EC: 2.1.1.159

theobromine synthase (S-adenosyl-L-methionine:7-methylxanthine N3-methyltransferase)

enzyme lambda metabolic reaction experiment
uniprot:Q9AVJ9 [theobromine synthase(Enzyme) wildtype GST-fusion]

1,7_Dimethylxanthine + S_Adenosyl_L_methionine -> S_Adenosyl_L_homocysteine + Caffeine
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 200 µM MgCl2
PH: 8
Temperature: 27
uniprot:Q9AVJ9 [theobromine synthase(Enzyme) wildtype GST-fusion]

7_Methylxanthosine + S_Adenosyl_L_methionine -> Theobromine + S_Adenosyl_L_homocysteine
(( (Vmax * B) ) / (Km + B)) buffer: 50 mM Tris-HCl, 200 µM MgCl2
PH: 8
Temperature: 27
uniprot:Q9AVJ9 [theobromine synthase(Enzyme) wildtype GST-fusion]

7_Methylxanthosine + S_Adenosyl_L_methionine -> S_Adenosyl_L_homocysteine + Theobromine
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 200 µM MgCl2
PH: 8
Temperature: 27
uniprot:Q84PP7 [theobromine synthase(Enzyme) wildtype GST-fusion]

1,7_Dimethylxanthine + S_Adenosyl_L_methionine -> S_Adenosyl_L_homocysteine + Caffeine
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 200 µM MgCl2
PH: 8
Temperature: 27
uniprot:Q84PP7 [theobromine synthase(Enzyme) wildtype GST-fusion]

7_Methylxanthosine + S_Adenosyl_L_methionine -> Theobromine + S_Adenosyl_L_homocysteine
(( (Vmax * B) ) / (Km + B)) buffer: 50 mM Tris-HCl, 200 µM MgCl2
PH: 8
Temperature: 27
uniprot:Q84PP7 [theobromine synthase(Enzyme) wildtype GST-fusion]

S_Adenosyl_L_methionine + 7_Methylxanthosine -> Theobromine + S_Adenosyl_L_homocysteine
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 200 µM MgCl2
PH: 8
Temperature: 27
uniprot:Q9AVJ9 [theobromine synthase(Enzyme) wildtype GST-fusion]

7_Methylxanthine + S_Adenosyl_L_methionine -> Theobromine + S_Adenosyl_L_homocysteine
(( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 200 µM MgCl2
PH: 8.3
Temperature: 27
uniprot:Q9AVJ9 [theobromine synthase(Enzyme) wildtype GST-fusion]

7_Methylxanthine + S_Adenosyl_L_methionine -> S_Adenosyl_L_homocysteine + Theobromine
(( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 200 µM MgCl2
PH: 8.3
Temperature: 27

Pathways

pathway id name