EC: 1.2.1.24

succinate-semialdehyde dehydrogenase (NAD+) (succinate-semialdehyde:NAD+ oxidoreductase)

enzyme lambda metabolic reaction experiment
- [succinate-semialdehyde dehydrogenase (NAD+)(Enzyme) wildtype]

Acetaldehyde + H2O + NAD+ -> Acetate + NADH + H+		 (( (Vmax * B) ) / (Km + B)) buffer: 0.1 M Sodium pyrophosphate, 5 mM EDTA
PH: 9
Temperature: 25
- [succinate-semialdehyde dehydrogenase (NAD+)(Enzyme) wildtype]

NAD+ + H2O + Succinate_semialdehyde -> H+ + NADH + Succinate		 (( (Vmax * B) ) / (Km + B)) buffer: 0.1 M Sodium pyrophosphate, 5 mM EDTA
PH: 9
Temperature: 25
- [succinate-semialdehyde dehydrogenase (NAD+)(Enzyme) wildtype]

Succinate_semialdehyde + NAD+ + H2O -> Succinate + NADH + H+		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Sodium pyrophosphate, 5 mM EDTA
PH: 9
Temperature: 25
- [succinate-semialdehyde dehydrogenase (NAD+)(Enzyme) wildtype]

H2O + Propanal + NAD+ -> Propanoate + H+ + NADH		 (( (Vmax * B) ) / (Km + B)) buffer: 0.1 M Sodium pyrophosphate, 5 mM EDTA
PH: 9
Temperature: 25
uniprot:P51649 [succinate-semialdehyde dehydrogenase (NAD+)(Enzyme) wildtype CM isoform]

NAD+ + H2O + Succinate_semialdehyde -> NADH + Succinate + H+		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Phosphate, 50 mM KCl, 0.1 mM EDTA, 20 mM 2-Mercaptoethanol
PH: 7.2
Temperature: 37
uniprot:P51649 [succinate-semialdehyde dehydrogenase (NAD+)(Enzyme) wildtype CM isoform]

H2O + NAD+ + Succinate_semialdehyde -> H+ + NADH + Succinate		 (( (Vmax * B) ) / (Km + B)) buffer: 100 mM Phosphate, 50 mM KCl, 0.1 mM EDTA, 20 mM 2-Mercaptoethanol
PH: 7.2
Temperature: 37
uniprot:P51649 [succinate-semialdehyde dehydrogenase (NAD+)(Enzyme) wildtype DE isoform]

NAD+ + Succinate_semialdehyde + H2O -> NADH + Succinate + H+		 (( (Vmax * B) ) / (Km + B)) buffer: 100 mM Phosphate, 50 mM KCl, 0.1 mM EDTA, 20 mM 2-Mercaptoethanol
PH: 7.2
Temperature: 37
uniprot:P51649 [succinate-semialdehyde dehydrogenase (NAD+)(Enzyme) wildtype DE isoform]

H2O + NAD+ + Succinate_semialdehyde -> NADH + Succinate + H+		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Phosphate, 50 mM KCl, 0.1 mM EDTA, 20 mM 2-Mercaptoethanol
PH: 7.2
Temperature: 37
uniprot:Q6F9G0 [succinate-semialdehyde dehydrogenase (NAD+)(Enzyme) wildtype TgnE His-tagged]

NAD+ + Succinate_semialdehyde + H2O -> Succinate + H+ + NADH		 (( (( (E * kcat) ) * S) ) / (Km * S)) buffer: 50 mM Tris-HCl
PH: 8
Temperature: 25
uniprot:Q6F9G0 [succinate-semialdehyde dehydrogenase (NAD+)(Enzyme) wildtype TgnE His-tagged]

H2O + Succinate_semialdehyde + NAD+ -> Succinate + H+ + NADH		 (( (( (E * kcat) ) * S) ) / (Km * S)) buffer: 50 mM Tris-HCl
PH: 8
Temperature: 25

Pathways

pathway id name
BioCyc:HUMAN_PWY-6535 4-aminobutyrate degradation
BioCyc:META_PWY-6535 4-aminobutanoate degradation I
BioCyc:HUMAN_GLUDEG-I-PWY GABA shunt
BioCyc:META_GLUDEG-I-PWY GABA shunt
BioCyc:MOUSE_PWY-6535 4-aminobutyrate degradation I
BioCyc:ARA_PWY-6535 4-aminobutanoate degradation I
BioCyc:ARA_PWY-4321 L-glutamate degradation IV
BioCyc:MOUSE_GLUDEG-I-PWY glutamate degradation III (via 4-aminobutyrate)
BioCyc:ARA_PWY-6473 4-aminobutanoate degradation IV
BioCyc:MOUSE_PWY-6473 4-aminobutyrate degradation IV
BioCyc:MOUSE_PWY-4321 glutamate degradation IV
Plant Reactome:R-OSA-1119458 GABA shunt
Plant Reactome:R-OSA-2744345 Metabolism and regulation
Plant Reactome:R-OSA-2744343 Amino acid metabolism
Plant Reactome:R-OSA-5655124 Amino acid catabolism
PlantCyc:ARA_PWY-6473 4-aminobutanoate degradation IV
PlantCyc:ARA_PWY-4321 L-glutamate degradation IV
PlantCyc:PLANT_PWY-6473 4-aminobutanoate degradation IV
PlantCyc:ARA_PWY-6535 4-aminobutanoate degradation I
PlantCyc:PLANT_PWY-4321 L-glutamate degradation IV
Reactome:R-HSA-112315 Transmission across Chemical Synapses
Reactome:R-HSA-888590 GABA synthesis, release, reuptake and degradation
Reactome:R-MMU-916853 Degradation of GABA
Reactome:R-RNO-112316 Neuronal System
Reactome:R-HSA-916853 Degradation of GABA
Reactome:R-MMU-112310 Neurotransmitter release cycle
Reactome:R-RNO-112315 Transmission across Chemical Synapses
Reactome:R-RNO-888590 GABA synthesis, release, reuptake and degradation
Reactome:R-HSA-112316 Neuronal System
Reactome:R-MMU-112315 Transmission across Chemical Synapses
Reactome:R-MMU-888590 GABA synthesis, release, reuptake and degradation
Reactome:R-RNO-112310 Neurotransmitter release cycle
Reactome:R-HSA-112310 Neurotransmitter release cycle
Reactome:R-MMU-112316 Neuronal System
Reactome:R-RNO-916853 Degradation of GABA
WikiPathways:WP4157 GABA metabolism (aka GHB)
PathBank:SMP0120484 Glutaric Aciduria Type I
PathBank:SMP0120593 Hyperlysinemia II or Saccharopinuria
PathBank:SMP0000339 Hyperinsulinism-Hyperammonemia Syndrome
PathBank:SMP0063600 Betaine Metabolism
PathBank:SMP0120592 Hyperlysinemia I, Familial
PathBank:SMP0000385 Homocarnosinosis
PathBank:SMP0000136 2-Hydroxyglutric Aciduria (D and L Form)
PathBank:SMP0000567 Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0108762 Sarcosine Oncometabolite Pathway
PathBank:SMP0120651 2-Aminoadipic 2-Oxoadipic Aciduria
PathBank:SMP0120526 Saccharopinuria/Hyperlysinemia II
PathBank:SMP0120635 Pyridoxine Dependency with Seizures
PathBank:SMP0000072 Glutamate Metabolism
PathBank:SMP0000243 4-Hydroxybutyric Aciduria/Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0002427 Glutamic Acid Metabolism
PathBank:SMP0012467 Butanoate Metabolism
PathBank:SMP0063605 Carnitine Synthesis
PathBank:SMP0063635 Lysine Degradation