EC: 1.5.3.17

non-specific polyamine oxidase (polyamine:oxygen oxidoreductase (3-aminopropanal or 3-acetamidopropanal-forming))

enzyme lambda metabolic reaction experiment
- [non-specific polyamine oxidase(Enzyme) wildtype]

O2 + H2O + Tryptamine -> NH3 + H2O2 + Indole_3_acetaldehyde
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 5 mM benzaldehyde, 10 µM dithiothreitol, 8 µg/ml homovanillic acid, 20 µg/ml horseradish peroxidase, 0.02% Brij 35
PH: 8.5
Temperature: 37
- [non-specific polyamine oxidase(Enzyme) wildtype]

O2 + Benzylamine + H2O -> NH3 + H2O2 + Benzaldehyde
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 5 mM benzaldehyde, 10 µM dithiothreitol, 8 µg/ml homovanillic acid, 20 µg/ml horseradish peroxidase, 0.02% Brij 35
PH: 8.5
Temperature: 37

Pathways

pathway id name
BioCyc:META_PWY-6441 spermine and spermidine degradation III
BioCyc:META_PWY-5760 β-alanine biosynthesis IV
BioCyc:YEAST_PWY-5760 β-alanine biosynthesis
BioCyc:ARA_PWY-6441 spermine and spermidine degradation III
PlantCyc:PLANT_PWY-6441 spermine and spermidine degradation III
PlantCyc:ARA_PWY-6441 spermine and spermidine degradation III
PathBank:SMP0002307 beta-Alanine Metabolism