EC: 6.2.1.16

acetoacetate-CoA ligase (acetoacetate:CoA ligase (AMP-forming))

enzyme lambda metabolic reaction experiment
- [K+]

Coenzyme_A + Acetoacetate + ATP -> AMP + Diphosphate + Acetoacetyl_CoA		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris/HCl, 2 mM Phosphoenolpyruvate, 0.25 mM NADH, 2.67 units/ml Pyruvate kinase of rabbit muscle, 9.33 units/ml Adenylate kinase of rabbit muscle, 9.33 units/ml Lactate dehydrogenase of beef heart
PH: 8.4
Temperature: 30
- [K+]

ATP + (S)_3_Hydroxybutanoate + Coenzyme_A -> AMP + (S)_3_Hydroxybutanoyl_CoA + Diphosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris/HCl, 2 mM Phosphoenolpyruvate, 0.25 mM NADH, 2.67 units/ml Pyruvate kinase of rabbit muscle, 9.33 units/ml Adenylate kinase of rabbit muscle, 9.33 units/ml Lactate dehydrogenase of beef heart
PH: 8.4
Temperature: 30

Pathways

pathway id name
Reactome:R-CEL-77111 Synthesis of Ketone Bodies
Reactome:R-DRE-74182 Ketone body metabolism
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-556833 Metabolism of lipids
Reactome:R-MMU-77111 Synthesis of Ketone Bodies
Reactome:R-RNO-74182 Ketone body metabolism
Reactome:R-CEL-556833 Metabolism of lipids
Reactome:R-CEL-74182 Ketone body metabolism
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-HSA-77111 Synthesis of Ketone Bodies
Reactome:R-MMU-74182 Ketone body metabolism
Reactome:R-DRE-1430728 Metabolism
Reactome:R-DRE-556833 Metabolism of lipids
Reactome:R-DRE-77111 Synthesis of Ketone Bodies
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-556833 Metabolism of lipids
Reactome:R-RNO-77111 Synthesis of Ketone Bodies
Reactome:R-CEL-1430728 Metabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-74182 Ketone body metabolism