EC: 1.4.1.14

glutamate synthase (NADH) (L-glutamate:NAD+ oxidoreductase (transaminating))

enzyme lambda metabolic reaction experiment
uniprot:P39812 [glutamate synthase (NADH)(Enzyme) wildtype]

2_Oxoglutarate + L_Glutamine + H+ + NADH -> NAD+ + 2 L_Glutamate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 6.9
Temperature: 30
uniprot:P39812 [glutamate synthase (NADH)(Enzyme) wildtype]

H+ + NADH + 2_Oxoglutarate + L_Glutamine -> NAD+ + 2 L_Glutamate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 6.9
Temperature: 30
uniprot:P39812 [glutamate synthase (NADH)(Enzyme) wildtype]

NADH + H+ + 2_Oxoglutarate + L_Glutamine -> 2 L_Glutamate + NAD+		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 6.9
Temperature: 30
uniprot:P39812 [glutamate synthase (NADH)(Enzyme) wildtype]

2_Oxoglutarate + L_Glutamine + H+ + NADH -> NAD+ + 2 L_Glutamate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 6.9
Temperature: 30
uniprot:P39812 [glutamate synthase (NADH)(Enzyme) wildtype]

L_Glutamine + H+ + 2_Oxoglutarate + NADH -> 2 L_Glutamate + NAD+		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 6.9
Temperature: 30
uniprot:P39812 [glutamate synthase (NADH)(Enzyme) wildtype]

2_Oxoglutarate + NADH + L_Glutamine + H+ -> NAD+ + 2 L_Glutamate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 6.9
Temperature: 30
uniprot:Q12680 [glutamate synthase (NADH)(Enzyme) wildtype]

2_Oxoglutarate + NADH + L_Glutamine + H+ -> 2 L_Glutamate + NAD+		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM potassium phosphate
PH: 7.8
Temperature: 23
uniprot:Q12680 [glutamate synthase (NADH)(Enzyme) wildtype]

NADH + L_Glutamine + 2_Oxoglutarate + H+ -> 2 L_Glutamate + NAD+		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM potassium phosphate
PH: 7.8
Temperature: 23
uniprot:Q12680 [glutamate synthase (NADH)(Enzyme) wildtype]

L_Glutamine + H+ + 2_Oxoglutarate + NADH -> NAD+ + 2 L_Glutamate		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM potassium phosphate
PH: 7.8
Temperature: 23
uniprot:Q12680 [glutamate synthase (NADH)(Enzyme) wildtype]

L_Glutamine + 2_Oxoglutarate + NADH + H+ -> NAD+ + 2 L_Glutamate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate
PH: 7
Temperature: 22
uniprot:Q12680 [glutamate synthase (NADH)(Enzyme) wildtype]

NADH + L_Glutamine + 2_Oxoglutarate + H+ -> NAD+ + 2 L_Glutamate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate
PH: 7
Temperature: 22
uniprot:Q12680 [glutamate synthase (NADH)(Enzyme) wildtype]

L_Glutamine + NADH + H+ + 2_Oxoglutarate -> NAD+ + 2 L_Glutamate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate
PH: 7
Temperature: 22

Pathways

pathway id name
BioCyc:META_PWY-5505 L-glutamate and L-glutamine biosynthesis
BioCyc:YEAST_PWY-6963 ammonia assimilation cycle I
BioCyc:META_PWY-6963 ammonia assimilation cycle I
BioCyc:YEAST_GLUGLNSYN-PWY glutamate biosynthesis from glutamine
BioCyc:META_GLUGLNSYN-PWY L-glutamate biosynthesis IV
BioCyc:ARA_PWY-6963 ammonia assimilation cycle I
BioCyc:ARA_GLUGLNSYN-PWY L-glutamate biosynthesis IV
Plant Reactome:R-OSA-1119443 Glutamate synthase cycle
Plant Reactome:R-OSA-2744345 Metabolism and regulation
Plant Reactome:R-OSA-5368291 Inorganic nutrients metabolism
PlantCyc:ARA_PWY-6963 ammonia assimilation cycle I
PlantCyc:ARA_GLUGLNSYN-PWY L-glutamate biosynthesis IV
PlantCyc:ARA_PWY-3282 superpathway of ammonia assimilation (plants)
PathBank:SMP0002403 Nitrogen Metabolism
PathBank:SMP0002303 Glutamate Metabolism
PathBank:SMP0002427 Glutamic Acid Metabolism