EC: 1.4.1.2

glutamate dehydrogenase (L-glutamate:NAD+ oxidoreductase (deaminating))

enzyme lambda metabolic reaction experiment
uniprot:P24295 [glutamate dehydrogenase(Enzyme) mutant A163G]

NADH + H+ + NH3 + 2_Oxoglutarate -> NAD+ + H2O + L_Glutamate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate
PH: 7
Temperature: 25
uniprot:P24295 [glutamate dehydrogenase(Enzyme) mutant S380A]

NH3 + 2_Oxoglutarate + NADH + H+ -> NAD+ + L_Glutamate + H2O		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate
PH: 7
Temperature: 25
uniprot:P24295 [glutamate dehydrogenase(Enzyme) wildtype]

H+ + NADH + 2_Oxoglutarate + NH3 -> L_Glutamate + H2O + NAD+		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate
PH: 7
Temperature: 25
uniprot:P24295 [glutamate dehydrogenase(Enzyme) mutant K89L/A163G]

2_Oxoglutarate + H+ + NADH + NH3 -> L_Glutamate + NAD+ + H2O		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate
PH: 7
Temperature: 25
uniprot:P24295 [glutamate dehydrogenase(Enzyme) mutant S380A]

L_Glutamate + NAD+ + H2O -> H+ + NADH + 2_Oxoglutarate + NH3		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M Tris/HCl
PH: 8
Temperature: 25
uniprot:P24295 [glutamate dehydrogenase(Enzyme) wildtype]

NAD+ + H2O + L_Glutamate -> NADH + H+ + NH3 + 2_Oxoglutarate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M Tris/HCl
PH: 8
Temperature: 25
uniprot:P24295 [glutamate dehydrogenase(Enzyme) mutant A163G]

NAD+ + H2O + L_Glutamate -> H+ + NH3 + 2_Oxoglutarate + NADH		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M Tris/HCl
PH: 8
Temperature: 25
uniprot:P24295 [glutamate dehydrogenase(Enzyme) mutant K89L/A163G]

NAD+ + L_Glutamate + H2O -> 2_Oxoglutarate + NADH + H+ + NH3		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate
PH: 7
Temperature: 25
uniprot:P24295 [glutamate dehydrogenase(Enzyme) mutant K89L/A163G]

H2O + L_Glutamate + NAD+ -> NH3 + H+ + 2_Oxoglutarate + NADH		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M Tris/HCl
PH: 8
Temperature: 25
uniprot:P24295 [glutamate dehydrogenase(Enzyme) mutant K89L]

2_Oxohexanoate + NH3 + H+ + NADH -> NAD+ + H2O + L_Norleucine		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate
PH: 7
Temperature: 25
uniprot:P24295 [glutamate dehydrogenase(Enzyme) mutant K89L/A163G]

NH3 + NADH + 2_Oxohexanoate + H+ -> H2O + L_Norleucine + NAD+		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate
PH: 7
Temperature: 25
uniprot:P24295 [glutamate dehydrogenase(Enzyme) mutant K89L/S380A/A163G]

NADH + NH3 + H+ + 2_Oxohexanoate -> NAD+ + H2O + L_Norleucine		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate
PH: 7
Temperature: 25
uniprot:P24295 [glutamate dehydrogenase(Enzyme) mutant K89L]

H2O + NAD+ + L_Methionine -> NH3 + 4_Methylthio_2_oxobutanoate + H+ + NADH		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M Tris/HCl
PH: 8
Temperature: 25
uniprot:P24295 [glutamate dehydrogenase(Enzyme) mutant K89L/A163G]

NAD+ + H2O + L_Methionine -> H+ + NH3 + NADH + 4_Methylthio_2_oxobutanoate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M Tris/HCl
PH: 8
Temperature: 25
uniprot:P24295 [glutamate dehydrogenase(Enzyme) mutant K89L]

L_Methionine + H2O + NAD+ -> NADH + H+ + 4_Methylthio_2_oxobutanoate + NH3		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate
PH: 7
Temperature: 25
uniprot:P24295 [glutamate dehydrogenase(Enzyme) mutant K89L/S380A/A163G]

NAD+ + L_Methionine + H2O -> NH3 + H+ + NADH + 4_Methylthio_2_oxobutanoate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate
PH: 7
Temperature: 25
uniprot:P24295 [glutamate dehydrogenase(Enzyme) mutant K89L/S380A/A163G]

L_Methionine + NAD+ + H2O -> H+ + NADH + 4_Methylthio_2_oxobutanoate + NH3		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M Tris/HCl
PH: 8
Temperature: 25
uniprot:P24295 [glutamate dehydrogenase(Enzyme) mutant K89L/A163G]

H2O + L_Methionine + NAD+ -> NADH + H+ + 4_Methylthio_2_oxobutanoate + NH3		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate
PH: 7
Temperature: 25
uniprot:P24295 [glutamate dehydrogenase(Enzyme) wildtype]

NAD+ + H2O + L_Glutamate -> 2_Oxoglutarate + NH3 + H+ + NADH		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate
PH: 7
Temperature: 25
uniprot:P24295 [glutamate dehydrogenase(Enzyme) mutant A163G]

H2O + NAD+ + L_Glutamate -> NH3 + H+ + NADH + 2_Oxoglutarate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate
PH: 7
Temperature: 25
uniprot:P24295 [glutamate dehydrogenase(Enzyme) mutant S380A]

NAD+ + H2O + L_Glutamate -> NH3 + H+ + NADH + 2_Oxoglutarate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate
PH: 7
Temperature: 25
uniprot:P24295 [glutamate dehydrogenase(Enzyme) mutant A163G]

H2O + L_Methionine + NAD+ -> NADH + H+ + NH3 + 4_Methylthio_2_oxobutanoate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M Tris/HCl
PH: 8
Temperature: 25
uniprot:P24295 [glutamate dehydrogenase(Enzyme) wildtype]

H2O + NAD+ + L_Norleucine -> NADH + NH3 + 2_Oxohexanoate + H+		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M Tris/HCl
PH: 8
Temperature: 25
uniprot:P24295 [glutamate dehydrogenase(Enzyme) mutant K89L]

H2O + NAD+ + L_Norleucine -> 2_Oxohexanoate + NH3 + H+ + NADH		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M Tris/HCl
PH: 8
Temperature: 25
uniprot:P24295 [glutamate dehydrogenase(Enzyme) mutant K89L/A163G]

H2O + L_Norleucine + NAD+ -> H+ + 2_Oxohexanoate + NH3 + NADH		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate
PH: 7
Temperature: 25
uniprot:P24295 [glutamate dehydrogenase(Enzyme) mutant K89L]

L_Norleucine + NAD+ + H2O -> 2_Oxohexanoate + NADH + H+ + NH3		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate
PH: 7
Temperature: 25
uniprot:P24295 [glutamate dehydrogenase(Enzyme) mutant K89L/A163G]

H2O + NAD+ + L_Norleucine -> NADH + 2_Oxohexanoate + H+ + NH3		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M Tris/HCl
PH: 8
Temperature: 25
uniprot:P24295 [glutamate dehydrogenase(Enzyme) mutant K89L/S380A/A163G]

NAD+ + H2O + L_Norleucine -> NADH + NH3 + H+ + 2_Oxohexanoate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M Tris/HCl
PH: 8
Temperature: 25
uniprot:P24295 [glutamate dehydrogenase(Enzyme) mutant K89L/S380A/A163G]

NAD+ + L_Norleucine + H2O -> H+ + NH3 + NADH + 2_Oxohexanoate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate
PH: 7
Temperature: 25
uniprot:P24295 [glutamate dehydrogenase(Enzyme) mutant A163G]

H2O + NAD+ + L_Norleucine -> NADH + NH3 + H+ + 2_Oxohexanoate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M Tris/HCl
PH: 8
Temperature: 25
uniprot:P24295 [glutamate dehydrogenase(Enzyme) mutant S380A]

H2O + L_Glutamate + NAD+ -> NH3 + NADH + H+ + 2_Oxoglutarate		 (( (( (kcat * E) ) * (A ^ n)) ) / (( (S_half ^ n) ) + (A ^ n))) buffer: 50 mM glycine/NaOH
PH: 9
Temperature: 25
uniprot:P24295 [glutamate dehydrogenase(Enzyme) mutant A163G]

L_Glutamate + NAD+ + H2O -> H+ + NADH + 2_Oxoglutarate + NH3		 (( (( (kcat * E) ) * (A ^ n)) ) / (( (S_half ^ n) ) + (A ^ n))) buffer: 50 mM glycine/NaOH
PH: 9
Temperature: 25
uniprot:P24295 [glutamate dehydrogenase(Enzyme) mutant K89L/A163G]

NAD+ + H2O + L_Glutamate -> 2_Oxoglutarate + NH3 + NADH + H+		 (( (( (kcat * E) ) * (A ^ n)) ) / (( (S_half ^ n) ) + (A ^ n))) buffer: 50 mM glycine/NaOH
PH: 9
Temperature: 25
uniprot:P24295 [glutamate dehydrogenase(Enzyme) wildtype]

NAD+ + H2O + L_Glutamate -> NH3 + NADH + H+ + 2_Oxoglutarate		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 0.1 M potassium phosphate
PH: 7
Temperature: 25
uniprot:P24295 [glutamate dehydrogenase(Enzyme) mutant A163G]

L_Glutamate + H2O + NAD+ -> H+ + 2_Oxoglutarate + NADH + NH3		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 0.1 M potassium phosphate
PH: 7
Temperature: 25
uniprot:P24295 [glutamate dehydrogenase(Enzyme) mutant S380A]

L_Glutamate + NAD+ + H2O -> 2_Oxoglutarate + H+ + NADH + NH3		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 0.1 M potassium phosphate
PH: 7
Temperature: 25
uniprot:P24295 [glutamate dehydrogenase(Enzyme) mutant K89L/S380A/A163G]

H+ + NH3 + NADH + 4_Methylthio_2_oxobutanoate -> NAD+ + H2O + L_Methionine		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate
PH: 7
Temperature: 25
uniprot:P24295 [glutamate dehydrogenase(Enzyme) mutant K89L]

NH3 + H+ + NADH + 4_Methylthio_2_oxobutanoate -> NAD+ + L_Methionine + H2O		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate
PH: 7
Temperature: 25
uniprot:P24295 [glutamate dehydrogenase(Enzyme) mutant K89L/A163G]

NADH + H+ + 4_Methylthio_2_oxobutanoate + NH3 -> H2O + L_Methionine + NAD+		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate
PH: 7
Temperature: 25
- [glutamate dehydrogenase(Enzyme) wildtype]

L_Glutamate + NAD+ + H2O -> NADH + NH3 + 2_Oxoglutarate + H+		 (( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 0.05 M Glycine
PH: 8.8
Temperature: 30
- [glutamate dehydrogenase(Enzyme) wildtype]

NADH + 2_Oxoglutarate + NH3 + H+ -> NAD+ + L_Glutamate + H2O		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM Phosphate
PH: 7.4
Temperature: 30
- [glutamate dehydrogenase(Enzyme) wildtype]

NH3 + 2_Oxoglutarate + H+ + NADH -> H2O + NAD+ + L_Glutamate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM Phosphate
PH: 7.4
Temperature: 30
- [glutamate dehydrogenase(Enzyme) wildtype]

NADH + NH3 + H+ + 2_Oxoglutarate -> L_Glutamate + H2O + NAD+		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM Phosphate
PH: 7.4
Temperature: 30
- [glutamate dehydrogenase(Enzyme) wildtype]

NAD+ + L_Glutamate + H2O -> NH3 + H+ + NADH + 2_Oxoglutarate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.05 M Glycine
PH: 8.8
Temperature: 30
- [glutamate dehydrogenase(Enzyme) wildtype]

H2O + L_Glutamate + NAD+ -> H+ + 2_Oxoglutarate + NH3 + NADH		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.05 M Glycine
PH: 8.8
Temperature: 30
uniprot:P39633 [glutamate dehydrogenase(Enzyme) wildtype]

H+ + NH3 + 2_Oxoglutarate + NADH -> L_Glutamate + NAD+ + H2O		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris
PH: 7.3
Temperature: 37
uniprot:P39633 [glutamate dehydrogenase(Enzyme) mutant E27F]

NADH + NH3 + 2_Oxoglutarate + H+ -> H2O + NAD+ + L_Glutamate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris
PH: 7.3
Temperature: 37
uniprot:P39633 [glutamate dehydrogenase(Enzyme) mutant Q144R]

H+ + 2_Oxoglutarate + NADH + NH3 -> H2O + NAD+ + L_Glutamate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris
PH: 7.3
Temperature: 37
uniprot:P39633 [glutamate dehydrogenase(Enzyme) wildtype]

NADH + NH3 + H+ + 2_Oxoglutarate -> NAD+ + L_Glutamate + H2O		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris
PH: 7.3
Temperature: 37
uniprot:P39633 [glutamate dehydrogenase(Enzyme) mutant E27F]

NADH + NH3 + 2_Oxoglutarate + H+ -> NAD+ + L_Glutamate + H2O		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris
PH: 7.3
Temperature: 37
uniprot:P39633 [glutamate dehydrogenase(Enzyme) wildtype]

2_Oxoglutarate + NADH + H+ + NH3 -> NAD+ + L_Glutamate + H2O		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris
PH: 7.3
Temperature: 37
uniprot:P39633 [glutamate dehydrogenase(Enzyme) mutant Q144R]

2_Oxoglutarate + H+ + NADH + NH3 -> NAD+ + H2O + L_Glutamate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris
PH: 7.3
Temperature: 37
uniprot:P39633 [glutamate dehydrogenase(Enzyme) mutant Q144R]

NH3 + 2_Oxoglutarate + H+ + NADH -> H2O + NAD+ + L_Glutamate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris
PH: 7.3
Temperature: 37
uniprot:P39633 [glutamate dehydrogenase(Enzyme) mutant E27F]

H+ + NADH + 2_Oxoglutarate + NH3 -> NAD+ + H2O + L_Glutamate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris
PH: 7.3
Temperature: 37
uniprot:P39633 [glutamate dehydrogenase(Enzyme) wildtype]

L_Glutamate + NAD+ + H2O -> NH3 + NADH + H+ + 2_Oxoglutarate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Potassium phosphate
PH: 7.7
Temperature: 37
uniprot:P39633 [glutamate dehydrogenase(Enzyme) wildtype]

NAD+ + H2O + L_Glutamate -> NH3 + H+ + 2_Oxoglutarate + NADH		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Potassium phosphate
PH: 7.7
Temperature: 37

Pathways

pathway id name
BioCyc:META_P162-PWY L-glutamate degradation V (via hydroxyglutarate)
BioCyc:META_GLUTAMATE-DEG1-PWY L-glutamate degradation I
BioCyc:YEAST_GLUTAMATE-DEG1-PWY glutamate degradation to 2-oxoglutarate
BioCyc:META_PWY-7126 ethylene biosynthesis IV (engineered)
BioCyc:MTBH37RV_GLUTAMATE-DEG1-PWY L-glutamate degradation I
PathBank:SMP0002303 Glutamate Metabolism