plant Reactome:R-OSA-2744345 Metabolism and regulation
Plant Reactome:R-OSA-2744344 Secondary metabolism
PlantCyc:ORYZA_PWY-7995 tricin biosynthesis
PlantCyc:ORYZA_PWY-6199 quercetin sulfate biosynthesis
PlantCyc:PLANT_PWY-5710 capsaicin biosynthesis
PlantCyc:PLANT_PWY-7995 tricin biosynthesis
PlantCyc:ORYZA_PWY-6631 tricetin methylation
PlantCyc:ORYZA_PWY-6232 chrysoeriol biosynthesis
PlantCyc:ORYZA_PWY-6064 methylquercetin biosynthesis
PathBank:SMP0012025 Flavone and Flavonol Biosynthesis