EC: 1.1.1.40

malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) ((S)-malate:NADP+ oxidoreductase (oxaloacetate-decarboxylating))

enzyme lambda metabolic reaction experiment
uniprot:P48163 [malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)(Enzyme) wildtype isoenzym ME1]

NADP+ + L_Malate -> NADPH + Pyruvate + CO2 + H+		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 3 mM MnCl2
PH: 7.4
Temperature: 37
uniprot:P48163 [malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)(Enzyme) wildtype isoenzym ME1]

NADP+ + L_Malate -> H+ + Pyruvate + CO2 + NADPH		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 3 mM MnCl2
PH: 7.4
Temperature: 37
uniprot:P48163 [malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)(Enzyme) wildtype isoenzym ME1]

NADP+ + L_Malate -> H+ + NADPH + CO2 + Pyruvate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 3 mM MnCl2
PH: 7.4
Temperature: 37
uniprot:P48163 [malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)(Enzyme) wildtype isoenzym ME1]

NADP+ + L_Malate -> Pyruvate + H+ + NADPH + CO2		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 3 mM MnCl2
PH: 7.4
Temperature: 37
uniprot:P48163 [malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)(Enzyme) wildtype isoenzym ME1]

NADP+ + L_Malate -> CO2 + H+ + Pyruvate + NADPH		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 3 mM MnCl2
PH: 7.4
Temperature: 37
uniprot:P48163 [malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)(Enzyme) wildtype isoenzym ME1]

L_Malate + NADP+ -> H+ + Pyruvate + NADPH + CO2		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 3 mM MnCl2
PH: 7.4
Temperature: 37
- [malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)(Enzyme) wildtype chimeric LR His-tagged]

NADP+ + L_Malate -> CO2 + Pyruvate + NADPH + H+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tricine-Mops
PH: 7
Temperature: 36
- [malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)(Enzyme) wildtype chimeric LR His-tagged]

NADP+ + L_Malate -> Pyruvate + CO2 + H+ + NADPH		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl
PH: 8
Temperature: 36
- [malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)(Enzyme) wildtype chimeric LR His-tagged]

NADP+ + L_Malate -> Pyruvate + NADPH + H+ + CO2		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl
PH: 8
Temperature: 36
- [malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)(Enzyme) wildtype chimeric RL His-tagged]

L_Malate + NADP+ -> H+ + CO2 + NADPH + Pyruvate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl
PH: 8
Temperature: 36
- [malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)(Enzyme) wildtype chimeric LpR His-tagged]

NADP+ + L_Malate -> Pyruvate + CO2 + H+ + NADPH		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl
PH: 8
Temperature: 36
- [malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)(Enzyme) wildtype chimeric RpL His-tagged]

L_Malate + NADP+ -> Pyruvate + NADPH + H+ + CO2		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl
PH: 8
Temperature: 36
- [malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)(Enzyme) wildtype chimeric LRL His-tagged]

NADP+ + L_Malate -> NADPH + Pyruvate + H+ + CO2		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl
PH: 8
Temperature: 36
- [malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)(Enzyme) wildtype chimeric LpR His-tagged]

L_Malate + NADP+ -> CO2 + H+ + NADPH + Pyruvate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl
PH: 8
Temperature: 36
- [malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)(Enzyme) wildtype chimeric RL His-tagged]

L_Malate + NADP+ -> NADPH + CO2 + Pyruvate + H+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl
PH: 8
Temperature: 36
- [malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)(Enzyme) wildtype chimeric RpL His-tagged]

NADP+ + L_Malate -> H+ + Pyruvate + NADPH + CO2		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl
PH: 8
Temperature: 36
- [malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)(Enzyme) wildtype chimeric LRL His-tagged]

L_Malate + NADP+ -> NADPH + CO2 + Pyruvate + H+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl
PH: 8
Temperature: 36
- [malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)(Enzyme) wildtype chimeric RLR His-tagged]

L_Malate + NADP+ -> NADPH + Pyruvate + CO2 + H+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl
PH: 8
Temperature: 36
- [malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)(Enzyme) wildtype chimeric RL His-tagged]

L_Malate + NADP+ -> Pyruvate + CO2 + NADPH + H+		 (( (Vmax * (( (S / Kc) ) + (( (phi * (S ^ 2)) ) / (Kc * Kr)))) ) / (( (( (1 + (S / Kc)) ) + (S / Kr)) ) + (( (S ^ 2) ) / (Kc * Kr)))) buffer: 50 mM Tricine-Mops
PH: 7
Temperature: 36
- [malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)(Enzyme) wildtype chimeric RpL His-tagged]

L_Malate + NADP+ -> H+ + CO2 + Pyruvate + NADPH		 (( (Vmax * (( (S / Kc) ) + (( (phi * (S ^ 2)) ) / (Kc * Kr)))) ) / (( (( (1 + (S / Kc)) ) + (S / Kr)) ) + (( (S ^ 2) ) / (Kc * Kr)))) buffer: 50 mM Tricine-Mops
PH: 7
Temperature: 36
- [malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)(Enzyme) wildtype chimeric LRL His-tagged]

L_Malate + NADP+ -> Pyruvate + CO2 + NADPH + H+		 (( (Vmax * (( (S / Kc) ) + (( (phi * (S ^ 2)) ) / (Kc * Kr)))) ) / (( (( (1 + (S / Kc)) ) + (S / Kr)) ) + (( (S ^ 2) ) / (Kc * Kr)))) buffer: 50 mM Tricine-Mops
PH: 7
Temperature: 36
- [malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)(Enzyme) wildtype chimeric LpR His-tagged]

L_Malate + NADP+ -> CO2 + H+ + Pyruvate + NADPH		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tricine-Mops
PH: 7
Temperature: 36
- [malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)(Enzyme) wildtype chimeric RLR His-tagged]

NADP+ + L_Malate -> CO2 + H+ + NADPH + Pyruvate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tricine-Mops
PH: 7
Temperature: 36
- [malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)(Enzyme) wildtype chimeric RLR His-tagged]

NADP+ + L_Malate -> Pyruvate + CO2 + NADPH + H+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl
PH: 8
Temperature: 36
- [Mg2+]

L_Malate + NADP+ -> Pyruvate + CO2 + NADPH + H+		 (Vmax / (( (1 + (Km / S)) ) + (S / Ki))) buffer: 25 mM Tricine-Mops-NaOH
PH: 7
Temperature: 30
- [Mg2+]

NADP+ + L_Malate -> CO2 + H+ + Pyruvate + NADPH		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM Tricine-Mops-NaOH
PH: 8
Temperature: 30
- [Mg2+]

NADP+ + L_Malate -> NADPH + CO2 + H+ + Pyruvate		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM Tricine-Mops-NaOH
PH: 7
Temperature: 30
- [Mg2+]

NADP+ + L_Malate -> NADPH + Pyruvate + H+ + CO2		 (Vmax / (( (1 + (Km / S)) ) + (S / Ki))) buffer: 25 mM Tricine-Mops-NaOH
PH: 7
Temperature: 30
- [Mg2+]

NADP+ + L_Malate -> CO2 + NADPH + H+ + Pyruvate		 (Vmax / (( (1 + (Km / S)) ) + (S / Ki))) buffer: 25 mM Tricine-Mops-NaOH
PH: 7
Temperature: 30
- [Mg2+]

NADP+ + L_Malate -> NADPH + Pyruvate + H+ + CO2		 (Vmax / (( (1 + (Km / S)) ) + (S / Ki))) buffer: 25 mM Tricine-Mops-NaOH
PH: 7
Temperature: 30
- [Mg2+]

L_Malate + NADP+ -> CO2 + H+ + NADPH + Pyruvate		 (Vmax / (( (1 + (Km / S)) ) + (S / Ki))) buffer: 25 mM Tricine-Mops-NaOH
PH: 7
Temperature: 30
uniprot:Q9LYG3 [malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)(Enzyme) mutant isoform NADP-ME2-del2 (44 amino acids truncated)]

NADP+ + L_Malate -> Pyruvate + CO2 + H+ + NADPH		 (( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Tris-HCl, 10 mM MgCl2
PH: 7.5
Temperature: 30
uniprot:Q9LYG3 [malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)(Enzyme) mutant isoform NADP-ME2-del2 (44 amino acids truncated)]

NADP+ + L_Malate -> NADPH + H+ + Pyruvate + CO2		 (( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Tris-HCl, 10 mM MgCl2
PH: 7.5
Temperature: 30
uniprot:P40927 [Mn2+]

NADP+ + L_Malate -> Pyruvate + NADPH + H+ + CO2		 (( (( (( (kcat * E) ) * A) ) * Co) ) / (( (( (( (Kd_Co * Km_A) ) + (Km_Co * A)) ) + (Km_A * Co)) ) + (Co * A))) buffer: 66.7 mM triethanolamine-HCl
PH: 7.4
Temperature: 30
uniprot:P40927 [Mn2+]

L_Malate + NADP+ -> Pyruvate + CO2 + H+ + NADPH		 (( (( (( (kcat * E) ) * A) ) * Co) ) / (( (( (( (Kd_Co * Km_A) ) + (Km_Co * A)) ) + (Km_A * Co)) ) + (Co * A))) buffer: 66.7 mM triethanolamine-HCl
PH: 7.4
Temperature: 30
uniprot:P40927 [Mn2+]

L_Malate + NADP+ -> Pyruvate + H+ + NADPH + CO2		 (( (( (( (kcat * E) ) * A) ) * Co) ) / (( (( (( (Kd_Co * Km_A) ) + (Km_Co * A)) ) + (Km_A * Co)) ) + (Co * A))) buffer: 66.7 mM triethanolamine-HCl
PH: 7.4
Temperature: 30
uniprot:P40927 [Mn2+]

L_Malate + NADP+ -> NADPH + H+ + Pyruvate + CO2		 (( (( (( (kcat * E) ) * A) ) * Co) ) / (( (( (( (Kd_Co * Km_A) ) + (Km_Co * A)) ) + (Km_A * Co)) ) + (Co * A))) buffer: 66.7 mM triethanolamine-HCl
PH: 7.4
Temperature: 30
uniprot:P40927 [Mn2+]

L_Malate + NADP+ -> Pyruvate + NADPH + CO2 + H+		 (( (( (( (kcat * E) ) * A) ) * Co) ) / (( (( (( (Kd_Co * Km_A) ) + (Km_Co * A)) ) + (Km_A * Co)) ) + (Co * A))) buffer: 66.7 mM triethanolamine-HCl
PH: 7.4
Temperature: 30
uniprot:P40927 [Mn2+]

NADP+ + L_Malate -> Pyruvate + NADPH + H+ + CO2		 (( (( (( (kcat * E) ) * A) ) * Co) ) / (( (( (( (Kd_Co * Km_A) ) + (Km_Co * A)) ) + (Km_A * Co)) ) + (Co * A))) buffer: 66.7 mM triethanolamine-HCl
PH: 7.4
Temperature: 30
uniprot:P40927 [Mn2+]

L_Malate + NADP+ -> H+ + Pyruvate + CO2 + NADPH		 (( (( (( (kcat * E) ) * A) ) * Co) ) / (( (( (( (Kd_Co * Km_A) ) + (Km_Co * A)) ) + (Km_A * Co)) ) + (Co * A))) buffer: 66.7 mM triethanolamine-HCl
PH: 7.4
Temperature: 30
uniprot:P40927 [Mn2+]

NADP+ + L_Malate -> CO2 + NADPH + H+ + Pyruvate		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 66.7 mM triethanolamine-HCl
PH: 7.4
Temperature: 30
uniprot:P40927 [Mn2+]

NADP+ + L_Malate -> Pyruvate + CO2 + NADPH + H+		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 66.7 mM triethanolamine-HCl
PH: 7.4
Temperature: 30
uniprot:P48163 [Mn2+]

L_Malate + NAD+ -> Pyruvate + NADH + H+ + CO2		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM Hepes/KOH, 0.5 mM dithiothreitol, 1 mM EDTA/Na4, 0.1% bovine serum albumin
PH: 7.5
Temperature: 30
uniprot:P48163 [Mn2+]

NADP+ + L_Malate -> Pyruvate + CO2 + NADPH + H+		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM Hepes/KOH, 0.5 mM dithiothreitol, 1 mM EDTA/Na4, 0.1% bovine serum albumin
PH: 7.5
Temperature: 30
uniprot:P48163 [Mn2+]

NADP+ + L_Malate -> CO2 + NADPH + H+ + Pyruvate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Hepes/KOH, 0.5 mM dithiothreitol, 1 mM EDTA/Na4, 0.1% bovine serum albumin
PH: 7.5
Temperature: 30
uniprot:P48163 [Mg2+]

L_Malate + NADP+ -> H+ + CO2 + NADPH + Pyruvate		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM Hepes/KOH, 0.5 mM dithiothreitol, 1 mM EDTA/Na4, 0.1% bovine serum albumin
PH: 7.5
Temperature: 30
uniprot:P48163 [Mg2+]

NADP+ + L_Malate -> CO2 + H+ + NADPH + Pyruvate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Hepes/KOH, 0.5 mM dithiothreitol, 1 mM EDTA/Na4, 0.1% bovine serum albumin
PH: 7.5
Temperature: 30
uniprot:P48163 [Mn2+]

Pyruvate + CO2 + NADPH -> NADP+ + L_Malate		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM Hepes/KOH, 0.5 mM dithiothreitol, 1 mM EDTA/Na4, 0.1% bovine serum albumin
PH: 7.5
Temperature: 30
uniprot:P48163 [Mn2+]

NADPH + CO2 + Pyruvate -> L_Malate + NADP+		 (( (Vmax * C) ) / (Km + C)) buffer: 50 mM Hepes/KOH, 0.5 mM dithiothreitol, 1 mM EDTA/Na4, 0.1% bovine serum albumin
PH: 7.5
Temperature: 30
uniprot:P48163 [Mn2+]

Pyruvate + NADPH + CO2 -> L_Malate + NADP+		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Hepes/KOH, 0.5 mM dithiothreitol, 1 mM EDTA/Na4, 0.1% bovine serum albumin
PH: 7.5
Temperature: 30
uniprot:P40927 [Mn2+]

L_Malate + NADP+ -> Pyruvate + H+ + NADPH + CO2		 (( (( (( (kcat * E) ) * A) ) * Co) ) / (( (( (( (Kd_Co * Km_A) ) + (Km_Co * A)) ) + (Km_A * Co)) ) + (Co * A))) buffer: 66.7 mM triethanolamine-HCl
PH: 7.4
Temperature: 30
uniprot:P40927 [Mn2+]

L_Malate + NADP+ -> NADPH + Pyruvate + H+ + CO2		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 66.7 mM triethanolamine-HCl
PH: 7.4
Temperature: 30
uniprot:P40927 [Mn2+]

NADP+ + L_Malate -> H+ + CO2 + Pyruvate + NADPH		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 66.7 mM triethanolamine-HCl
PH: 7.4
Temperature: 30
uniprot:P40927 [Mn2+]

L_Malate + NADP+ -> H+ + NADPH + CO2 + Pyruvate		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 66.7 mM triethanolamine-HCl
PH: 7.4
Temperature: 30
uniprot:P40927 [Mn2+]

NADP+ + L_Malate -> CO2 + H+ + NADPH + Pyruvate		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 66.7 mM triethanolamine-HCl
PH: 7.4
Temperature: 30
uniprot:P40927 [Mn2+]

NADP+ + L_Malate -> H+ + NADPH + CO2 + Pyruvate		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 66.7 mM triethanolamine-HCl
PH: 7.4
Temperature: 30
uniprot:P40927 [Mn2+]

L_Malate + NADP+ -> CO2 + H+ + NADPH + Pyruvate		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 66.7 mM triethanolamine-HCl
PH: 7.4
Temperature: 30
uniprot:O82191 [malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)(Enzyme) wildtype isoform NADP-ME1]

NADP+ + L_Malate -> H+ + NADPH + CO2 + Pyruvate		 (( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Tris-HCl, 10 mM MgCl2
PH: 7.5
Temperature: 36
uniprot:Q9M4Q9 [Mg2+]

L_Malate + NADP+ -> Pyruvate + H+ + NADPH + CO2		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Hepes-KOH
PH: 7.65
Temperature: 25
uniprot:P16243 [AMP]

L_Malate + NADP+ -> CO2 + H+ + NADPH + Pyruvate		 (( (( (E * kcat) ) * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) buffer: 50 mM Tris-HCl
PH: 8
Temperature: 30
uniprot:P16243 [AMP]

NADP+ + L_Malate -> H+ + Pyruvate + NADPH + CO2		 (( (( (E * kcat) ) * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) buffer: 50 mM Tris-HCl
PH: 8
Temperature: 30
uniprot:P16243 [Adenosine 2'-phosphate]

NADP+ + L_Malate -> Pyruvate + NADPH + CO2 + H+		 (( (( (E * kcat) ) * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) buffer: 50 mM Tris-HCl
PH: 8
Temperature: 30
uniprot:P16243 [Adenosine 2'-phosphate]

L_Malate + NADP+ -> Pyruvate + CO2 + H+ + NADPH		 (( (( (E * kcat) ) * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) buffer: 50 mM Tris-HCl
PH: 8
Temperature: 30
uniprot:P16243 [Mg2+]

L_Malate + NAD+ -> Pyruvate + H+ + CO2 + NADH		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl
PH: 7
Temperature: 30
uniprot:Q9M4Q9 [Mg2+]

L_Malate + NADP+ -> NADPH + H+ + Pyruvate + CO2		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM Hepes-KOH
PH: 7.65
Temperature: 25
uniprot:P16243 [Mg2+]

L_Malate + NAD+ -> H+ + CO2 + Pyruvate + NADH		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl
PH: 7
Temperature: 30
uniprot:P16243 [Mg2+]

L_Malate + NADP+ -> H+ + CO2 + Pyruvate + NADPH		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl
PH: 8
Temperature: 30
uniprot:P16243 [Mg2+]

NADP+ + L_Malate -> Pyruvate + NADPH + CO2 + H+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl
PH: 8
Temperature: 30
uniprot:P16243 [Mg2+]

L_Malate + NADP+ -> H+ + CO2 + Pyruvate + NADPH		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl
PH: 8
Temperature: 30
uniprot:P16243 [Mg2+]

L_Malate + NADP+ -> H+ + CO2 + NADPH + Pyruvate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl
PH: 8
Temperature: 30
uniprot:P16243 [Mg2+]

L_Malate + NADP+ -> Pyruvate + CO2 + H+ + NADPH		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl
PH: 8
Temperature: 30
uniprot:P16243 [Mg2+]

L_Malate + NADP+ -> NADPH + Pyruvate + H+ + CO2		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl
PH: 8
Temperature: 30
uniprot:P40927 [Mg2+]

NADP+ + L_Malate -> NADPH + H+ + CO2 + Pyruvate		 (( (V * B) ) / (( (Kb * (1 + (I / Kis))) ) + (B * (1 + (I / Kii))))) buffer: 33 mM triethanolamine (HCl), 0.07 mg/ml crystalline bovine albumin
PH: 7
Temperature: 28
uniprot:P40927 [Mg2+]

L_Malate + NADP+ -> H+ + CO2 + NADPH + Pyruvate		 (( (V * A) ) / (( (Ka * (1 + (I / Kis))) ) + (A * (1 + (I / Kii))))) buffer: 33 mM triethanolamine (HCl), 0.07 mg/ml crystalline bovine albumin
PH: 7
Temperature: 28
uniprot:P40927 [Mg2+]

NADP+ + L_Malate -> NADPH + Pyruvate + CO2 + H+		 (( (V * B) ) / (Kb + (B * (1 + (I / Kii))))) buffer: 33 mM triethanolamine (HCl), 0.07 mg/ml crystalline bovine albumin
PH: 7
Temperature: 28
uniprot:P40927 [Mg2+]

NADP+ + L_Malate -> Pyruvate + H+ + CO2 + NADPH		 (( (V * A) ) / (Ka + (A * (1 + (I / Kii))))) buffer: 33 mM triethanolamine (HCl), 0.07 mg/ml crystalline bovine albumin
PH: 7
Temperature: 28
uniprot:P40927 [Mg2+]

NADP+ + L_Malate -> H+ + CO2 + Pyruvate + NADPH		 (( (( (V * A) ) * B) ) / (( (( (( (Kia * Kb) ) + (Ka * B)) ) + (Kb * A)) ) + (A * B))) buffer: 33 mM triethanolamine (HCl), 0.07 mg/ml crystalline bovine albumin
PH: 7
Temperature: 28
uniprot:P40927 [Mg2+]

L_Malate + NADP+ -> CO2 + H+ + Pyruvate + NADPH		 (( (V * B) ) / (( (Kb * (1 + (I / Kis))) ) + (B * (1 + (I / Kii))))) buffer: 33 mM triethanolamine (HCl), 0.07 mg/ml crystalline bovine albumin
PH: 7
Temperature: 28
uniprot:P40927 [Mg2+]

L_Malate + NADP+ -> Pyruvate + NADPH + H+ + CO2		 (( (V * B) ) / (( (Kb * (1 + (I / Kis))) ) + (B * (1 + (I / Kii))))) buffer: 33 mM triethanolamine (HCl), 0.07 mg/ml crystalline bovine albumin
PH: 7
Temperature: 28
uniprot:P40927 [Mg2+]

L_Malate + NADP+ -> Pyruvate + H+ + CO2 + NADPH		 (( (V * A) ) / (( (Ka * (1 + (I / Kis))) ) + A)) buffer: 33 mM triethanolamine (HCl), 0.07 mg/ml crystalline bovine albumin
PH: 7
Temperature: 28
uniprot:Q16798 [Mn2+]

L_Malate + NADP+ -> Pyruvate + NADPH + CO2 + H+		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM MOPS, 50 mM (N-[2-Hydroxyethyl]-piperazine-N'-[3-propanesulfonic acid])
PH: 7.8
Temperature: 37
uniprot:P48163 [Mn2+]

NADP+ + L_Malate -> H+ + NADPH + Pyruvate + CO2		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM MOPS, 50 mM (N-[2-Hydroxyethyl]-piperazine-N'-[3-propanesulfonic acid])
PH: 7.8
Temperature: 37
uniprot:P48163 [Mn2+]

NADP+ + L_Malate -> Pyruvate + CO2 + H+ + NADPH		 (( (Vmax * C) ) / (Km + C)) buffer: 50 mM MOPS, 50 mM (N-[2-Hydroxyethyl]-piperazine-N'-[3-propanesulfonic acid])
PH: 7.8
Temperature: 37
uniprot:Q16798 [Mg2+]

NADP+ + L_Malate -> Pyruvate + CO2 + H+ + NADPH		 (( (Vmax * C) ) / (Km + C)) buffer: 50 mM MOPS, 50 mM (N-[2-Hydroxyethyl]-piperazine-N'-[3-propanesulfonic acid])
PH: 7.8
Temperature: 37
uniprot:P48163 [Mg2+]

L_Malate + NADP+ -> NADPH + H+ + CO2 + Pyruvate		 (( (Vmax * C) ) / (Km + C)) buffer: 50 mM MOPS, 50 mM (N-[2-Hydroxyethyl]-piperazine-N'-[3-propanesulfonic acid])
PH: 7.8
Temperature: 37
uniprot:Q16798 [Mn2+]

NAD+ + L_Malate -> CO2 + H+ + Pyruvate + NADH		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM MOPS, 50 mM (N-[2-Hydroxyethyl]-piperazine-N'-[3-propanesulfonic acid])
PH: 7.4
Temperature: 37
uniprot:P48163 [Mn2+]

NAD+ + L_Malate -> Pyruvate + H+ + CO2 + NADH		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM MOPS, 50 mM (N-[2-Hydroxyethyl]-piperazine-N'-[3-propanesulfonic acid])
PH: 6.5
Temperature: 37
uniprot:P48163 [Mn2+]

Pyruvate + NADPH + CO2 + H+ -> L_Malate + NADP+		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM MOPS, 50 mM (N-[2-Hydroxyethyl]-piperazine-N'-[3-propanesulfonic acid])
PH: 7.2
Temperature: 37
uniprot:Q16798 [Mn2+]

L_Malate + NADP+ -> Pyruvate + CO2 + H+ + NADPH		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM MOPS, 50 mM (N-[2-Hydroxyethyl]-piperazine-N'-[3-propanesulfonic acid])
PH: 7.8
Temperature: 37
uniprot:P48163 [Mn2+]

NADP+ + L_Malate -> CO2 + Pyruvate + H+ + NADPH		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM MOPS, 50 mM (N-[2-Hydroxyethyl]-piperazine-N'-[3-propanesulfonic acid])
PH: 7.8
Temperature: 37
uniprot:Q16798 [Mg2+]

NADP+ + L_Malate -> H+ + NADPH + CO2 + Pyruvate		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM MOPS, 50 mM (N-[2-Hydroxyethyl]-piperazine-N'-[3-propanesulfonic acid])
PH: 7.8
Temperature: 37
uniprot:P48163 [Mg2+]

L_Malate + NADP+ -> Pyruvate + NADPH + CO2 + H+		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM MOPS, 50 mM (N-[2-Hydroxyethyl]-piperazine-N'-[3-propanesulfonic acid])
PH: 7.8
Temperature: 37
uniprot:P48163 [malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)(Enzyme) wildtype isoenzyme ME1]

NAD+ + L_Malate -> Pyruvate + CO2 + NADH + H+		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM Tris-HCl, 20 mM Imidazole-HCl, 1 mM MnCl2
PH: 6.4
Temperature: 37
uniprot:P48163 [malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)(Enzyme) wildtype isoenzyme ME1]

L_Malate + NAD+ -> CO2 + Pyruvate + H+ + NADH		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 20 mM Imidazole-HCl, 1 mM MnCl2
PH: 6.4
Temperature: 37
uniprot:P48163 [malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)(Enzyme) wildtype isoenzyme ME1]

H+ + CO2 + NADPH + Pyruvate -> NADP+ + L_Malate		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM Tris-HCl, 20 mM Imidazole-HCl, 1 mM MnCl2
PH: 7.2
Temperature: 37
uniprot:P48163 [malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)(Enzyme) wildtype isoenzyme ME1]

NADPH + CO2 + H+ + Pyruvate -> L_Malate + NADP+		 (( (Vmax * C) ) / (Km + C)) buffer: 50 mM Tris-HCl, 20 mM Imidazole-HCl, 1 mM MnCl2
PH: 7.2
Temperature: 37
uniprot:P48163 [malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)(Enzyme) wildtype isoenzyme ME1]

H+ + Pyruvate + NADPH + CO2 -> L_Malate + NADP+		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 20 mM Imidazole-HCl, 1 mM MnCl2
PH: 7.2
Temperature: 37
uniprot:P48163 [malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)(Enzyme) wildtype isoenzyme ME1]

NADP+ + L_Malate -> H+ + NADPH + Pyruvate + CO2		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM Tris-HCl, 1 mM MnCl2
PH: 7.8
Temperature: 37
uniprot:P48163 [malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)(Enzyme) wildtype isoenzyme ME1]

NADP+ + L_Malate -> Pyruvate + H+ + NADPH + CO2		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 1 mM MnCl2
PH: 7.8
Temperature: 37
uniprot:P48163 [Mn2+]

NADPH + Pyruvate + CO2 + H+ -> NADP+ + L_Malate		 (( (Vmax * C) ) / (Km + C)) buffer: 50 mM MOPS, 50 mM (N-[2-Hydroxyethyl]-piperazine-N'-[3-propanesulfonic acid])
PH: 7.2
Temperature: 37
uniprot:P48163 [Mn2+]

H+ + CO2 + NADPH + Pyruvate -> L_Malate + NADP+		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM MOPS, 50 mM (N-[2-Hydroxyethyl]-piperazine-N'-[3-propanesulfonic acid])
PH: 7.2
Temperature: 37
uniprot:Q16798 [Mn2+]

L_Malate + NADP+ -> Pyruvate + NADPH + H+ + CO2		 (( (Vmax * C) ) / (Km + C)) buffer: 50 mM MOPS, 50 mM (N-[2-Hydroxyethyl]-piperazine-N'-[3-propanesulfonic acid])
PH: 7.8
Temperature: 37
uniprot:P76558 [Mg2+]

L_Malate + NADP+ -> NADPH + CO2 + H+ + Pyruvate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl
PH: 7.5
Temperature: 30
uniprot:D3ZJH9 [Mg2+]

NADP+ + L_Malate -> NADPH + H+ + CO2 + Pyruvate		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM MOPS, 120 mM KCl, 1 mM EGTA, 0.02% Triton X-100
PH: 7.2
Temperature: 37
uniprot:D3ZJH9 [Mg2+]

L_Malate + NADP+ -> NADPH + H+ + Pyruvate + CO2		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM MOPS, 120 mM KCl, 1 mM EGTA, 0.02% Triton X-100
PH: 7.2
Temperature: 37
uniprot:D3ZJH9 [Mg2+]

L_Malate + NADP+ -> Pyruvate + NADPH + H+ + CO2		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM MOPS, 120 mM KCl, 1 mM EGTA, 0.02% Triton X-100
PH: 7.2
Temperature: 37
uniprot:P76558 [Mg2+]

NADP+ + L_Malate -> CO2 + Pyruvate + H+ + NADPH		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl
PH: 7.5
Temperature: 30
uniprot:P76558 [Mg2+]

Pyruvate + NADPH + H+ + CO2 -> L_Malate + NADP+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM MOPS-KOH
PH: 7
Temperature: 30
uniprot:P76558 [Mg2+]

NADP+ + L_Malate -> NADPH + Pyruvate + H+ + CO2		 (( (Vmax * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n))) buffer: 50 mM Tris-HCl
PH: 7.5
Temperature: 30
uniprot:P76558 [Mg2+]

L_Malate + NADP+ -> CO2 + H+ + Pyruvate + NADPH		 (( (Vmax * M) ) / (Km + M)) buffer: 50 mM Tris-HCl
PH: 7.5
Temperature: 30
uniprot:P76558 [Mg2+]

L_Malate + NADP+ -> NADPH + CO2 + H+ + Pyruvate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl
PH: 7.5
Temperature: 30

Pathways

pathway id name
BioCyc:ECO_GLUCONEO-PWY gluconeogenesis I
BioCyc:MOUSE_GLUCONEO-PWY gluconeogenesis I
BioCyc:ARA_GLUCONEO-PWY gluconeogenesis I
BioCyc:ECOL316407_GLUCONEO-PWY gluconeogenesis I
BioCyc:META_GLUCONEO-PWY gluconeogenesis I
BioCyc:BSUB_GLUCONEO-PWY-1 gluconeogenesis I
PathBank:SMP0000839 Gluconeogenesis from L-Malic Acid
PlantCyc:ARA_GLUCONEO-PWY gluconeogenesis I
Reactome:R-HSA-9861718 Regulation of pyruvate metabolism
Reactome:R-MMU-9861718 Regulation of pyruvate metabolism
Reactome:R-RNO-9861718 Regulation of pyruvate metabolism
Reactome:R-HSA-400206 Regulation of lipid metabolism by PPARalpha
Reactome:R-MMU-1430728 Metabolism
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-HSA-70268 Pyruvate metabolism
Reactome:R-MMU-70268 Pyruvate metabolism
Reactome:R-RNO-70268 Pyruvate metabolism
Reactome:R-HSA-1989781 PPARA activates gene expression
Reactome:R-HSA-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-MMU-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-HSA-1430728 Metabolism
WikiPathways:WP5220 Metabolic reprogramming in pancreatic cancer
WikiPathways:WP2621 Glycolysis
PathBank:SMP0120726 Leigh Syndrome
PathBank:SMP0120842 Pyruvate Kinase Deficiency
PathBank:SMP0000334 Pyruvate Decarboxylase E1 Component Deficiency (PDHE1 Deficiency)
PathBank:SMP0120753 Pyruvate Decarboxylase E1 Component Deficiency (PDHE1 Deficiency)
PathBank:SMP0120841 Primary Hyperoxaluria II, PH2
PathBank:SMP0120970 LPS and Citrate Signaling and Inflammation
PathBank:SMP0000196 Leigh Syndrome
PathBank:SMP0000559 Pyruvate Kinase Deficiency
PathBank:SMP0087325 Pyruvate Metabolism
PathBank:SMP0108773 Glutaminolysis and Cancer
PathBank:SMP0000212 Pyruvate Dehydrogenase Complex Deficiency
PathBank:SMP0100046 LPS and Citrate Signaling and Inflammation
PathBank:SMP0120751 Pyruvate Dehydrogenase Complex Deficiency
PathBank:SMP0000060 Pyruvate Metabolism
PathBank:SMP0000466 Transfer of Acetyl Groups into Mitochondria
PathBank:SMP0000558 Primary Hyperoxaluria II, PH2
PathBank:SMP0002298 Glutaminolysis and Cancer
PathBank:SMP0087347 Transfer of Acetyl Groups into Mitochondria