EC: 2.1.1.2

guanidinoacetate N-methyltransferase (S-adenosyl-L-methionine:N-guanidinoacetate methyltransferase)

enzyme lambda metabolic reaction experiment
uniprot:P10868 [guanidinoacetate N-methyltransferase(Enzyme) mutant D129N]

S_Adenosyl_L_methionine + Guanidinoacetate -> Creatine + S_Adenosyl_L_homocysteine		 (( (( (E * kcat) ) * A) ) / (Ka + A)) buffer: 0.1 M potassium phosphate, recombinant rat S-Adenosyl-L-homocysteine hydrolase, calf intestinal mucosa adenosine deaminase
PH: 8
Temperature: 30
uniprot:P10868 [guanidinoacetate N-methyltransferase(Enzyme) wildtype]

Guanidinoacetate + S_Adenosyl_L_methionine -> Creatine + S_Adenosyl_L_homocysteine		 (( (( (E * kcat) ) * A) ) / (Ka + A)) buffer: 0.1 M potassium phosphate, recombinant rat S-Adenosyl-L-homocysteine hydrolase, calf intestinal mucosa adenosine deaminase
PH: 8
Temperature: 30
uniprot:P10868 [guanidinoacetate N-methyltransferase(Enzyme) mutant D129A]

S_Adenosyl_L_methionine + Guanidinoacetate -> Creatine + S_Adenosyl_L_homocysteine		 (( (( (E * kcat) ) * A) ) / (Ka + A)) buffer: 0.1 M potassium phosphate, recombinant rat S-Adenosyl-L-homocysteine hydrolase, calf intestinal mucosa adenosine deaminase
PH: 8
Temperature: 30
uniprot:P10868 [guanidinoacetate N-methyltransferase(Enzyme) mutant Y133F]

S_Adenosyl_L_methionine + Guanidinoacetate -> S_Adenosyl_L_homocysteine + Creatine		 (( (( (E * kcat) ) * A) ) / (Ka + A)) buffer: 0.1 M potassium phosphate, recombinant rat S-Adenosyl-L-homocysteine hydrolase, calf intestinal mucosa adenosine deaminase
PH: 8
Temperature: 30
uniprot:P10868 [guanidinoacetate N-methyltransferase(Enzyme) mutant D134N]

Guanidinoacetate + S_Adenosyl_L_methionine -> Creatine + S_Adenosyl_L_homocysteine		 (( (( (E * kcat) ) * A) ) / (Ka + A)) buffer: 0.1 M potassium phosphate, recombinant rat S-Adenosyl-L-homocysteine hydrolase, calf intestinal mucosa adenosine deaminase
PH: 8
Temperature: 30
uniprot:P10868 [guanidinoacetate N-methyltransferase(Enzyme) mutant T135A]

S_Adenosyl_L_methionine + Guanidinoacetate -> Creatine + S_Adenosyl_L_homocysteine		 (( (( (E * kcat) ) * A) ) / (Ka + A)) buffer: 0.1 M potassium phosphate, recombinant rat S-Adenosyl-L-homocysteine hydrolase, calf intestinal mucosa adenosine deaminase
PH: 8
Temperature: 30
uniprot:P10868 [guanidinoacetate N-methyltransferase(Enzyme) mutant D129N]

Guanidinoacetate + S_Adenosyl_L_methionine -> Creatine + S_Adenosyl_L_homocysteine		 (( (( (E * kcat) ) * B) ) / (Kb + B)) buffer: 0.1 M potassium phosphate, recombinant rat S-Adenosyl-L-homocysteine hydrolase, calf intestinal mucosa adenosine deaminase
PH: 8
Temperature: 30
uniprot:P10868 [guanidinoacetate N-methyltransferase(Enzyme) mutant D134E]

Guanidinoacetate + S_Adenosyl_L_methionine -> Creatine + S_Adenosyl_L_homocysteine		 (( (( (E * kcat) ) * A) ) / (Ka + A)) buffer: 0.1 M potassium phosphate, recombinant rat S-Adenosyl-L-homocysteine hydrolase, calf intestinal mucosa adenosine deaminase
PH: 8
Temperature: 30
uniprot:P10868 [guanidinoacetate N-methyltransferase(Enzyme) mutant D129A]

Guanidinoacetate + S_Adenosyl_L_methionine -> S_Adenosyl_L_homocysteine + Creatine		 (( (( (E * kcat) ) * B) ) / (Kb + B)) buffer: 0.1 M potassium phosphate, recombinant rat S-Adenosyl-L-homocysteine hydrolase, calf intestinal mucosa adenosine deaminase
PH: 8
Temperature: 30
uniprot:P10868 [guanidinoacetate N-methyltransferase(Enzyme) wildtype]

S_Adenosyl_L_methionine + Guanidinoacetate -> S_Adenosyl_L_homocysteine + Creatine		 (( (( (E * kcat) ) * B) ) / (Kb + B)) buffer: 0.1 M potassium phosphate, recombinant rat S-Adenosyl-L-homocysteine hydrolase, calf intestinal mucosa adenosine deaminase
PH: 8
Temperature: 30
uniprot:P10868 [guanidinoacetate N-methyltransferase(Enzyme) mutant Y136F]

Guanidinoacetate + S_Adenosyl_L_methionine -> S_Adenosyl_L_homocysteine + Creatine		 (( (( (E * kcat) ) * A) ) / (Ka + A)) buffer: 0.1 M potassium phosphate, recombinant rat S-Adenosyl-L-homocysteine hydrolase, calf intestinal mucosa adenosine deaminase
PH: 8
Temperature: 30
uniprot:P10868 [Sinefungin]

Guanidinoacetate + S_Adenosyl_L_methionine -> S_Adenosyl_L_homocysteine + Creatine		 (( (( (E * kcat) ) * A) ) / (( (Ka * (1 + (I / Ki))) ) + A)) buffer: 0.1 M potassium phosphate, recombinant rat S-Adenosyl-L-homocysteine hydrolase, calf intestinal mucosa adenosine deaminase
PH: 8
Temperature: 30
uniprot:P10868 [guanidinoacetate N-methyltransferase(Enzyme) wildtype]

S_Adenosyl_L_methionine + Guanidinoacetate -> S_Adenosyl_L_homocysteine + Creatine		 (( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (( (Kia * Kb) ) + (Ka * B)) ) + (Kb * A)) ) + (A * B))) buffer: 0.1 M potassium phosphate, recombinant rat S-Adenosyl-L-homocysteine hydrolase, calf intestinal mucosa adenosine deaminase
PH: 8
Temperature: 30
uniprot:P10868 [guanidinoacetate N-methyltransferase(Enzyme) wildtype]

Guanidinoacetate + S_Adenosyl_L_methionine -> Creatine + S_Adenosyl_L_homocysteine		 (( (( (E * kcat) ) * A) ) / (( (Ka * (1 + (I / Ki))) ) + A)) buffer: 0.1 M potassium phosphate
PH: 8
Temperature: 30
uniprot:P10868 [guanidinoacetate N-methyltransferase(Enzyme) mutant Y133F]

S_Adenosyl_L_methionine + Guanidinoacetate -> Creatine + S_Adenosyl_L_homocysteine		 (( (( (E * kcat) ) * B) ) / (Kb + B)) buffer: 0.1 M potassium phosphate, recombinant rat S-Adenosyl-L-homocysteine hydrolase, calf intestinal mucosa adenosine deaminase
PH: 8
Temperature: 30
uniprot:P10868 [guanidinoacetate N-methyltransferase(Enzyme) mutant D134N]

S_Adenosyl_L_methionine + Guanidinoacetate -> S_Adenosyl_L_homocysteine + Creatine		 (( (( (E * kcat) ) * B) ) / (Kb + B)) buffer: 0.1 M potassium phosphate, recombinant rat S-Adenosyl-L-homocysteine hydrolase, calf intestinal mucosa adenosine deaminase
PH: 8
Temperature: 30
uniprot:P10868 [guanidinoacetate N-methyltransferase(Enzyme) mutant T135A]

Guanidinoacetate + S_Adenosyl_L_methionine -> Creatine + S_Adenosyl_L_homocysteine		 (( (( (E * kcat) ) * B) ) / (Kb + B)) buffer: 0.1 M potassium phosphate, recombinant rat S-Adenosyl-L-homocysteine hydrolase, calf intestinal mucosa adenosine deaminase
PH: 8
Temperature: 30
uniprot:P10868 [guanidinoacetate N-methyltransferase(Enzyme) mutant D134E]

S_Adenosyl_L_methionine + Guanidinoacetate -> Creatine + S_Adenosyl_L_homocysteine		 (( (( (E * kcat) ) * B) ) / (Kb + B)) buffer: 0.1 M potassium phosphate, recombinant rat S-Adenosyl-L-homocysteine hydrolase, calf intestinal mucosa adenosine deaminase
PH: 8
Temperature: 30
uniprot:P10868 [guanidinoacetate N-methyltransferase(Enzyme) mutant Y136F]

S_Adenosyl_L_methionine + Guanidinoacetate -> Creatine + S_Adenosyl_L_homocysteine		 (( (( (E * kcat) ) * B) ) / (Kb + B)) buffer: 0.1 M potassium phosphate, recombinant rat S-Adenosyl-L-homocysteine hydrolase, calf intestinal mucosa adenosine deaminase
PH: 8
Temperature: 30

Pathways

pathway id name
BioCyc:META_GLYCGREAT-PWY creatine biosynthesis
BioCyc:MOUSE_PWY3DJ-35588 creatine biosynthesis
BioCyc:MOUSE_GLYCGREAT-PWY glycine degradation (creatine biosynthesis)
BioCyc:HUMAN_GLYCGREAT-PWY creatine biosynthesis
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-71291 Amino acid and derivative metabolism
Reactome:R-DRE-71291 Amino acid and derivative metabolism
Reactome:R-HSA-71288 Creatine metabolism
Reactome:R-HSA-74160 Gene expression (Transcription)
Reactome:R-HSA-73857 RNA Polymerase II Transcription
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-71288 Creatine metabolism
Reactome:R-RNO-71288 Creatine metabolism
Reactome:R-BTA-71288 Creatine metabolism
Reactome:R-DRE-71288 Creatine metabolism
Reactome:R-HSA-8986944 Transcriptional Regulation by MECP2
Reactome:R-XTR-71291 Amino acid and derivative metabolism
Reactome:R-DRE-1430728 Metabolism
Reactome:R-HSA-71291 Amino acid and derivative metabolism
Reactome:R-HSA-212436 Generic Transcription Pathway
Reactome:R-MMU-71291 Amino acid and derivative metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-71291 Amino acid and derivative metabolism
Reactome:R-XTR-1430728 Metabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-XTR-71288 Creatine metabolism
WikiPathways:WP497 Urea cycle and metabolism of amino groups
WikiPathways:WP4583 Biomarkers for urea cycle disorders
PathBank:SMP0120694 Dihydropyrimidine Dehydrogenase Deficiency (DHPD)
PathBank:SMP0120710 Guanidinoacetate Methyltransferase Deficiency (GAMT Deficiency)
PathBank:SMP0120754 Non-Ketotic Hyperglycinemia
PathBank:SMP0120758 Ornithine Aminotransferase Deficiency (OAT Deficiency)
PathBank:SMP0120770 Hyperglycinemia, Non-Ketotic
PathBank:SMP0120790 Hyperornithinemia-Hyperammonemia-Homocitrullinuria [HHH-syndrome]
PathBank:SMP0120569 Creatine Deficiency, Guanidinoacetate Methyltransferase Deficiency
PathBank:SMP0000020 Arginine and Proline Metabolism
PathBank:SMP0000362 Arginine: Glycine Amidinotransferase Deficiency (AGAT Deficiency)
PathBank:SMP0000188 Guanidinoacetate Methyltransferase Deficiency (GAMT Deficiency)
PathBank:SMP0000242 Dimethylglycine Dehydrogenase Deficiency
PathBank:SMP0000223 Non-Ketotic Hyperglycinemia
PathBank:SMP0000484 Dimethylglycine Dehydrogenase Deficiency
PathBank:SMP0000504 Creatine Deficiency, Guanidinoacetate Methyltransferase Deficiency
PathBank:SMP0087338 Glycine and Serine Metabolism
PathBank:SMP0063596 Arginine and Proline Metabolism
PathBank:SMP0120653 3-Phosphoglycerate Dehydrogenase Deficiency
PathBank:SMP0120721 Hyperprolinemia Type II
PathBank:SMP0120741 Sarcosinemia
PathBank:SMP0120761 Prolidase Deficiency (PD)
PathBank:SMP0120789 Hyperornithinemia with Gyrate Atrophy (HOGA)
PathBank:SMP0120475 Dimethylglycine Dehydrogenase Deficiency
PathBank:SMP0120499 Hyperprolinemia Type I
PathBank:SMP0120539 Prolinemia Type II
PathBank:SMP0120572 L-Arginine:Glycine Amidinotransferase Deficiency
PathBank:SMP0000004 Glycine and Serine Metabolism
PathBank:SMP0000207 Prolidase Deficiency (PD)
PathBank:SMP0000208 Prolinemia Type II
PathBank:SMP0000507 L-Arginine:Glycine Amidinotransferase Deficiency
PathBank:SMP0000721 3-Phosphoglycerate Dehydrogenase Deficiency
PathBank:SMP0087245 Glycine and Serine Metabolism
PathBank:SMP0120788 Creatine Deficiency, Guanidinoacetate Methyltransferase Deficiency
PathBank:SMP0120872 3-Phosphoglycerate Dehydrogenase Deficiency
PathBank:SMP0120501 Hyperprolinemia Type II
PathBank:SMP0120521 Sarcosinemia
PathBank:SMP0120541 Prolidase Deficiency (PD)
PathBank:SMP0120570 Hyperornithinemia with Gyrate Atrophy (HOGA)
PathBank:SMP0000360 Hyperprolinemia Type II
PathBank:SMP0000363 Ornithine Aminotransferase Deficiency (OAT Deficiency)
PathBank:SMP0000179 Dihydropyrimidine Dehydrogenase Deficiency (DHPD)
PathBank:SMP0000485 Hyperglycinemia, Non-Ketotic
PathBank:SMP0000505 Hyperornithinemia with Gyrate Atrophy (HOGA)
PathBank:SMP0120675 Arginine: Glycine Amidinotransferase Deficiency (AGAT Deficiency)
PathBank:SMP0120695 Dimethylglycine Dehydrogenase Deficiency
PathBank:SMP0120719 Hyperprolinemia Type I
PathBank:SMP0120759 Prolinemia Type II
PathBank:SMP0120791 L-Arginine:Glycine Amidinotransferase Deficiency
PathBank:SMP0120454 Arginine: Glycine Amidinotransferase Deficiency (AGAT Deficiency)
PathBank:SMP0120474 Dihydropyrimidine Dehydrogenase Deficiency (DHPD)
PathBank:SMP0120490 Guanidinoacetate Methyltransferase Deficiency (GAMT Deficiency)
PathBank:SMP0120534 Non-Ketotic Hyperglycinemia
PathBank:SMP0120538 Ornithine Aminotransferase Deficiency (OAT Deficiency)
PathBank:SMP0120550 Hyperglycinemia, Non-Ketotic
PathBank:SMP0120571 Hyperornithinemia-Hyperammonemia-Homocitrullinuria [HHH-syndrome]
PathBank:SMP0000361 Hyperprolinemia Type I
PathBank:SMP0000244 Sarcosinemia
PathBank:SMP0000506 Hyperornithinemia-Hyperammonemia-Homocitrullinuria [HHH-syndrome]
PathBank:SMP0087178 Arginine and Proline Metabolism
PathBank:SMP0087283 Arginine and Proline Metabolism
PathBank:SMP0063626 Glycine and Serine Metabolism