EC: 1.8.3.1

sulfite oxidase (sulfite:oxygen oxidoreductase)

enzyme lambda metabolic reaction experiment
uniprot:P51687 [sulfite oxidase(Enzyme) mutant G473D]

2 Cytochrome_c + H2O + Sulfite -> 2 Reduced_cytochrome_c + 2 H+ + Sulfate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM glycine
PH: 9.1
Temperature: 25
uniprot:P51687 [sulfite oxidase(Enzyme) mutant G473D]

2 Cytochrome_c + H2O + Sulfite -> 2 H+ + Sulfate + 2 Reduced_cytochrome_c		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Bis-Tris propane
PH: 7.5
Temperature: 25
uniprot:P51687 [sulfite oxidase(Enzyme) mutant G473D]

H2O + 2 Cytochrome_c + Sulfite -> Sulfate + 2 H+ + 2 Reduced_cytochrome_c		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Bis-Tris
PH: 6
Temperature: 25
uniprot:P51687 [sulfite oxidase(Enzyme) mutant G473D]

Sulfite + H2O + 2 Cytochrome_c -> Sulfate + 2 H+ + 2 Reduced_cytochrome_c		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Bis-Tris propane
PH: 7
Temperature: 25
uniprot:P51687 [sulfite oxidase(Enzyme) mutant G473D]

2 Cytochrome_c + Sulfite + H2O -> Sulfate + 2 H+ + 2 Reduced_cytochrome_c		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Bis-Tris
PH: 6.5
Temperature: 25
uniprot:P51687 [sulfite oxidase(Enzyme) mutant G473D]

2 Cytochrome_c + H2O + Sulfite -> Sulfate + 2 H+ + 2 Reduced_cytochrome_c		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Tris
PH: 8
Temperature: 25
uniprot:P51687 [sulfite oxidase(Enzyme) mutant G473D]

2 Cytochrome_c + H2O + Sulfite -> 2 H+ + Sulfate + 2 Reduced_cytochrome_c		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Tris
PH: 8.5
Temperature: 25
uniprot:P51687 [sulfite oxidase(Enzyme) mutant G473A]

2 Cytochrome_c + H2O + Sulfite -> Sulfate + 2 H+ + 2 Reduced_cytochrome_c		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Bis-Tris propane
PH: 7.4
Temperature: 25
uniprot:P51687 [sulfite oxidase(Enzyme) mutant G473A]

Sulfite + H2O + 2 Cytochrome_c -> 2 Reduced_cytochrome_c + Sulfate + 2 H+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Tris
PH: 8.5
Temperature: 25
uniprot:P51687 [sulfite oxidase(Enzyme) mutant G473A]

Sulfite + 2 Cytochrome_c + H2O -> 2 H+ + 2 Reduced_cytochrome_c + Sulfate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Bis-Tris propane
PH: 7
Temperature: 25
uniprot:P51687 [sulfite oxidase(Enzyme) mutant G473A]

2 Cytochrome_c + H2O + Sulfite -> 2 Reduced_cytochrome_c + Sulfate + 2 H+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Tris
PH: 8
Temperature: 25
uniprot:P51687 [sulfite oxidase(Enzyme) mutant G473A]

2 Cytochrome_c + H2O + Sulfite -> 2 Reduced_cytochrome_c + Sulfate + 2 H+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM glycine
PH: 9.1
Temperature: 25
uniprot:P51687 [sulfite oxidase(Enzyme) mutant G473W]

2 Cytochrome_c + Sulfite + H2O -> Sulfate + 2 H+ + 2 Reduced_cytochrome_c		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Bis-Tris
PH: 6
Temperature: 25
uniprot:P51687 [sulfite oxidase(Enzyme) mutant G473A]

H2O + 2 Cytochrome_c + Sulfite -> 2 Reduced_cytochrome_c + 2 H+ + Sulfate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Bis-Tris
PH: 6
Temperature: 25
uniprot:P51687 [sulfite oxidase(Enzyme) mutant G473W]

2 Cytochrome_c + H2O + Sulfite -> Sulfate + 2 Reduced_cytochrome_c + 2 H+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Bis-Tris propane
PH: 7
Temperature: 25
uniprot:P51687 [sulfite oxidase(Enzyme) mutant G473A]

2 Cytochrome_c + Sulfite + H2O -> 2 H+ + Sulfate + 2 Reduced_cytochrome_c		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM glycine
PH: 10
Temperature: 25
uniprot:P51687 [sulfite oxidase(Enzyme) mutant G473W]

H2O + Sulfite + 2 Cytochrome_c -> 2 H+ + Sulfate + 2 Reduced_cytochrome_c		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM glycine
PH: 9
Temperature: 25
uniprot:P51687 [sulfite oxidase(Enzyme) mutant G473W]

Sulfite + H2O + 2 Cytochrome_c -> 2 H+ + 2 Reduced_cytochrome_c + Sulfate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Tris
PH: 8.5
Temperature: 25
uniprot:P51687 [sulfite oxidase(Enzyme) wildtype]

2 Reduced_cytochrome_c + Sulfate + 2 H+ -> 2 Cytochrome_c + Sulfite + H2O		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Bis-Tris propane
PH: 7
Temperature: 25
uniprot:P51687 [sulfite oxidase(Enzyme) wildtype]

2 H+ + Sulfate + 2 Reduced_cytochrome_c -> 2 Cytochrome_c + Sulfite + H2O		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Tris
PH: 8.5
Temperature: 25
uniprot:P51687 [sulfite oxidase(Enzyme) mutant G473D]

2 H+ + 2 Reduced_cytochrome_c + Sulfate -> 2 Cytochrome_c + Sulfite + H2O		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Bis-Tris propane
PH: 7
Temperature: 25
uniprot:P51687 [sulfite oxidase(Enzyme) wildtype]

2 Reduced_cytochrome_c + 2 H+ + Sulfate -> H2O + Sulfite + 2 Cytochrome_c		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Bis-Tris
PH: 6
Temperature: 25
uniprot:P51687 [sulfite oxidase(Enzyme) mutant G473D]

2 H+ + 2 Reduced_cytochrome_c + Sulfate -> Sulfite + H2O + 2 Cytochrome_c		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Bis-Tris
PH: 6
Temperature: 25
uniprot:P51687 [sulfite oxidase(Enzyme) mutant G473D]

2 Reduced_cytochrome_c + 2 H+ + Sulfate -> 2 Cytochrome_c + H2O + Sulfite		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Bis-Tris propane
PH: 7.5
Temperature: 25
uniprot:P51687 [sulfite oxidase(Enzyme) mutant G473D]

2 Reduced_cytochrome_c + Sulfate + 2 H+ -> Sulfite + H2O + 2 Cytochrome_c		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Bis-Tris
PH: 6.5
Temperature: 25
uniprot:P51687 [sulfite oxidase(Enzyme) mutant G473D]

2 Reduced_cytochrome_c + 2 H+ + Sulfate -> Sulfite + 2 Cytochrome_c + H2O		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Tris
PH: 8.6
Temperature: 25
uniprot:P51687 [sulfite oxidase(Enzyme) mutant G473A]

Sulfate + 2 H+ + 2 Reduced_cytochrome_c -> Sulfite + 2 Cytochrome_c + H2O		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Tris
PH: 8.5
Temperature: 25
uniprot:P51687 [sulfite oxidase(Enzyme) mutant G473A]

2 Reduced_cytochrome_c + 2 H+ + Sulfate -> Sulfite + H2O + 2 Cytochrome_c		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Bis-Tris
PH: 6
Temperature: 25
uniprot:P51687 [sulfite oxidase(Enzyme) mutant G473D]

2 Reduced_cytochrome_c + Sulfate + 2 H+ -> Sulfite + H2O + 2 Cytochrome_c		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Tris
PH: 8
Temperature: 25
uniprot:P51687 [sulfite oxidase(Enzyme) mutant G473W]

2 Reduced_cytochrome_c + 2 H+ + Sulfate -> 2 Cytochrome_c + H2O + Sulfite		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Tris
PH: 8.5
Temperature: 25
uniprot:P51687 [sulfite oxidase(Enzyme) mutant G473W]

2 Reduced_cytochrome_c + 2 H+ + Sulfate -> H2O + 2 Cytochrome_c + Sulfite		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Bis-Tris propane
PH: 7
Temperature: 25
uniprot:P51687 [sulfite oxidase(Enzyme) mutant G473W]

2 H+ + 2 Reduced_cytochrome_c + Sulfate -> 2 Cytochrome_c + H2O + Sulfite		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Bis-Tris
PH: 6
Temperature: 25
uniprot:P51687 [sulfite oxidase(Enzyme) mutant G473A]

2 Reduced_cytochrome_c + 2 H+ + Sulfate -> Sulfite + H2O + 2 Cytochrome_c		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Bis-Tris propane
PH: 7
Temperature: 25
uniprot:P51687 [sulfite oxidase(Enzyme) mutant G473D]

2 Hexacyanoferrate(III) + Sulfite + H2O -> Sulfate + 2 Hexacyanoferrate(II) + 2 H+		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Bis-Tris propane
PH: 7
Temperature: 25
uniprot:P51687 [sulfite oxidase(Enzyme) mutant G473D]

H2O + 2 Hexacyanoferrate(III) + Sulfite -> 2 H+ + Sulfate + 2 Hexacyanoferrate(II)		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Tris
PH: 8.5
Temperature: 25
uniprot:P51687 [sulfite oxidase(Enzyme) mutant G473A]

Sulfite + H2O + 2 Hexacyanoferrate(III) -> 2 Hexacyanoferrate(II) + 2 H+ + Sulfate		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Bis-Tris propane
PH: 7
Temperature: 25
uniprot:P51687 [sulfite oxidase(Enzyme) mutant G473W]

H2O + 2 Hexacyanoferrate(III) + Sulfite -> Sulfate + 2 Hexacyanoferrate(II) + 2 H+		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Tris
PH: 8.5
Temperature: 25
uniprot:P51687 [sulfite oxidase(Enzyme) mutant G473A]

H2O + Sulfite + 2 Hexacyanoferrate(III) -> Sulfate + 2 H+ + 2 Hexacyanoferrate(II)		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Tris
PH: 8.5
Temperature: 25
uniprot:P51687 [sulfite oxidase(Enzyme) mutant G473W]

Sulfite + H2O + 2 Hexacyanoferrate(III) -> 2 Hexacyanoferrate(II) + Sulfate + 2 H+		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Bis-Tris propane
PH: 7
Temperature: 25

Pathways

pathway id name
BioCyc:HUMAN_PWY-5326 sulfite oxidation
BioCyc:HUMAN_PWY-5328 superpathway of methionine degradation
BioCyc:HUMAN_PWY-7927 sulfide oxidation IV (metazoa)
BioCyc:META_PWY-5328 superpathway of L-methionine salvage and degradation
BioCyc:META_PWY-7927 sulfide oxidation IV (metazoa)
BioCyc:MOUSE_PWY-5328 superpathway of methionine degradation
BioCyc:MOUSE_PWY3DJ-35888 sulfur metabolism
BioCyc:MOUSE_PWY-5326 sulfite oxidation IV
BioCyc:META_PWY-5326 sulfite oxidation IV
Reactome:R-DME-1614558 Degradation of cysteine and homocysteine
Reactome:R-HSA-1614558 Degradation of cysteine and homocysteine
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-1614558 Degradation of cysteine and homocysteine
Reactome:R-RNO-1614558 Degradation of cysteine and homocysteine
Reactome:R-DME-1430728 Metabolism
Reactome:R-DME-71291 Amino acid and derivative metabolism
Reactome:R-DME-1614517 Sulfide oxidation to sulfate
Reactome:R-HSA-71291 Amino acid and derivative metabolism
Reactome:R-HSA-1614517 Sulfide oxidation to sulfate
Reactome:R-MMU-71291 Amino acid and derivative metabolism
Reactome:R-MMU-1614517 Sulfide oxidation to sulfate
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-71291 Amino acid and derivative metabolism
Reactome:R-RNO-1614517 Sulfide oxidation to sulfate
Reactome:R-DME-1614635 Sulfur amino acid metabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-1614635 Sulfur amino acid metabolism
Reactome:R-MMU-1614635 Sulfur amino acid metabolism
Reactome:R-RNO-1614635 Sulfur amino acid metabolism
WikiPathways:WP5355 Metabolic Epileptic Disorders
WikiPathways:WP4507 Molybdenum cofactor (Moco) biosynthesis
WikiPathways:WP5030 Ethylmalonic encephalopathy
PathBank:SMP0000041 Sulfate/Sulfite Metabolism
PathBank:SMP0000532 Sulfite Oxidase Deficiency
PathBank:SMP0087389 Sulfate/Sulfite Metabolism