EC: 1.3.1.48

13,14-dehydro-15-oxoprostaglandin 13-reductase (11alpha-hydroxy-9,15-dioxoprostanoate:NAD(P)+ Delta13-oxidoreductase)

enzyme lambda metabolic reaction experiment
uniprot:Q8VDQ1 [15-oxoprostaglandin 13-oxidase(Enzyme) wildtype]

(5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + NADPH + H+ -> NADP+ + (5Z)_(15S)_11alpha_Hydroxy_9,15_dioxoprostanoate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 mM Tris-HCl
PH: 7.4
Temperature: 37
uniprot:Q8VDQ1 [15-oxoprostaglandin 13-oxidase(Enzyme) wildtype]

H+ + NADPH + 15_Keto_prostaglandin_E1 -> 13,14_Dihydro_15_keto_prostaglandin_E1 + NADP+		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 mM Tris-HCl
PH: 7.4
Temperature: 37
uniprot:Q8VDQ1 [15-oxoprostaglandin 13-oxidase(Enzyme) wildtype]

H+ + NADPH + (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate -> NADP+ + (5Z)_(15S)_11alpha_Hydroxy_9,15_dioxoprostanoate		 (( (Vmax * B) ) / (Km + B)) buffer: 0.1 mM Tris-HCl
PH: 7.4
Temperature: 37
uniprot:Q8VDQ1 [15-oxoprostaglandin 13-oxidase(Enzyme) wildtype]

NADPH + H+ + 15_Keto_prostaglandin_F2alpha -> NADP+ + 13,14_Dihydro_15_keto_prostaglandin_F2alpha		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 mM Tris-HCl
PH: 7.4
Temperature: 37
uniprot:Q8VDQ1 [15-oxoprostaglandin 13-oxidase(Enzyme) wildtype]

H+ + NADPH + 15_Keto_prostaglandin_F1alpha -> 13,14_Dihydro_15_keto_prostaglandin_F1alpha + NADP+		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 mM Tris-HCl
PH: 7.4
Temperature: 37
uniprot:Q8N8N7 [15-oxoprostaglandin 13-oxidase(Enzyme) mutant Y259F Trx-tag, His6-tag]

NADPH + H+ + (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate -> (5Z)_(15S)_11alpha_Hydroxy_9,15_dioxoprostanoate + NADP+		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Tris-HCl, 1 mM EDTA, 1 mM DTT
PH: 7.5
Temperature: 37
uniprot:Q8N8N7 [15-oxoprostaglandin 13-oxidase(Enzyme) mutant Y259F Trx-tag, His6-tag]

H+ + NADPH + (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate -> (5Z)_(15S)_11alpha_Hydroxy_9,15_dioxoprostanoate + NADP+		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Tris-HCl, 1 mM EDTA, 1 mM DTT
PH: 7.5
Temperature: 37
uniprot:Q8N8N7 [15-oxoprostaglandin 13-oxidase(Enzyme) mutant Y64F Trx-tag, His6-tag]

NADPH + H+ + (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate -> NADP+ + (5Z)_(15S)_11alpha_Hydroxy_9,15_dioxoprostanoate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Tris-HCl, 1 mM EDTA, 1 mM DTT
PH: 7.5
Temperature: 37
uniprot:Q8N8N7 [15-oxoprostaglandin 13-oxidase(Enzyme) mutant Y64F Trx-tag, His6-tag]

NADPH + (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + H+ -> (5Z)_(15S)_11alpha_Hydroxy_9,15_dioxoprostanoate + NADP+		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Tris-HCl, 1 mM EDTA, 1 mM DTT
PH: 7.5
Temperature: 37
uniprot:Q8N8N7 [15-oxoprostaglandin 13-oxidase(Enzyme) wildtype Trx-tag, His6-tag]

(5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + NADPH + H+ -> NADP+ + (5Z)_(15S)_11alpha_Hydroxy_9,15_dioxoprostanoate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Tris-HCl, 1 mM EDTA, 1 mM DTT
PH: 7.5
Temperature: 37
uniprot:Q8N8N7 [15-oxoprostaglandin 13-oxidase(Enzyme) wildtype Trx-tag, His6-tag]

H+ + (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + NADPH -> NADP+ + (5Z)_(15S)_11alpha_Hydroxy_9,15_dioxoprostanoate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Tris-HCl, 1 mM EDTA, 1 mM DTT
PH: 7.5
Temperature: 37

Pathways

pathway id name
BioCyc:HUMAN_PWY3DJ-11470 sphingosine and sphingosine-1-phosphate metabolism
BioCyc:META_PWY3DJ-11470 sphingosine and sphingosine-1-phosphate metabolism
BioCyc:MOUSE_PWY3DJ-11470 sphingosine and sphingosine-1-phosphate metabolism
Reactome:R-HSA-2142691 Synthesis of Leukotrienes (LT) and Eoxins (EX)
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-HSA-8978868 Fatty acid metabolism
Reactome:R-HSA-2162123 Synthesis of Prostaglandins (PG) and Thromboxanes (TX)
Reactome:R-HSA-9018678 Biosynthesis of specialized proresolving mediators (SPMs)
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-2142753 Arachidonate metabolism
Reactome:R-HSA-2142700 Biosynthesis of Lipoxins (LX)