EC: 1.3.1.3

DELTA4-3-oxosteroid 5beta-reductase (5beta-cholestan-3-one:NADP+ 4,5-oxidoreductase)

enzyme lambda metabolic reaction experiment
uniprot:P51857 [Delta4-3-oxosteroid 5beta-reductase(Enzyme) mutant Y132F]

Androst_4_ene_3,17_dione + NADPH + H+ -> NADP+ + 5beta_Androstane_3,17_dione		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4
PH: 7.3
Temperature: 37
uniprot:P51857 [Delta4-3-oxosteroid 5beta-reductase(Enzyme) wildtype]

NADPH + Androst_4_ene_3,17_dione + H+ -> 5beta_Androstane_3,17_dione + NADP+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4
PH: 7.3
Temperature: 37
uniprot:P51857 [Delta4-3-oxosteroid 5beta-reductase(Enzyme) mutant V309F]

H+ + NADPH + Androst_4_ene_3,17_dione -> NADP+ + 5beta_Androstane_3,17_dione		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4
PH: 7.3
Temperature: 37
uniprot:P51857 [Delta4-3-oxosteroid 5beta-reductase(Enzyme) wildtype]

H+ + Testosterone + NADPH -> NADP+ + 5beta_Androstane_17beta_ol_3_one		 (( (Vmax * S) ) / (( (Km + S) ) + (( (S ^ 2) ) / Ki))) buffer: 100 mM potassium phosphate, 4% acetonitrile
PH: 6
Temperature: 37
uniprot:P31210 [Delta4-3-oxosteroid 5beta-reductase(Enzyme) wildtype]

NADPH + H+ + Cortisone -> 17alpha,21_Dihydroxy_5beta_pregnane_3,11,20_trione + NADP+		 (( (Vmax * A) ) / (Km + A)) buffer: 10 mM Acetate
PH: 5.6
Temperature: 37
uniprot:P31210 [Delta4-3-oxosteroid 5beta-reductase(Enzyme) wildtype]

NADPH + Cortisone + H+ -> NADP+ + 17alpha,21_Dihydroxy_5beta_pregnane_3,11,20_trione		 (( (Vmax * A) ) / (Km + A)) buffer: 10 mM Acetate
PH: 5.6
Temperature: 37
uniprot:P31210 [Delta4-3-oxosteroid 5beta-reductase(Enzyme) wildtype]

H+ + NADPH + Cortisone -> 17alpha,21_Dihydroxy_5beta_pregnane_3,11,20_trione + NADP+		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl
PH: 7.4
Temperature: 37
uniprot:P31210 [Delta4-3-oxosteroid 5beta-reductase(Enzyme) wildtype]

NADPH + Androst_4_ene_3,17_dione + H+ -> NADP+ + 5beta_Androstane_3,17_dione		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl
PH: 7.4
Temperature: 37
uniprot:P31210 [Delta4-3-oxosteroid 5beta-reductase(Enzyme) wildtype]

H+ + Cortisol + NADPH -> NADP+ + 11beta,17alpha,21_Trihydroxy_5beta_pregnane_3,20_dione		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl
PH: 7.4
Temperature: 25
uniprot:P31210 [Delta4-3-oxosteroid 5beta-reductase(Enzyme) wildtype]

NADPH + H+ + 7alpha,12alpha_Dihydroxycholest_4_en_3_one -> 7alpha,12alpha_Dihydroxy_5beta_cholestan_3_one + NADP+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl
PH: 7.4
Temperature: 25
uniprot:P31210 [Delta4-3-oxosteroid 5beta-reductase(Enzyme) wildtype]

H+ + NADPH + 7alpha_Hydroxycholest_4_en_3_one -> 7alpha_Hydroxy_5beta_cholestan_3_one + NADP+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl
PH: 7.4
Temperature: 25
uniprot:P31210 [Delta4-3-oxosteroid 5beta-reductase(Enzyme) wildtype]

NADPH + Cortisone + H+ -> 17alpha,21_Dihydroxy_5beta_pregnane_3,11,20_trione + NADP+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl
PH: 7.4
Temperature: 25
uniprot:P31210 [Delta4-3-oxosteroid 5beta-reductase(Enzyme) wildtype]

H+ + Progesterone + NADPH -> NADP+ + 5beta_Pregnane_3,20_dione		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl
PH: 7.4
Temperature: 25
uniprot:P31210 [Delta4-3-oxosteroid 5beta-reductase(Enzyme) wildtype]

NADPH + 7alpha_Hydroxycholest_4_en_3_one + H+ -> NADP+ + 7alpha_Hydroxy_5beta_cholestan_3_one		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl
PH: 7.4
Temperature: 25
uniprot:P31210 [Delta4-3-oxosteroid 5beta-reductase(Enzyme) wildtype]

Androst_4_ene_3,17_dione + H+ + NADPH -> NADP+ + 5beta_Androstane_3,17_dione		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl
PH: 7.4
Temperature: 25
uniprot:P31210 [Delta4-3-oxosteroid 5beta-reductase(Enzyme) wildtype]

NADPH + H+ + Testosterone -> NADP+ + 5beta_Androstane_17beta_ol_3_one		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl
PH: 7.4
Temperature: 25

Pathways

pathway id name
BioCyc:META_PWY-6032 cardenolide biosynthesis
PlantCyc:PLANT_PWY-6032 cardenolide biosynthesis
Reactome:R-HSA-194068 Bile acid and bile salt metabolism
Reactome:R-HSA-193807 Synthesis of bile acids and bile salts via 27-hydroxycholesterol
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-556833 Metabolism of lipids
Reactome:R-MMU-8957322 Metabolism of steroids
Reactome:R-MMU-193775 Synthesis of bile acids and bile salts via 24-hydroxycholesterol
Reactome:R-RNO-193368 Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-HSA-193775 Synthesis of bile acids and bile salts via 24-hydroxycholesterol
Reactome:R-MMU-193368 Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol
Reactome:R-RNO-192105 Synthesis of bile acids and bile salts
Reactome:R-HSA-192105 Synthesis of bile acids and bile salts
Reactome:R-MMU-194068 Bile acid and bile salt metabolism
Reactome:R-MMU-193807 Synthesis of bile acids and bile salts via 27-hydroxycholesterol
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-556833 Metabolism of lipids
Reactome:R-RNO-8957322 Metabolism of steroids
Reactome:R-RNO-193775 Synthesis of bile acids and bile salts via 24-hydroxycholesterol
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-8957322 Metabolism of steroids
Reactome:R-HSA-193368 Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol
Reactome:R-MMU-192105 Synthesis of bile acids and bile salts
Reactome:R-RNO-194068 Bile acid and bile salt metabolism
Reactome:R-RNO-193807 Synthesis of bile acids and bile salts via 27-hydroxycholesterol
WikiPathways:WP5280 Glucocorticoid biosynthesis
PathBank:SMP0120658 17-beta Hydroxysteroid Dehydrogenase III Deficiency
PathBank:SMP0120690 Congenital Lipoid Adrenal Hyperplasia (CLAH) or Lipoid CAH
PathBank:SMP0120858 11-beta-Hydroxylase Deficiency (CYP11B1)
PathBank:SMP0030406 Androstenedione Metabolism
PathBank:SMP0000068 Androgen and Estrogen Metabolism
PathBank:SMP0000373 Adrenal Hyperplasia Type 3 or Congenital Adrenal Hyperplasia Due to 21-Hydroxylase Deficiency
PathBank:SMP0000318 Congenital Bile Acid Synthesis Defect Type III
PathBank:SMP0000576 21-Hydroxylase Deficiency (CYP21)
PathBank:SMP0000718 3-beta-Hydroxysteroid Dehydrogenase Deficiency
PathBank:SMP0120697 Familial Hypercholanemia (FHCA)
PathBank:SMP0120849 17-alpha-Hydroxylase Deficiency (CYP17)
PathBank:SMP0120861 Corticosterone Methyl Oxidase II Deficiency (CMO II)
PathBank:SMP0120869 3-beta-Hydroxysteroid Dehydrogenase Deficiency
PathBank:SMP0000035 Bile Acid Biosynthesis
PathBank:SMP0000356 17-beta Hydroxysteroid Dehydrogenase III Deficiency
PathBank:SMP0000314 Congenital Bile Acid Synthesis Defect Type II
PathBank:SMP0000315 Cerebrotendinous Xanthomatosis (CTX)
PathBank:SMP0000575 11-beta-Hydroxylase Deficiency (CYP11B1)
PathBank:SMP0000717 Apparent Mineralocorticoid Excess Syndrome
PathBank:SMP0087329 Bile Acid Biosynthesis
PathBank:SMP0120672 Adrenal Hyperplasia Type 5 or Congenital Adrenal Hyperplasia Due to 17 alpha-Hydroxylase Deficiency
PathBank:SMP0120688 Congenital Bile Acid Synthesis Defect Type III
PathBank:SMP0120848 Aromatase Deficiency
PathBank:SMP0120860 Corticosterone Methyl Oxidase I Deficiency (CMO I)
PathBank:SMP0120868 Apparent Mineralocorticoid Excess Syndrome
PathBank:SMP0000130 Steroidogenesis
PathBank:SMP0000371 Congenital Lipoid Adrenal Hyperplasia (CLAH) or Lipoid CAH
PathBank:SMP0000317 Familial Hypercholanemia (FHCA)
PathBank:SMP0000565 Aromatase Deficiency
PathBank:SMP0000577 Corticosterone Methyl Oxidase I Deficiency (CMO I)
PathBank:SMP0087344 Androgen and Estrogen Metabolism
PathBank:SMP0087364 Androstenedione Metabolism
PathBank:SMP0120671 Adrenal Hyperplasia Type 3 or Congenital Adrenal Hyperplasia Due to 21-Hydroxylase Deficiency
PathBank:SMP0120683 Cerebrotendinous Xanthomatosis (CTX)
PathBank:SMP0120687 Congenital Bile Acid Synthesis Defect Type II
PathBank:SMP0120715 Zellweger Syndrome
PathBank:SMP0120859 21-Hydroxylase Deficiency (CYP21)
PathBank:SMP0120871 27-Hydroxylase Deficiency
PathBank:SMP0000372 Adrenal Hyperplasia Type 5 or Congenital Adrenal Hyperplasia Due to 17 alpha-Hydroxylase Deficiency
PathBank:SMP0000316 Zellweger Syndrome
PathBank:SMP0000566 17-alpha-Hydroxylase Deficiency (CYP17)
PathBank:SMP0000578 Corticosterone Methyl Oxidase II Deficiency (CMO II)
PathBank:SMP0000720 27-Hydroxylase Deficiency
PathBank:SMP0087331 Steroidogenesis