EC: 1.14.11.15

gibberellin 3beta-dioxygenase ((gibberellin-20),2-oxoglutarate:oxygen oxidoreductase (3beta-hydroxylating))

enzyme lambda metabolic reaction experiment
uniprot:Q39103 [Fe2+]

O2 + 2_Oxoglutarate + Gibberellin_A20 -> Gibberellin_A1 + Succinate + CO2		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 2 mM NADPH, 2 mg/ml BSA, 2 mM DTT
PH: 7.5
Temperature: 20
uniprot:Q39103 [Fe2+]

Gibberellin_A9 + O2 + 2_Oxoglutarate -> Gibberellin_A4 + CO2 + Succinate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 2 mM NADPH, 2 mg/ml BSA, 2 mM DTT
PH: 7.5
Temperature: 20
uniprot:Q3I411 [gibberellin 3beta-dioxygenase(Enzyme) wildtype]

Gibberellin_A20 + O2 + 2_Oxoglutarate -> CO2 + Gibberellin_A1 + Succinate		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 36
uniprot:Q3I411 [gibberellin 3beta-dioxygenase(Enzyme) wildtype]

Gibberellin_A9 + 2_Oxoglutarate + O2 -> CO2 + Gibberellin_A4 + Succinate		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 36
uniprot:O24648 [Fe2+]

2_Oxoglutarate + O2 + Gibberellin_A20 -> CO2 + Gibberellin_A1 + Succinate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Tris-HCl, 2 mg/ml BSA, 1 mg/ml catalase, 4 mM DTT
PH: 7.5
Temperature: 30
uniprot:O24648 [Fe2+]

2_Oxoglutarate + O2 + Gibberellin_A9 -> CO2 + Gibberellin_A4 + Succinate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Tris-HCl, 2 mg/ml BSA, 1 mg/ml catalase, 4 mM DTT
PH: 7.5
Temperature: 30
uniprot:O24648 [Fe2+]

2_Oxoglutarate + Gibberellin_A9 + O2 -> CO2 + Gibberellin_A4 + Succinate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Tris-HCl, 2 mg/ml BSA, 1 mg/ml catalase, 4 mM DTT
PH: 7.5
Temperature: 30
uniprot:Q9C971 [Fe2+]

O2 + 2_Oxoglutarate + Gibberellin_A20 -> Gibberellin_A1 + CO2 + Succinate		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 4 mM ascorbate, 4 mM DTT, 4 mM 2-oxoglutarate, 2 mg/ml BSA, 1 mg/ml catalase
PH: 7.9
Temperature: 30
uniprot:Q9ZT84 [Fe2+]

O2 + Gibberellin_A20 + 2_Oxoglutarate -> Gibberellin_A1 + Succinate + CO2		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM Tris-HCl
PH: 7.5
Temperature: 30
uniprot:Q9ZT84 [Fe2+]

Gibberellin_A9 + O2 + 2_Oxoglutarate -> CO2 + Gibberellin_A4 + Succinate		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM Tris-HCl
PH: 7.5
Temperature: 30

Pathways

pathway id name
BioCyc:META_PWY-7232 gibberellin biosynthesis V
BioCyc:ARA_PWY-5035 gibberellin biosynthesis III (early C-13 hydroxylation)
BioCyc:ARA_PWY-5070 gibberellin biosynthesis I (non C-3, non C-13 hydroxylation)
BioCyc:META_PWY-5070 gibberellin biosynthesis I (non C-3, non C-13 hydroxylation)
BioCyc:META_PWY-5052 superpathway of gibberellin biosynthesis
BioCyc:ARA_PWY-5036 gibberellin biosynthesis II (early C-3 hydroxylation)
BioCyc:META_PWY-5035 gibberellin biosynthesis III (early C-13 hydroxylation)
BioCyc:META_PWY-5036 gibberellin biosynthesis II (early C-3 hydroxylation)
PlantCyc:ARA_PWY-5052 superpathway of gibberellin biosynthesis
PlantCyc:PLANT_PWY-7232 gibberellin biosynthesis V
PlantCyc:PLANT_PWY-5052 superpathway of gibberellin biosynthesis
PlantCyc:PLANT_PWY-5036 gibberellin biosynthesis II (early C-3 hydroxylation)
PlantCyc:PLANT_PWY-5070 gibberellin biosynthesis I (non C-3, non C-13 hydroxylation)
PlantCyc:ARA_PWY-5070 gibberellin biosynthesis I (non C-3, non C-13 hydroxylation)
PlantCyc:BREADWHEAT_PWY-5035 gibberellin biosynthesis III (early C-13 hydroxylation)
PlantCyc:ARA_PWY-5036 gibberellin biosynthesis II (early C-3 hydroxylation)
PlantCyc:BREADWHEAT_PWY-5052 superpathway of gibberellin biosynthesis
PlantCyc:BREADWHEAT_PWY-5070 gibberellin biosynthesis I (non C-3, non C-13 hydroxylation)
PlantCyc:BREADWHEAT_PWY-7232 gibberellin biosynthesis V
PlantCyc:PLANT_PWY-5035 gibberellin biosynthesis III (early C-13 hydroxylation)
PlantCyc:ARA_PWY-5035 gibberellin biosynthesis III (early C-13 hydroxylation)
PathBank:SMP0012069 Gibberellin Biosynthesis I (Early C-3 Hydroxylation)
PathBank:SMP0012070 Gibberellin Biosynthesis II (Early C-13 Hydroxylation)
PathBank:SMP0012071 Gibberellin Biosynthesis III (Non C-3, Non C-13 Hydroxylation)