EC: 1.4.1.4

glutamate dehydrogenase (NADP+) (L-glutamate:NADP+ oxidoreductase (deaminating))

enzyme lambda metabolic reaction experiment
- [glutamate dehydrogenase (NADP+)(Enzyme) wildtype]

NADPH + NH3 + H+ + 2_Oxoglutarate -> L_Glutamate + H2O + NADP+		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 8
Temperature: 25
- [glutamate dehydrogenase (NADP+)(Enzyme) wildtype]

H+ + NH3 + NADPH + 2_Oxoglutarate -> L_Glutamate + H2O + NADP+		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 8
Temperature: 25
- [glutamate dehydrogenase (NADP+)(Enzyme) wildtype]

NADPH + 2_Oxoglutarate + H+ + NH3 -> L_Glutamate + H2O + NADP+		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 8
Temperature: 25
- [glutamate dehydrogenase (NADP+)(Enzyme) wildtype]

H2O + L_Glutamate + NADP+ -> NADPH + H+ + 2_Oxoglutarate + NH3		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 8
Temperature: 25
- [glutamate dehydrogenase (NADP+)(Enzyme) wildtype]

H2O + NADP+ + L_Glutamate -> 2_Oxoglutarate + H+ + NADPH + NH3		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 8
Temperature: 25
uniprot:P15111 [glutamate dehydrogenase (NADP+)(Enzyme) mutant K286Q]

H+ + NADPH + NH3 + 2_Oxoglutarate -> NADP+ + L_Glutamate + H2O		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM potassium phosphate
PH: 8
Temperature: 25
uniprot:P15111 [glutamate dehydrogenase (NADP+)(Enzyme) wildtype]

NADPH + 2_Oxoglutarate + H+ + NH3 -> L_Glutamate + H2O + NADP+		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM potassium phosphate
PH: 8
Temperature: 25
uniprot:P15111 [glutamate dehydrogenase (NADP+)(Enzyme) mutant K286R]

NADPH + H+ + 2_Oxoglutarate + NH3 -> L_Glutamate + H2O + NADP+		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM potassium phosphate
PH: 8
Temperature: 25
uniprot:P15111 [glutamate dehydrogenase (NADP+)(Enzyme) mutant K286E]

NH3 + H+ + 2_Oxoglutarate + NADPH -> H2O + NADP+ + L_Glutamate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl
PH: 8.08
Temperature: 25
uniprot:P15111 [glutamate dehydrogenase (NADP+)(Enzyme) mutant K286E]

2_Oxoglutarate + NH3 + H+ + NADPH -> L_Glutamate + NADP+ + H2O		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate
PH: 7.07
Temperature: 25
uniprot:P15111 [glutamate dehydrogenase (NADP+)(Enzyme) mutant K286Q]

H+ + 2_Oxoglutarate + NADPH + NH3 -> L_Glutamate + NADP+ + H2O		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate
PH: 7.38
Temperature: 25
uniprot:P15111 [glutamate dehydrogenase (NADP+)(Enzyme) mutant K286Q]

2_Oxoglutarate + H+ + NH3 + NADPH -> H2O + NADP+ + L_Glutamate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate
PH: 6.69
Temperature: 25
uniprot:P15111 [glutamate dehydrogenase (NADP+)(Enzyme) mutant K286E]

NH3 + H+ + NADPH + 2_Oxoglutarate -> L_Glutamate + H2O + NADP+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate
PH: 6.69
Temperature: 25
uniprot:P15111 [glutamate dehydrogenase (NADP+)(Enzyme) mutant K286E]

H+ + 2_Oxoglutarate + NH3 + NADPH -> L_Glutamate + H2O + NADP+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate
PH: 7.38
Temperature: 25
uniprot:P15111 [glutamate dehydrogenase (NADP+)(Enzyme) mutant K286E]

NADPH + H+ + 2_Oxoglutarate + NH3 -> NADP+ + H2O + L_Glutamate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate
PH: 7.58
Temperature: 25
uniprot:P15111 [glutamate dehydrogenase (NADP+)(Enzyme) mutant K286Q]

NH3 + H+ + 2_Oxoglutarate + NADPH -> L_Glutamate + H2O + NADP+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate
PH: 6.3
Temperature: 25
uniprot:P15111 [glutamate dehydrogenase (NADP+)(Enzyme) mutant K286Q]

NADPH + NH3 + H+ + 2_Oxoglutarate -> L_Glutamate + H2O + NADP+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate
PH: 7.02
Temperature: 25
uniprot:P15111 [glutamate dehydrogenase (NADP+)(Enzyme) mutant K286R]

H+ + NH3 + NADPH + 2_Oxoglutarate -> L_Glutamate + H2O + NADP+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate
PH: 8
Temperature: 25
uniprot:P15111 [glutamate dehydrogenase (NADP+)(Enzyme) mutant K286Q]

NH3 + H+ + NADPH + 2_Oxoglutarate -> L_Glutamate + H2O + NADP+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate
PH: 8
Temperature: 25
uniprot:P15111 [glutamate dehydrogenase (NADP+)(Enzyme) mutant K286E]

NH3 + 2_Oxoglutarate + NADPH + H+ -> NADP+ + H2O + L_Glutamate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate
PH: 8
Temperature: 25
uniprot:P15111 [glutamate dehydrogenase (NADP+)(Enzyme) wildtype]

NH3 + H+ + NADPH + 2_Oxoglutarate -> H2O + L_Glutamate + NADP+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate
PH: 8
Temperature: 25
uniprot:P15111 [glutamate dehydrogenase (NADP+)(Enzyme) mutant K286E]

2_Oxoglutarate + NH3 + NADPH + H+ -> NADP+ + L_Glutamate + H2O		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM potassium phosphate
PH: 8
Temperature: 25
uniprot:P15111 [glutamate dehydrogenase (NADP+)(Enzyme) mutant K286E]

NADPH + H+ + 2_Oxoglutarate + NH3 -> L_Glutamate + NADP+ + H2O		 (( (( (E * kcat) ) * C) ) / (Km + C)) buffer: 50 mM potassium phosphate
PH: 8
Temperature: 25
uniprot:P15111 [glutamate dehydrogenase (NADP+)(Enzyme) wildtype]

H+ + NADPH + NH3 + 2_Oxoglutarate -> L_Glutamate + H2O + NADP+		 (( (( (E * kcat) ) * C) ) / (Km + C)) buffer: 50 mM potassium phosphate
PH: 8
Temperature: 25
uniprot:P15111 [glutamate dehydrogenase (NADP+)(Enzyme) mutant K286R]

NADPH + NH3 + H+ + 2_Oxoglutarate -> L_Glutamate + H2O + NADP+		 (( (( (E * kcat) ) * C) ) / (Km + C)) buffer: 50 mM potassium phosphate
PH: 8
Temperature: 25
uniprot:P15111 [glutamate dehydrogenase (NADP+)(Enzyme) mutant K286Q]

H+ + 2_Oxoglutarate + NH3 + NADPH -> L_Glutamate + H2O + NADP+		 (( (( (E * kcat) ) * C) ) / (Km + C)) buffer: 50 mM potassium phosphate
PH: 8
Temperature: 25
uniprot:P15111 [glutamate dehydrogenase (NADP+)(Enzyme) mutant K278Q]

H+ + NH3 + NADPH + 2_Oxoglutarate -> NADP+ + L_Glutamate + H2O		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate
PH: 8
Temperature: 25
uniprot:P15111 [glutamate dehydrogenase (NADP+)(Enzyme) mutant K278Q]

H+ + NH3 + NADPH + 2_Oxoglutarate -> L_Glutamate + NADP+ + H2O		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM potassium phosphate
PH: 8
Temperature: 25
uniprot:P15111 [glutamate dehydrogenase (NADP+)(Enzyme) mutant K278Q]

2_Oxoglutarate + NADPH + NH3 + H+ -> L_Glutamate + NADP+ + H2O		 (( (( (E * kcat) ) * C) ) / (Km + C)) buffer: 50 mM potassium phosphate
PH: 8
Temperature: 25
uniprot:P15111 [glutamate dehydrogenase (NADP+)(Enzyme) mutant K286R]

NADPH + H+ + 2_Oxoglutarate + NH3 -> NADP+ + L_Glutamate + H2O		 (Vmaxi / (1 + (( (10 ^ (0 - pH)) ) / (10 ^ (0 - pKaes))))) buffer: 50 mM potassium phosphate
PH: 8
Temperature: 25
uniprot:P15111 [glutamate dehydrogenase (NADP+)(Enzyme) wildtype]

2_Oxoglutarate + NH3 + NADPH + H+ -> L_Glutamate + NADP+ + H2O		 (Vmaxi / (1 + (( (10 ^ (0 - pH)) ) / (10 ^ (0 - pKaes))))) buffer: 50 mM potassium phosphate
PH: 8
Temperature: 25
uniprot:P15111 [glutamate dehydrogenase (NADP+)(Enzyme) mutant K286Q]

2_Oxoglutarate + NADPH + NH3 + H+ -> L_Glutamate + H2O + NADP+		 (Vmaxi / (1 + (( (10 ^ (0 - pH)) ) / (10 ^ (0 - pKaes))))) buffer: 50 mM potassium phosphate
PH: 8
Temperature: 25
uniprot:P15111 [glutamate dehydrogenase (NADP+)(Enzyme) mutant K286E]

NH3 + NADPH + H+ + 2_Oxoglutarate -> NADP+ + L_Glutamate + H2O		 (Vmaxi / (1 + (( (10 ^ (0 - pH)) ) / (10 ^ (0 - pKaes))))) buffer: 50 mM potassium phosphate
PH: 8
Temperature: 25
uniprot:P15111 [glutamate dehydrogenase (NADP+)(Enzyme) mutant K286Q]

NH3 + NADPH + H+ + 2_Oxoglutarate -> H2O + NADP+ + L_Glutamate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate
PH: 7.57
Temperature: 25
uniprot:P15111 [glutamate dehydrogenase (NADP+)(Enzyme) mutant K286Q]

H+ + 2_Oxoglutarate + NADPH + NH3 -> NADP+ + H2O + L_Glutamate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl
PH: 8.08
Temperature: 25
uniprot:P15111 [glutamate dehydrogenase (NADP+)(Enzyme) mutant K286Q]

NADH + H+ + NH3 + 2_Oxoglutarate -> H2O + L_Glutamate + NAD+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate
PH: 8
Temperature: 25
uniprot:P15111 [glutamate dehydrogenase (NADP+)(Enzyme) mutant K286R]

NH3 + 2_Oxoglutarate + NADH + H+ -> H2O + L_Glutamate + NAD+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate
PH: 8
Temperature: 25
uniprot:P15111 [glutamate dehydrogenase (NADP+)(Enzyme) mutant K286E]

NADH + H+ + NH3 + 2_Oxoglutarate -> L_Glutamate + H2O + NAD+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate
PH: 8
Temperature: 25
uniprot:P15111 [glutamate dehydrogenase (NADP+)(Enzyme) wildtype]

H+ + NADH + 2_Oxoglutarate + NH3 -> H2O + NAD+ + L_Glutamate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate
PH: 8
Temperature: 25
uniprot:P15111 [glutamate dehydrogenase (NADP+)(Enzyme) wildtype]

H+ + 2_Oxoglutarate + NADPH + NH3 -> H2O + L_Glutamate + NADP+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate
PH: 6.72
Temperature: 25
uniprot:P15111 [glutamate dehydrogenase (NADP+)(Enzyme) wildtype]

2_Oxoglutarate + H+ + NADPH + NH3 -> NADP+ + L_Glutamate + H2O		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate
PH: 7.4
Temperature: 25
uniprot:P15111 [glutamate dehydrogenase (NADP+)(Enzyme) mutant K286E]

NADPH + 2_Oxoglutarate + H+ + NH3 -> H2O + L_Glutamate + NADP+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate
PH: 6.31
Temperature: 25
uniprot:P15111 [glutamate dehydrogenase (NADP+)(Enzyme) wildtype]

2_Oxoglutarate + NH3 + H+ + NADPH -> H2O + L_Glutamate + NADP+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl
PH: 8.1
Temperature: 25
uniprot:P15111 [glutamate dehydrogenase (NADP+)(Enzyme) mutant K286E]

NADPH + H+ + 2_Oxoglutarate + NH3 -> H2O + L_Glutamate + NADP+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate
PH: 5.98
Temperature: 25

Pathways

pathway id name
BioCyc:META_PWY-5505 L-glutamate and L-glutamine biosynthesis
BioCyc:META_GLUTSYNIII-PWY L-glutamate biosynthesis III
BioCyc:ECO_GLUTSYNIII-PWY L-glutamate biosynthesis III
BioCyc:HPY_GLUTAMATE-SYN2-PWY L-glutamate biosynthesis II
BioCyc:HPY_GLUTSYNIII-PWY L-glutamate biosynthesis III
BioCyc:ECOL316407_GLUTSYNIII-PWY glutamate biosynthesis III
BioCyc:META_PWY-5675 nitrate reduction V (assimilatory)
BioCyc:YEAST_GLUTSYNIII-PWY glutamate biosynthesis from ammonia
BioCyc:HPY_PWY-5766 glutamate degradation X
BioCyc:HPY_GLUTAMATE-DEG1-PWY glutamate degradation I
BioCyc:CORYNE_GLUTSYNIII-PWY L-glutamate biosynthesis III
PathBank:SMP0000778 Nitrogen Metabolism
PathBank:SMP0000811 L-Glutamate Metabolism
PathBank:SMP0002403 Nitrogen Metabolism
PathBank:SMP0002303 Glutamate Metabolism
PathBank:SMP0002291 The Oncogenic Action of 2-Hydroxyglutarate