EC: 1.1.2.3

L-lactate dehydrogenase (cytochrome) ((S)-lactate:ferricytochrome-c 2-oxidoreductase)

enzyme lambda metabolic reaction experiment
uniprot:P00175 [L-lactate dehydrogenase (cytochrome)(Enzyme) wildtype]

L_Lactate + 2 Hexacyanoferrate(III) -> 2 Hexacyanoferrate(II) + Pyruvate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M Na+/K+ phosphate, 1 mM EDTA
PH: 7
Temperature: 5
uniprot:P00175 [L-lactate dehydrogenase (cytochrome)(Enzyme) wildtype]

2 Ferricytochrome_c + L_Lactate -> Pyruvate + 2 Ferrocytochrome_c		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M Na+/K+ phosphate, 1 mM EDTA
PH: 7
Temperature: 5
uniprot:P00175 [L-lactate dehydrogenase (cytochrome)(Enzyme) wildtype]

L_Lactate + 2 Ferricytochrome_c -> 2 Ferrocytochrome_c + Pyruvate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M Na+/K+ phosphate, 1 mM EDTA
PH: 7
Temperature: 5
uniprot:P00175 [L-lactate dehydrogenase (cytochrome)(Enzyme) wildtype]

L_Lactate + 2 Hexacyanoferrate(III) -> 2 Hexacyanoferrate(II) + Pyruvate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M Na+/K+ phosphate, 1 mM EDTA
PH: 7
Temperature: 5
uniprot:P00175 [L-lactate dehydrogenase (cytochrome)(Enzyme) wildtype]

2 Hexacyanoferrate(III) + L_Lactate -> 2 Hexacyanoferrate(II) + Pyruvate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M phosphate
PH: 7
Temperature: 30
uniprot:P00175 [L-lactate dehydrogenase (cytochrome)(Enzyme) wildtype]

L_Lactate + 2 Hexacyanoferrate(III) -> 2 Hexacyanoferrate(II) + Pyruvate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M phosphate, 500 mM acetate
PH: 7
Temperature: 5
uniprot:P00175 [L-lactate dehydrogenase (cytochrome)(Enzyme) wildtype]

L_Lactate + 2 Hexacyanoferrate(III) -> Pyruvate + 2 Hexacyanoferrate(II)		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M phosphate
PH: 7
Temperature: 5
uniprot:P00175 [L-lactate dehydrogenase (cytochrome)(Enzyme) mutant Y143F]

2 Hexacyanoferrate(III) + L_Lactate -> 2 Hexacyanoferrate(II) + Pyruvate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M phosphate
PH: 7
Temperature: 5
uniprot:P00175 [L-lactate dehydrogenase (cytochrome)(Enzyme) mutant Y143F]

2 Hexacyanoferrate(III) + L_Lactate -> Pyruvate + 2 Hexacyanoferrate(II)		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M phosphate
PH: 7
Temperature: 30
uniprot:P00175 [L-lactate dehydrogenase (cytochrome)(Enzyme) mutant Y143F]

2 Hexacyanoferrate(III) + L_Lactate -> 2 Hexacyanoferrate(II) + Pyruvate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M phosphate, 500 mM acetate
PH: 7
Temperature: 5
uniprot:P00175 [L-lactate dehydrogenase (cytochrome)(Enzyme) wildtype]

2 Ferricytochrome_c + L_Lactate -> Pyruvate + 2 Ferrocytochrome_c		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M phosphate
PH: 7
Temperature: 30
uniprot:P00175 [L-lactate dehydrogenase (cytochrome)(Enzyme) wildtype]

2 Ferricytochrome_c + L_Lactate -> Pyruvate + 2 Ferrocytochrome_c		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M phosphate
PH: 7
Temperature: 5
uniprot:P00175 [L-lactate dehydrogenase (cytochrome)(Enzyme) wildtype]

L_Lactate + 2 Ferricytochrome_c -> Pyruvate + 2 Ferrocytochrome_c		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M phosphate, 500 mM acetate
PH: 7
Temperature: 5
uniprot:P00175 [L-lactate dehydrogenase (cytochrome)(Enzyme) mutant Y143F]

L_Lactate + 2 Ferricytochrome_c -> 2 Ferrocytochrome_c + Pyruvate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M phosphate
PH: 7
Temperature: 30
uniprot:P00175 [L-lactate dehydrogenase (cytochrome)(Enzyme) wildtype]

2 Ferricytochrome_c + L_Lactate -> Pyruvate + 2 Ferrocytochrome_c		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.043 M phosphate
PH: 7
Temperature: 30
uniprot:P00175 [L-lactate dehydrogenase (cytochrome)(Enzyme) mutant Y143F]

2 Ferricytochrome_c + L_Lactate -> 2 Ferrocytochrome_c + Pyruvate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M phosphate
PH: 7
Temperature: 5
uniprot:P00175 [L-lactate dehydrogenase (cytochrome)(Enzyme) mutant Y143F]

L_Lactate + 2 Ferricytochrome_c -> Pyruvate + 2 Ferrocytochrome_c		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M phosphate, 50 mM acetate
PH: 7
Temperature: 5
uniprot:P00175 [L-lactate dehydrogenase (cytochrome)(Enzyme) wildtype]

2 Hexacyanoferrate(III) + L_Lactate -> 2 Hexacyanoferrate(II) + Pyruvate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.043 M phosphate
PH: 7
Temperature: 30
uniprot:P00175 [L-lactate dehydrogenase (cytochrome)(Enzyme) mutant Y143F]

2 Hexacyanoferrate(III) + L_Lactate -> 2 Hexacyanoferrate(II) + Pyruvate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.043 M phosphate
PH: 7
Temperature: 30
uniprot:P00175 [L-lactate dehydrogenase (cytochrome)(Enzyme) wildtype]

L_Lactate + 2 Ferricytochrome_c -> Pyruvate + 2 Ferrocytochrome_c		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 43 mM phosphate
PH: 7
Temperature: 30
uniprot:P00175 [L-lactate dehydrogenase (cytochrome)(Enzyme) wildtype]

2 Ferricytochrome_c + L_Lactate -> 2 Ferrocytochrome_c + Pyruvate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 43 mM phosphate
PH: 7
Temperature: 30
uniprot:P00175 [L-lactate dehydrogenase (cytochrome)(Enzyme) mutant Y143F]

L_Lactate + 2 Ferricytochrome_c -> Pyruvate + 2 Ferrocytochrome_c		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 43 mM phosphate
PH: 7
Temperature: 30
uniprot:P00175 [L-lactate dehydrogenase (cytochrome)(Enzyme) mutant Y143F]

2 Ferricytochrome_c + L_Lactate -> Pyruvate + 2 Ferrocytochrome_c		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 43 mM phosphate
PH: 7
Temperature: 30
uniprot:P00175 [L-lactate dehydrogenase (cytochrome)(Enzyme) wildtype]

2 Hexacyanoferrate(III) + L_Lactate -> Pyruvate + 2 Hexacyanoferrate(II)		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 43 mM phosphate
PH: 7
Temperature: 30
uniprot:P00175 [L-lactate dehydrogenase (cytochrome)(Enzyme) wildtype]

2 Hexacyanoferrate(III) + L_Lactate -> 2 Hexacyanoferrate(II) + Pyruvate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 43 mM phosphate
PH: 7
Temperature: 30
uniprot:P00175 [L-lactate dehydrogenase (cytochrome)(Enzyme) mutant Y143F]

L_Lactate + 2 Hexacyanoferrate(III) -> Pyruvate + 2 Hexacyanoferrate(II)		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 43 mM phosphate
PH: 7
Temperature: 30
uniprot:P00175 [L-lactate dehydrogenase (cytochrome)(Enzyme) mutant Y143F]

2 Hexacyanoferrate(III) + L_Lactate -> Pyruvate + 2 Hexacyanoferrate(II)		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 43 mM phosphate
PH: 7
Temperature: 30
uniprot:P00175 [L-lactate dehydrogenase (cytochrome)(Enzyme) mutant Y143F]

L_Lactate + 2 Ferricytochrome_c -> Pyruvate + 2 Ferrocytochrome_c		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.043 M phosphate
PH: 7
Temperature: 30
uniprot:P00175 [L-lactate dehydrogenase (cytochrome)(Enzyme) wildtype]

L_Lactate + 2 Hexacyanoferrate(III) -> Pyruvate + 2 Hexacyanoferrate(II)		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM phosphate
PH: 7
Temperature: 30
uniprot:P00175 [L-lactate dehydrogenase (cytochrome)(Enzyme) mutant Y143F]

2 Hexacyanoferrate(III) + L_Lactate -> Pyruvate + 2 Hexacyanoferrate(II)		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM phosphate
PH: 7
Temperature: 30
uniprot:P00175 [L-lactate dehydrogenase (cytochrome)(Enzyme) mutant Y143F]

L_Lactate + 2 Hexacyanoferrate(III) -> Pyruvate + 2 Hexacyanoferrate(II)		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M Na+/K+ phosphate, 1 mM EDTA
PH: 7
Temperature: 5
uniprot:P00175 [L-lactate dehydrogenase (cytochrome)(Enzyme) mutant Y143]

L_Lactate + 2 Hexacyanoferrate(III) -> 2 Hexacyanoferrate(II) + Pyruvate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M Na+/K+ phosphate, 1 mM EDTA
PH: 7
Temperature: 5
uniprot:P00175 [L-lactate dehydrogenase (cytochrome)(Enzyme) wildtype]

L_Lactate + 2 Ferricytochrome_c -> Pyruvate + 2 Ferrocytochrome_c		 (( (( (E * kcat) ) * A) ) / (( (Km + A) ) + (( (A ^ 2) ) / Ki))) buffer: 0.1 M Na+/K+ phosphate, 1 mM EDTA
PH: 7
Temperature: 5
uniprot:P00175 [L-lactate dehydrogenase (cytochrome)(Enzyme) wildtype]

L_Lactate + 2 Ferricytochrome_c -> Pyruvate + 2 Ferrocytochrome_c		 (( (( (E * kcat) ) * A) ) / (( (Km + A) ) + (( (A ^ 2) ) / Ki))) buffer: 0.1 M Na+/K+ phosphate, 1 mM EDTA
PH: 7
Temperature: 5
uniprot:P00175 [L-lactate dehydrogenase (cytochrome)(Enzyme) mutant Y143F]

2 Ferricytochrome_c + L_Lactate -> Pyruvate + 2 Ferrocytochrome_c		 (( (( (E * kcat) ) * A) ) / (( (Km + A) ) + (( (A ^ 2) ) / Ki))) buffer: 0.1 M Na+/K+ phosphate, 1 mM EDTA
PH: 7
Temperature: 5
uniprot:P00175 [L-lactate dehydrogenase (cytochrome)(Enzyme) wildtype]

2 Hexacyanoferrate(III) + L_Lactate -> 2 Hexacyanoferrate(II) + Pyruvate		 (( (( (E * kcat) ) * A) ) / (( (Km + A) ) + (( (A ^ 2) ) / Ki))) buffer: 0.1 M Na+/K+ phosphate, 1 mM EDTA,
PH: 7
Temperature: 5
uniprot:P00175 [L-lactate dehydrogenase (cytochrome)(Enzyme) mutant Y143F]

L_Lactate + 2 Hexacyanoferrate(III) -> Pyruvate + 2 Hexacyanoferrate(II)		 (( (( (E * kcat) ) * A) ) / (( (Km + A) ) + (( (A ^ 2) ) / Ki))) buffer: 0.1 M Na+/K+ phosphate, 1 mM EDTA
PH: 7
Temperature: 5
uniprot:P00175 [L-lactate dehydrogenase (cytochrome)(Enzyme) wildtype]

L_Lactate + 2 Ferricytochrome_c -> Pyruvate + 2 Ferrocytochrome_c		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM phosphate
PH: 7
Temperature: 30
uniprot:P00175 [L-lactate dehydrogenase (cytochrome)(Enzyme) wildtype]

L_Lactate + 2 Ferricytochrome_c -> 2 Ferrocytochrome_c + Pyruvate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM phosphate
PH: 7
Temperature: 30
uniprot:P00175 [L-lactate dehydrogenase (cytochrome)(Enzyme) mutant Y143F]

2 Ferricytochrome_c + L_Lactate -> Pyruvate + 2 Ferrocytochrome_c		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM phosphate
PH: 7
Temperature: 30
uniprot:P00175 [L-lactate dehydrogenase (cytochrome)(Enzyme) mutant Y143F]

2 Ferricytochrome_c + L_Lactate -> 2 Ferrocytochrome_c + Pyruvate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM phosphate
PH: 7
Temperature: 30
uniprot:P00175 [L-lactate dehydrogenase (cytochrome)(Enzyme) mutant Y143F]

L_Lactate + 2 Hexacyanoferrate(III) -> Pyruvate + 2 Hexacyanoferrate(II)		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM phosphate
PH: 7
Temperature: 30

Pathways

pathway id name
BioCyc:META_PWY-5458 methylglyoxal degradation V
BioCyc:YEAST_PWY-5458 methylglyoxal degradation V