EC: 1.4.3.22

diamine oxidase (histamine:oxygen oxidoreductase (deaminating))

enzyme lambda metabolic reaction experiment
- [diamine oxidase(Enzyme) wildtype]

O2 + Histamine + H2O -> Imidazole_4_acetaldehyde + H2O2 + NH3		 (( (V * substrate) ) / (Km + substrate)) buffer: 0.1 M potassium phosphate, at 120 mM ionic strength
PH: 7.2
Temperature: 25
- [diamine oxidase(Enzyme) wildtype]

H2O + Putrescine + O2 -> NH3 + 4_Aminobutanal + H2O2		 (( (V * substrate) ) / (Km + substrate)) buffer: 0.1 M potassium phosphate, 120 mM ionic strength
PH: 7.2
Temperature: 25
- [diamine oxidase(Enzyme) wildtype]

Cadaverine + H2O + O2 -> 5_Aminopentanal + H2O2 + NH3		 (( (V * substrate) ) / (Km + substrate)) buffer: 0.1 M potassium phosphate, 120 mM ionic strength
PH: 7.2
Temperature: 25
- [diamine oxidase(Enzyme) wildtype]

Agmatine + O2 + H2O -> 4_Guanidinobutanal + H2O2 + NH3		 (( (V * substrate) ) / (Km + substrate)) buffer: 0.1 M potassium phosphate, at 120 mM ionic strength
PH: 7.2
Temperature: 25
- [diamine oxidase(Enzyme) wildtype]

O2 + H2O + Spermidine -> NH3 + Unknown + H2O2		 (( (V * substrate) ) / (Km + substrate)) buffer: 0.1 M potassium phosphate, at 120 mM ionic strength
PH: 7.2
Temperature: 25
- [diamine oxidase(Enzyme) wildtype]

Spermine + H2O + O2 -> NH3 + H2O2 + Unknown		 (( (V * substrate) ) / (Km + substrate)) buffer: 0.1 M Tris/HCl, at 120 mM ionic strength
PH: 7.2
Temperature: 25
uniprot:Q9TRC7 [diamine oxidase(Enzyme) wildtype]

Putrescine + H2O + O2 -> H2O2 + 4_Aminobutanal + NH3		 (( (Vmax * S) ) / (Km + S)) buffer: 0.2 M phosphate, 1200 U/ml glutamate dehydrogenase, 285 mM 2-Oxoglutarate, 9.5 mM NADH
PH: 7.4
Temperature: 37
uniprot:Q9TRC7 [diamine oxidase(Enzyme) wildtype]

Cadaverine + O2 + H2O -> H2O2 + 5_Aminopentanal + NH3		 (( (Vmax * S) ) / (Km + S)) buffer: 0.2 M phosphate, 1200 U/ml glutamate dehydrogenase, 285 mM 2-Oxoglutarate, 9.5 mM NADH
PH: 7.4
Temperature: 37
uniprot:Q9TRC7 [diamine oxidase(Enzyme) wildtype]

H2O + O2 + N_Methylputrescine -> NH3 + 4_Methylaminobutanal + H2O2		 (( (Vmax * S) ) / (Km + S)) buffer: 0.2 M phosphate, 1200 U/ml glutamate dehydrogenase, 285 mM 2-Oxoglutarate, 9.5 mM NADH
PH: 7.4
Temperature: 37

Pathways

pathway id name
BioCyc:MOUSE_PWY-6181 histamine degradation
BioCyc:META_PWY-6181 histamine degradation
BioCyc:HUMAN_PWY-6181 histamine degradation
BioCyc:MOUSE_PWY-3981 β-alanine biosynthesis I
Reactome:R-HSA-211859 Biological oxidations
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-211859 Biological oxidations
Reactome:R-RNO-211945 Phase I - Functionalization of compounds
Reactome:R-RNO-211859 Biological oxidations
Reactome:R-HSA-211945 Phase I - Functionalization of compounds
Reactome:R-MMU-211945 Phase I - Functionalization of compounds
Reactome:R-RNO-1430728 Metabolism
Reactome:R-HSA-1430728 Metabolism
PathBank:SMP0000190 Hawkinsinuria
PathBank:SMP0000169 Alkaptonuria
PathBank:SMP0000429 Disulfiram Action Pathway
PathBank:SMP0063599 beta-Alanine Metabolism
PathBank:SMP0120558 Carnosinuria, Carnosinemia
PathBank:SMP0000044 Histidine Metabolism
PathBank:SMP0000191 Histidinemia
PathBank:SMP0000218 Tyrosinemia Type I
PathBank:SMP0000533 Monoamine Oxidase-A Deficiency (MAO-A)
PathBank:SMP0120478 GABA-Transaminase Deficiency
PathBank:SMP0000006 Tyrosine Metabolism
PathBank:SMP0000494 Tyrosinemia, Transient, of the Newborn
PathBank:SMP0000498 Dopamine beta-Hydroxylase Deficiency